First you need to understand which method you grow, synthetically or organic as different growing methods have different variables that will require different approaches to "flushing" per say.
Synthetic growing you don't need to worry so much about the microorganisms, just flush that medium till it's empty. Thing to remember thoough is that different forms of nitrogen have different mobilities within the soil itself with nitrates no3- being very mobile and nh4+ being very immobile. To separate ammoniacal nitrogen from soil particles it takes 4 - 5 times more water. This is often overlooked and will prevent a autophagic response.
Flushing essentially strips the medium of all salts. Autophagy in plants is strongly induced by a low carbon (C) to nitrogen (N) ratio.
Thing about nitrogen is it's required for nearly every single enzyme or amino and it's also used to feed the microorganisms which break down sugars (carbon). A plant that detects a shortage of nitrogen will begin to recycle nitrogen from other parts of the plant and use it to feed the microorganisms that ensure oxidative phosphorylation occurs. If that goes then plant dies or runs off 10% , no nitrogen, no survival. Response Inititated.
The nitrate ion NO3-is already in its most oxidized, stable form with a nitrogen oxidation state of +5 . Because it is already fully oxidized, it cannot undergo further oxidation and will never oxidize no matter how long you dry or cure it will never evaporate and you will smoke it.
Inhaling nitrates will cause yuck, headaches and nausea.
During leaf senescence in plants, this process is critical for remobilizing nitrogen from aging "source" tissues to developing "sink" organs like seeds or new leaves, significantly impacting nitrogen use efficiency and crop yield.
Autophagy, a critical nutrient recycling process, is linked to nitrogen reductase (NR) in plants, primarily under low-nitrogen stress. Autophagy regulates NR activity and expression, thereby optimizing nitrogen assimilation and utilization.
Nitrate Reductase (NR) is the crucial first enzyme that starts the conversion of nitrate NO3-into other usable nitrogen forms THAT WILL OXIDIZE during the dry/cure.
Soon as plant is harvested NR will cease to function and nitrates will become volatile and you will smoke them.
"-- flushing is bro science myth.. it's modern day mysticism". Beyond your ability to articulate is more accurate.
Flushing itself, historically has been just Flushing everyth8ng out the medium. Yes its 100% true thay Flushing the entirety of the salt ions out of a medium for 2 weeks prior to harvest will drop yield like 10-20% and have a impact of final flavourful and smell. This is not.up for debate.
The plant still needs to fill those trichomes, plant still has ripening to do, the entire concept is that with a deeper understanding of the underlying mechanisms you can initiate autohpahy by removing almost all.the nitrogen and still have the plant firing on all cylinders, using the carbon to nitrogen ratio.to induce nutrient recycling without sacradicing the yield taste or flavour.
No one is saying it's easy-to-use it certainly took me years to get to a point of even having the knowledge to attenpt., but to deny it existence is absurd. With an effort to simplify the entire process let's jist get everyone to grow synthetically and flush everything, that way everything is simplified and no one needs to knee jerk BRO SCIENCE response.
Smart people learn from everyone and everything, ordinary people learn from experience, stupid people have all the answers. Anyone needs proof of concept go check my latest diary with 200 views. :) CH#13.