Oh shoot.... I was really interested in reading Nugsery's answer talking about air being mostly (78%-ish) nitrogen... must have deleted that one! LOL! And then gone to some site and copied some blahblahblah about using CO2... oh dear!
I can't answer your question, unfortunately, because I don't use CO2 at all because improper use/set up can be highly toxic and may even kill ... But I was curious about your question and so ducked in here...
Good luck!