Lots of great answers and info. 2 cents to add.
Helped me to understand what CO2 does naturally indoors and how it changes over a daily cycle. We have photosynthesis by day that eats up all the CO2 it can find; at night, the plant begins cellular respiration, ejecting CO2 back into the tent. It has a small footprint at first, but as she grows more and more, CO2 is produced overnight. Early mornings in a tent will have maximum CO2. Now, when the plant is small, there is not much created, but in a 4x4 in the full swing of flowering, she can sit anywhere around 1200-2000ppm by her own creation alone at lights on. As the day progresses, she eats up all the CO2; come nighttime, the tent is depleted and can get to 4-600ppm. (CO2 is dense; I read a lot of people say that an exhausting tent will deplete the tent, but not with a bit of creative planning and understanding of HVAC, it won't, like everything else its just all pressures, switches and signals and finding that sweet spot through trial and error one way to do it)
Why is this important?
The CO2 compensation point (Γ) is the CO2 concentration where the rate of photosynthesis equals the rate of respiration, meaning the plant neither absorbs nor releases CO2.
In a closed system, as the CO2 concentration decreases, the photosynthetic rate of illuminated leaves will also decrease until the rate of CO2 uptake for photosynthesis equals the rate of CO2 release from respiration.
Don't supplement CO2 at night; oxygen is a requirement at night, the same way CO2 is needed during the day. Although available in much larger quantities than CO2, it is still something that can run short if proper airflow is not given at night, especially in smaller, more restricted spaces.
When CO2 levels increase, plants respond by partially closing their stomata, reducing water loss through the stomata.
High Co2 overnight will greatly restrict cellular respiration when combined with low Co2 environmental metrics. Especially so when people let RH sit at 65% all night, further crippling gas exchange.
This is also why you need temps up in the 86F-93F range during daylight to utilize CO2 above 1000ppm. You don't realize how much water she burns through to cool until you try it. Chlorophyll needs replenished at a much higher rate than normal; this requires an increased magnesium dosage to deal with excess replenishment of chloryphyll across the plant. possibly a wee dose of nitrogen needed too. With massive levels of transpiration running high CO2, whatever VPD calculations are made for daytime should be made with a -5F modifier for LeafST; this doesn't apply to nighttime respiration.
You are running 1000 µmol m-2 s-1
@80F
at 1000µmol m-2 s-1 over 12 hours is 43DLI, plant can do 40DLI
@400ppm.
Look to hit around 60DLI if you elevate CO2.
You're not in the range to make use of elevated CO2, but the elevated CO2 is reducing the stomatal aperture or the ability to perform gas exchange without the environmental conditions that would come with levels of CO2 that high. Gas exchange is responsible for the water column cohesion plant's ability to pull water through the plant.
Wonder why your car's not going fast because it's in the wrong gear.
On a 12-hour daylight, look to have a dedicated 4-hour window pushing environmentals to match elevated CO2 at 1800µmol m-2, both sides of that have normal 900-1000µmol m-2 with normal environmentals to match. Only supplement CO2 during a 4-hour window when its ballllllls to the walls to make that extra 20 moles. Do not supplement at night,
at 400ppm 40moles in a day
At 1,800ppm, 60moles in a day, or somewhere about.
If plant sucks in more CO2 than it produces the medium will become a carbon sink, more carbon = more moisture retention. This links into to sugar signalling complexes, then you start to learn borons role in all of that and on and on and on like layers of a endless onion.
Good luck Wiz