Irie Maiden, Romulan Genetics. HPA

Dudemann Back story- had a mishap with my original clones. Tried clone collars for the first time and failed. Unfortunately trimmed the crap out of my mothers so I had to take small cuts for this run.. either way they’re rooted and on the up. Week 1 load out directly below scroll past and I have the whole set up listed out. LOAD-OUT — VEG WEEK 1 (Oct 5 - Oct 11, 2025) AERIAL ENVIRONMENT (IONFRAME EVO3, AC Infinity 69 Pro) Photoperiod: 18/6 DLI/PPFD ramp: - Sun-Mon: DLI 10-11 (PPFD ~155-170) - Tue-Wed: DLI 12-13 (PPFD ~185-200) - Thu-Sat: up to DLI 14-16 (PPFD ~215-245) only if leaves are praying and color improving Temps: 74-79 F lights on; 70-74 F lights off RH/VPD: start 65-70% (VPD 0.9-1.05 kPa), drift to 60-65% by week end (VPD 1.0-1.15 kPa) Airflow: gentle oscillation; avoid direct breeze on 001 UVA bars: OFF this week CO2: ambient Balance note: give plant 001 about +10-15 umol more center-beam than 003 ROOT-CHAMBER ENVIRONMENT Pulse: 2.0 s ON / 5:00 OFF Droplet spectrum: Dv50 ~80-90 um Root zone temp (RZT): 65-67 F pH: 5.7-5.9 ORP: 200-300 mV (microbe-friendly) RESERVOIR (per 5 gal) Source: dehumidifier condensate Step 1 - Sterilize: add 25-30 mL plain 6% Clorox per 5 gal; keep lid on 3 days Step 2 - Dechlorinate and polish: Camco TastePURE RV (GAC+KDF) - WaterSentinel SF-20W DI Goal EC (fresh): 0.50-0.55 mS/cm DOSING (per 5 gal) Armor Si: 1.0 mL Calimagic: 3.0 mL Flora Micro: 5.0 mL Flora Bloom: 5.0 mL Flora Grow: 1.0 mL Floralicious Plus: 0.5-0.75 mL RapidStart: 3.0 mL SLF-100: 2.0 mL Microbe rotation: - Sun (fresh res): Tribus 1.0 mL (~1/4 dose) - Wed/Thu (top-off or mini-refresh): King Crab 0.5-1.0 mL HARDWARE (REFERENCE) High pressure misting specifics: Each 0.2 mm nozzle @ 140 psi ≈ 0.051 L/min → ~1.7 mL per 2 s pulse. All 16 nozzles = ~27 mL delivered per pulse → ~308 mL per hour total system output. Accumulator keeps pressure drop 5 psi on valve open — zero after-drip. Droplet Spectrum: Dv50: ~80–90 µm (ideal HPA range) Dv10–Dv90: ~40–140 µm spread Fine enough for high O₂ exchange, dense enough to stay wet without drip. Tent and structure - CLOUDLAB 642 (4x2 ft, 72 in) - 40 gal HDX tote (root chamber) with sealed lid Lighting - IONFRAME EVO3, 280 W (2x4 ft) - IONBEAM U2 UV LED bars, 2-bar kit, 11 in Air movement and environmental - AC Infinity Controller 69 Pro - Air Filtration Kit PRO 4 in (inline fan + carbon filter + ducting) - CLOUDRAY S6 Gen 2 clip fan, 6 in - AC Infinity CLOUDFORGE T3 humidifier - Portable air conditioner (room/tent) - Water chiller (reservoir) High-pressure aeroponics (core) - Aquatec 8800 booster pump - 5 gal pressure accumulator tank - Beduan 12 V normally-closed RO solenoid valve - BN-LINK short-period repeat cycle timer - 16x hydraulic mist nozzles, 0.2 mm orifice - 10x on dual 1/4 in concentric closed-ring manifold (bottom ring) - 6x top-mounted array (upper chamber) - 1/4 in tubing (manifold + feeds; dual concentric loops) Reservoir and fluid handling - 5 gal side reservoir (chilled) - Airstone bubbler (reservoir aeration) - Buckets for source-water prep and transfers - Inline filtration (post-sterilization): Camco TastePURE RV - WaterSentinel SF-20W DI Source-water sanitation - Clorox unscented 6% sodium hypochlorite (bleach) Instruments and meters - pH meter/pen-Apera Instruments AI311 Premium Series - EC/TDS meter - Tent thermo-hygrometer (for VPD) - Photone PAR meter app - GIDIGI 8-in-1 handheld water sensor(for resevior) Media - Oasis Rootcubes #5015 sheets(clone)

Dudemann WEEK 1 SUMMARY (Sept 28 – Oct 4 • Veg Week 1) Sprayers- 2 s ON / 5:15 OFF EC 0.29 → 0.55 mS/cm • pH 5.8 • RZT 65–67 °F Lighting: DLI 10 → 12 • 18/6 • RH 65–70 % • VPD ~1.0 kPa Source Water: sterilized dehu water. 001 (Compact pheno) • Root tips just past basket; first full leaf set by week’s end. • Stem two-tone (red upper / green-white lower). • Canopy compact, internodes tight. 003 (Vigorous pheno) • Roots reached chamber floor → curtain beginning. • Fuller canopy, steady transpiration, good leaf turgor. Incident Aero pump accidentally unplugged for several hours → partial root desiccation. Recovery achieved within 24 h (no canopy stress). End-of-Week Status: both clones stable, white new roots visible, foliage bright green, system primed for nutrient ramp. 001 is about a week behind, this tracks with the mother plant. The mother is slower growing and more compact. IRIE MAIDEN — WEEK 2 VEG LOAD-OUT Reservoir Formula (5 gal / 18.9 L) Target EC 0.60 – 0.65 mS/cm( Measured 0.612 mS/cm) pH 5.8 Armor Si 1.0 mL Calimagic 3.5 mL Flora Micro 10 mL Flora Bloom 10 mL Flora Grow 1.0 mL Floralicious Plus 0.5 mL Purpinator 5 mL Rapid Start 3.0 mL SLF-100 2.0 mL Microbial Rotation Day 1 – Tribus 1.0 mL (¼ dose) Day 4 – King Crab 0.5 – 1.0 mL Day 6-7 – Orca 0.5 mL ⸻ Environmental Load-Out Aerial Environment Photoperiod 18 / 6 Temp 77 – 80 °F (on) 70 – 72 °F (off) RH 60 – 65 % VPD 1.0 – 1.15 kPa DLI 13 → 15 (≈ 200 → 230 µmol PPFD) UVA OFF (this week) Light bias +10–15 µmol on 001 Airflow gentle oscillation Root-Chamber Environment Pulse 2.0 s ON / 5 min 15 s OFF RZT 65 – 67 °F pH 5.7 – 5.9 (target 5.8) ⸻ Notes • Source water EC 0.002 mS/cm

Dudemann Plant 003 (vigorous pheno) — Week 3 status Morphology: orthotropic apical dominance, moderate internodal length, strong lateral initiation. Canopy tissue: • Early week: interveinal chlorosis + marginal bronzing localized to older fans (classic Mg draw-down with ultra-pure water + K competition). • Late week: new growth fully re-greened, uniform lamina gloss; no tip burn. Stems/petioles: anthocyanin pigmentation (genetic; not deficiency). Rhizosphere: bright white, dense trichoblasts (root hairs), intact apical caps; slight suberization at crown (normal structural maturation). Verdict: Deficiency arrested; photosynthetic apparatus stabilized. Ready for modest EC/DLI increase in Week 4. ⸻ Plant 001 (compact pheno) — Week 3 status Morphology: tight internodes, strong cambial thickening (secondary xylem), high stem:height mechanical index. Canopy tissue: uniformly dark green; only residual lower-leaf freckling from early transition (non-progressing). Leaf posture: slight adaxial cupping on a few mid leaves (VPD adaptation, not stress). Rhizosphere: high-density lateral branching; “aeroponic fuzz” root hair carpet; immaculate white. Verdict: Vigorous and balanced; ~5–7 days behind 003 by genotype, but accelerating into exponential vegetative phase. • 003: Mg deficiency resolved; upper canopy deep green; strong turgor; no new lesions. • 001: Continuous healthy expansion; no active deficiencies. • Roots (both): white, turgid, high hair density; no biofilm; pre-pulse satin sheen confirms correct mist interval. • System: pH stable around 5.8; EC stable; Adjustments made during Week 3 • Top nozzles disabled (sputter risk) → bottom perimeter loop only. Week 4 Load Out: Targets: EC 0.85–0.90 mS/cm, pH 5.75–5.85, ORP 250–300 mV, water 64–67 °F. Reservoir formula (per 5.0 gal / 19 L) -Armor Si: 2.5 mL -Calimagic : 6.5 mL – slight bump for Mg to arrest early interveinal chlorosis. -Flora Micro: 16 mL -Flora Bloom: 16 mL -Flora Grow: 2 mL (kept low to avoid K-dominance and Mg antagonism) -Floralicious Plus: 1.0 mL -RapidStart: 2 mL -Purpinator: 10 mL -SLF-100 : 10 mL Biologics -Tribus: 1.25 mL -King Crab: 1.25 mL -Orca: 0.6 mL Day temp: 77–80 °F (25–26.5 °C) Night temp: 72–74 °F (22–23 °C) RH: 65–70% → VPD 0.9–1.1 kPa DLI: 20–22 mol·m⁻²·d⁻¹

Dudemann IRIE MAIDEN (ROMULAN GENETICS) week 4 status. Week 3 – Photoperiod correction: Discovered light on 24/0. Returned to 18/6 tonight. -DLI jump: With the same PPFD, going from 18/6 → 24/0 increases DLI by 33%. DLI = PPFD × photoperiod ÷ 1e6. -Transpiration & demand: More light hours = higher water flux and Mg/ Ca demand → can trigger interveinal chlorosis on older leaves and mild adaxial taco up top. -Circadian disruption: Mature clones prefer a dark period (phytochrome reset IM-001 (Compact Pheno) -Uniform medium-deep green canopy with upright petioles and no chlorosis. -Slight adaxial cupping on upper fans = normal transpiration control, not drought. -Tight internodes; strong secondary xylem at the base gives a sturdy stem. -Roots → bright white with satin sheen; dense lateral branching and glassy tips. -Mild basal suberization only – excellent oxygen diffusion. -Overall = healthy, compact, gaining vigor, about a week behind 003 but accelerating. IM-003 (Vigorous Pheno) -Lush medium-green foliage; older fans show only faint, non-progressing Mg speckling. -New growth flawless – no burn or curl. -Apex shows minor upward cupping from VPD regulation; petioles fully turgid. -Roots → snow-white, nacreous finish, dense trichoblast carpet, no biofilm. -Architecture → orthotropic apex with strong lateral initiation and balanced spacing. -Fully assimilating nutrients; vigorous vertical and lateral expansion underway. IRIE MAIDEN (ROMULAN GENETICS) — WEEK 4 VEG LOAD-OUT Stage: Veg Week 4 (Oct 26 – Nov 1 2025) Target EC: 1.05 – 1.10 mS/cm pH: 5.7 – 5.9 RZT: 66 °F Photoperiod: 18 / 6 DLI: 23 – 25 mol · m⁻² · d⁻¹ VPD: Day ≈ 1.0 kPa | Night ≈ 0.8 kPa UV bars ON for 30 min end-of-day (UVA 370–385 nm). No dehumidifier water - Water source: Tap water (EC 0.268 mS/cm) • Dechlorination: ½ tablet (≈ 250 mg) Nature Made Vitamin C (ascorbic acid), crushed and dissolved; neutralized all residual chlorine/chloramine within 10 min. Feed Recipe (Per 5 gal) -Armor Si – 2.5 mL -Calimagic – 6 mL -Flora Micro – 16 mL -Flora Bloom – 16 mL -Flora Grow – 2.5 mL -Floralicious Plus – 1 mL -Purpinator – 10 mL -RapidStart – 1 mL -SLF-100 – 10 mL -Tribus / King Crab / Orca – ¼ dose each Final EC: 1.095 mS/cm Final pH: 5.79