I always find this the tricky week. When the 2nd set of real leaves are out, and it's in a cup. Every 3 days, I flipped all my cups upside down, tapped them out gently, and checked the roots.
If the soil falls away, too dry, if you see no good micro-roots spidering on the edges, too moist. IMHO, Ignore anything but the roots at this size. By end of week 3, I have 14 now in 2.5qt pots stretching into the sunshine; with maybe 8-10 with weak root systems still in the tent. Love seeing them grown in dirt. Best wishes. MrCrispy