beauties

12.4.22 Today is the day I start my first grow using @acinfinityinc 2x4 advanced grow tent. I’m doing the cyclops method using @sohumsoils and @foxfarmsoilandfertilizer seed starter. Got my @barneysfarmog amnesia haze from @seedsmangenetics they also gave me Sour D as as the freebie. The two in red solos are randoms I found in some black trufflez in august no clue what’s going happen with them I seen solo cup grows in YouTube so I might try that 12.7.22 Boom three days later we have lift off one of the amnesia haze broke through only one so far. Happy and excited

Update with pics of the end product will follow.

Had some seriously cold nights need to do some insulation work in between rounds it is what it is everything triched up nice gonna chop everything get the next ones in next week everything pretty cloudy now

300g wet, 250g high quality buds other 50g are not in dry weight. “The nightmare is real.” — Rhea Ripley

15.08.: Die zweite Blütewoche hat begonnen und beide Pflanzen haben sich seit der Umstellung gut entwickelt. Da ich vermute, dass die G41 noch weiter wachsen wird, habe ich ein zweites Netz zur späteren Stabilisierung installiert. Das zweite Netz hängt jetzt auf einer Höhe von 1,30m, sodass die oberen Spitzen der G41 bereits durchgucken. Damit die Pflanzen ihre Energie auf die wichtigen Bereiche konzentriert, habe ich bei beiden Pflanzen ein Lollipopping durchgeführt. Dabei hab ich alle Blätter, Seitentriebe und Blütenansätze unterhalb des ersten Netzes entfernt. Diese Blütenansätze liegen so weit unten, dass hier kaum Licht ankommt und sie am Ende stark unterentwickelt sein werden. Idealerweise hätte ich damit noch ein wenig gewartet, aber da ich die kommenden 9 Tage im Urlaub bin wollte ich es vorher noch erledigt haben, das gleiche gilt für das Netz. Updates werden in der Zeit auch eher schwierig, aber vielleicht bekomme ich ein paar Fotos zugeschickt. 18.08.: Ich habe Fotos zugeschickt bekommen. Anscheinend steckt die G41 immer noch in ihrer Stretchphase, da sie das 2. Netz auch schon ein gutes Stück überwachsen hat. 20.08.: Heute kamen nochmal ein kleines Video und ein Foto von der G41. Der Wachstum wird langsam weniger und man sieht, dass sich der Stretch dem Ende neigt. Am Ende der 2. Blütewoche hat die G41 die PBB in Sachen Höhe komplett abgehängt. So wie ich vermutet hab, war der Stretch von der PBB nicht mehr allzu dolle, sodass sie wesentlich kleiner als die G41 geblieben ist. Das ist eins der Probleme, wenn man kein Monocrop anbaut. Außerdem ist der Wasserbedarf der Pflanzen gesunken, sodass die Bewässerung aktuell 10min läuft, wenn das Licht angeht. Nächste Woche bin ich wieder zuhause, dann überlege ich mir, wie ich das Problem löse, entweder mit einer Zusatzbeleuchtung, einem 3. Netz mit dem ich die G41 noch etwas runterbiege oder beides. Da mein Mitbewohner heute vor Beginn von Blütewoche 3 ebenfalls wegfährt, hat er mir gestern noch die aktuellen Daten der Pflanzen zugeschickt. Daher passt es nicht ganz mit dem Diary, ich denke aber, dass es der beste Kompromiss ist.

Apologies folks! I was out of town for the past couple of weeks. Had someone watching over Bruce in my absence, but no progress pictures were taken. Hope you're in for a treat! Lots of pictures this week (may seem repetitive but I was excited lol) even did a quick video under some florescent lights :) Did I mention that I love Main-Lining? Timing couldn't have been more perfect. Before I left the country, I installed the tomato cage and did the final topping, and she was pretty much good to go. At this point, she requires little to no attention until the buds are swole :) Stay Blessed! 🙏👽

This is my fav strain for now. I love the taste more than anything. Take a vape hit and exhale through the nose. Oh god, this is good. Despite the low light intensity, The BUDS ARE TIGHTER THAN THE FIST OF A FIGHTER.

Welcome to Veg Week 3 of Fast Buds Blackberry Auto I'm excited to share my grow journey with you from my FastBuds Project . It's going to be an incredible ride, full of learning, growing, and connecting with fellow growers from all around the world! For this Project , I’ve chosen the Feminized Auto strain Blackberry : Here’s what I’m working with: • 🌱 Tent: 120x60x80 • 🧑‍🌾 Breeder Company: Fast buds • 💧 Humidity Range: 60 • ⏳ Flowering Time: 9-10 Weeks • Strain Info: 23%THC • 🌡️ Temperature: 26 • 🍵 Pot Size: 0.1 • Nutrient Brand: Hy-Pro • ⚡ Lights : 600W x 2 A huge thank you to Fast Buds for allowing me to try my Best with this amazing collection from Automatic and Photo Strains they managed to Sponsore . Big thanks for supporting the grower community worldwide! Your genetics and passion speak for themselves! I would truly appreciate every bit of feedback, help, questions, or discussions – and of course, your likes and interactions mean the world to me as I try to stand out in this exciting competition! Let’s grow together – and don’t forget to stop by again to see the latest updates! Happy growing! Stay lifted and stay curious! Peace & Buds!

Week 9 with video of day 63.

7/13 It's maine-ing out. Overcast and misting. I guess it rained last night but not much. Today is my watering day although I think they could've made it until tomorrow. These drastic temp changes makes it hard to get a STEADY routine in place. I watered everything in the 20's a gallon the 10's 1/2 gallon. The 10th planet in the front was still heavy so I didn't water it. I'll keep an eye on it. Temps are much milder. I noticed a few more septoria leaves on the 10th planet which I promptly defoliated. This pheno is flowering. I could've put this week as flowering as several plants have started. I also saw ONE leaf on my chem dog that had septoria on it. ONE f-ing leaf! I was planning on treating this early anyway so I mixed one gallon of plant doctor 3 tsp gallon/gallon which was the recommendation. I'll monitor this plant and continue treating it the next two weeks. I may do foliar too but the mixture is different. It's a systemic so I don't think that matters as much. Considering getting mulch to protect from spores on the soil. My plants look GORGEOUS. I Hate to use such a nice plant as a guennie pig but I'm not going to sit here and watch it progress. I'm lucky it's really not that bad and that it's on a reveg. There's so many leaves that I would be forced to prune anyway. I'm seeing two MAYBE three leaves a morning but I want to get this under control. Last year u let a plant with septoria go and it finished but went to extracts. I've got a pretty good feeling about this plant doctor. I've used it before with good results. Only time will tell though.🤞 EDIT: WENT OVER AT ABOUT 1PM. ITS 2 NOW AND BLUE SKIES AND SUNNY. IM SUPER GLAD I WATERED AND EVEN MORE SO THAT I APPLIED THE PLANT DOCTOR HOW I DID. THE GIRLS ALL LOOKED AMAZING. THE TENTH PLANET I TREATED WITH PLANT DOCTOR LOOKS THE BEST IVE EVER SEEN IT. I FOUND ON LEAVE DEEP ON THE INTERIOR WITJ A SMALL SPOT BUT ITS SOMETHING IM SURE I MISSED THIS MORNING. IF THIS WORKS THIS GOOD ILL BE APPLYING IT TO EVERYTHING EVEN IF ITS JUST PREVENTATIVE. 7/14 Rained last night andcwas still sprinkling when I went over. Amazingly the 10th planet that I treated with the plant doctorcmix had ZERO visible signs of septoria. I did find one leaf on Mt chem dog and two on my mk ultra with the big leaves. It could've been calcium deficiency but I really doubt it because I think I saw THAT as well. I'm going to wait a day or two and if there are still no negative results and the plant is still looking good I'm going to treat AT LEAST the two others that MAY be infected. It's a systemic and can be used proactively and supposably breaks down into p and k after stimulating immune response. I'll monitor a little longer but as it stands I think I'm going to treat the other two just in case. I also need to start feeding. I've watered a lot more than I normally do so obviously the soil won't last as long. This has been some tricky weather but I'm happy with how things are progressing. I probably should've changed this to the first week of flower as several plants are popping pistols everywhere. EDIT: WENT OVER AROUND NOON AND MIXED UP TEO GALLONS OF PLANT DOCTOR PLANNING TO TREAT THE TWO PLANTS BESIDE THE 10TH PLANET. EVERYTHING LOOKED SO PERFECT AND TOMORROW IS MY WATER DAY SO I DECIDED TO HOLD OFF AND APPLY IT WHEN IT NEEDS WATER ANYWAY. I DID NOTICE THAT PLANTS SEEM TO BE FLOWERING MORE AGGREWWIVELY THAN BEFORE. THINGS ARE GETTING INTERESTING. 7/15 It was water day. The girls looked great this morning. I watered everything at least a gallon and 1.5 for the thirstier plants. I treated my big fan leaf mk ultra and my chem dog #4 with the same Plant Doctor mix as i did tge 10th planet beside it. I havent seen ANY septoria on that plant since application and it looks better than ever. I watered them with a half gallon of phed water before doing a soul drench with the gallon of Plant Doctor @ 3tsp/gal. I'm not seeing much septoria but plants are starting flower and I want to nip it in the bud. This product can be used preventatively and I went by the guidelines for medical marijuana. It was 70 earlier this morning. I'll update as I go forward. EDIT: WENT BACK OVER AT 3 AS THE TENPS WERE PIST 90 AND HAD BEEN ALL DAY. I POSTED A SHORT VIDEO BUT EVERYTHING IS LOOKING GREAT. THE PLANT DOCTOR SEEMS TO HAVE HELPED SUBSTANTIALLY. DIDNT FIND ONE SEPTORIA LEAF. STILL IT WILL TAKE A WHILE TO WORK FULLY BUT IM GLAD I DIDNT CHICKEN OUT AND I APPLIED THE RIGHT AMOUNT. IM GOING TO KEEP AN EYE ON THINGS BUT I MIGHT JUST TREAT EVERYTHING PREVENTATIVELY WHICH IS JUST HALF A TSP A GALLON I THINK. IT DEF TRIGGERS A RESPONSE IN THE PLANT. I LOVE IT WHEN SHIT JUST WORKS OYT AND YOU END UP PLEASANTLY SURPRISED. NEXT WATERING ILL PROBABLY DO NUTES. IM DEBATING WHETBER TO TRELLUS OR JUST USE STAKES AND STRINGS ACROSS THE CAGE AS NEEDED. OH WELL. OVER 90 IS TO MUCH FOR ME TO BE OUTSIDE FOR TOO LONG. 7/16 Plant Doctor is doimg its job. I saw a couple septoria leaves on chem dog but it was interior amd I could've missed it. It takes a little bit to get going. I think I'm going to treat the rest of the garden with at least the preventative dose of 1tsp per gallon. I'll wait and see if this keeps it from popping back up. It almost got to 100 yesterday. It was 80 early this morning. I mixed up two gallons of water and gave the thistiest/lightest a half gallon. I'm hoping that will hold everything over until tomorrow which is water day. I'll have to check but I think it might be time to feed too. I def should make sure to treat tjis septoria before focusing on nutes. It says you can mix it with nutes but I'm leary of doing so. I'd rather apply them at seperate times. I'll watch the garden and of I NEED to water I will. I'm hoping what I did will be enough. EDIT: WENT OVER AROUND ONE. IT WAS 93° WITH SUPER HIGH HUMIDITY. IM GLAD I SPLIT THAT TWO GALLONS WITH THOSE FOUR PLANTS THATS FOR SURE! THEY'D PROBABLY BE DROOPED IF I DIDNT. EVERYTHING WAD PRAYING TO THE SUN GODS. WELL, EVERYTHING EXCEPT THE MK ULTRA IN THE 30 AND THE 10TH PLANET. THEY ARENT DROOPING THEY JUST LOOK NORMAL. THEY ARENT "PRAYING" LIKE THE OTHERS. THEY ARE DEFINATELY A LITTLE LIGHT AND ARE LOOKING FORWARD TO THEIR WATERING. AND THEIR FEEDING. THATS FOR SURE. I WAS SUPER HAPPY WITH WHAT I SAW GOING OVER AND THE GIRLS WILL BE ABLE TO HOLD OUT UNTIL THE MORNING. ONE IN SPECIAL KUSH (WHICH GROW AMAZING AND LARGE HERE) IS IN A 10 AND I EVEN GAVE IT A LITTLE WATER THIS MORNING BUT ITS DROOPY. I COULD STILL FEEL A LITTLE BIT OF MOUSTURE EVEN THOUGH IT WAS LIGHT. IT CAN WAIT UNTIL THE MORNING WITH THE REST. IF IT WAS DROOPING BAD I WOULDA WATERED IT. I DONT GET WHY IM HAVING PROBLEMS FINISHING PLANTS IN 10GAL SMART POTS. YEARS AGO I COULD FINISH SOMETHING IN A 5GAL BUCKET! BUT NOW ITS LIKE THE PLANTS I GROW GET FAR TO BIG TO FINISH FLOWERING ON THESE TINY BAGS. ILL MONITER. HOPFULLY IT WILL BE FINE. IF I HAVE TO I SUPPOSE I COULD CUT THE BOTTOM OFF AND PUT THEM IN THE GROUND OR MORE SUBSTRATE BUT THATS NOT SOMETHING I REALLY WANT TO DO. I CANT WAIT TO SEE YOU THESE GIRLS TURN OUT. 7/17 I'm glad I gave those few plants that extra water. It hit 100 yesterday. Plants made it through it OK. It's 80 at 9am. I watered the garden even though it's supposed to rain with a chance of thunderstorms. Everything got at least a gallon. The mk ultra reveg in the 30 got 2gals as did the thirsty special kush on the end. I've figured out which plants use more water and I try to give them a little more. I also fed today. I was hesitant to feed the plants I treated with plant doctor, but I did anyway. 1tsp grow big 2tsp big bloom .5 tsp calmag. I've noticed pistles coming together on a few plants and forming buds. Next week will definitely be labeled flower. The plants treated with plant doctor LOOK great! I've found a few septoria leaves on a couple other plants. No big deal. I was going to treat the rest of the garden preventatively but if a couple more are infected I'll just add them to the rotation. I just need to make sure I follow-up with the next few treatments. Can't say enough good about this plant doctor. 7/18 Well this fucking sucks. It's 35mph wind gusts and windy as hell. It might've rained last night but if it didn't rain much. I spent the morning "trying" to put up little strong or rope supports to help the girls. I ran a rope across the front of the three on tje back to suppirt them. That one works good. I have one going the other way the entire length which helps support some. Then I took string and ran it around each side of a row for supports. So far no observable damage abd the plants all looked awesome. They've been through a lot and are big tough hardy plants. They'll make it through tjis no problem. I'd be more worried if they had big wet flowers on them but by then I'll have the proper supports and maybe a trellis up. I feel for them this morning and I hope that wind doesn't dry out my bags! EDIT: SPIDY SENSE STARTED TINGLING AROUND ONE SO I WENT AND CHECKED. I GUESS WILL POWER ALONE WONT MAKE IT SO THE PLANTS MAKE IT TO WATERING DAY. WIND GUSTS HAVE BEEN HIGHER THAN 35MPH AND IVE SEEN REPORTS OF 25MPH SUSTAINED WIND. I DONT KNOW WHAT IT IS HERE BUT IT DRIED OUT SOME BAGS AND WAS TURNINGVPLANTS SIDEWAYS. BOTH 10TH PLANETS (THEY DRINK ALOT OF WATER) AND THE MK ULTRA REVEG WERE BADLY DROOPING. THE REST "MIGHT" HAVE MADE IT UNTIL MORNING BUT WHATS THE POINT? I ALSO HAVE TO LEAVE EARLY TOMORROW TO TAKE THE WIFE TO THE DOCTORS. WHAT I DECIDED ON WAS TO WATER THE PLANTS THAT DRINK THE MOST 2 GALS AND GIVE THE OTHERS TGATXWERENT DROOPING AND LIGHT 1.5. I GAVE THE TWO IN THE TENS A HALF GALLON EACH. THERE ATE TWO PLANTS AT THE BACK (SPECIAL KUSH AND PINK KUSH) THAT I DECIDED TO HOLD OFF AND WATER TOMORROW. I NOTICED SOME LEAVES WITH THE COMMON "IF THE BROWN SURROUNDS YOUR PLANT IS DROWNED" SO IF THE PLANTS HAVE A LITTLE WEIGHT, LOOK GOOD AND HAVE PRAYING LEAVES THEN I SHOULD PROBABLY WAIT TO WATER. THEY ARE ALL GETTING BEATEN TO SHIT BY THIS WIND STORM. IM SURPRISED THERE IS NO DAMAGE. THESE PLANTS ARE VERY STRONG. RESILIENT. I DIDNT WATER THE 50 EITHER AND IM UP IN THE AIR ABOUT WATERING IT TOMORROW. Edit 2 I went over around six once the wind died down a little. All the plants I didn't water look great. It's jist the reveges that took it the worst and has some fading leaves. I fixed it with nutes but it doesn't fix the leaves already pale. The reverse mostly are the ones seeing any stress from this storm. The large special kush in the front has buds like an inch long now. Soon it will be time to treat with plant doctor again. 7/19 Well that was a ridiculous windstorm that lasted all day. It was 54 when I was there this morning. I watered the two kush's in the back with a gallon and the 60 with a gallon. Now it's 9 and 70 but it's not supposed to be as hot. This weather is crazy. I defoliated some leaves from the 10th planet that I treated first with plant doctor. Tomorrow it's due for its second treatment. The other plants are only a couple days different and are doing good. Next watering I think I may treat everything. It's a small enclosure. That would be the smart move. I'm having some leaves yellow up on two of the plants that reveged. It sucks because I know I've overwatered a little on occasion but that's because it's 100° with strong wind running through my bags and drying them out. I give them their 10% so its not that I'm not giving enough at a time. I've also noticed some random pest damage. I'm considering using citric acid as I have it on hand and it supposably also helps with septoria. If I do it it will be a diy of Dr. Zymes. The way I look at it is these are outdoor plants. This isn't a sterile environment and shit like this is going tp happen. I saw a leaf that was almost totally eaten. Probably jpn beetle or grasshopper/cricket but they certainly got a good meal! I need to spend more time in the garden. EDIT: WENT over around noon to check things out. Windy and 77°. It LOOKS like the two 10th planet's and the mk ultra reveg are picking up a little. I think they suffered not only wind stress but over watering do to these 100° that can drop to 60° in a day. Those super hot days I even left them until they drooped before I watered but still wasn't good enough. They're in transition though so they use the most water. Plus those 3 are the ones that revegged. It's getting to be close to the one week mark where I do the second treatment of plant doctor. I may hold off a day to get everything on the same page. I'm thinking about treating everything. If some were good I'd guess they wouldn't be after that horrible wind storm. Plants LOOK good though. I defoliated a couple septoria leaves and a few overwatered or yellow leaves.

Vamos familia sexta semana floración de estás Pink Runtz de RoyalQueenSeeds. Que ganas tengo de ver el progreso de esta variedad, las plantas están marcando una deficiencia que estoy ya solucionándola aplicando los nutrientes necesarios, y estos días espero que recuperen el color. La cantidad de agua cada 48h entre riegos. Esta semana ya añadimos nutrientes de floracion estas próximas semanas veremos cómo avanzan. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

Unfortunately what we never want to happen has happened with my baby.. bud rot.. on day 98 of cycle I found 1 bud with bud rot, cut it off, checked the plant and could not find more.. flushed the plant and wait next day to see how it goes. After 2 days main bud was identified with bud rot too.. so sad.. probably due high humidity in my environment + colder weather we are starting to have.. so on day 101 harvest it.. could go few more days to get more amber trichomes but decided to harvest it to avoid lose more buds .. I will keep watching it to see if no spread when drying :(

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Week 5 I am happy with the progress of this plant. She has 4 tops now so it won’t be long until I get her up to the magic number, 8. Things are chugging along nicely so far. Cooler internals: 6.2pH 0.4 EC 65 F solution temp Onward 😎🍻

Most of the day the ladies are under a roof due to rain. Maybe tomorrow a bit of sunshine but the weekend doesnt look good. Like i said another rainy Day,hang in there ladies!

Every week I watch my girls grow into beautiful budding trees only difference is now she in flowering I take her outside to absorb some of the beautiful sunshine and she's loving every bit of it. I let her stay outside for 8 hours in nature then back inside for another 8 hours of led full spectrum and turn out my lights for another 8 hr she's an Auto so she loving every minute of this ...... this is the results ⬆️