The Grow Awards 2026 🏆
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👉DIA : 13/12/21👈 Metodo de germinacion: 👉<< VASO DE AGUA >>👈 seleccionamos las cantidades deseadas de semillas y en un pequeño vaso con agua opmotizada insertamos las semillas para nutrirlas durante 12H, situada en un lugar sin luz, apropiadamente tapada con un trapo.😉 __________________________________________________________________________________________________________________________________________________________________________________ 👉DIA : 14/12/2021👈 👇👇👇👇👇👇👇👇👇👇👇👇 una vez pasada las 12 H de germinacion en vaso de agua , depositamos las semillas bien colocadas en un recipiente con papel de cocina humedecido con agua y 1ML de estimulador de raices (TOPCROP). Esperamos de 1 a 2 días a que salga la raiz de la semilla y ser transportada a los Jiffy😉 ___________________________________________________________________________________________________________________________________________________________________________________ 👉DIA 17/12/2021👈 👇👇👇👇👇👇👇👇👇👇👇👇 Pues pasados los 3 dias de germinacion en el metodo de papel, vemos que ya salieron a luz las raices y con sorpresa😱 NUNCA habia visto una cosa asin!! An salido GEMELAS, sii sii !!😵 de una misma semilla an salido dos raices una cosa impresinante! y damos paso a colocarlas en los Jiffy. 👉Instrucciones:👈 - Colocación de los jiffy en un recipiente con 2L de agua y 2ML de enraizantes de top Crop. - Dejamos actuar durantes varios minutos hasta que el jiffy se extienda. - Abrimos un pequeño orificio en los Jiffy para introducir la semilla con el cáliz dejandolo hacia afuera. - Colocamos en un pequeño invernadero para que tengan la suficiente humedad. - Ayudandonos de unos foco de pocos waltios, le damos a luz necesaria para esta face de germinacion dejandolas 24 horas de luz. ________________________________________________________________________________________________________________________________________________________________ DIA 22/12/2021 Colocacion de Jiffy en Maceta de 3,25L 👇👇👇👇👇👇👇👇👇👇👇👇 👉Instrucciones👈 -Pasados varios dias vemos que el periantio(semilla) ya se rompió y empezo a salir los cotiledones con una hermosa raiz blanca por debajo del jiffy. -rellenamos macetas de 3,25L con sustrato de top crop(anterior cultivo). -introducimos los jiffy en las macetas dejando la plantula bien colocada. -regamos con 3L agua opmotizada y 1ML/L enraizante Voodoo ADVANCED NUTRIENTS - Permanecemos con las 24 horas de luz con foco led. _________________________________________________________________________________________________________________________________________________ 👉DIA 25/12/2021👈 👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇 pasados 3 dias desde que se plantó en maceta. A dado unas respuestas increibles !!👍 las volvemos a regar y a cambiar las horas de luz que se pasarian a 18H
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Wrap up of the Award Day at Spannabis 2025 with Adam iLL!
That’s a wrap on an epic awards day at Spannabis! Congrats again to all our growdiaries.com community-voted winners for shining in the growing world! Thanks to everyone who joined us! #Growdiaries #Awards
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@jojopfoh
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A very easy strain to grow. She takes a bit longer to start flower due to the 85 day flowering stage. Watch bud density and make sure they get lots of air as they get very thick
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Another week in veg.. she's patiently waiting her turn to get into the other tent where she can start producing fruits ... i'm hoping for a good one with this and I have a feeling it will be.. Thanks again to @Grow4releaf... God bless and happy growing ✌️
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02 Feb 2022 Update: I noticed a strange chromatic coloring on some leaves of the "older" plants, a couple of the smaller plants have small brown spots on the leaves ... i also noticed that the grow tent is quite hot and the ground is quite dry, could be that the watering maybe is not enough, so from today i'll give a bit more water. I didn't expect that kind of temp from a led, adjusting the watering should goes better. Light regime ☀️21/3🌑 temp ☀️22/32 🌑 18.5/22 humidity 48% The lamp is till running it at 100% of his power but I just moved the lamp up to 70cm from the ground for more air flow and maybe the leds where to near the leafs, this why the chromed coloring on leafs? 03 Feb 2022 Update: Light regime ☀️21/3🌑 temp ☀️24/28 🌑 18.5/24 Plants looks already more happy now!!! soon photo/video update 04 Feb2022 Update: Little check and evrething looks great now, the new distance of the lamp did the magic , temperature is also much much better and stable, all plants look bit happyer now 😁 Light regime ☀️21/3🌑 temp ☀️22/26 🌑 20/18 humidity 51% 07 Feb 2022 Update: With a Lamp distance of 70cm at 100% is going really good, temperature is right and the earth in the pots also stays moist longer, the new leaves on all plants are doing well and the two "older" plants are doing a lot of side arms. In the last couple of days I have been noticing an increase in the speed of growth. The little BlackBerry Banana is staying small but is making new leaves that look healthier than the plant was born, I wait another week before deciding what to do but most likely I will pull her out of the tent and she will grow as an outdoor plat looking out the window 😂 Light regime ☀️21/3🌑 temp ☀️22/26.9 🌑 19.5/22 humidity 59% 08 Feb 2022 Update: After a little thinking i decided and Ijust moved the small BlackBerry Banana plant out the tent for a "Free" grow ☃️🌈🌤️ 😅😅 More air/co2 space and lamp ☀️ for the other 8 plants. Sometimes have to do wha need to be done.
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This week I am going through and pulling the large fan leaves off , she is a slow flowering plant so I'm just doing my best to be patient with her !!! A true gem with an unique structure !!! I think this girl is gonna be a 10 week flower , she is just starting to get smaller flower spots . Still a beast !! Added a CO2 bag directly above the canopy , added a 150 watt "blurple" light above GG#4 S1( number 2) More picture notrs and videos to follow !!
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Starting to focus on growing buds now, looking so good starting to smell strong too, few more weeks left to make these buds as fat as possible, how good does it look in the video 🤤🤤🤤🤤
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These one was a big messy jorney, everything happened from almost kinling them, to almost no space for them to grow, LED broken middle flower and still perform like the queen champion she is!!! I am felling her mature over cycles , i am thinking of her like a wine vine that gets better over time , these one in my humble opinion its a must in everyones garden <3 <3 <3 Wen harvesting these one i felt the need to break it all apart to prevent bud raw or anything we do not want on our medicine, i like to hang them all and let them dry but in these case there was no chance i was taking that big of a risk with these big fruits all over the place, what a blessed run, my rack is completely full, there was no more space , i mean no more space at all hahah amazing , thank you thank you thank you <3 <3 <3 As always thank you guys for your love , your time, your support and it all, i fell blessed and i am truly thankful <3 <3 <3 All info and full product details can be find in can find @

 https://www.zamnesia.com
 https://aptus-holland.com 

#aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #zamnesiaseeds #growerslove

With true love comes happiness <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 

 More info and updates @ 
https://growdiaries.com/grower/dogdoctor

 https://instagram.com/dogdoctorofficial

 https://youtube.com/channel/UCR7ta4DKLFMg2xxTMr2cpIg

 <3 <3 <3 Growers love to you all <3 <3 <3
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1/11: Fed today, but had no time for photos. 1/15: Didn't have time to feed today...😕 1/16: Fed everybody...little bit of drought...sorry girls... Uploaded photos- 😍 I put a new 15.0 intensity reptile UVB into the tiny tent, about 14-18" away from the plants. Hopefully that stresses them enough to make Wicked_Stix jealous of all the frost they produce....
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@Unpluggy
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Hi there, She is doing good so far. Topped her today at day 12. Exited to see if my pheno will be a purple one :) Have a nice day! Adding Progress pictures the next days.
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143gr for the Larger pheno and 97 for the shorter, very respectable yield in total for 3 Wk Veg. Total 240gr even they each had 1.5’x1.5’ of space. Big yielder, buds are 10/10 & very easy to grow . Could be prone to mold in an uncontrolled environment.
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@lino06000
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09/08/22 1 er jour 2eme semaine jusque la tout va bien! j'ai entamer le programme de nutriment de bio biz les pots en tissus me plaise énormément. 12/08/22 elle s'adapte très bien, je suis le processus bio bizz a la lettre pour le moment je lui donne 500ml a 800ml par arrosage. 16/08/22 bonjour dernier jours de la semaine qui c'est très bien passer r.a.s ,mademoiselle mesure 9 cm et bien portante
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@Kushizlez
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Day 40-47 (May 31st- June 7th) (Day 41) For the first time in this grow these plants are looking halfway decent. Smell is ramping up too. I finally feel optimistic about this grow. (Day 42) The two jacks and the chunky blueberry are showing early signs of N/Mg deficiencies. I know it’s late in flower but since I’m experimenting with this tent, I’m going to give them one final feeding of nature’s pride 2-5-5.5 (tbsp) along with a teaspoon of oyster shell flour and some rock dust. Everything else I’m going to give a teaspoon of rock dust and oyster shell. There is no way these amendments will fully break down in time to be used by the plant but they could at least use the pH buffering and trace amounts of minerals after their recent leach. Everything but the BAOGC is covered in pistils that don’t really match their bud size. I hope they fill into their pistils because an over abundance of hairs drops the bag appeal like crazy. Over the last few cycles, indoor and outdoor, I’ve definitely learned that different strains and phenotypes mature at different times. Sometimes really radically too. (Day 43) None of the plants are fading at all but they’re still looking good. Black garlic I know should be fading but remains dark green and toxed. I popped a developing seed out of the main bud too. I wonder if that blueberry herm branch pollinated it at all. It seems like plants don’t really like that Destiny dark matter soil by itself. I find it works better mixed with coco or promix 1:1. (Day 44) I’m starting to think I might be mixing up the TWOG and BAOGC. BAOGC took forever to fatten up last round and the 2 phenos I suspected already look done. I was thrown off by the similar terps. The beauty phenos are definitely TWOG (Day 45) God these Jack Herer’s smell good. Nothing but fruity funk. Almost like a vanilla or blueberry yogurt. I would say this stuff has the most overpowering smell in the tent now. I’ve noticed they’re starting to foxtail like crazy. Blueberry#1 too. I’m watering a final low strength feeding of seaweed extract on everything. (Day 47) Everything really fattened up this week. Even at the beginning of the week I was saying I didn’t think they would fill out. Jack Herer and BAOGC specifically made some big gains and nearly doubled their density and still have quite a way to go on them too! The smell has ramped up and finally, everything is looking happy and healthy.
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07 März to be updated. Ready for Chop? 09.03. Day 120 flower 57. Ich werde bald Ernten, denn ich bin beinahe komplett mit den Trichs zufrieden . Hellbraun+ glasige+ milchige. Nur noch zu wenig milchige. ? Frische Phylaxien ab und an überall. = Wachstum 🤗💚 NEHME ICH. 10. März Ab in die Dunkelheit für 3 Tage. Damit es so richtig scheppert. Als wenn sie nicht gut gelungen und damit auch stark genug wäre. Flower day 57. Eigentlich noch 13 Tage, aber danke ich bin der Meinung, unterstützt durch die Community (😙 ) das es jetzt gut ist. Also Ernte am Tag 60 eigentlich. Bisher 10 Punkte aufgrund von betörender Erscheinung und Geruch. Sehr feste Knospen. Der Rauch steht noch aus. 37 % THC AUWEIA ( Like you are an Snowboarder and looking to the snow avalanche that soon arrives you ) 12.03. morgen wird geerntet. Die einzige Pflanze bisher überhaupt, die ich noch nicht angetestet habe. 😬🌊🤗📥. Ja, mit Bildern. 13.03. Day 61 of flower. CHOP , ERNTE, AUS DIE MAUS, ZAHLTAG, Hängt Kopfüber zum Trocknen am Stamm. Die kleinste Knospe unten wird gerade getrocknet, und an getestet. Sie fühlte sich sehr klebrig an. Später gibt's den ersten Eindruck. ....... Eine Popcornknospe später: Ich bin so fertig. Sofortiger Einschlag, noch vor dem Auspusten. Geschmack geil. Nochmal, das war ganz wenig. Hat mich beinahe umgehauen. 37% ? Keine Ahnung. Kann aber sein. Definitiv das heftigste Weed was ich kenne. Hunger habe ich auch bekommen. Nochmal, kleinste Menge, heftigster Einschlag. 👍 Dankeschön. 14.03. Es ist vollbracht, heftigstes Weed Ever trocknet in meinem Zelt. Bin ich Happy? Ja. Und doch. 🌊📥💜 😬😬 HEFTIG 1 komplette Stunde war ich heftig von nem Krümel. Dann ging's besser. Interessanterweise war das aber komplett angenehm. Nun ja, wie bereits angekündigt wird es dann noch einen anderen Test geben. Mit ner " normalen " Menge. 😬 ( Ok , vielleicht erstmal doch zahmer, denn es ist so stark wie beschrieben ) Ich hoffe ihr erkennt dieses Glitzern. Im Sonnenlicht ist das so schön... 15.03. FUTURE 1 : Noch nie hatte ich soviel Respekt vor ner Ganja Pflanze. Die hängt im Zelt und trocknet. Aber doch, das war, dafür das das so wenig war...., EXTREM ! Und das war nicht mein erster an diesem Tag. ( 😂😂 Echt nicht ). Meine Empfehlung für : Anfänger nicht. Normalos nicht. Grower mit Toleranz: Vorsicht extrem Stark. Grower mit heftigster Toleranz : Genau Richtig. Wohl bemerkt , bei Knospen. Gutes Haschisch hatte schon vor 50 Jahren über 55 % THC. Aber dieses ANESIA Gebräu kommt dem echt nah. Was ein Einschlag. Es berührt den Gaumen, dein Körper sagt: Her mit dem Qualm Und genau ab da wirst du stoned. Sofort und ohne Umschweife. HIER ENTLANG MEINE DAMEN UND HERREN, ICH ZEIGE IHNEN FUTURE 1. Während des Ausatmens genieße ich wohlige Anklänge von Weihrauch, Haschisch, Floral und noch so ein paar Düfte die ich nicht kenne. Der Qualm ist draussen, ich bin bedient. Sehr angenehm, aber stark. Von dem Fitzel Weed rede ich. Ich konnte gestern danach nicht mehr schreiben. 1 Stunde lang volles Programm, dann ging Sitzen und gamen wieder. Danach brauchte ich ne Erholung. Ich. Ich war so fertig. Kein Weed knallte mehr richtig Wie ich geschlafen habe? Sehr gut. Mit vollem Magen. HEFTIGSTES 16.03. 😀😃😄😁 17.03. fühlt sich an wie Gelee. 19.03. die Knospen sind unglaublich harzig. Und lecker. Kratzt 0 Prellt 1000. ZEHN PUNKTE! Ist so. ANESIA SEEDS eure FUTURE 1 ist in meinen Augen ( nein kein Geschleime ) so schön. Dann dieser Geschmack, bisher noch recht frische Knospen, ist wie ein würziges frisches zusammenspiel von sour Creme mit irgendwas scharfen, welcher mit Ananas und floralen Akzenten, Pfefferminze, abgerundet wird. Untermalt von Limetten zitronigen. Die Mischung ist echt geil wenn sie Zusammenspielt. Vor allem, das schmeckt nach Weed! So , die Wirkung: Erstmal, egal was du den ganzen Tag so gemacht hast, danach geht nix mehr. Du merkst sofort, wie dein Körper runterfährt. Biing, wie ein sanfter, doch gut hörbrer, klingen einer Klangschale, wird dein Geist Stoned.. Das dauert ca 7 sec Angenehm, ohne Fahrstuhl Effekt oder sonstige Unannehmlichkeiten. So, du Rauchst, und bist sofort dicht. Sofort!. Die fragt nicht, die macht einfach. ( Immer noch ne Popcorn Knospe) 😳 Das sind die schwächeren. Ich kann mir gar nicht ausmalen ( doch kann ich 😬😬 ) wie die Oberen knallen. Heftig
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
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Note: Little chem-burn this week. Feed just water for 4 days with correct PH and they jumped back. Was not that bad though. Day 21 on flower!
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Hi all! 🤗👊 Week 4 flower hugeee buds I love this genetics my second times and 2 times he have nice buds In coco coir with perlite 80/20 Systeme auto pot 7gallons Remo nutrients