2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Gracias al equipo de Royal Queen Seeds, en especial a @james, Marshydro y XpertNutrients y sin ellos esto no seria posible. 💐🍁 Gelato #44: Los criadores de Tyson 2.0 criaron Gelato 44 a partir de cepas finas. Al cruzar la Sunset Sherbet y la Thin Mint Girl Scout Cookies, crearon un híbrido de dominancia índica que asesta fuertes golpes en el cuerpo y la cabeza. Su contenido de THC del 22% crea sinergia con terpenos colocantes para ablandar los músculos Mantenla hidratada y con una dieta constante y pasará por la fase de floración en unas ocho semanas. Las plantas de interior alcanzan una altura máxima de 120 cm y producen 400-450 g/m², mientras que sus homólogas de exterior alcanzan los 180 cm y producen hasta 600 g por planta. 🚀 Consigue aqui tus semillas: https://www.royalqueenseeds.es/rqs-semillas-cannabis-tyson/663-gelato-44.html 💡FC6500: Eficiencia líder en el mercado: la lámpara de cultivo LED FC-E6500, que ostenta un estatus líder en el mercado, es una solución rentable con un PPE de 2,8 µmol/J y un rendimiento máximo de 2,5 g/vatio. Ofrece un PPF alto de 2026 umol/S y es adecuada para una cobertura de vegetación de 1,50 m x 1,50 m y una cobertura de flores de 1,20 m x 1,20 m. Iluminación versátil y uniforme. https://marshydro.eu/products/mars-hydro-fc-e-6500-730w-commercial-led-grow-light/?gad_source=1&gclid=Cj0KCQjw1qO0BhDwARIsANfnkv9IIrYSbmJqz8PqpJOIyWwJfp5bc3SGJgUV68USLQ4tjmXDYwoBuAsaAue3EALw_wcB 🚥 MarsHydro ADLITE UV/IR/RED: Para lograr un crecimiento óptimo de las plantas y maximizar los rendimientos es un arte simple que depende en gran medida de las condiciones ambientales adecuadas. Reconociendo las limitaciones de la iluminación natural y las soluciones de iluminación tradicionales para satisfacer estas necesidades únicas, lanzamos ADLITE. Estas luces especiales UV, IR y roja están diseñadas para llenar áreas del espectro, proporcionando las altas longitudes de onda que las plantas necesitan para un crecimiento y desarrollo óptimos. 🚀 Consigue aqui tu Adlite: https://marshydro.eu/collections/adlite-supplemental-lights/ 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 7: Equivale a la semana 3, ya que el con el error que tuve en el cambio ellas no florecian. Los cogollos comienzan a formarse y por fin se detiene su crecimiento. La resina empieza a estar presente en la hojas.

Been a busy week sorry fir the late update everything is going great she is still stretching not as much as o would of liked hopfully by the end of this week she will have that final push

Vamos familia, volvemos con las candy caramelo y es que pronto nos pedirán un trasplante y con este caos veremos cómo conseguimos sustrato. Ph controlado a 6,5 siempre hay que controlar este parámetro. Temperatura y humedad correcta En la 2 semana de crecimiento. Os dejo la web y el código para compras en mars hydro. web: http://bit.ly/2uJAjgy ts600: http://bit.ly/3cnv0Ev code: an420 Ya tengo ganas de ver como florecen estas niñas en las próximas semanas las vemos un saludo fumetillas.

Day 64 (First Grow, Flowering Phase): Hey everyone! We’re on day 64, and the flowering phase is in full swing. The buds are forming beautifully, and the plants have grown 5-10 cm above the SCROG net, creating a lush canopy. The humidity is steady at around 50% or lower, which is ideal for this stage. To recap, we've been maintaining an even canopy with the SCROG net and our Timor shield mantis continues to keep pests at bay naturally. Our plants are thriving, thanks to the even light distribution and careful humidity control. We're considering installing a second fan in a few weeks to improve airflow and potentially adding a second SCROG net to support the plants as they continue to stretch during flowering. This should help stabilize the plants and ensure they get the support they need as the buds get heavier. Any tips or advice for the next steps? Let us know in the comments!

The Titan is growing beautifully and the smell keeps getting better by the day. This is the first time using an Airpot to grow and I noticed the plant does need more water compared to a Geotextile pot. Luckily I can check up on her a few times a day and give water when needed. Not much to do in terms of training as this lady practically trained herself ¯_(ツ)_/¯ I add updated daily instead of at the end of the week, so more pics and video's coming up in the next few days.

Shes just trucking along as batman has said before all I do is water her with Ph water and that's it all the nutrients were added to the soil so shes taking care of all that by herself. Batman cant wait to see some real bud growth hoping for a nice harvest considering shes just a single plant

Buds should be dried in the dark at 20 degrees in 50% humidity 👌

Goingto start LST this week everything is going well 💯 HAPPY

Week 5 of flower Everything seems fine,so far Looking healthy Another 3 weeks to go Stay Safe everyone!

Please like and follow the diary, or leave a comment!! appreciate the support guys🙏💚🔥 Ok guys here we are i thought they would be ready for harvest by now, but i think they gave another 7-9 days to go soo fingers crossed next post will be harvest time! I would have carried on feeding if I knew it would take this , but now we are on flawless finish until harvest Trichomes are still mostly milky, have some clear ones on the banana purple punch because she is foxtailing like mad because of heat, and just a touch of amber on some Been 30+ Celsius most of the grow, luckily this week will be cooler for them I also did a stupid and fell in my tent while taking pics, ended up with 1 branch of z up as a premature tester hahaah I've added some pics of it Am going to enter my banana purple punch and z up in the biggest cola contest (I know my ruler starts from 22cm my 10 month old broke it) so BPP is currently 34cm long and the z up is 24 cm long

Bonjour à tous, ces derniers jours ce sont bien passé pour mes petit bébés elles grandissent doucement mais sûrement.C’est la première fois que je travail avec cette gamme de produits (je vous est mis des photos des produits 😉) pour l’instant j’en suis satisfait après, il faut voir le résultat final ce qui est moins facile 😂. Mes petit bébés commence leurs 5ème semaine (je me suis trompé d’une semaine 😕 désolé 😉) trop pressé!!! 😂 de mettre de nouvelles photos 😜. Je vous dis à là semaine prochaine vivement 😉 bonne journée à tous. Je vous remercie 😉.

Week 12 the Apple fritter still growing really good no damages or defects of any kind got lots of white hairs that have shot and I believe it is in its first real week of transition. Looks like it’s gonna have a big stretching week and we’ll see about having some little popcorn buds in the next week or so first big transitional week. The apple fritter is over 8 feet wide and over 8 feet tall Was really really growing fast right now. I expect it to finish around 9 x 9 excited to see some giant apple fritter cola’s.

Ladies and gentlemen, day 66 is here and as you can see, she has a long way to go... but she has had one heck of a stretch and the internodal spacing is awesome, so i'm just going to let her go gonna be probably another 2 months before she gets the axe because I really want these buds to swell up big and fat ... Big shout out to Zamnesia Seeds and Plagron for making this grow possible and inviting me to participate in their awesome contest.. other breeders, take notes.This is how you do a contest 👌 ... i will update throughout the week if need be and if not, I'll be back in 7 days like clockwork to update my diary ... thanks, everybody stopping by and showing love.It is much appreciated.. God bless everybody good luck to all of those in the contest and to those that are just growing for the love of growing , or for the need for this medicine, good luck to you as well.. Growers love guys ✌️

It was fun to grow in the back yard. I wouldn’t grow again though. They get stressed and stunted very easily. Don’t smell. Low yield. Low potency.

Die dümmsten Bauern haben die dicksten Kartoffeln. Die dicksten Buds hab ich nicht, aber dafür viele 😄 129gr Buds und 22gr Popcorn. Bin auf das curing gespannt, der Geruch ist jetzt schon verlockend. Die Wirkung brauch nen Moment, ist dann erst kopflastig und kommt so nach 30 Minuten entspannt body-lastig.

nutrients are the same as in the weeks before and dont change as mutch. she is very fast if this holds up so good this is my first hydro and f1 at the same time so pretty glad all works so well.

👅🤑💦