Your pots appear to
be large, therefore
if you're going to avoid transplants by placing seedling directly in its final pot, do not saturate completly the water. It only needs a shot glass of water It is important not to use use soil amendments that require a pre-cooking period of 30 days
such as blood meal & other
meals & guanos, amongst others. Otherwise roots will get burnt.
If you used small pots, yes it would be a good idea to activate your soil's microbiology beforehand