First full week of flowering and they are finally on AC Infiniti’s self watering bases. Switching to CRONK Nutrients.

Another week down I think I've already defoliated before about to bit em again this strain can take alot of stress as with most mephistos I've grown.

As you can see, my leafs don't look really healthy. It's either the fact that the soil was too wet for a couple of days cause my friends took care of it when I was away or it's because the humidity is so low. It's only on 36%, even though i bought an humidifier. The light was maybe too close to the plant as well in first week (30 cm I think, maybe less). I already highered the lamp to try and lower the humidity and to prevent the leaves from curling but idk if that's the solution... My first grow I never had problems with my humidity, maybe its because its colder outside now? I changed my ventilator as well, maybe that has an impact as well, but surely not that big? Any tips on: -What I should do to improve the leafs and prevent them from curling -What I should do to higher the humidity. As for nutrients, I mixed my soild with Biogrow (3g/l) when the plant was around day 3. I think this may have been too much too soon, but that's what they adviced on their site so I just went with it and afterwards I found out that you should try to wait untill your plant is a bit bigger.(I think)

steady as she goes.

Grape ape on week 2 flower here waiting on other hybrids to catch

Nothing much yet.

Impressed with the quality of the buds rock solid & the smell a mix between strawberry pear & peach🍓🍐🍑super sweet! Easy trim✂️ Two different Pheno The green Pheno is more sweet and the purple more sour🤤 Now 7-10 days on the dry rack then see you in 2025 for the smoke review Take care 😎

Doing some LST

Bonjour à tous les padawans et maîtres jedis Super plante ou plutôt jolie petit arbre avec de branches bien chargées de grosses têtes remplis de trichomes Un vrai plaisir à cultiver très belle et résistante

Just been chopped toon al the lower branches off and put them away separate but will update on the total yield

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

These lights are a bit hot so I’m gonna upgrade to my Luxx 645s for the rest of this run.

Things are still looking good, being aggressive with the hydroguard

Week 7 – The Pest is Dead Found the source of the whiteflies – two infested CBD plants I’d placed in the tent. Removed them, and since then only a few flies on traps. Problem solved. Buds are swelling nicely with more trichomes and orange pistils. Some yellowing leaves toward the end of the week, likely natural fade. Watered twice with pH water and gave one light HESI feed (½ dose) on Sunday. Dimmed light from level 7 to 6; PPFD is ~800 top / ~550 lower. Everything’s on track!

***Video Added March 14th*** March 13, 2019 Update: We’re getting to the end of the road here. DinaMed and all three Cherry’s were cut down on Saturday and hanging. The Critical+ and Cream & Cheese CBD are flushing with straight water and will be cut down this weekend. I’ll be taking samples of the DinaMed and Cherry to the local dispensary for potency testing this weekend. Pics were taken both with lights on and lights off. I used a flash when the lights were off. The first round of pics are with the HPS off and the flash on, the second round is with the HPS on and the flash off. I probably won't update this diary again until I have the test results back and final dry weights for all plants - 10 days or so. 🙏 p.s. I'm starting a couple new diaries next, check em out! 👍

This plant was amazing from start to finish. I will update a final comment soon as well as final weigh and a review of the smoke once sent out to a group of testers.

7/14 4AM MONDAY MUST DEFOLIATE!!!!! I HAVE 4 INSTEAD OF 2...BECAUSE OF MY LITTLE ACCIDENT EARLY ON.. I WILL TAKE ONE OUT OF FLOWER AND RE-VEG TODAY!!! 7/15 420am They are great cutting I took last month or week is great. Amino and mb today!!

This thing been a game