Start of week 2 ladies are growing like beast gave them all botanicare kind bloom and base also gave them open sesame from fox farm line just gave 1/4 of the recommended dose. Was using Gaia green bloom but got a dwc grow going now and the botanicare is working better than anything I've seen before. 1st week went very well with the botanicare feeding plants really love it not sure what I'm going to do as the tent is way 2 full and stretch is not done yet.

Hola cultivadores. Se acerca la víspera de año nuevo, y con ello, la llegada de la floración. El día 12/27 observé las plantas y se aprecian diminutos pelos blancos, no están presentes en todas las ramas. Pero es suficiente para comenzar la fertilización con productos de floración. Escogí Bio-Bloom de BioBizz en su dosis media para que se adapte poco a poco al cambio de alimento, reduciendo igualmente el Fish-Mix a la mitad. Se mantuvo el Acti-Vera y el Alg-A-Mic en su misma dosis. Ya la planta alcanzó los 101 cm, esperemos crezca y engruese sus ramas porque igualmente están delgadas para soportar el peso de los cogollos.

2/10: I watered today with about 3/4 gallon each, plus cal-mag, signal, bembe, armor si, a little open sesame, and their final dose of endoboost. I took photos today, but forgot to snap a shot of the Soooperrunt. She's as tall as the short one now, just not as bushy. I think she'll make a fair amount of bud despite her sloooow start, smaller stature, and smaller pot. The tall one is keeping pace with the biggest plants in the garden. 2/11: Wife home sick today....postponing construction project to raise the lights....kinda scary....about 10"-12" from the tops now.. 2/12: I woke up to find the tallest of these bitches stretched another few inches and within 9 inches of the lights!!! I quickly drug everybody out of the closet and undertook the project. In addition to that project, I installed and hooked up my new AC Infinity 6" intake fan. It's pulling in fresh air from the soffit vent on the eave of the attic, and currently feeding the garden with 46f fresh air. I'm able to easily maintain daytime temps in the lower 70f's now. I am able to drive the nighttime temps as low as I want. The only issue is that the outdoor RH varies quite a bit, so I ordered a 30-pint dehumidifier to put in the top of the closet. It will battle with the evaporative cooler while the lights are on to keep it at 45% RH, but after the lights go off, it will lower the RH to 35% unti morning. After another couple of weeks, I'll kill the evap cooler altogether and try to maintain 35% RH 24/7. I'm optimistic that it will be the difference-maker in maintaining lower RH while I'm flushing all the plants during the last couple of weeks. That's usually such a challenge...especially with a bunch of 5 gallon pots. I also sprayed everybody with boomboom spray to try and mitigate the light burn damage that is likely to ensue. 2/13: Still stretching... about 12" from the lights again. I will wait to raise the lights until tomorrow when I feed them. I'm seeing calcium deficiency on a few plants, including a #9. Will up the dose tomorrow when I feed. 2/14: I fed them today with about 3/4 gallon each including grow big, big bloom, tiger bloom, cal-mag, signal, bembe, humic acid, and I switched over from Open Sesame to Beastie Bloomz. Raised the lights another couple of inches. I did some training on them and defo'd a little bit. 2/15: Installed the new dehumidifier and rigged the continuous drain on it...works great. 2/16: I rotated the edge plants and removed some old leaves. I added another 22w 3000k 4' bar light under the canopy. 2/17: I rearranged the garden and defoliated a little bit. That's it for week 8-

Day 30: Girls are stretching and starting flower😇 pots are getting dry quick so maybe ill have to up water to 1.2l once a day or even out through out the day🤔 Day 31: Gave about 1.3L feeding to all girls! made sure to hydrate coco all the way and gave some water to watertray so they can suck it up if they are still hungry! i cheked ppm on my water but didnt check runoff yet. ppm was 900 with 0.5 convert. ph around 6. will add pics later or tmrw😎 Day 32: gave girls little lighter feeding, around 3ml/L GH nutes b clmag. I was too lazy to take proper pics with better light so took a vid instead😁 Day 33: i was thinking of pinning the tallest cola to scrog but decided against it. Mainly because these girls are doing very well, and my only consern is light burn. I cant get my light as high as i want to, cos my tent is only 180cm and got filter above my light. I think ill need yo setup next grow so my filter is outside of the tent🤔 thx for all commentors on my growquestion/diary! Made my day to hear my babies are doing well without any bigger problems😁💚 Edit: did some LST on bigger colas to avoid lightburn since these girls are strecthing almost 5cm a day😁 hopefully they will keep the same energy on developing buds😉 I checked ppm and pH from runoff; #1 ppm 750 ph 6.6 #2 ppm 750 ph 6.5 #3 ph 6.4 ppm 750 (all ppm converts were made on 0.5 rate) Gonna give low ph watering next day Day 34: Good morning growers😎 this morning i gave my girls quite an heavy feed, 6ml calmag, micro, bloom and 3 ml gro into 5 or so litres. pH around 5.7 and ppm 1100 with 0.5. Pics are coming later afternoon with some runoff numbers😎 stay lifted💚

харвест прошел удачно!!! захарвил, потримил на мокрую положил в темный ящик на 4 дня и иногда проветривал, затем убрал в банки вместе с пакетиками integra.

Overall pretty happy with the grow, had a little mold issues but humidity was way to high for a while.

Woche über haben sich die Blüten weiter entwickelt. Die Trichome wurden mehr und man fängt an erste Terpene zu riechen. Wir haben Donnerstag (BT 2) stark entlaubt und schauen jetzt wie die Pflanzen wachsen. C.C ist bissl kleiner aber trotzdem hat sie starke Triebe und scheint gute Blüten auszubilden. Der Geruch ist aufjedenfall schon funky.

I got busy and missed my week 2 of flower update. But here’s week three. This strain has been putting in work. The stretch is decent and I feel like they still have a little more stretch in them. I did a little leaf stripping but planning on doing a lot more in the next day or so. There is a decent amount of tops coming through the first layer of trellis. I’ll keep the girls happy and hopefully she’ll make me happy on harvest day. All in all no complaints about this strain as of yet.

Day 26. All good.

The week before the magic happens

Not there yet checking trichomes starting this week. Probably have 2-3 weeks left. The strawberry pie continues to outshine the other strains. Gorilla glue is a close second with nice dense buds but not as many trichomes. The grapefruit may be a late maturing plant it seems like it’s starting to stack up but has a long way to go I’m guessing 4-5 weeks so not so impressed with the grapefruit for an auto. The grapefruit is acting more like a photo as you can see in the pictures the tallest plants are grapefruit. Only time will tell but I like autos for there fast turn around time if I wanted a plant that takes 12-14 weeks I would get photos and get bigger yields.

Just starting week 8, 1 white widow is growing excellent, the 2 blueberry are in back and they are full of flowers. The 1 white widow in front right is slow since the get go. Not much lst on her but I think she stunted and slowly flowering. Blueberry plants showing slight burn on leaves but still just using ph water on them for one more and back to nutes then. Just went down to do nutes and I figured out it was a cal mag deficiency not Nute burn, hopefully they can make it through this hiccup.

Day 8 Another day, another cm of vertical growth. The Blumat Digitals showed that the soil moisture was still in an ok range (115-131 mbar) but the topsoil was a bit dry so I sprayed each plant with 1 liter of water (pH 6.3). Day 9 The leaves are getting wider with no additional vertical growth. The right plant is noticeably larger but both look healthy. Day 10 Plants are a bit wider but all in all pretty uneventful. Day 11 Another day of the plants are getting wider but no vertical growth. The cover crop on the other hand has been growing quite a bit so I trimmed it down a bit. Day 12 The Blumat Digitals showed that the soil moisture was in the 114-162 mbar range so I gave each plant around 1.3 liters of water. Dumb as I am I forgot to pH the water though so I guess it was around 8.0 as that is where my water normally is. Oh well, gotta remember it next time. I also removed the humidity domes as the leaves of both plants started to touch the side of the domes. Day 13 The right plant is clearly larger than the left plant, being both 25% taller and 40% wider. Let's see how it progresses from here, maybe one plant is simply slower or maybe it's a different phenotype. Day 14 Not much to report except that the right plant is still growing at a fast rate while the left plant is creeping along. My girlfriend was transplanting our tomato plants today so I put a few in the tent as there was some extra space. I sure hope though I didn't bring in any silly bugs into the tent. Oh well, let's see... We keep the rest of the tomatoes and chilies under a ViparSpectra light in our front room until the weather finally gets good enough to move all the peppers and tomatoes outside.

I have to be carefull to lift her lol

🌱Day 58. Constantly keep watering with plain ph balanced water. Zero nutrients. Super soil working well.💚 🌱Day 60.💚 🌱Day 62.💚

Day 63: This should be the last week in veg. It might be a partial week...I'll make the call in a few days. I'm excited to see these babes flower!!! Day 64: Last night I saw what appeared to be signs of overwatering, so I turned off the humidifier and the temp increased immediately and today, they look just fine. I also raised the 150W LED on that side of the tent just a bit to reduce the intensity. This will be the last week for veg. I flipped the other tent, but these babes need just a little longer before flipping. Day 65: They looked pretty thirsty today, so I fed 1 gal of late veg nutes per plant. The soil was very dry according to my meter, so if I overwatered last time, they had plenty of dryness today. Day 67: I came home to near wilting plants! It had been 52 hours since last feeding and I've never seen them this thirsty during this grow. I watered 1 gal/plant again and GZ is showing the same signs of over watering again. The leaf color is fine with zero nutrient issues, so I suspect there may be a root problem. I don't know what else it could be? The photos are about 4 hours after watering. Day 68: The moisture meter showed bone dry again, so I watered a half gallon of water/cal-mag. I also decided to turn off the extra 150W LED sitting above her and just let the main LED do the work for now. Day 69: She was dry again today, so I watered a half gallon of late veg nutes and she's appearing to be recovering just fine. I think the heat in the tent might have been causing some issues as well, so good thing I turned off that extra light for now. Day 70: Last day of VEG!!!

The week has been going very well plants are reacting good to nutrients and seem to be happily growing , haven’t fiddled around too much this week as starting to get the hang of things slowly ! The grow so far has been quite smooth And haven’t run into any Major problems all plants have been topped and will continue lst throughout I believe they look quite healthy haha ? First timers luck 😉🤨 we hope so Good end to the week next week I shall trim and see how that goes Happy growing 💎

So this is how things are looking for the ladies at the end of Week 6 of Veg, I have uploaded a video for you guys with all the information, any questions just ask away 👍🏾👊🏾😎

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.