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1. The workbench and all gears were cleaned with water. Then sprayed and wiped with 99% isopropyl alcohol to sterile.
2. A 400ml of 0.5% H2O2 solution was made by diluting 4ml of 50% food grade H2O2 with 396ml of Reverse Osmosis (RO) water.
3. The biggest seed with the darkest color was selected.
4. Seed was submerged into 15ml of 0.5% H2O2 solution in a corresponding glass jar for 12 hours to soften its shell and prevent it from bacterial attack.
5. A Root Riot cube was also submerged into 0.5% H2O2, but in separate container. It was submerged for 12 hours to sterile.
===== 12 HOURS LATER (12/13/23 11:16 PM) =====
6. Germinating temperature is recorded at 21.7c. RH is irrelevant since a humidity dome will be used.
7. Root Riot cube is picked out off the H2O2 bath and drained off excess liquid by shaking (!!!NEVER SQUEEZE THE CUBE!!!).
8. Seed is sowed ~0.5cm into the opening hole of Root Riot cube.
9. The cube is placed into the tray and labeled along with two other seeds.
10. Humidity dome is put on with all ventilation holes closed.
===== 72 HOURS LATER (12/16/23 10:56 AM) =====
11. Seed reoriented itself. Good sign. Expect a sprout within 24 hours from now.
===== 96 HOURS LATER (12/17/23 10:35 AM) =====
12. Young sprout is supplied with 120 µmol/m2/s of light from two T5 LEDs under 24/0 schedule.
13. Humidity dome is opened 10%.
===== ENTERING EARLY VEGETATIVE STAGE (12/30/23 08:35 PM) =====
1. Light intensity is increased to 350 µmol/m2/s (DLI: 30.24 mol). Light height 8 inches.
2. CO2 intensity was increased to 800 PPM.
3. Climate is recorded at 29 degrees Celsius, 68% RH, 2090 PPM CO2.
4. Grow system flushed and replaced with 0.01% H2O2-treated vegetative strength nutrient.
===== (01/02/24) =====
5. Nutrient parameter is recorded at 570 PPM, pH 5.75
6. Plant topped at the 5th node.
===== (01/02/24 10:58 PM) =====
7. Light intensity is increased to 406 µmol/m2/s (DLI: 35.08 mol). Light height 8 inches.
8. Climate is recorded at 29 degrees Celsius, 63% RH, 1235 PPM CO2.
9. Nutrient parameter is recorded at 570 PPM, pH 5.8.
10. CO2 intensity was increased to 850 PPM.
===== (01/03/24 12:58 PM) =====
11. Nutrient parameter is recorded at 560 PPM, pH 6.0.
===== (01/05/24 12:11 PM) =====
12. Nutrient parameter is recorded at 610 PPM, pH 6.1.
13. Light intensity is increased to 465 µmol/m2/s (DLI: 40.18 mol). Light height 8 inches.
===== Entering WEEK 2 of EARLY VEGETATIVE stage (01/07/24 3:51 PM) =====
1. Nutrient parameter is recorded at 610 PPM, pH 5.8.
===== 01/08/24 12:55 PM =====
2. Start Low Stress Training.
3. CO2 intensity adjusted to 900 PPM.
===== 01/09/24 09:29 PM =====
4. CO2 intensity adjusted to 950 PPM.
5. Nutrient parameter is recorded at 620 PPM, pH 6.1.
===== 01/10/24 08:42 PM =====
6. More LST.
7. CO2 intensity adjusted to 1000 PPM.
8. Nutrient parameter is recorded at 650 PPM, pH 5.8
===== Entering WEEK 1 of LATE VEGETATIVE stage (01/12/24 10:09 AM) =====
1. CO2 intensity was raised to 1300 PPM.
2. System was flushed and refilled with 0.01% H2O2-treated late vegetative strength nutrient solution.
===== 01/13/24 03:04 PM =====
1. Nutrient parameter is recorded at 710 PPM, pH 5.7.
===== 01/15/24 06:05 PM =====
1. Nutrient parameter is recorded at 740 PPM, pH 5.8.
===== 01/17/24 11:48 AM =====
1. More pruning and LST.
2. Nutrient parameter is recorded at 800 PPM, pH 5.6.
===== 01/18/24 02:23 PM =====
1. More pruning and LST.
2. Nutrient parameter is recorded at 840 PPM, pH 5.9
===== 01/19/24 08:43 PM =====
1. More pruning and LST.
2. Nutrient parameter is recorded at 870 PPM, pH 5.8
===== ENTERING WEEK 2 OF LATE VEGETATIVE STAGE 01/20/24 =====
1. More LST and pruning.
2. Nutrient parameter is recorded at 900 PPM / pH 5.9.
===== 01/21/24 08:27 PM =====
1. Nutrient parameter is recorded at 920 PPM / pH 6.0
2. ScrOG net is placed.
===== 01/22/24 06:19 PM =====
1. Nutrient parameter is recorded at 950 PPM / pH 5.7
2. 1 gal partial reservoir changed.
===== 01/23/24 09:41 PM =====
1. Nutrient parameter is recorded at 960 PPM / pH 6
===== 01/25/24 11:22 AM =====
1. More scrogging and pruning.
2. Nutrient parameter is recorded at 940 PPM / pH 6
3. Light intensity was adjusted to 520 umol/s/m2 (44.93 mol).
===== 01/26/24 05:29 PM =====
1. 1 gal partial reservoir changed.
2. Nutrient parameter is recorded at 940 PPM / pH 5.9
===== ENTERING WEEK 3 OF LATE VEGETATIVE STAGE 01/28/24 08:29 PM =====
1. More pruning and scrogging.
2. Nutrient parameter is recorded at 1000 PPM / pH 6.1
===== 01/29/24 06:11 PM =====
1. More scrogging.
2. Nutrient parameter is recorded at 980 PPM / pH 6.1
===== 01/30/24 02:38 PM =====
1. Full reservoir changed and topped off with 0.01% H2O2-treated late vegetative strength nutrient solution.
===== 01/31/24 06:34 PM =====
1. Nutrient parameter is recorded at 930 PPM / pH 5.8
===== 02/01/24 10:55 AM =====
1. Nutrient parameter is recorded at 920 PPM / pH 5.8
2. Light schedule is toned down to 23.2/0.8 to limit the DLI to 41.5. mol.
===== 02/02/24 10:02 PM =====
1. Nutrient parameter is recorded at 950 PPM / pH 6
===== 02/03/24 11:42 PM =====
1. Nutrient parameter is recorded at 990 PPM / pH 6.1
Saw this unhappy plant and read your grow conditions. Just some notes for you:
- Give the plants some dark, not 24 hrs of light
- 29c is too hot for a seedling
- Light is way too strong for this phase
- Co2 is unnecessary until late veg/early bloom
Good luck