2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

seems to really want to turn into a bush so i’m just going to let it do its thing!

day 49 just pure water phed down. it is day 51, morning before lights on, I might chop today. I use a zero water filter pitcher got off walmart. My humidifier is only a sonic wave. If you use regular tap water it will get gunky in a sonic humidifier. If you have a heat humidifier that boils the water the mist should be clean. These are the 2 types of water humidifiers. Researching best water filters this "zero water" also comes with a free ppm meter! yay! Other water pitcher brands; the water still has ppm. So water misted will still have ppm contamination. You can easily used distilled water only, and reverse osmosis. I just fill a 4gal office water jug with the pitcher and fill my humidifier when needed. I do not use a humidifier in my entire grow up to this point when I chop, I need the perfect rh or this whole effort can fail. growing up to this point only requires 20% effort, chopping now for the 2 weeks dry is this 80% of crucial determination, succeed. A lot of tasks are required today for a clean chop. I feel confident, observed my cross seeds with pink paradise and one with a slightly open caylex looks dark enough for successful germination. I figured 8-16 seeds may be created, 3-4 buds were potentially pollinated. Many growers don't understand when creating genetics with female/female there is a slight chance of herm. You lose 50% of your genetic function. Just water last 2 days, no humic no folvic, 1st time entire grow ha! There is a lot of cleaning to do, remove the trees, clean the entire tent and environment with bleach water, hacksaw. I am very proud of my grow. I have sampled 3 of the strains, dr. thunder legit made my lips numb but it could have just been the terps removing my skin cells from the sticky filter, no lie. This is my best grow to date. I did this for you, and to document hype or not under the best conditions I could give my plants, full max potential, although I wish I had more lights, I am not looking for industrial lol, this is how I grow. Learn a lot on youtube and podcasts there is many good content and I may dab into it. You can have a room full of growers and you have 1 seed in your hand, they will all tell you that their way is correct ;) and the best way. Oreo is stinky AF, Bridal is just amazing glad its massive, just not into sativa but the test seemed slight sativa with majority indica, pink untested, dr, thunder just a lip numming plant aha, its very nice, all 4 amazing appeal like advertised, and excited for next run. Either crosses from this or other copycat genetics. I will give you beautiful glamout shots today, the sizes of the plants, My guesstimate bridal 1lb, thunder 1lb, pink, 0.4lb, bridal 0.5, I am ambitious off 640w lm301h, and 4x 20w blurples daisy chained. day 51 4 chopped see you in a few weeks :D

Well I have 6 little seedlings that sprouted looking happy and healthy.Going to give just plain water once the pots dry up a bit more.Ill add Gaia green 4,4,4 top dressing with a shot of recharge in a few days after these seedlings are stronger.Se7000 set at 30 percent power and is raised to the ceiling.I prefer to do this over lowering the lights and intensity and not being able to walk around and work in the ⛺️.Day 4 added a 1 inch layer of farm farm ocean forest and I’ll water plain ph tap water when the pots are dry.Also placed some pins and anchors to tie down branches for when the girls are ready for training :)

Hello 👋 👋 👋 As a 29th day arrived inside the tent there are many changes 😇 💪 🙏 Purple and Cinderella start flowering a week before Bruce Banner but all girls have got similar high 😇 💪 🙏 63 cm was taken from B.B 😁 All of my beauties expanding in size, flowers sites start to taking a mass and density. Some brown spots shows on humidity effected leafs so I removed them to control a healthy conditions 💪 💪 💪 Update: Day 30 Ladies definitely goes in flowering stage, every day I can observe a massive size and high changes. It's look like the tent will be full of buds soon. I'm very happy to see a purple colour on my Purple Lemonade. I'm excited because this is my first purple strain 😇 🙏 😇 Day 32 I have to move a girls a little to adjust them for better space use. I love to look on them 😍 Spec. Mars-Hydro TSW2000 100% power RAM mix flow 199m3/h Humidity 58% - 65% Have a fantastic day, You Lovely Girls Growers 😇 😇 😇

Looks like she will be the first to ripen. Probably 2 weeks left on her. Lowered to temps to 72. Will start flush when I see some amber.

Entering the 8th week and she is really doing fantastically! When compared to the first Black Jack I grew, with just LST, the differences are clear and impressive. The bud sites are easily 4 times as much. She is almost as tall as the first one was when I harvested her, and she is still only just beginning to flower. More stable and way easier to maintain after the mainlines are established. Super impressed and happy with the results and will absolutely utilize this technique more in future. Great results and super excited to see how big she gets to finish budding. All great things. Cheers

Sehr erdiger geräucherter Geruch

Day 30 Wed Add A&B 5 ml and water PH 5.5 - 5.8 EC 0.6 - 0.7 DLI 18h PPFD Water 24 Day 31 Thu Add water, Ph Up PH 5.3 - 5.9 EC 0.7 DLI 18h PPFD Water 22c Day 32 Fri Add Water PH 5.2 - 5.8 EC 0.7 - 0.6 DLI 18h PPFD Water 22c

Welcome to Day 8 things are going very well so far the plants are taking on a lot of height and mass for only one week's growth. Not a whole lot to talk about really early stages of growth really not much happens. I will say that I've transitioned to a completely Advanced Nutrients feeding system for this week Day 9 transplanted the girls into their final five gallon pots root development is extremely well for literally just over one week into veg Day 10 the girls have clearly recovered very well from transplant there are growing strong and healthy Welcome to day 14 last day of the week I decided to micro top as the ladies were ready. We were at the 4th node that's typically when I make her top at this point I'm not sure how many toppings I'm going to do but I don't want the plans becoming too large for the tent by doing too many tops

Welcome to week 13🤠 This week I have been doing a bit of LST every day, so I've been bussy. I have also trimmed her a litlle bit, but not to much She's comming to a halt in growth, so very soon the buds will start swelling up They have a very sweet smell, something like vitamin drinks, and the buds are getting sticky Watered her with ph7.21 water, run-off water was at ph6.39, So I still have to raise the pH Weather was worm this week and very sunny, but it should cool down again She's very symnetrical and beautiful, I really like relaxing next to her❤️ I've also got all the material for my drying box, so hopefully I'll make it soon And I'll be making a small roof so my harvest doesn't get ruined So I think this is all to be said, I'll edit this week if I forgot to say something or made some mistakes Otherewise see you soon!🤠🤠

❄️👋❄️ Depuis quelques temps je ne vois pas beaucoup ma culture car j'ai déménagé et qu'elle ne se trouve plus chez moi. Je passe la voir uniquement pour l'arroser, environ une fois par semaine. J'ajoute du Bioenhancer une fois sur 2. Malgré mon absence les plantes m'ont l'air d'être en bonne forme. Pourvu que ça dure ! ❤️️🤞 Intensité de la FC3000: 90% Ventilation : Extracteur mars hydro 6 pouces avec filtre à charbon puissance : 4/10 (24h/24h) + 3 ventilateurs à l'intérieur ( ON 8/24h). ils s'activent à un horaire différent. 🌟Venez me voir sur Instagram 🌠 https://www.instagram.com/hou_stone420/ 👨‍🚀❄️❄️❄️👋❄️❄️❄️☄️❄️

💩Holy Crap We Are Back At It And Loving It💩 👉FOLKS WE R ALMOST TO THE FINISH LINE👈 Growmies we are at DAY 63 and she's just killing💀it👌 👉We are in full flowering mode and she's doing great 👈 Everything is looking good even the Tiny Cup 🥤 So Shit , I gave them just a tad to much nutes at the start of feeding 👈 But I have since fixed it So I'm still doing some low stress training 🙃 and some defolation 😳 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Salutations, fellow cultivators! Week 2 unfolds with the grace of a symphony, and the tale of my Blackberry Auto continues to captivate. The journey is both an art and a science, and every green leaf is a brushstroke on the canvas of cultivation. Our heroine has found her throne – a final 16L AutoPot. What makes AutoPots the crown jewel of cultivation, you ask? These self-watering wonders provide a dynamic, gravity-fed watering system, ensuring the plant receives precisely what it needs when it needs it. It's the epitome of hands-off perfection, allowing the plant to dictate its own hydration destiny. Transplant day is a ritual, a moment where roots meet Mycor Mix magic. This mix, a blend of mycorrhizae species, becomes the plant's ally, expanding the root system and fostering nutrient uptake. It's like giving my Blackberry Auto a superhero sidekick, enhancing her abilities and resilience. The transplantation dance continues with the application of Aptus Holland Mycor Mix on the roots and surroundings. This isn't just a transplant; it's a ritual of care. Mycor Mix establishes a symbiotic relationship, unlocking nutrients and fortifying the soil with beneficial microorganisms. It's a small act with profound effects, ensuring my plant's journey is supported at its very foundation. As the pot receives its royal guest, the soil is adorned with 75g of Aptus All-in-One pellets (NPK). This powerhouse blend provides a balanced nutrient feast, a banquet for optimal growth. The soil is further enriched with Aptus Micro Mix, a harmonious supplement that fine-tunes the nutrient symphony, ensuring every note is in perfect balance. Post-transplant, the ritual concludes with a nourishing bath – my Veg Aptus Recipe. This elixir, carefully concocted, ensures a smooth transition for Blackberry Auto. It's not just water; it's a potion that whispers the promise of health, strength, and vitality. The week embraces low-stress training, a dance where leaves are gently bent to shape the plant's architecture. Blackberry Auto, the prima ballerina, responds with joy. This technique not only encourages lateral growth but also maximizes light exposure, orchestrating a symphony of photosynthesis. As I witness this botanical ballet, I'm reminded that cultivation is a dialogue between caretaker and plant. Together, we embark on a journey where each element, from AutoPot elegance to Mycor Mix finesse, plays a vital role. Stay tuned for the continued saga of Blackberry Auto – a story told leaf by leaf, in every graceful twist and turn. Genetics Blackberry Auto @Fast_Buds Food - @aptusholland @aptus_world As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and blessed with you all in my life

Day 91 and all 4 plants have been on ripen for 8 days. That has helped speed up the lemon pie so I will get something from her. I spotted a good few what I think may be spring tails swimming in the res, as its near my res change and I'm flushing after that I'm not to worried about them. Pineapple express has been bulking all week and trichomes are all cloudy, she looks ace. Jack herer also bulked up a bit more, she has cloudy and a few amber trichomes, she is ready for the flush. Gorilla cookies has put on a little weight this week, her trichomes are cloudy and Amber and lots of them, she was ready to flush a few days ago really. Lemon pie the one that's looked poorly all the way through has turned into a Swan. Colours have come out nicely and bud production is coming on nicely, she could do with another 5 to 10 days before flushing but I'm finishing her with the others. 2 more days on ripen then I'm starting to flush. Not long left. Max 25.8deg Avg 22.1deg Min 17.2deg Max 59.6% Avg 47.4% Min 37.5%

Seen some explosive growth in the past week, absolutely incredible. The damn UKBS 2 is nearly 20" tall - it's already seemingly recovered from the topping I gave it the other day and has started to take off yet again. UKBS 1 is just kind of existing, it's growth is quite noticeable as well but when compared to the other 3 plants is somewhat negligible. I suppose that'd have a lot to do with the much smaller pot that UKBS 1 is in compared to any other plant in the tent. The carl plants are both doing incredibly well, however the one in the 5-gallon fabric pot (Carl 1) is expanding horizontally like crazy; which is great. I think it may end up being one of my indoor plants depending on the size it gets to once everything starts going out. As usual, I'll update the text throughout the week. -3-28 - 3-30 - Been noticing a creeping deficiency/PH issues affecting UKBS 2 but also slightly UKBS 1. Unfortunately the drainage on UKBS 2's pot is horrible and may be contributing to a salt buildup in the soil. Can't wait for the correct weather so I can put her in a big ass fabric pot outside.

Week 9 of Flower - Beautiful colours and a crazy smell