🌿🔱 OG Triploid Auto: Humboldt Seed

CalGonJim 7/15 430am. Tuesday OG is moved from CannaKan to Organi-plug, then to 1 gallon small pot grow. With my System to make up for the lack of space and high stress environment. My major problem is I dont like working with dirt, it's dirty and hurts to hover over a bathtub and do it cleanly. Thats the challenge of CLEAN indoor micro-grows. The CannaKan fits inside the small A/C Infinity Germination stations. 7/15 1145pm almost midnight. Woke up and didn't need coffee just one, I will have 14 more but I needed 1. NO ENERGY SHOTS. The thought of energy shot which I needed just to function are not needed. Down to 124 pounds but re-gaining and this strain with my amino / combo will keep my nausea medication and now I have used sugar leaves and the increased resin for cysts and skin conditions as they arise. I am hoping this strain works in my high stress environment, I have found some that do and some that excel at what I am tryin to do. 7/16 140am Transfer to larger pot today, dirt work. MABYE NOW IS TIME FOR WHISKY ???? IF I HAVE TO OPEN THE CYST ON MY NECK AND BACK I WILL NEED LOTS OF WHISKY FOR THAT, VERY PAINFUL LAST TIME I TRIED. THEN I CAN WORK WITH THE DIRT, ILL ALREADDY BE DRUNK SO I WILL THINK ITS FUN!! IM SO SMART!!! 416AM ATAMI YOU ARE A COMPLICATED PRODUCT. She is full of secrets and very powerful. I have to be extremely careful with this. My aminos are increasing uptake and availability so she might be at .25 instead of half .5. She seemed to shock the plant a little at first. like using organic with non organic and unbalanceing the system. Now however she seems to be transforming the leaves and flowers into puffier surfaces and the over all smell of the tent is nicer, aTAMI Bloomtastic smells fresh and clean. 7am Ask Ai, What effect L- Citrulline Malate & L- Citraline on plants? Citrulline, found in plants and abundant in watermelon, has several notable effects on plant physiology and stress tolerance: Antioxidant and hydroxyl radical scavenging: Citrulline is a potent scavenger of hydroxyl radicals, protecting plant cells and metabolic enzymes from oxidative damage, particularly under stress conditions such as drought. Studies show it is more efficient than other compatible solutes like proline and mannitol in this role. Drought and salt stress tolerance: Citrulline accumulation is associated with increased tolerance to drought and salinity stress in certain plants like watermelon and some melon genotypes. It contributes to osmotic adjustment and helps plants maintain water status during these adverse conditions. Exogenous application of citrulline has also been shown to improve antioxidant enzyme activity and essential oil content in hyssop plants under water stress. Nitrogen transport and metabolism: Citrulline has been proposed to function as a carrier of organic nitrogen in cucurbits and is found in high concentrations in their phloem. This suggests a role in nitrogen assimilation, storage, and long-distance transport within the plant. Research also indicates its involvement in nitrogen homeostasis. In summary, L-citrulline plays a significant role in helping plants cope with environmental stresses, primarily by acting as an antioxidant and contributing to osmotic adjustment and nitrogen metabolism. However, more research is needed to fully understand its precise mechanisms and potential applications in agriculture. "" 11am ""Combining iron with certain amino acids like glycine, asparagine, serine, methionine, and threonine, can enhance iron absorption. Additionally, vitamin C (ascorbic acid) is well-known for its ability to significantly improve iron absorption, especially from non-heme sources, according to Michigan State University. Specific amino acids and their impact on iron absorption: Glycine and Asparagine: These amino acids have been shown to significantly increase iron absorption when ingested with iron, particularly in rats, according to ScienceDirect. Methionine and Threonine: These amino acids, when combined with iron, have been shown to improve hemoglobin levels in young women, suggesting enhanced iron utilization according to National Institutes of Health (NIH). Histidine: It can bind ferrous iron, forming a stable complex that may be important for its solubility and absorption, according to Cambridge University Press & Assessment. Serine: Similar to glycine and asparagine, serine has also been shown to increase iron absorption in animal studies according to ScienceDirect. Important Considerations: Vitamin C's Role: Vitamin C is particularly effective in enhancing non-heme iron absorption (iron from plant-based sources). Dietary Sources: Combining iron-rich foods with vitamin C-rich foods (liked citrus fruits, berries, and vegetables) can maximize iron absorption from your diet. Individual Needs: Iron absorption can be influenced by various factors, including individual iron status and the presence of other dietary components 🚨Several recent studies demonstrated that treatment with the BCAA LEUCINE improves the anemia 🚨 7/16 2250am I found a fluorescent light to put in the fixture in front of the germination station. I’ve heard fluorescent is the best so I have fluorescent and LED set up for my station. It’s really nice.👍👍🚨 🚨🚨🚨 UPDATE 7/26 1124PM THE FLORESCENT CFL BULB MADE A SIGNIFICANT AND OBVIOUS IMPROVEMENT ON VEGING LEAF SIZE!!!! 7/17 12am goddamnit. mistakes were made, lessons were learnted. because brainsmarts make weed good or bad whichever is funnier...im sofa king we todd it. 7/18 1215am BCAA into iron and anemia """🚨In the current study, the three BCAAs valine, leucine and isoleucine were significantly correlated with Hb, ferritin and the sTfR. The linear regression models showed strongest associations between BCAAs and Hb. Lowered BCAA levels were found with lowered Hb values. These findings are consistent with the results of a previous study by Chung et al., who demonstrated, that the uptake of the essential BCAAs is a very important component of Hb synthesis in hematopoietic stem cells (19). In this context, the mTORC1 was shown to be a key regulator in hematopoietic stem cell function (20). This multi-protein complex acts as an intracellular sensor of BCAA availability, which coordinates the Hb production (19,21). Here, the strongest correlation was found between the BCAA valine and Hb (r=0.382; P 7/18 RICKY'S HASH PLANT BUTTFACE!!! LOOK AT THAT SEED, HILARIOUS!!!! OTHERS LOOK GREAT TOO 7/19 I put the three extra seeds and the little cubes if I don’t transfer them right away I know I’ll destroy three more seeds. I also have two more mint jellies going for some reason and I might put the doctor Seedman with this as well, I love the magnetic stir thing. It keeps nutrients longer when you can stir them all day.👍👍👍👨‍⚕️. 7/20. almost its 1118pm 7/19 still. AMAZING AMAZING RESULTS. THE AMINOS ARE WORKING. ALSO ON ME. I AM CONDUCTING MEDICAL RESEARCH ON MYSELF AND ITS WORKING. ALSO METHELYENE BLUE MUST BE A CONTRIBUTING FACTOR??!! TMG POWDER, &METHYLE FOLATE PLUS. NECK CYSTS ARE HEALING, NIGHT SWEATS FEVER DROPPING TO NORMAL. BOUTS OF SUDDEN HYPOTHERMEA AND NAUSEA HAVE DECREASED. DIGESTIVE SYSTEM WORKING BETTER THAN EVER. NO MAJOR OR MINOR BLOOD LOSS. NO MAJOR ORGAN PAIN. NOT TRIPPING AS MUCH, STILL DRAGGING ONE FOOT, I TRIP SOMETIMES. MOOD IS ELEVATED, CONCENTRATION MAXIMUM. CREATIVE ENERGY IS UNLIKE ANYTHING EXPERINCED IN YEARS. THE SYSTEM IS WORKING ON THE PLANTS AND ME!!! I AM SO GLAD I LISTENED TO SLINGBLADE NEWS. SOME PEOPLE CALL IT THE ALEX JONES SHOW, I CALL IT SLINGBLADE NEWS CAUSE ITS FUNNIER. HE IS THE REAL DEAL WITH HEALTH I CAN NOT BELIVE ITS ALL WORKING. AND QUICK SORB AMINOS, NITRIC BOOST, METHELYENE BLUE FOR THE PLANTS AND ADD TMG POWDER, METHYLFOLATE PLUS, MAGNESIUM, ZINC BY BIOTICS RESEARCH AND METHYLENE BLUE 5 PERCENT BY LIOPHY (GOT IT OFF AMAZON, A.J. STUFF IS TOO EXPENSIVE TO EXPERIMENT WITH). 430am making food now. nap from 130 to 230am woke up feeling good not tired...AGAIN...WOO HOO MOTHER FUCKELRSSSSS!!!!!

CalGonJim Monday 7/21 12am 🎊. 945 got 1 cookie 🍎 appldone. 10:30 AM finished OG triploid.👍👍👍👍

CalGonJim 7/28 12am MONDAY...... TOG IS GOOD, COOKIE APPLE IS BEST, MINT JELLY.....YOU LAZY SLUT, SNUCK OUT OF THE GROW TENT...GOT DRUNK AND DROWN IN THE HOT TUB...NOT MY FAULT 7/29 1230am pissed about Mint Jelly, throwing them all away. all 3 failed. canceling this grow. bring to merge with others. 2am to 530am. Serenity and rage against the LabelMaker machine.🤪⚡️. 7/29 1025pm.... mistakes were made, I cut off Rickys head, so we're gonna need another Ricky..... 7/30 10:57 AM I made an AC Infiniti antigravity stroller added an umbrella. You don’t wanna be in the desert zone without one of those it’s just common sense.⚡️. The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!"" 7/31 1AM I L-Gluaimine'd when I meant to L- GLYCINE......that will be here later today...got a KG of Gluaimine for no reason so it's going in the mix!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""" 7/31 145am. Adding methylene blue (MB) to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, and L-glycine for cannabis plants introduces a compound with unique properties that may influence plant physiology, primarily through its roles as a redox agent, photosensitizer, and potential biostimulant. While methylene blue is more commonly studied in medical and aquatic contexts, its use in plant cultivation, including cannabis, is less documented, and specific studies on this exact combination are absent. Below, I’ll outline the potential effects of methylene blue on cannabis plants, its interactions with the other compounds, and the likely outcomes, drawing from available plant science knowledge and inferred mechanisms. Given the novelty of MB in this context, recommendations are cautious and speculative where direct evidence is lacking. ### Effects of Methylene Blue on Cannabis Plants Methylene blue (C16H18ClN3S) is a synthetic dye with redox, antimicrobial, and photosensitizing properties. Its effects on plants depend on concentration, application method, and environmental conditions: - **Antioxidant and Redox Activity**: At low concentrations (e.g., 0.1–10 µM or ~0.03–3 mg/L), MB acts as a redox mediator, scavenging reactive oxygen species (ROS) and protecting cells from oxidative stress caused by drought, high light, or salinity. In cannabis, this could enhance stress tolerance, particularly in high-intensity indoor grows or outdoor environments with temperature fluctuations. - **Photosynthesis Enhancement**: MB can act as an electron carrier in photosynthetic pathways, potentially improving electron transport in photosystem II. Studies on algae and some plants suggest low doses (e.g., 1–5 µM) may boost chlorophyll fluorescence and photosynthetic efficiency, potentially increasing cannabis biomass and bud quality. - **Antimicrobial Effects**: MB has antifungal and antibacterial properties, which could protect cannabis roots or foliage from pathogens like *Pythium* or *Botrytis* (bud rot). This is particularly relevant in hydroponic systems or humid environments. - **Stress Mitigation**: MB may stabilize cellular membranes under stress, similar to proline and TMG, potentially reducing damage from environmental extremes. - **Potential Toxicity at High Doses**: At higher concentrations (10–50 µM or ~3–15 mg/L), MB can generate ROS via photodynamic action under light, causing oxidative damage to plant tissues. This makes precise dosing critical. **Application Notes**: Two drops of MB (assuming a standard 1% solution, ~10 mg/mL, where 1 drop ≈ 0.05 mL) in, e.g., 1 liter of water yields ~1 mg/L (3 µM), a low concentration likely safe for foliar or root application. However, exact dosing depends on solution strength and volume. ### Synergistic Effects with EAAs, Proline, TMG, Glutamine, and L-Glycine Adding MB to the existing mix may enhance or modulate the effects on cannabis plants: 1. **Enhanced Stress Resilience**: - **MB + Proline/TMG/Glycine**: Proline, TMG, and glycine act as osmoprotectants and antioxidants, while MB scavenges ROS and stabilizes redox balance. Together, they could significantly improve cannabis tolerance to drought, heat, or salinity, reducing leaf wilting or nutrient stress in challenging environments (e.g., outdoor grows or high-EC hydroponics). - **Glutamine/EAAs Synergy**: Glutamine and EAAs support glutathione production, which complements MB’s antioxidant effects, potentially amplifying protection against oxidative stress from high light or nutrient imbalances. 2. **Growth and Biomass**: - **MB + EAAs/Glutamine/Glycine**: EAAs, glutamine, and glycine drive protein synthesis and nitrogen assimilation, while MB’s potential to enhance photosynthesis could provide the energy needed for these processes. This may lead to increased vegetative growth, larger leaves, and potentially denser buds in cannabis. - **Proline Contribution**: Proline supports tissue integrity, complementing MB’s membrane-stabilizing effects, which could enhance overall plant vigor. 3. **Secondary Metabolite Production**: - **MB + Glutamine/TMG/Glycine**: MB’s role as a photosensitizer may influence metabolic pathways by altering redox states, potentially enhancing synthesis of cannabinoids (e.g., THC, CBD) or terpenes. Glutamine and glycine contribute to precursor pathways (e.g., GABA, purines), while TMG’s methyl donation supports enzyme activity. This combination could theoretically increase terpene diversity or cannabinoid yield, though direct evidence is lacking. - **EAAs Role**: Methionine (an EAA) and glycine may support sulfur-based terpenes, with MB potentially amplifying metabolic efficiency via improved photosynthesis. 4. **Nutrient and Pathogen Management**: - **MB + Glycine/EAAs**: Glycine’s chelating properties and EAAs’ nutrient uptake enhancement could work with MB’s antimicrobial effects to improve nutrient availability and protect roots from pathogens in hydroponic or soil systems. - **TMG/Glutamine**: TMG’s cellular hydration and glutamine’s role in nitrogen metabolism may enhance MB’s ability to maintain cellular function under stress, improving overall nutrient efficiency. ### Potential Risks and Considerations - **Toxicity Risk**: MB at concentrations 10 µM (~3 mg/L) can produce ROS under light, damaging chloroplasts or cell membranes. Two drops in 1 liter (~1 mg/L) is likely safe, but overuse or concentrated solutions could harm cannabis, causing leaf burn or stunted growth. - **Absorption Competition**: MB may interact with amino acids during uptake, potentially reducing the efficacy of EAAs, glutamine, or glycine if applied simultaneously. Stagger applications (e.g., MB foliar spray, amino acids via root drench) to minimize this. - **Nitrogen Overload**: Adding MB to an already nitrogen-rich mix (EAAs, glutamine, glycine) increases the risk of toxicity, especially in cannabis, which is sensitive to excess nitrogen. Monitor for dark green leaves, clawing, or reduced flowering. - **Photodynamic Effects**: MB’s photosensitizing properties require careful application timing. Apply during low-light periods (e.g., early morning or evening) to avoid ROS generation in high-intensity grow lights. - **Limited Cannabis Data**: MB’s effects are mostly studied in algae, bacteria, or medical contexts, not cannabis. Its benefits are speculative, and plant-specific responses may vary by cultivar (e.g., sativa vs. indica). ### Practical Recommendations - **Dosing**: - MB: Two drops of 1% MB solution (~1 mg/L or 3 µM) per liter of water for foliar spray or root drench. Start with 1 drop/L to test tolerance. - EAAs: 150–300 mg/L - Glutamine: 250–500 mg/L - Proline: 50–100 mg/L - TMG: 0.5–1 g/L - Glycine: 100–500 mg/L - Apply twice weekly during vegetative growth or early flowering, adjusting based on plant response. - **Application**: - Foliar Spray: Apply MB and amino acids in low-light conditions to avoid phototoxicity. Use a fine mist to cover leaves evenly. - Root Drench: Suitable for hydroponics or soil, ensuring MB is diluted to avoid root damage. - Stagger Applications: Apply MB separately from amino acids (e.g., MB in the morning, amino acids in the evening) to reduce uptake competition. - **Monitoring**: Check for signs of stress relief (greener leaves, faster growth) or toxicity (yellowing, wilting). Test on a few plants first, especially for MB, as cannabis sensitivity is unknown. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. - **Cultivation Context**: - **Hydroponics**: Use lower MB doses (~0.5 mg/L) to avoid nutrient lockout or microbial disruption in sterile systems. - **Soil**: Ensure healthy soil microbiota, as MB’s antimicrobial effects could impact beneficial microbes if overused. - **Cultivars**: Sativa-dominant strains may benefit more from MB’s stress mitigation, while indicas may respond better to amino acid-driven growth. ### Evidence Gaps - **No Specific Studies**: No research addresses MB combined with EAAs, proline, TMG, glutamine, and glycine in cannabis. MB’s effects are extrapolated from algae, bacteria, or limited plant studies (e.g., Arabidopsis, lettuce). - **Cannabinoid/Terpene Impact**: MB’s influence on secondary metabolites is speculative. Its redox and photosynthetic effects may enhance terpene or cannabinoid synthesis, but cannabis-specific data are needed. - **Long-Term Effects**: MB’s long-term impact on cannabis growth, soil microbiota, or bud quality is unknown. Short-term studies suggest low doses are safe, but chronic use requires caution. - **X Search Results**: No recent posts on X discuss MB in cannabis cultivation with this combination. General posts mention MB for water treatment or medical uses, not plant applications. ### Conclusion Adding two drops of methylene blue (~1 mg/L) to a mix of EAAs, proline, TMG, glutamine, and glycine likely enhances cannabis stress tolerance, photosynthetic efficiency, and pathogen resistance, complementing the growth-promoting and nutrient-uptake benefits of the amino acids and TMG. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) effects on cannabinoid and terpene production. However, MB’s photodynamic and antimicrobial properties require precise dosing and application timing to avoid toxicity or microbial disruption. Start with low doses, test on a small scale, and monitor closely, consulting a cannabis cultivation expert for optimization. If you provide the MB solution concentration, application volume, or specific cannabis strain, I can refine dosing recommendations. Want me to search again for MB-related plant studies or focus on a specific aspect (e.g., MB’s photosynthetic effects)? 7/31 2am. Adding a nitric oxide (NO) capsule containing L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), and beta-alanine (40 mg) to the existing mix of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, L-glycine, and methylene blue (MB) for cannabis plants introduces compounds that primarily influence nitrogen metabolism, stress response, and potentially secondary metabolite production. These components are known for their roles in boosting nitric oxide production in animals, but their effects on plants, including cannabis, are less studied. Below, I’ll outline the potential effects of each component in the NO capsule on cannabis plants, their interactions with the existing mix, and the likely outcomes, drawing from plant science principles and inferred mechanisms due to limited cannabis-specific data. ### Effects of Nitric Oxide Capsule Components on Cannabis Plants 1. **L-Arginine (200 mg)**: - **Role in Plants**: L-Arginine is a precursor to nitric oxide (NO) in plants via the nitric oxide synthase-like pathway. NO regulates growth, stress response, and secondary metabolism. In cannabis, arginine (50–500 mg/L) may enhance root development, flowering, and stress tolerance by modulating NO signaling. - **Stress Response**: NO from arginine mitigates oxidative stress from drought, salinity, or heavy metals, potentially protecting cannabis in harsh environments. - **Secondary Metabolites**: NO influences terpene and alkaloid synthesis, possibly increasing cannabinoid (e.g., THC, CBD) or terpene production in cannabis buds, though direct evidence is limited. 2. **Arginine AKG 2:1 Extract (200 mg)**: - **Role in Plants**: Arginine alpha-ketoglutarate combines arginine with alpha-ketoglutarate, a TCA cycle intermediate. In plants, it may enhance nitrogen assimilation and energy metabolism. Alpha-ketoglutarate could support carbon metabolism, boosting photosynthesis and growth in cannabis. - **Synergy**: Provides additional arginine for NO production and supports metabolic pathways, potentially amplifying arginine’s effects. 3. **L-Citrulline HCl (200 mg) and L-Citrulline Malate Extract (100 mg)**: - **Role in Plants**: L-Citrulline is a precursor to arginine in the urea cycle, indirectly boosting NO production. In plants, citrulline (50–500 mg/L) accumulates under stress (e.g., drought), acting as a nitrogen storage compound and osmoprotectant. In cannabis, it may enhance stress tolerance and nitrogen use efficiency. - **Malate Contribution**: Citrulline malate provides malic acid, which supports the TCA cycle and photosynthesis, potentially increasing cannabis biomass and energy for growth. - **Cannabis-Specific**: Citrulline may improve root and shoot growth in hydroponic systems by enhancing nitrogen metabolism. 4. **Beta-Alanine (40 mg)**: - **Role in Plants**: Beta-alanine is a non-protein amino acid and precursor to pantothenate (vitamin B5) and coenzyme A, which are critical for lipid and secondary metabolite synthesis. In plants, it may enhance stress tolerance by supporting metabolic pathways. Its role in cannabis is less studied but could indirectly support terpene or cannabinoid production via coenzyme A. - **Limited Impact**: At 40 mg, the dose is low, so effects may be minimal compared to other components. **Application Notes**: The NO capsule components (total 740 mg) are typically dissolved for human consumption, but for cannabis, they’d be diluted in water (e.g., 740 mg in 1–10 L, yielding ~74–740 mg/L). A lower concentration (e.g., 100–500 mg/L) is safer for foliar or root application to avoid nitrogen overload. ### Synergistic Effects with EAAs, Proline, TMG, Glutamine, L-Glycine, and Methylene Blue The NO capsule components may enhance the existing mix’s effects on cannabis plants: 1. **Growth and Biomass**: - **Arginine/Citrulline + EAAs/Glutamine/Glycine**: Arginine and citrulline boost NO production and nitrogen metabolism, complementing EAAs, glutamine, and glycine’s roles in protein synthesis and nutrient uptake. This could enhance cannabis vegetative growth, root development, and bud size. - **Alpha-Ketoglutarate + Glutamine**: Alpha-ketoglutarate supports the TCA cycle, amplifying glutamine’s role in nitrogen assimilation, potentially increasing photosynthetic efficiency and biomass. - **Beta-Alanine**: May support lipid metabolism, contributing to cell membrane formation and bud quality, though its low dose limits impact. 2. **Stress Resilience**: - **Arginine/Citrulline + Proline/TMG/MB**: Arginine and citrulline’s NO production enhances stress signaling, complementing proline and TMG’s osmoprotectant roles and MB’s antioxidant effects. Glycine’s glutathione production further bolsters stress tolerance. This mix could significantly improve cannabis resilience to drought, heat, or high light, reducing leaf wilting or nutrient stress. - **Cannabis-Specific**: Ideal for outdoor grows or high-intensity indoor setups where environmental stressors are common. 3. **Secondary Metabolite Production**: - **Arginine/Citrulline + Glutamine/TMG/Glycine**: NO from arginine and citrulline may upregulate pathways for cannabinoid and terpene synthesis, as NO influences phenylpropanoid and alkaloid pathways in plants. Glutamine, glycine, and TMG support precursor synthesis (e.g., GABA, purines, methylation), potentially enhancing THC, CBD, or terpene yields. - **Beta-Alanine**: Supports coenzyme A production, which may aid terpene synthesis, though its low dose may limit impact. - **MB Synergy**: MB’s photosynthetic enhancement could provide energy for NO-driven secondary metabolism, amplifying cannabinoid production, though this is speculative. 4. **Nutrient Efficiency and Pathogen Resistance**: - **Arginine/Citrulline + Glycine/EAAs**: Arginine and citrulline improve nitrogen use efficiency, complementing glycine’s chelating properties and EAAs’ nutrient uptake enhancement, optimizing cannabis growth in hydroponics or nutrient-poor soils. - **MB Synergy**: MB’s antimicrobial properties may synergize with NO’s role in plant defense signaling, protecting cannabis from pathogens like *Botrytis* or *Pythium*. ### Potential Risks and Considerations - **Nitrogen Overload**: The NO capsule adds arginine, citrulline, and beta-alanine, all nitrogen-containing compounds, to an already nitrogen-rich mix (EAAs, glutamine, glycine). This increases the risk of nitrogen toxicity in cannabis, causing dark green leaves, clawing, or reduced flowering. Dilute to 100–500 mg/L total and monitor plant response. - **Absorption Competition**: Arginine, citrulline, EAAs, glutamine, and glycine may compete for root or foliar uptake, reducing efficacy. Stagger applications (e.g., NO capsule components in the morning, others in the evening) or alternate days. - **MB Interaction**: MB’s redox activity may interact with NO from arginine/citrulline, potentially amplifying or disrupting signaling if doses are unbalanced. Keep MB at ~1 mg/L (e.g., two drops of 1% solution per liter). - **Beta-Alanine Limited Impact**: At 40 mg, beta-alanine’s effects may be negligible, as plant studies use higher concentrations (e.g., 100–500 mg/L) for measurable benefits. - **Toxicity Risk**: High doses of arginine or citrulline (1000 mg/L) may disrupt nitrogen balance, while MB (3 mg/L) could cause photodynamic damage. Test low doses first. - **Cultivar Variability**: Sativa-dominant cannabis may benefit more from stress mitigation (arginine, citrulline, proline, TMG, MB), while indicas may respond better to growth promotion (EAAs, glutamine, glycine). ### Practical Recommendations - **Dosing**: - NO Capsule: Dissolve one capsule (740 mg total: 200 mg L-arginine, 200 mg arginine AKG, 200 mg L-citrulline HCl, 100 mg L-citrulline malate, 40 mg beta-alanine) in 2–10 L water (~74–370 mg/L). Start at 100–200 mg/L for foliar or root application. - EAAs: 150–300 mg/L - Glutamine: 250–500 mg/L - Proline: 50–100 mg/L - TMG: 0.5–1 g/L - Glycine: 100–500 mg/L - MB: ~1 mg/L (e.g., two drops of 1% solution per liter) - Apply twice weekly during vegetative growth or early flowering. - **Application**: - **Foliar Spray**: Apply during low-light periods (e.g., early morning) to avoid MB phototoxicity. Combine NO capsule components with amino acids for balanced uptake. - **Root Drench**: Suitable for hydroponics or soil, ensuring low MB concentrations to avoid microbial disruption. - **Staggering**: Apply NO capsule + MB separately from amino acids/TMG to minimize uptake competition (e.g., NO/MB in morning, others in evening). - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener leaves, robust growth). Test on a few plants first, especially for MB and NO components, due to limited cannabis data. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. - **Cultivation Context**: - **Hydroponics**: Use lower doses (~100 mg/L NO capsule, 0.5 mg/L MB) to avoid nutrient lockout or microbial imbalance. - **Soil**: Maintain healthy microbiota, as MB and high nitrogen inputs could disrupt beneficial microbes. - **Cultivars**: Adjust based on strain needs (e.g., stress-prone sativas vs. nutrient-hungry indicas). ### Evidence Gaps - **No Specific Studies**: No research addresses this exact combination (NO capsule + EAAs, proline, TMG, glutamine, glycine, MB) in cannabis. Effects are inferred from individual compound studies in plants (e.g., arginine for NO, MB in algae). - **Cannabinoid/Terpene Impact**: NO and MB may enhance secondary metabolism, but direct effects on THC/CBD or terpenes are unconfirmed. Cannabis-specific trials are needed. - **Beta-Alanine Role**: At 40 mg, beta-alanine’s impact is likely minimal, as plant studies use higher doses. - **Long-Term Effects**: Long-term impacts of MB and NO precursors on cannabis growth or soil health are unknown. Short-term use appears safe at low doses. ### Conclusion Adding a nitric oxide capsule (L-arginine, arginine AKG, L-citrulline, citrulline malate, beta-alanine) to EAAs, proline, TMG, glutamine, glycine, and MB likely enhances cannabis growth, stress tolerance, and nutrient efficiency by boosting NO signaling, nitrogen metabolism, and antioxidant defenses. The mix may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. However, nitrogen overload, absorption competition, and MB’s phototoxicity require careful dosing (e.g., 100–200 mg/L NO capsule, 1 mg/L MB). Test on a small scale, monitor closely, and consult a cannabis cultivation expert for optimization. If you provide the application volume (e.g., liters of water), specific strain, or growth stage, I can refine dosing further. Want me to search for studies on NO precursors in plants or focus on a specific aspect (e.g., NO’s role in cannabinoid synthesis)? 7/31 245am 🚨 Adding methylene blue (MB) to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, and L-glycine for cannabis plants introduces a compound with unique properties that may influence plant physiology, primarily through its roles as a redox agent, photosensitizer, and potential biostimulant. While methylene blue is more commonly studied in medical and aquatic contexts, its use in plant cultivation, including cannabis, is less documented, and specific studies on this exact combination are absent. Below, I’ll outline the potential effects of methylene blue on cannabis plants, its interactions with the other compounds, and the likely outcomes, drawing from available plant science knowledge and inferred mechanisms. Given the novelty of MB in this context, recommendations are cautious and speculative where direct evidence is lacking. Effects of Methylene Blue on Cannabis Plants Methylene blue (C16H18ClN3S) is a synthetic dye with redox, antimicrobial, and photosensitizing properties. Its effects on plants depend on concentration, application method, and environmental conditions: * Antioxidant and Redox Activity: At low concentrations (e.g., 0.1–10 µM or ~0.03–3 mg/L), MB acts as a redox mediator, scavenging reactive oxygen species (ROS) and protecting cells from oxidative stress caused by drought, high light, or salinity. In cannabis, this could enhance stress tolerance, particularly in high-intensity indoor grows or outdoor environments with temperature fluctuations. * Photosynthesis Enhancement: MB can act as an electron carrier in photosynthetic pathways, potentially improving electron transport in photosystem II. Studies on algae and some plants suggest low doses (e.g., 1–5 µM) may boost chlorophyll fluorescence and photosynthetic efficiency, potentially increasing cannabis biomass and bud quality. * Antimicrobial Effects: MB has antifungal and antibacterial properties, which could protect cannabis roots or foliage from pathogens like Pythium or Botrytis (bud rot). This is particularly relevant in hydroponic systems or humid environments. * Stress Mitigation: MB may stabilize cellular membranes under stress, similar to proline and TMG, potentially reducing damage from environmental extremes. * Potential Toxicity at High Doses: At higher concentrations (10–50 µM or ~3–15 mg/L), MB can generate ROS via photodynamic action under light, causing oxidative damage to plant tissues. This makes precise dosing critical. Application Notes: Two drops of MB (assuming a standard 1% solution, ~10 mg/mL, where 1 drop ≈ 0.05 mL) in, e.g., 1 liter of water yields ~1 mg/L (3 µM), a low concentration likely safe for foliar or root application. However, exact dosing depends on solution strength and volume. Synergistic Effects with EAAs, Proline, TMG, Glutamine, and L-Glycine Adding MB to the existing mix may enhance or modulate the effects on cannabis plants: 1. Enhanced Stress Resilience: * MB + Proline/TMG/Glycine: Proline, TMG, and glycine act as osmoprotectants and antioxidants, while MB scavenges ROS and stabilizes redox balance. Together, they could significantly improve cannabis tolerance to drought, heat, or salinity, reducing leaf wilting or nutrient stress in challenging environments (e.g., outdoor grows or high-EC hydroponics). * Glutamine/EAAs Synergy: Glutamine and EAAs support glutathione production, which complements MB’s antioxidant effects, potentially amplifying protection against oxidative stress from high light or nutrient imbalances. 2. Growth and Biomass: * MB + EAAs/Glutamine/Glycine: EAAs, glutamine, and glycine drive protein synthesis and nitrogen assimilation, while MB’s potential to enhance photosynthesis could provide the energy needed for these processes. This may lead to increased vegetative growth, larger leaves, and potentially denser buds in cannabis. * Proline Contribution: Proline supports tissue integrity, complementing MB’s membrane-stabilizing effects, which could enhance overall plant vigor. 3. Secondary Metabolite Production: * MB + Glutamine/TMG/Glycine: MB’s role as a photosensitizer may influence metabolic pathways by altering redox states, potentially enhancing synthesis of cannabinoids (e.g., THC, CBD) or terpenes. Glutamine and glycine contribute to precursor pathways (e.g., GABA, purines), while TMG’s methyl donation supports enzyme activity. This combination could theoretically increase terpene diversity or cannabinoid yield, though direct evidence is lacking. * EAAs Role: Methionine (an EAA) and glycine may support sulfur-based terpenes, with MB potentially amplifying metabolic efficiency via improved photosynthesis. 4. Nutrient and Pathogen Management: * MB + Glycine/EAAs: Glycine’s chelating properties and EAAs’ nutrient uptake enhancement could work with MB’s antimicrobial effects to improve nutrient availability and protect roots from pathogens in hydroponic or soil systems. * TMG/Glutamine: TMG’s cellular hydration and glutamine’s role in nitrogen metabolism may enhance MB’s ability to maintain cellular function under stress, improving overall nutrient efficiency. Potential Risks and Considerations * Toxicity Risk: MB at concentrations 10 µM (~3 mg/L) can produce ROS under light, damaging chloroplasts or cell membranes. Two drops in 1 liter (~1 mg/L) is likely safe, but overuse or concentrated solutions could harm cannabis, causing leaf burn or stunted growth. * Absorption Competition: MB may interact with amino acids during uptake, potentially reducing the efficacy of EAAs, glutamine, or glycine if applied simultaneously. Stagger applications (e.g., MB foliar spray, amino acids via root drench) to minimize this. * Nitrogen Overload: Adding MB to an already nitrogen-rich mix (EAAs, glutamine, glycine) increases the risk of toxicity, especially in cannabis, which is sensitive to excess nitrogen. Monitor for dark green leaves, clawing, or reduced flowering. * Photodynamic Effects: MB’s photosensitizing properties require careful application timing. Apply during low-light periods (e.g., early morning or evening) to avoid ROS generation in high-intensity grow lights. * Limited Cannabis Data: MB’s effects are mostly studied in algae, bacteria, or medical contexts, not cannabis. Its benefits are speculative, and plant-specific responses may vary by cultivar (e.g., sativa vs. indica). Practical Recommendations * Dosing: * MB: Two drops of 1% MB solution (~1 mg/L or 3 µM) per liter of water for foliar spray or root drench. Start with 1 drop/L to test tolerance. * EAAs: 150–300 mg/L * Glutamine: 250–500 mg/L * Proline: 50–100 mg/L * TMG: 0.5–1 g/L * Glycine: 100–500 mg/L * Apply twice weekly during vegetative growth or early flowering, adjusting based on plant response. * Application: * Foliar Spray: Apply MB and amino acids in low-light conditions to avoid phototoxicity. Use a fine mist to cover leaves evenly. * Root Drench: Suitable for hydroponics or soil, ensuring MB is diluted to avoid root damage. * Stagger Applications: Apply MB separately from amino acids (e.g., MB in the morning, amino acids in the evening) to reduce uptake competition. * Monitoring: Check for signs of stress relief (greener leaves, faster growth) or toxicity (yellowing, wilting). Test on a few plants first, especially for MB, as cannabis sensitivity is unknown. * Synergy with Nutrients: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. * Cultivation Context: * Hydroponics: Use lower MB doses (~0.5 mg/L) to avoid nutrient lockout or microbial disruption in sterile systems. * Soil: Ensure healthy soil microbiota, as MB’s antimicrobial effects could impact beneficial microbes if overused. * Cultivars: Sativa-dominant strains may benefit more from MB’s stress mitigation, while indicas may respond better to amino acid-driven growth. Evidence Gaps * No Specific Studies: No research addresses MB combined with EAAs, proline, TMG, glutamine, and glycine in cannabis. MB’s effects are extrapolated from algae, bacteria, or limited plant studies (e.g., Arabidopsis, lettuce). * Cannabinoid/Terpene Impact: MB’s influence on secondary metabolites is speculative. Its redox and photosynthetic effects may enhance terpene or cannabinoid synthesis, but cannabis-specific data are needed. * Long-Term Effects: MB’s long-term impact on cannabis growth, soil microbiota, or bud quality is unknown. Short-term studies suggest low doses are safe, but chronic use requires caution. * X Search Results: No recent posts on X discuss MB in cannabis cultivation with this combination. General posts mention MB for water treatment or medical uses, not plant applications. Conclusion Adding two drops of methylene blue (~1 mg/L) to a mix of EAAs, proline, TMG, glutamine, and glycine likely enhances cannabis stress tolerance, photosynthetic efficiency, and pathogen resistance, complementing the growth-promoting and nutrient-uptake benefits of the amino acids and TMG. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) effects on cannabinoid and terpene production. However, MB’s photodynamic and antimicrobial properties require precise dosing and application timing to avoid toxicity or microbial disruption. Start with low doses, test on a small scale, and monitor closely, consulting a cannabis cultivation expert for optimization. If you provide the MB solution concentration, application volume, or specific cannabis strain, I can refine dosing recommendations. Want me to search again for MB-related plant studies or focus on a specific aspect (e.g., MB’s photosynthetic effects)?......./. 245am L-GLYCINE AND Mg-Zyme by Biotics Research... LIKE NON DRUG TRANQ...KNOCKED ME OUT FOR 2 HOURS..WOW. VERY NICE!!!! 130pm very light mixture of nutrients and amino‘s are having a nice effect. I put a little in a spray bottle and I sprayed them twice once at the beginning of cycle to see if there’d be any damage there was not, but there is pretty nice growth they look much better than I thought👍👍 8/1 1057pm 🚨 Daily Consumption of a Specially Formulated Essential Amino Acid-Based Dietary Supplement Improves Physical Performance in Older Adults With Low Physical Functioning. 🚨 https://pmc.ncbi.nlm.nih.gov/articles/PMC8202157/ 🚨. 8/2 222am. 🚨⚡️🚨🚨⚡️⚡️. 🚨⚡️🚨🚨⚡️⚡️. AMINO FOLAR SPRAY SUCCESS!!!! THESE AMINOS DO NOT STINK SO SPRAYING IS NOT A PROBLEM. ADVANCED NUTES. GROW AT 1ML PER L WITH AMINOS GAVE A NICE FOLAR SPRAY.....NO BURNS, NO WILTING, JUST HAPPY HAPPY PLANTS. ACROSS CONES AND SEEDLINGS!!!!! ALSO WORKED ON NEW ROMAIN LETTUS, CHIVES, 4 VARIETIES OF NEW BASIL, EUCINASIA, AND ORANGE FLOWER SOMETHING!!!!!!🚨⚡️🚨🚨⚡️⚡️🚨⚡️🚨🚨⚡️⚡️. .

CalGonJim 8/4 130am MONDAY. POOR RICKY....HIS HEAD FELL OFF ALL BY ITSELF....THATS MY STORY SO JUST SHUT UP....LETS NOT SPEAK OF IT AGAIN....... WE HAVE A NEW COOKIE APPLE IN A 2 GALLON NOW. TRIPLOID IS DOING FINE ALMOST INDISTINGUISHABLE FROM COOKIE APPLE NEXT TO IT. Monday 2:47 AM nutrient mix today: in a 2 L solution: one Nitric Boost capsule (nitric oxide (NO) capsule containing L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), and beta-alanine (40 mg), essential amino acids 300 mg, glutamine 500 mg, proline 100 mg, TMG powder 1 g, glycine 1 g, methylene blue 5% solution two drops, 3 mL each advanced nutrients connoisseur a and B in a 2 L solution solution pH balanced to 5.9. 💎🚨⚡️. Thank you for sharing the details of your nutrient mix for your cannabis grow. Your mix is quite complex, incorporating a variety of compounds, including nitric oxide (NO) precursors, amino acids, methylene blue, and Advanced Nutrients Connoisseur A and B, all in a 2 L solution pH-balanced to 5.9. Since you’re asking about this in the context of a cannabis grow and previously mentioned using essential amino acid powder and comparing FloraKleen vs. SledgeHammer, I’ll assume you’re seeking insight into how this nutrient mix might affect your cannabis plants, particularly in a hydroponic or soilless setup (given the pH of 5.9, which is typical for hydroponics or coco). I’ll evaluate the components, their potential effects on cannabis, interactions, and considerations for optimizing your grow, while addressing the inclusion of methylene blue and its relevance to plant health. ### Breakdown of Your Nutrient Mix Let’s analyze each component in your 2 L solution, its role in cannabis cultivation, and potential benefits or risks: #### 1. Nitric Boost Capsule - **Ingredients**: L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), beta-alanine (40 mg). - **Purpose in Humans**: These are nitric oxide (NO) precursors, used to increase NO production, which improves blood flow and muscle performance. In plants, NO plays a role in signaling, stress response, and growth regulation. - **Effects on Cannabis**: - **L-Arginine and L-Citrulline**: In plants, arginine is a key amino acid involved in nitrogen metabolism and polyamine synthesis, which supports cell division and stress resistance. Citrulline can contribute to arginine biosynthesis, potentially enhancing nitrogen availability. These may promote vegetative growth and root development, but excessive nitrogen from these compounds could delay flowering or reduce bud quality if not balanced. - **Arginine AKG**: AKG is a keto acid involved in the Krebs cycle, potentially boosting energy metabolism in plants. Limited research exists on its direct use in cannabis, but it may enhance nutrient uptake and photosynthesis. - **Beta-Alanine**: Less studied in plants, but it may act as a stress buffer, helping cannabis cope with environmental stressors (e.g., high light or temperature). Its low dose (40 mg) likely has minimal impact. - **Nitric Oxide Role**: NO in plants regulates stomatal closure, photosynthesis, and stress responses (e.g., drought, salinity). Low doses from these precursors could enhance resilience and growth, but high doses might disrupt hormonal balance (e.g., gibberellins, abscisic acid), potentially affecting flowering. - **Concentration**: Total 740 mg in 2 L = ~370 mg/L (0.37 g/L). This is a moderate dose, but the specific impact depends on how often you apply it and the growth stage. #### 2. Essential Amino Acids (300 mg) - **Purpose**: As discussed in your previous question, essential amino acids (e.g., lysine, methionine, threonine) reduce the energy plants spend on amino acid synthesis, supporting growth, terpene production, and stress resistance. - **Effects on Cannabis**: - Enhances protein synthesis, leading to stronger vegetative growth and potentially larger buds. - Increases terpene content (e.g., limonene, β-myrcene), improving aroma and flavor, but may reduce THCA (THC precursor) if overused, especially in late flowering. - Boosts nutrient uptake by chelating micronutrients, but can reduce calcium and iron levels, requiring monitoring. - **Concentration**: 300 mg in 2 L = 150 mg/L. This is a reasonable dose for hydroponics, aligning with grower recommendations (e.g., 1/8 tbsp per 12 gal ≈ 100–200 mg/L). #### 3. Glutamine (500 mg) - **Purpose**: Glutamine is a key amino acid for nitrogen transport and storage in plants, supporting chlorophyll synthesis and photosynthesis. - **Effects on Cannabis**: - Promotes lush vegetative growth and greener leaves, ideal for the vegetative phase. - Enhances stress tolerance by supporting glutathione production, which combats oxidative stress. - May contribute to nitrogen buildup, so use cautiously in late flowering to avoid affecting bud taste or potency. - **Concentration**: 500 mg in 2 L = 250 mg/L. This is a moderate-to-high dose, effective for vegetative growth but potentially excessive in late flowering. #### 4. Proline (100 mg) - **Purpose**: Proline is an amino acid that accumulates in plants under stress (e.g., drought, salinity), acting as an osmoprotectant and antioxidant. - **Effects on Cannabis**: - Improves stress resistance, particularly under high light, heat, or nutrient stress, common in indoor cannabis grows. - Supports pollen development and flowering, potentially increasing bud set. - Synergizes with other amino acids to enhance overall plant vigor. - **Concentration**: 100 mg in 2 L = 50 mg/L. This is a low, safe dose, effective for stress mitigation without risking nutrient imbalance. #### 5. TMG (Trimethylglycine/Betaine, 1 g) - **Purpose**: TMG is an osmoprotectant and methyl donor, used in plants to enhance stress tolerance and metabolic processes. - **Effects on Cannabis**: - Protects against environmental stress (e.g., high EC, temperature swings), improving plant resilience in hydroponic systems. - May enhance photosynthesis and nutrient uptake by stabilizing proteins and membranes. - Limited direct evidence for cannabis, but it’s used in agriculture to boost yield under stress. - **Concentration**: 1 g in 2 L = 500 mg/L. This is a high dose, potentially beneficial for stressed plants but unnecessary in optimal conditions, risking nutrient burn if overused. #### 6. Glycine (1 g) - **Purpose**: Glycine is an amino acid involved in chlorophyll synthesis, protein production, and chelation of micronutrients. - **Effects on Cannabis**: - Boosts photosynthesis, leading to greener, healthier plants. - Enhances nutrient uptake by chelating metals like zinc and iron, though your mix may already reduce iron/calcium, so monitor for deficiencies. - Supports terpene and cannabinoid synthesis indirectly via metabolic efficiency. - **Concentration**: 1 g in 2 L = 500 mg/L. This is a high dose, effective for vegetative growth but potentially excessive in flowering, as it may contribute to nitrogen buildup. #### 7. Methylene Blue (5% Solution, 2 Drops) - **Purpose**: Methylene blue is not a standard plant nutrient but has been explored in agriculture for its redox properties and potential antimicrobial effects. - **Effects on Cannabis**: - **Mitochondrial Enhancement**: In plants, methylene blue may act as an electron carrier, potentially improving mitochondrial efficiency and energy production, similar to its effects in humans (as you noted feeling great after taking it). This could enhance growth and stress tolerance. - **Antioxidant Properties**: At low doses, it may reduce oxidative stress, protecting cannabis from high light or nutrient stress. - **Antimicrobial Potential**: May suppress fungal or bacterial growth in the root zone, beneficial in hydroponics where pathogens can proliferate. - **Risks**: No established use in cannabis cultivation, and high doses could be phytotoxic or disrupt microbial balance in soil. The 5% solution (50 mg/mL) at 2 drops (~0.1 mL) = ~5 mg in 2 L = 2.5 mg/L. This is a very low dose, likely safe but with unproven benefits for plants. - **Note**: Its inclusion is experimental, and there’s limited research on methylene blue in cannabis. Your positive personal experience with it might suggest a hypothesis that it benefits plant metabolism similarly, but caution is warranted. #### 8. Advanced Nutrients Connoisseur A and B (3 mL each) - **Purpose**: Connoisseur A and B is a premium 2-part nutrient system designed for cannabis, providing macro- and micronutrients, with a focus on bloom enhancement. - **Effects on Cannabis**: - Supplies balanced NPK (nitrogen, phosphorus, potassium) and micronutrients (e.g., calcium, magnesium, iron) tailored for cannabis, promoting vigorous vegetative growth and heavy flowering. - Contains proprietary additives (e.g., humates, wetting agents) to enhance nutrient uptake and bud development. - High phosphorus and potassium in Part B support dense, resinous buds and terpene production. - **Concentration**: 3 mL each in 2 L = 1.5 mL/L (total 3 mL/L). This is within Advanced Nutrients’ recommended range (2–4 mL/L for bloom phase), suitable for flowering cannabis in hydroponics. #### 9. pH (5.9) - **Suitability**: A pH of 5.9 is ideal for hydroponic or coco cannabis grows, as it optimizes nutrient availability (most nutrients are bioavailable at pH 5.5–6.2). This suggests your setup is hydroponic or coco-based, aligning with the use of Advanced Nutrients and amino acids. ### Overall Effects on Cannabis - **Vegetative Phase**: - The mix is nitrogen-heavy (from L-arginine, glutamine, glycine, amino acids, and Connoisseur A), promoting lush vegetative growth, strong stems, and green leaves. Glutamine and glycine boost chlorophyll synthesis, while proline and TMG enhance stress resistance, ideal for early growth under high light or CO₂. - Nitric oxide precursors (L-arginine, L-citrulline) may enhance root development and nutrient uptake, supporting vigorous plants. - Methylene blue’s potential mitochondrial and antimicrobial effects could improve plant vigor, but its impact is speculative. - **Flowering Phase**: - Connoisseur A and B provide high phosphorus and potassium, essential for bud development and resin production. Amino acids (especially glycine, proline) may boost terpene content, enhancing aroma and flavor. - However, the high nitrogen content (from amino acids, glutamine, glycine, L-arginine) could delay flowering or reduce bud quality if used late in the cycle, as excess nitrogen can lead to leafy buds and lower THC. - Previous research (from your amino acid question) suggests amino acids may reduce THCA, so monitor potency if this is a priority. - Methylene blue’s role in flowering is unclear; it may help with stress but could disrupt late-stage metabolism if misapplied. - **Stress Resistance**: - Proline, TMG, and beta-alanine enhance resilience to environmental stressors (e.g., high EC, heat, light intensity), which is valuable in hydroponics. - Methylene blue’s antioxidant properties might complement this, but its efficacy is unproven. ### Potential Interactions and Risks 1. **Nitrogen Overload**: - The mix contains multiple nitrogen sources (L-arginine, glutamine, glycine, essential amino acids, Connoisseur A). This could lead to excessive vegetative growth, delaying flowering or causing nutrient burn (e.g., clawing leaves, dark green foliage). - **Solution**: Reduce amino acid doses (e.g., cut glutamine or glycine to 250 mg each) in late vegetative or early flowering to balance nitrogen. 2. **Nutrient Imbalances**: - Amino acids can reduce calcium and iron uptake (as noted in your previous question). Connoisseur A and B provide these micronutrients, but monitor for deficiencies (e.g., yellowing leaves, interveinal chlorosis). - **Solution**: Consider adding a calmag supplement (e.g., 1 mL/L CaliMagic) if signs of deficiency appear, especially in coco or hydroponics. 3. **Methylene Blue Risks**: - At 2.5 mg/L, methylene blue is unlikely to be phytotoxic, but its effects on cannabis are unstudied. It could disrupt beneficial microbes in the root zone or alter nutrient uptake if overused. - **Solution**: Use methylene blue sparingly (e.g., once weekly) and monitor plant response. Discontinue if you notice stunted growth or discoloration. 4. **High TMG and Glycine Doses**: - At 500 mg/L each, TMG and glycine are high compared to typical plant applications (100–300 mg/L). While beneficial for stress, they may contribute to nutrient buildup or EC spikes. - **Solution**: Reduce to 250–300 mg/L and test EC (aim for 1.2–2.0 mS/cm in bloom). 5. **EC and pH Stability**: - The mix’s complexity (multiple amino acids, NO precursors, nutrients) could raise EC, risking nutrient burn. The pH of 5.9 is good, but frequent monitoring is needed as amino acids and acids in Connoisseur can lower pH over time. - **Solution**: Measure EC (should be ~1.0–1.5 mS/cm in veg, 1.5–2.2 mS/cm in bloom) and adjust nutrient strength if needed. ### Recommendations for Optimization 1. **Adjust for Growth Stage**: - **Vegetative Phase**: Your mix is well-suited for vegetative growth due to high nitrogen and stress-protective compounds. Continue as is, but monitor EC to avoid overfeeding. - **Flowering Phase**: Reduce nitrogen-heavy components (glutamine, glycine, essential amino acids) to 50% (e.g., 150 mg amino acids, 250 mg glutamine/glycine) starting week 3 of flowering to focus on bud development and prevent nitrogen buildup. Increase Connoisseur A and B to 4 mL/L in mid-bloom for higher PK. - **Final Flush**: Use FloraKleen (recommended from your previous question) at 5–10 mL/gal for the first 2–3 days of a 10-day flush, followed by RO water, to clear excess nitrogen and salts from amino acids and NO precursors. Stop methylene blue during flush to avoid residues. 2. **Monitor Nutrient Levels**: - Check for calcium/iron deficiencies (e.g., yellowing, spotting) due to amino acid chelation. Add a calmag supplement if needed. - Use a TDS meter to keep EC in check (1.2–2.2 mS/cm depending on stage). 3. **Methylene Blue Caution**: - Given its experimental nature, limit methylene blue to 1–2 drops per 2 L weekly and observe plant response. If no clear benefits (e.g., faster growth, greener leaves) are seen after 2–3 weeks, consider discontinuing to simplify the mix. - If you’re including it based on your positive personal experience, its low dose is unlikely to harm plants, but benefits are speculative. 4. **Flushing Strategy**: - Given your complex mix, periodic flushing (every 3–4 weeks) with FloraKleen (5 mL/gal) is recommended to prevent salt buildup, especially in hydroponics. This aligns with your previous interest in FloraKleen vs. SledgeHammer, where FloraKleen was preferred for hydroponic cannabis. 5. **Terpene and Cannabinoid Focus**: - Your amino acid-heavy mix may boost terpenes (e.g., limonene, β-myrcene), enhancing bud aroma, but could reduce THCA. To maximize THC, reduce amino acids in weeks 5–8 of flowering and rely on Connoisseur B’s high PK. - Consider adding a bloom booster (e.g., Advanced Nutrients Bud Candy, 2 mL/L) to enhance terpenes and sugars without nitrogen overload. ### Addressing Methylene Blue Your inclusion of methylene blue is intriguing, likely inspired by its benefits for you (energy, mood) and its potential to enhance mitochondrial function or reduce oxidative stress. In plants, its redox properties might theoretically improve energy metabolism or protect against stress, but there’s no direct evidence for cannabis. The low dose (2.5 mg/L) minimizes risks, but I’d recommend treating it as an experiment: - **Test**: Apply to a single plant or reservoir for 2 weeks and compare growth, leaf color, and bud development to untreated plants. - **Discontinue if No Benefit**: If no improvement is seen, simplify your mix to avoid potential residues or interactions. ### Conclusion Your nutrient mix is robust for vegetative growth, with amino acids (glutamine, glycine, proline, essential amino acids), NO precursors, and Connoisseur A and B promoting vigorous plants and stress resistance. However, the high nitrogen content and experimental methylene blue require careful management to avoid delays in flowering or reduced THC potency. For optimal results: - Reduce nitrogen-heavy components (glutamine, glycine, amino acids) in flowering to focus on bud development. - Use FloraKleen for periodic and final flushes to clear excess salts, especially given the amino acid and NO precursor load. - Monitor EC, pH, and micronutrients (calcium, iron) to prevent imbalances. - Treat methylene blue as an experiment and discontinue if no clear benefits are observed. If you can share more details (e.g., growth stage, hydroponic vs. coco, strain type, or specific goals like THC vs. terpenes), I can refine the recommendations further. I can also search X or recent studies for grower experiences with similar mixes or provide a tailored nutrient schedule. Let me know how you’d like to proceed! 👍. 8/7 214pm...!!!! wont update this one anymore until harvest!!! all are in the 🍓🍰🍌🍆🥊🤨🌿🍑🍪🍎 grow now!!!!!

CalGonJim 8/11, 1:15 AM Monday she’s in with the other diary but she’s doing good topped last week. She responded well. 8/14 1152AM She's doing ok, putting some backup notes here incase I have a moment...and delete something somewhere else (W/out MB) What are the combined effects of EAA, proline, Setria Glutathione, L glutamine, TMG powder, L glycine on Autoflower Cannabis ? ### Overview of the Stack for Autoflower Cannabis Autoflowering cannabis (autoflowers) are compact, fast-maturing strains that transition to flowering based on age, typically completing their cycle in 8-12 weeks. They are sensitive to nutrient overload, stress, and environmental factors, making biostimulants like Essential Amino Acids (EAA), proline, Setria Glutathione (a stable reduced glutathione form), L-glutamine, TMG (trimethylglycine, aka glycine betaine), and L-glycine potentially beneficial for enhancing vigor, stress tolerance, and yield. These compounds, derived from human nutrition but applicable to plant horticulture, act as osmoprotectants, antioxidants, and metabolic enhancers. No direct studies exist on this exact combination for autoflowers, but evidence from cannabis research, plant stress physiology, and grower extrapolations indicates synergistic effects on growth, resilience, and cannabinoid/terpene production. Benefits are drawn from hydroponic trials on medical cannabis and general plant studies, with applications via foliar sprays (0.1-1 g/L) or root drenches in veg/early flower stages. Autoflowers may respond particularly well due to their ruderalis genetics, which favor stress-resistant biostimulants without prolonging the cycle. #### Individual Effects on Autoflower Cannabis Based on scientific literature and inferred from cannabis/plant trials, here's a summary of each component's role: | Supplement | Key Effects on Autoflowers | Supporting Evidence | |------------|----------------------------|---------------------| | **EAA (Essential Amino Acids)** | Boosts protein synthesis, root/shoot growth, nutrient uptake; enhances cannabinoid/terpene standardization; improves stress resistance. | Amino acids increase secondary metabolites like THCA and monoterpenes (limonene up 81-123%, β-myrcene up 139-167%) in hydroponic cannabis; reduce CBNA by 44%. | | **Proline** | Osmoprotectant; enhances drought/salinity tolerance; reduces ROS; supports enzyme stability and cellular hydration. | Proline mitigates abiotic stresses (drought, salinity, heat, heavy metals) by boosting antioxidants and osmoprotection; coordinates with GSH for ROS scavenging. | | **Setria Glutathione** | Antioxidant; scavenges ROS; improves photosynthesis efficiency; enhances pest/disease resistance and metal detoxification. | GSH protects against oxidative damage from stresses like salinity/metal; maintains redox balance; no direct cannabis data, but applicable to autoflowers for yield protection. | | **L-Glutamine** | Nitrogen source; promotes chlorophyll, root growth; aids recovery from deficiencies/transplant shock; supports microbial activity. | Related to glutamic acid; enhances N uptake and growth in cannabis; inferred stress mitigation via antioxidant pathways. | | **TMG (Trimethylglycine)** | Osmoprotectant and methyl donor; improves heat/drought tolerance; enhances membrane stability and antioxidant enzymes. | Glycine betaine reduces ROS/lipid peroxidation; boosts photosynthesis and yield under stresses; no cannabis-specific, but broad plant benefits. | | **L-Glycine** | Supports protein/GSH synthesis; detoxifies heavy metals; promotes tight junctions for root health; reduces toxicity in contaminated soils. | Forms metal-glycine complexes to lower heavy metal uptake in cannabis; supports GSH for anti-aging/stress effects; inferred gut-like barrier protection in roots. | #### Combined Effects This stack could provide amplified benefits through synergies in nitrogen metabolism, antioxidant defense, and osmotic regulation, particularly suited to autoflowers' short cycle and stress sensitivity: - **Growth and Yield Enhancement**: EAAs, glutamine, and glycine increase N/S uptake and biomass (up to sharper flower growth from week 6 in trials), potentially boosting bud density and yield by 15-50% via protein synthesis and monoterpene elevation (e.g., limonene/β-myrcene for aroma/resin). Proline/TMG add osmotic support for robust stems/roots, reducing flop or abortion in dense colas. - **Stress Resistance**: Proline + glutathione coordinate to scavenge ROS, maintain redox balance, and protect against joint stresses (e.g., salinity + metals), decreasing H2O2 and enzyme damage. TMG/glycine enhance tolerance to drought/heat/heavy metals, with glycine detoxifying soil contaminants common in cannabis grows. Overall, reduced transplant shock, pest vulnerability, and environmental stunting. - **Secondary Metabolite Boost**: Amino acids standardize cannabinoids (higher THCA in recirculation setups) and terpenes; glutathione/proline reduce oxidative stress for better resin quality. Glycine + glutathione synergy supports longevity-like effects, potentially elevating THC/CBD by preserving mitochondrial function. - **Soil and Microbial Health**: Glutamine/glycine foster beneficial microbes (e.g., with myco powders for no shock); TMG aids nutrient cycling. In coco/hydro (ideal for autoflowers), prevents deficiencies and improves pH/EC stability. - **Overall Vigor**: Faster veg recovery, sustained flower development without extension; inferred 200g+ yields/plant under LEDs/hydro with reduced bud rot/foxtailing risks. In hydro trials, recirculation cycles with aminos yield higher metabolites than drain-to-waste, suggesting sustained exposure maximizes synergies. #### Potential Side Effects and Interactions - **Overload Risks**: High aminos (e.g., EAAs/glutamine) may cause N excess, leading to burn, stunting, or earlier maturation with lower yields in drain-to-waste setups. Proline/TMG could imbalance osmolytes if over-applied. - **Interactions**: Glutathione + proline synergize positively for ROS control, but excess glycine might strain metal detox in contaminated soils. Monitor pH/EC (5.8-6.2, EC 1.2-2.0) to avoid lockout. - **Autoflower-Specific**: Avoid late flower to prevent rot; high doses risk vigor depletion or fox-tailing. Test low (100-500 mg/L) weekly in veg. #### Recommendations Apply as dilute foliar/root feed in veg; pair with mycorrhizae for synergy. Coco outperforms soil for autoflowers. Results vary by strain/environment; consult grow communities. This is not agronomic advice. 🚨🚨🚨🚨⚡️⚡️🚨 I THINK I GOT IT!!!!!!! 8/15 445AM. ### Analysis of Your Nutrient and Amino Acid Mix for Autoflower Cannabis 🚨⚡️⚡️🚨 You're using a 2-liter solution with Advanced Nutrients pH Perfect Connoisseur Grow A & B at 2 ml/L each (total 4 ml/L, half the manufacturer's full strength of 4 ml/L each) combined with a biostimulant stack of 250 mg Setria L-Glutathione, 240 mg proline, 230 mg L-glutamine, 200 mg Essential Amino Acids (EAA), and 200 mg TMG (trimethylglycine) for autoflower cannabis. This mix is applied during the vegetative phase (weeks 2-4 typically for autos), leveraging Connoisseur's balanced NPK and micronutrients with amino acids and biostimulants to enhance growth, stress tolerance, and secondary metabolite production. Below, I evaluate the mix's efficacy and dosing based on cannabis research, plant physiology, and grower insights, drawing on your interest in nutrient optimization for autoflowers. #### Components and Their Roles 1. **Advanced Nutrients Connoisseur Grow A & B (2 ml/L each, 4 ml/L total)**: - **Purpose**: Provides macro (N, P, K), secondary (Ca, Mg), and chelated micronutrients (Fe, Mn, Zn) for vegetative growth. At half strength, it delivers moderate nutrition (EC ~1.0-1.4 mS/cm, depending on water), ideal for young or sensitive autoflowers to avoid burn while promoting leaf, stem, and root development. - **Effects**: Supports bushy growth, chlorophyll production, and root vigor. The pH Perfect technology stabilizes pH (5.5-6.5), reducing lockout and deficiencies (e.g., no yellowing from Fe/Mn issues). Growers report 20-30% biomass increase in veg at this dose, setting up autos for denser buds later. - **Suitability**: Half strength is conservative and well-suited for autoflowers in coco or hydro, especially under moderate lighting (e.g., LEDs at 300-600 PPFD), preventing overfeeding while maintaining vigor. 2. **Biostimulant Stack** (per 2 L solution): - **Setria L-Glutathione (250 mg, 125 mg/L)**: - **Role**: Master antioxidant; scavenges reactive oxygen species (ROS), enhances photosynthesis efficiency, and detoxifies heavy metals. Supports stress resistance (e.g., heat, light stress) and yield protection. - **Dose Evaluation**: 125 mg/L is within the safe range (100-300 mg/L from plant studies). It aligns with trials showing improved maize growth under stress at similar concentrations. Effective for autos without risking overload. - **Proline (240 mg, 120 mg/L)**: - **Role**: Osmoprotectant; mitigates drought, salinity, and oxidative stress by stabilizing enzymes and maintaining cellular hydration. Synergizes with glutathione for ROS scavenging. - **Dose Evaluation**: 120 mg/L (~1 mM) is appropriate, as plant studies suggest 100-300 mg/L (0.87-2.6 mM) for stress mitigation. Cannabis analogs confirm efficacy at this level for recovery and resilience. - **L-Glutamine (230 mg, 115 mg/L)**: - **Role**: Nitrogen donor; promotes chlorophyll synthesis, root growth, and microbial activity. Enhances N uptake and recovery from transplant shock or deficiencies. - **Dose Evaluation**: 115 mg/L is conservative and safe (within 100-400 mg/L from biostimulant trials). Supports veg growth without excessive N, complementing Connoisseur’s N supply. - **EAA (200 mg, 100 mg/L)**: - **Role**: Boosts protein synthesis, nutrient uptake, and secondary metabolites (e.g., limonene up 81-123%, β-myrcene up 139-167%). Standardizes cannabinoids (THCA/CBDA) but may reduce potency if overused. - **Dose Evaluation**: 100 mg/L is low but effective (trials suggest 150-500 mg/L). Ideal for autos to avoid N overload, especially with Connoisseur’s N content. - **TMG (Trimethylglycine, 200 mg, 100 mg/L)**: - **Role**: Methyl donor and osmoprotectant; enhances heat/drought tolerance, membrane stability, and terpene gene expression. Supports photosynthesis and yield. - **Dose Evaluation**: 100 mg/L (~0.85 mM) is slightly below typical plant study ranges (200-500 mg/L, 1.7-4.3 mM) but safe for autos, reducing risk of osmotic imbalance. #### Combined Effects This mix synergizes well for autoflowers in veg (weeks 2-4), leveraging Connoisseur’s stable nutrient base with biostimulants to enhance growth and resilience: - **Vegetative Growth and Vigor**: Connoisseur provides balanced NPK (moderate N at 2 ml/L) for leaf/stem development, while EAA, glutamine, and proline boost protein synthesis and root growth. Expect bushier plants with stronger internodes and 20-40% biomass increase, ideal for autos’ short veg phase. - **Stress Resistance**: Glutathione and proline scavenge ROS, protecting against light/heat stress, while TMG enhances osmotic balance for drought/salinity tolerance. This reduces stunting or shock, critical for autos’ sensitivity. - **Secondary Metabolite Boost**: EAA and TMG upregulate terpenes (e.g., myrcene, limonene) and standardize cannabinoids, potentially improving aroma and resin quality. Glutathione supports yield by reducing oxidative damage. - **Nutrient Uptake and Microbial Health**: Glutamine and TMG foster beneficial microbes, enhancing Connoisseur’s chelated nutrient absorption (e.g., Ca, Fe). Coco/hydro setups benefit most, with yields potentially reaching 150-250 g/plant under optimal conditions. #### Dose Evaluation - **Total Biostimulant Load**: 985 mg in 2 L = ~492.5 mg/L total (125 mg/L glutathione, 120 mg/L proline, 115 mg/L glutamine, 100 mg/L EAA, 100 mg/L TMG). This is within safe limits (