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Reversal of Lil' Rose

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4 years ago
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Grow Conditions
Week 1
Vegetation
11.43
cm
inch
Height
18 hrs
Light Schedule
10+ conditions after
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Commented by
Tragen353 Tragen353
4 years ago
The Project: This is an attempt to "reverse" (take a female plant and cause it to turn into a male) one my LCH clones for purposes of breeding feminized seeds. Any plants pollinated with pollen gathered from this plant (in theory) should be feminized and thus once bred with another strain should produce 99% female plants. The Process: So I have decided to try the colloidal silver method which forces the plant to change sex due to chemical stressors (not natural ones). I created my own silver with PPM of 19. I am spraying the entire clone down in the morning and just before lights out. I will continue to do this for one week then it will go into the flower tent. At that point I will switch to spraying once a day. That will continue for the first week it is in flower. Follow along if your curious..I know I am!!!! June 17/Day 2: So this is day two of this little pet project. This first week will be boring with not much to report. I will be doing a few pictures so I can document what the effects of this process looks like. June 18/Day 3: Man she does not like this one little bit. She is getting ugly. I guess part of the process. June 21/Day 6: Not happy but still growing. Will be moving her tomorrow and setting the spraying down to once a day. Have a little spot for her as she changes and grows in the big girl tent.
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blendmedmedman
blendmedmedmanweek 1
@Tragen353, ITEMS YOU WILL NEED WHEN MAKING STS: Brown Glass Bottle and Sprayer Top (Pint) Trash Bag and Tape Digital or Other .xx Accurate Gram Scale Distilled or R/O Water (2)500ml Brown Glass Bottles 2 Glass Mixing Containers (I used Pyrex 2-cup measures) Dawn Ultra Dish Soap or other plain soap/surfactant (2)60cc Syringes Silver Nitrate Sodium Thiosulfate (Anhydrous or Pentahydrate) Painter's Mask Gloves Mixing the A and B "Stock" Solutions STS is stored as two solutions, A & B. When kept sealed, cool and without exposure to light, they are very stable. Separately, they can be stored for months without issue. Once mixed their life span is about 1 month, but only if kept cool, sealed and in the dark. ;) This mix is diluted with r/o water before spraying on plants. Stock Solution A Fill the pyrex 2-cup measure to the 500ml line with r/o water Measure .5 Grams of Silver Nitrate using the digital scale Slowly pour the silver nitrate crystals in to the 500ml of r/o water while stirring Stir until dissolved (15 seconds) Pour the solution in to the 500ml brown glass bottle and cap it tightly LABEL the bottle "STS PART A" in a clear and long lasting fashion Thoroughly wash and rinse the pyrex measuring cup Stock Solution B Fill the pyrex 2-cup measure to the 500ml mark with r/o water Measure 2.5 Grams of Sodium Thiosulfate Anhydrous OR 3.9Grams of Sodium Thiosulfate Pentahydrate Add the Sodium Thiosulfate to the r/o water while stirring Stir until completely dissolved (30-45 seconds) Pour the Sodium Thiosulfate solution in to a 500ml brown glass bottle and cap it tightly LABEL the bottle "STS PART B" in a clear and long lasting fashion Throughly wash and rinse the pyrex measuring cup I store these solutions in the 500ml brown glass bottles and mix fresh before diluting for spraying. It is best to keep them in the fridge. ;) Mixing and Diluting the Stock Solutions Before Spraying ALWAYS Mix Part A in to Part B! This means putting part B in a container first and then adding part A while stirring. The measurements listed here make 1 quart, which fits the size of the sprayer and bottles I posted above. This amount provides plenty of solution for a 2' tall plant, sprayed every 5 days for 30 days. When stored in a dark cool place, the mixed solution is effective for approximately 1 month. The A and B solutions will be effective for at least 6 months. For 1 Quart of Solution: which will fit the sprayer listed: Use a 60cc syringe and suck up 50ml of Part B solution Gently squirt the Part B solution in to the Pyrex measuring cup Completely rinse the syringe or use a second clean syringe Suck up 50cc of Part B Solution While stirring the stock solution in the measuring cup rapidly (I use a small Stainless Steel Whisk), gently squirt the 50cc's of Part A solution in to the measuring cup. Go slowly, yet quickly enough to completely mix them within 30 seconds. REMEMBER it's IMPORTANT to DILUTE! Without dilution this solution will burn plants When the above directions are followed you will have 100ml of mixed stock solution in a pyrex measuring cup. Pour this solution into the 1 quart, brown glass bottle with the sprayer top and then fill the bottle the rest of the way with r/o or distilled water. (800+ml) This dilution rate is very close to a 1:9, stock:water mixture. Add 6-10 drops of Dawn Ultra dish soap (or other soap/surfactant, some people use yucca extract), shake gently and spray until the soap mixture is being sprayed. Test it on a leaf. The mixture should stick to the leaf without beading up and rolling off. If necessary, add additional drops of soap until the solution sticks/spreads well. You can also use Yucca extract as a spreader/surfactant, instead of the dish soap. (I cannot reccomend Tween-80 even though other have said it works. I personally cannot use it without having serious memory, mood and logic problems when exposed to the Polysorbate in Tween-80.) Light Proof the Sprayer Bottle! I know it's a brown glass bottle and a black plastic top, I still covered it further. Since I keep it in the fridge, there is definitely strong light when the door is open. ;) I put the sprayer in a black plastic trash bag and folded it in a few layers around the bottle, then taped it in place. It's not sexy, but it works. :) You can see it in the photo of the chemicals and bottles above.
blendmedmedman
blendmedmedmanweek 1
are you using the silver col.? method?? I myself have been using the silver thisolate, it works real good making a reversed branch, got tons of seed from a cross, I like it as you only spray the plant/branch once every five days at lights out, I made it myself really easy, the downside I have found it does not produce much pollen, in other words it worked but I could not collect for future use. I also made the other col. silver but I have not used it as of yet since the first worked. now I have used the col. silver like you I sprayed daily but it looked as if the plant was being burned?? the silver thisolate though did not and it did work 100% the cross I made was from a super skunk x eagle bill both by sensi. I got over 200 seeds from that, while the sprayed branch on the super skunk produced about 65 seeds. I have also breed seeds using males, and I brushed branches on select females and got seed and collected pollen. from the reg seed pollen run I learned the best time to get pollen on the bud areas was between 3-4 week flowering mark. just thought I would share this as your pretty much doing the same as I am, believe me there was not much info on this process, esp. the using of the silver thisolate spray. ill be following this one for sure, good luck!
Wicked_Stix
Wicked_Stix
@blendmedmedman, if your leaves are burning with the colloidal silver then it is too much light power. I use low watt cfl when I spray them. Interesting that you didn't get much pollen with the sts. I haven't tried it but have heard the same thing from other people.
blendmedmedman
blendmedmedman
@Tragen353, Some Tips From the Pros I've searched the internet for as much information as I could find on using STS and working with pollen. I found a few pieces of information which some of you may find helpful. The first two are mine. Trim Everything But The Top Three Nodes of Each Branch You want the plant to focus a lot of energy on perfectly formed flowers. Trimming off all of the undergrowth (before starting flowering) will make this happen. Trust me, you will get plenty of pollen. ;) Doing this reduces the possibility of mold and other problems. This also means, at harvest time, there's nothing to stop the fall of pollen except some branches. Spray Every 5 Days All over the internet you'll see people recommending spraying a plant and tossing it in flower and only a few say to spray it again after 2 weeks. The rest of the horticultural world says STS is effective for about 5 days in a plant. The rest of the horticultural world sprays every 5-7 days. I spray every 5 days. Starting 5 days before changing the lighting to a flowering schedule, spray the nodes of the plant every 5 days until 10% of the flower pods are open and dumping pollen. (Once the last spray is dry, lay out your collection trays, parchment paper or foil) Pods Which Won't Open Can Still Be Viable Spraying every 5 days, the pods should open well and dump lots of pollen. Should you find a plant which grows pods and seems to quit, right before opening, you can still harvest the pollen. Clip the pods off and dry them in a low humidity area or dessicant chamber. Over a piece of glass or parchment paper, rub the pods across a stainless steel mesh, like this small fine-mesh kitchen strainer. Pollen will drop through the screen, along with small bits of pod/plant matter. You'll also end up with plant matter which will not go through the screen. Rub until you're sure you've rubbed the majority of the pollen through the screen. A small brush comes in handy to clean the screen of pollen. Dilute Your Pollen With Toasted Flour When pollen is mixed with toasted (and cooled) flour, it greatly reduces waste and helps contain the pollen when using it. Using straight pollen is wasteful and adding flour greatly increases your chances of spreading the pollen thin enough for full use. Adding flour also helps keep pollen from floating around the grow area, pulling it to the floor. Toast 50 to 100 times as much flour as you have pollen in a dry pan over medium heat. Let it cool. Thoroughly mix your pollen into the cooled flour. I like using a stainless steel pan, becuse pollen and flour slide effortlessly across the bottom. Once it's completely mixed you can pollinate your flowers
blendmedmedman
blendmedmedman
@Tragen353, Spraying Methods and Schedule That being said, the process of treating your plants with STS is very straightforward. There's debate about spraying each node or using an eye-dropper on each node. I use the spray method and I know it works. Spray Every 5 Days After Lights Out Silver Thiosulfate stops the production of ethylene. This effect only lasts a few days in cannabis and spraying every 5 days keeps ethylene production the lowest. Turn off the main lights and spray before the night cycle. Silver thiosulfate is sensitive to light, so low lighting and then dark works well. Dilute Further if Burning/Yellowing Develops Some strains/phenotypes of cannabis are more sensitive than others. Should yellowing or burning develop, pour out some of the solution in the sprayer (with lots of rinse water down the drain) and add distilled or r/o water. Keep STS Out of the Root Zone Cover the base area of the plant with newspapers before spraying. Make sure the plant has completely stopped dripping before removing the newspapers or other barriers. Saturate Each Node With Spray The nodes are located at each spot where a leaf joins a branch. During vegetative growth, each node will eventually produce another branch. During flowering, each node produces flowers. Turn Off Fans Before Pollen Begins Dropping Pollen is insidious and will float in to every crack and corner of your house. HEPA filtered ventilation (even HEPA rated furnace filters work) will significantly reduce the spread of pollen. Spray down all pollen covered areas with clean water to deactivate pollen. Dilute Solution if Plants Show Burning A slight reaction is fine, while severe reactions require diluting with additional distilled or r/o water. Open up the spray bottle and top it off with about 1/3rd of a cup of distilled or r/o water. Pour a bit out first, if there won't be room. What You Should Expect to See You sprayed down your female, gave her 5 days more veg and sprayed her again. You changed the lighting to flowering and have sprayed her every 5 days for a couple weeks. Here's an example of what you could be looking at. In the photo below you can see the male flowers, the parts which look like balls or seed pods, and also the white pistils of the female flowers.
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blendmedmedman
blendmedmedmanweek 1
ok I think that should do it, real easy to mix and spray
blendmedmedman
blendmedmedman
@Tragen353, very nice, so how many times' do you think you sprayed using that solution? yeah im interested to see, it seems like a lot but once you process the mixes and fill the spray bottle its not too much both the chemicals are under 20 bucks and makes a ton, the col. silver was pretty easy as well. ill be watching yours since that's what your using and im hoping you can collect some pollen, it is a good feeling
Tragen353
Tragen353
@blendmedmedman, Yeah a lot to take in. Great info! Been super busy lately and sorry took so long. Have not had time to update or even take many pics. I have been spraying at lights out despite it saying I stopped. Have not updated yet. Saw some pistols popping and sprayed until today. I actually think the change is in effect and will get new pics this evening.
Wicked_Stix
Wicked_Stixweek 5
Very exciting! I got some clones that are getting ready to drop some pollen too. Crossing strains is a lot of fun.
Tragen353
Tragen353
@Wicked_Stix, Yeah Bro! Have some Jack Herer seeds and some Amnesia Haze. Wanna try to make my own J1.......I love that strain! This pollen (LemonChello Haze) I wanna mix with my Strawberry Banana Grape.....Call it Fruit Bowl...lol!
Ferenc
Ferencweek 6
Exciting mate! @Tragen353
GYOweed
GYOweedweek 6
I was just drinking the LeCrox soda Lemonchello lol Good job being one of few to display a revesal diary. I haven't done an STS job in ages but wanted to make a Lemon Strawberry strain. I need to sts some DNA Strawberry Sorbet next season for sure. Seedsman has been pretty cool about sending me seeds. I notice you need to grow at least 3 healthy ones to pick the pheno that is "right" from them but the ones that come out good are frosty, tasty, and high yield. Have you done their other tasty strains yet? CBD cream n Cheese was very yum too.