Hi, I've harvested in stages a few times, mainly because the lower buds are often not as ripe as the upper ones. And I don't want too many amber trichomes on the upper ones, I've never had any problems; at this point, your humidity should be pretty low anyway to prevent mold in the buds, so there shouldn't be any issues where you cut. If you're worried about them being hermies, I've never had that either, especially since there's usually only a week between the first and second harvest, and they don't develop seeds that quickly.