Hi Growbuddies. We are now into week 7 of this flowering period and all going well so far besides this diary app keeps resetting my update !!!. 3rd attempt here to upload

Week 14/4 - 20/4

soo little update : the 2 plants on the right side are probably not Jelly Pie #7 but rather Original Clon from Blimburn... there was a mistake when I got the cuttings. On the other hand the 2 plants on the left, I'm totally sure that they are so strong smelling and so pretty

Hey huerteros pues asi va esta sativa dream que hoy hace su dia 46... y nos veas como esta desarollando! Seguimos aplicando LST ... para ir abriendo la planta y haciendo una buena estructura para cuando llegue la fase de floración!! De momento solo regamos con agua y cada 10-15 dias añadimos microbes! El sustrato es All mix de biobizz y ya viene prefertilizado asi que por nutrientes no sera y la planta asi lo demuestro con ese verde tan vivo! Esperemos que todo siga igual... iremos subiendo contenido asiduamente todas las semanas! Saludos desde el sur 🇪🇸

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

10/10

This week I transplanted them from the AC Infinity Seed Starter to their 5 gal grow bag or their final pot! I like to make my own super soil out of Root Organics Formula 707, Coco Coir, Perlite, Vermiculite, and amendments. Then I placed a hole in the middle of the 5 gal grow bag, for the plant being transplanted, and placed the young seedling into the hole. Then I lightly covered around the base of the young seedling. Then I watered with 250mL of pH balanced H20! From there that's it just checking on it daily and watering as needed daily with plain pH balanced H20. Then I amended the soil with 4-4-4, 4-6-3, Blood Meal, Bone Meal, Alfalfa Meal, Kelp, and Earthworm Castings. Then I gave nutrients every other day. First General Hydroponics MaxiGrow then plain pH balanced H20 the next day. Then amended the soil as noted above. Then plain pH balanced H20 the next day. Then I would feed The Stash Blend and then plain pH balanced H20. Then the General Hydroponics MaxiGrow and then plain pH balanced H20. Then Real Growers Soil Recharge and finally plain pH balanced H20 to finish the week off. I always pH at 6.0 for nutrient solutions and 6.8 for my plain pH balanced H20. My temps for solutions were 72° and ppm was 800-850 with EC 1600-1700. I always use my oxygenated H20 that is monitored by a professional meter and so are it's levels! I have oxygenated H2O with a pump ready to go at all times! This way I can just scoop up the water and go on demand without having to worry! All and all they're growing well and gaining a ton daily!!

we transplanted the little ones in a bigger pot and a little bit more light also , the little freak is still alive and growing alittle bit slower than the rest but i will keep it as long it grows and who knows

29дней Сделал дефолиацию и растянул ветки. Сегодня впервые внёс Удобрения. Жижа универсальная 1.4г/л.,бад монстр 2мл/л по листу.

Primera semana en un sustrato malo del cual fue trasplantada la siguiente semana

Hey all! 👋 Remember last week when I said Falco was getting some chlorosis? Well, the first photos show how bad it is (or was) but the plant is "old", she was quite special as a young plant and her main leaves are doing well so I suppose what is happening is just something normal or maybe some other weird factor... might be affecting her, but more probably I'm just way too worried. In my confusion I added 2ml of Green Grow (which is the growing fertilizer I previously used) thinking that she needed the extra nitrogen but in the end it seems it was unnecessary (as it's a normal process of the plant), I just removed the old leaves and left her to be. 😝 Besides what I previously said I saw a lot of bud progress this week (and I tried to picture all that! 😋). There are plenty photos of the same flower (most of the times!) showing how trichomes were constantly appearing during this week. Next week I'll be adding some extra Potassium and Phosphorus and I hope those products will help my babies to get bigger buds! I also made a video at the end of the week to show how my plants look at a normal speed plus two time-lapses. There are also some photos I took with my DSLR and an inverse ring just to see how the plant would look... and I think they look amazing! 😍 Anyway, I hope you all like the photos and thanks for reading! 👋

Today is day 72 from seed!! This week went real well , 2 of the Forbiddin Runtz have been getting flushed an the rest will also start flush through this next week, I can’t believe the smell of this combo strain,, super fruity smelling an all are super sticky!! Stay tuned for next week yall !

Week 13 : This wraps up the last week of flush for the biotabs, they got 2.5 ml/L of humic acid each feed 3x 4 pints this week. These have a real funky smell to them, cant really put my finger on it, kinda plant musty, really strong smell, maybe skunky or something, main thing is they're getting the chop soon ! Clipped a fair few leaves but the idea is a dry trim and hanging the plants whole for 10-12 days of slow drying before the cure. Man i cant wait to wrap this grow up ! These kinda grew crowded, i can tell because the biotabs and mrB's have a few branches that are anemic, small, useless, so they're all gonna go into the bubble hash, let's see if i can extract anything from these beauties ! wish me luck 🚀

This is the start for week 9 and got to doing some last training and ended up splitting the middle stem, that end up with her got into shock unfortunately, she don't seem to understand happy but we will see

Defoliated a bit more, feeding sugar royal and green sensation. The smell is really coming through now.

It was a great nine week flower, there were no issues during growth and was solid all around. The Spliff genetics is great with there signature colas.. the plants took to the nutrients very well, I thought I was running a little high on my ppm on beginning of grower but it all leveled out and nothing burned the leaves or bugs. I used general hydroponics flora pro series, with fish sh!t all the way threw!!!

Fade colors are really starting to push through Fed with nutrients: 5/7/25 Fed with ph water at 6.5 : 5/11/25 Fed with nutrients 5/12/25

May, 8th Plant is still stretching, And Building some woonderful Flowers too A lot of WWork and Energy for the Plant I feed her in every Watering because shee is geting alittle Pale/ limy And I added some Calmag too, to help with Photosynthesis because she has still a big need In Nitrogen , added some flowering Nutrients, too

Day 70. Looking gorgeous. Day 74. Starting flush

Good morning 3 months ago I started four plants. 2 tropicozz 1 red red wine from perfect tree 1 mimofuel from ShadowFarm This is the first time I have tested cuttings. I struggled to root the cuttings but. we are happy today to have succeeded in 16 cuttings out of 20 I will start flowering in 1/2 week. In the coming days I will post other photos of the mother plants at the end of flowering and if I find them . kisses and see you soon Abi In Da HOoD / Uf042o