|»» LOG DISCLAIMER: Not intended as a grow guide. Grow log entries are usually posted 3 weeks later and are for my learning reference. ««| Introduction - Week 26 Summary - Ensign's log, stardate 17608.3, Wow what a week! Drying is always pretty simple and been checking daily. Buds are drying nicely and terps are smelling pretty dank!! Most of the week has been in the veg tent with tissue culture! Mixed another batch of establishment media and took proper explant cuttings. Also super-cropped the mothers to even out the canopy. Ahh yea! Getting hyped for trim day next week! Next week will also be the last week for this grow log, but am already planning out the next cycle. Anyways! Onwards to next week! [START OF WEEK 26] ========================================================== ---------- [ 4x4 MAIN TENT ] ---------- Heady OG BX1 #1 [Round4 ~Wk19] - WEEK 26 - Dry Wk1 ----------- [ 3x3 VEG TENT ] ------------ TKO - WEEK 09 - Full Veg Gorilla Goat #1 - WEEK 05 - Full Veg Heady OG BX1 - WEEK 09 - Full Veg Garlic Budder OG#1 - WEEK 05 - Full Veg 10/13/25 → 10/19/25 This Week's Estimated Goals ---------------------------------------------------------------------------------------------- DLI: ~0 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.5 kPa This Week's Meter Readings & Averages ---------------------------------------------------------------------------------------------- Light On/Off: 00:00 - 00:00 | Wattage: ~000.0W [LV00] Distance From Canopy: ~00” DLI: ~0.00 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.17 kPa Soil %: [47.4, 34.1, 35.0, 55.7, 43.7, 43.6, 44.0, 43.3] ========================================================== Day 01 - Monday - 10/13/25 - Total Days: 183 ----------------------------------- Daily Averages ~[+01:00] - Temps: 78.8°F [26.0°C] - Humidity: 64.4% - VPD: 1.19 kPa - Soil %: [49, 34, 35, 57, 44, 44, 44, 42] ----------------------------------- - [22:05]: Night check on the 4x4 tent. --- Ca-Caw!! Looking great! --- Heady OG BX1 #1 is drying along and no signs of mold. Also airflow and RH is stable and within the 60-70% range. --- Getting stoked! Trim day is 9 days away! - [22:09]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 48% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 40% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 40% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [22:18]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- All of the vegging mothers are looking great and the canopy is starting to even out. --- Also clones look healthy and no need to water yet. --- The stems, err, I mean, explants, don’t show any signs of contamination. --- Staying positive, glad the growth media is sterile and the overall procedure was successful. --- Anyways! Plan to create more establishment media and cut proper explants soon! - [22:21]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 02 - Tuesday - 10/14/25 - Total Days: 184 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.9°F [24.9°C] - Humidity: 64.8% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 43, 44, 43] ----------------------------------- - [21:44]: Night check on the 4x4 tent. --- Looking good in the dry tent! --- Heady OG is drying along and humidity levels look great! --- Nothing to do but maintain and wait to trim in 8 days! - [21:55]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 41% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 46% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [21:59]: Checked the 3x3 veg tent. --- Looking healthy and growth is raging! --- All mothers are looking beautiful! --- Clones are getting taller and looking healthy! --- Gorilla Goat #2 needs watering. Let’s go Ensign! - [22:03]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 4 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 230.8g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [22:23]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.7g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. --- Decided to train and super-crop both clones. --- Bent the taller branches to lower the canopy. - [22:38]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Onwards Ensign! Day 03 - Wednesday - 10/15/25 - Total Days: 185 ----------------------------------- Daily Averages ~[+01:00] - Temps: 75.6°F [24.2°C] - Humidity: 63.8% - VPD: 1.09 kPa - Soil %: [48, 34, 35, 56, 44, 43, 44, 42] ----------------------------------- - [+01:33]: Quick night check on the 4x4 tent. --- Looking great in the dry tent! --- Also starting to smell dank! --- 7 more days until the big trim! - [+01:37]: Checked the 3x3 veg tent. --- Veg garden is healthy and growth is green! --- Both the Heady OG BX1 #1 and TKO mothers will need another topping soon. --- Clones in the propagation tray also look great and no need to water. --- The explant media is still looking sterile with no signs of contamination. --- Easy night in the garden. Nice! - [+01:40]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Okay! Tomorrow plan to dive back into tissue culture and prep more growth media. --- Onwards Ensign! Day 04 - Thursday - 10/16/25 - Total Days: 186 ----------------------------------- Daily Averages ~[+01:00] - Temps: 74.6°F [23.7°C] - Humidity: 62.2% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 44, 44, 45] ----------------------------------- - [11:41]: Alright! Back to tissue culture and time to start another batch of establishment media! --- Took the plant growth regulators out of cold-storage (my fridge). ------ [ » SMOKES A BOWL « ] --- While the products warmed up, gathered the necessary supplies and setup my workstations. --- Currently, focusing on: ------ PHASE 01 » ESTABLISHMENT --------- » DAY 01 » MEDIA - PART 01 - Mixing the growth media --------- » DAY 01 » MEDIA - PART 02 - Sterilization of media and tools [via autoclaving] --- Going to use the same establishment formula, but doubling the yield. --- Also using RO water instead of distilled water. --- Here's the establishment media formula: --- [» VER.0001 |» INITIAL TROPICAL PLANTS FORMULA |» 0.5L (500mL) RO WATER «] ------ MS [Muashige & Skoog] --------- » 2.22g ------ Sucrose [Cane Sugar] --------- » 15g ------ BAP [6-Benzlaminopurine | Cytokinin] --------- » 0.5mg » 0.5mL » 500μL ------ IBA [Indole-3-Butyric Acid | Auxin] --------- » 0.05mg » 0.05mL » 50μL ------ AGAR [Jelling Agent] --------- » 3.5g ------ PH [Potential Hydrogen] --------- » 5.7 --- [» 1mg/mL CONCENTRATION ratio | 1mg = 1mL | 1mL = 1000μL «] - [12:59]: Time to prep the growth media! --- Made sure to turn OFF the lung-room's oscillating fan. --- Filled a measuring cup with 400mL of RO water. --- Dropped a clean magnetic mixing bar into the measuring cup. - [13:05]: Placed the measuring cup on the magnetic stirrer. --- Turned the unit ON and have the unit on LOW. - [13:11]: Using a precision scale (sensitive to 0.01g) along with a micro-pipette (1000μL capacity along with tips) and disposable (100mL) weigh boats, ------ Added 2.22g of MS [Muashige & Skoog] ------ Added 15g of Sucrose [Cane Sugar] ------ Added 500μL of BAP [6-Benzlaminopurine | Cytokinin] ------ Added 50μL of IBA [Indole-3-Butyric Acid | Auxin] ------ Added 3.5g of AGAR [Jelling Agent] ------ Then filled the measuring cup to the 500mL line before covering the top with plastic wrap. --- Alright! - [13:46]: Let the media mix on the magnetic stirrer for a bit. --- Have the unit set to LOW with a steady vortex. ------ [ » ~20 MINUTES LATER « ] - [14:09]: Okay! Time to pH the establishment media! --- Gently removed the bottom cap of the AC Infinity PH Meter PRO [AC-PHM7]. Placed the cap in the storage case for later. Covered the top with plastic wrap (KCL solution still inside the cap). --- Swirled the probe in a cup of RO water to rinse off the storage solution. --- Shook the excess water out and wiped the unit with a clean paper towel making sure all mineral buildup was removed (kept away from the very tip of the probe). --- Temporarily placed the meter back into the cup of RO water. --- Removed the plastic wrap of the establishment media. - [14:13]: Using the AC Infinity PH Meter PRO [AC-PHM7], --- Took the establishment media's initial pH reading. --- The pH was at 5.14 --- Using the micro pipette, ------ Added pH Up solution. --- Made sure to let the media solution mix for a bit before checking for pH readings. - [14:21]: Adjusted the media's pH until it stabilized to 5.58 --- For this batch, tried to go for a range of 5.4 to 5.8 pH. --- Also glad that I did not need to add PH down. - [14:43]: Poured the pH'd establishment media into separate polypropylene containers. --- Made sure to re-mix the media solution between container fillings. --- Ended up splitting the media solution into 12 containers. ------ 8x » 125mL Polypropylene Bottles [with screw cap lids] ------ 4x » 236mL (0.5pt) Glass Mason Jars [with polypropylene jamming lids] --- Have the lids of all vessels loosely on and set each aside for later autoclaving. --- Also filled 12 empty polypropylene bottles with RO water and placed the lids on loosely (4 bottles are re-used). - [14:58]: Prepped the tools for sterilization. --- Took a new 5.25in x 10in sterilization pouch and added the following tools: ------ 3x stainless steel forceps ------ 3x stainless steel scissors --- Before placing into the pouch, wiped each tool with a paper towel sprayed with 70% isopropyl alcohol. --- Sealed the bag and marked the date. --- Time to autoclave everything in the pressure cooker! --- Filled a Presto 23qt-Pressure Cooker with 12 cups of RO water. - [15:06]: Added a few aluminum foil balls to the bottom of the pressure cooker. --- Placed the first rack on top of the foil. --- Made sure the rack was level and stable resting above the water. --- Gently placed the containers of media into the pressure cooker and onto the first rack. Lids are still loose. --- Made sure to give space for each container and kept them away from the sides of the pressure cooker. --- Placed a second pressure cooker rack on top of the containers of media. --- Placed the containers of RO water onto the second rack. Lids are still loose. --- Placed the third rack on top of the containers of water. - [15:16]: Lastly, placed the sterilization bag of tools on top of the third rack. --- Took the pressure cooker lid and made sure the release vent was clear of debris before placing it on. --- Sealed and secured the lid. --- Pressure vessel has been sealed! --- Alright! Time to sterilize! --- While keeping the vessel level and straight up, moved the sealed pressure cooker to the kitchen for heating. - [15:23]: Placed the pressure cooker onto a butane-gas stove. --- Turned the stove ON and set it to HIGH. ------ [ » ~20 MINUTES LATER « ] --- Once steam was venting out of the release vent, --- Placed the metal rocker onto the vent. --- Shortly afterwards, the pressure top-lock sprung up to indicate the vessel was under pressure. --- Then the pressure gauge started to rise. ------ [ » ~10 MINUTES LATER « ] --- At 15psi, the metal rocker started to rock! - [15:56]: Turned down the stove down to LOW. --- Set a timer for 15 minutes and began monitoring the pressure gauge. --- Maintained the 15psi by making minor adjustments to the stove. ------ [ » ~15 MINUTES LATER « ] - [16:12]: Turned the stove completely OFF and waited until the pressure cooker's top-lock dropped down which indicated that pressure levels were safe to open. ------ [ » ~40 MINUTES LATER « ] --- Once the pressure cooker was at safe levels (0psi) and the top-lock was down, - [16:48]: Moved the pressure cooker into the lung room. --- The pressure cooker was still quite hot so was careful to keep the vessel straight up and level during transport. --- Used towels for insulation. --- Rested the hot pressure cooker onto a stack of 5-gallon buckets covered with layers of towels and folded paper bags. --- Gently opened the pressure cooker and set the lid aside. - [16:57]: While wearing nylon gloves, sprayed my hands with 70% isopropyl alcohol. --- Gently removed the bag of sterile tools placed it onto a Pyrex dish. --- Removed the top rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle of sterile water before placing onto the Pyrex dish. --- Removed the middle rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle and jar of sterile media before placing onto the Pyrex dish. --- Throughout the entire process, kept the gloves on and sprayed my hands regularly with 70% isopropyl alcohol. --- Decided to store all the bottles in the Pyrex dish. - [17:16]: Have the dish resting on a shelf in the lung room. The jars of media are set right next to the Pyrex dish on a towel. --- To prevent dust, covered everything with towels. --- Done for now and will check on the growth media tomorrow. --- If all goes well, should be taking new explant cuttings in a couple days! - [17:39]: Night check on the 3x3 veg tent. --- Looking beautiful and healthy in the veg garden! --- All moms are getting bushy and getting pretty close to the grow light. --- Since I plan to take explant cuttings soon, not going to top or train quite yet. --- Clones also looking healthy and seem unaffected by the super-cropping. Nice! - [17:57]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 45% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 44% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [17:59]: Checked the 4x4 main tent. --- Ca-Caw!! I got Headies! --- Wow! Heady OG BX1 #1 is drying along beautifully and she is smelling dank! --- Also no signs of mold and RH levels look stable! --- 6 more days until the big trim! - [18:11]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Still gots more to do! --- Let’s go! Let’s grow! --- Onwards Ensign! Day 05 - Friday - 10/17/25 - Total Days: 187 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.0°F [24.4°C] - Humidity: 62.0% - VPD: 1.16 kPa - Soil %: [48, 34, 35, 55, 44, 44, 44, 42] ----------------------------------- - [22:57]: Night check on the 4x4 tent. --- Ca-Caw! Headies is smelling dank! --- RH levels are getting a bit dry, but are okay. --- Wow buds are frosty with trichomes! --- With 5 days until trim day, getting stoked! - [23:04]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 47% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 45% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 50% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [23:11]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- Mothers are getting bushy and tall! --- Super-cropped and trained the taller branches down to lower the canopy. --- Plan to take tissue culture cuttings tomorrow so glad there are plenty of tops to choose. Still deciding on which mother to preserve next. --- Clones in the propagation tray look healthy! --- The original Garlic Budder #2 clone needs watering. - [23:26]: Here is the starting initial watering range: ------ Garlic Budder OG#2 » 256.4g → 300.2g --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Garlic Budder OG#2 » 258.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [23:40]: Took the saturated weight after watering, ------ Garlic Budder OG#2 » [000.0]g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [23:45]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 06 - Saturday - 10/18/25 - Total Days: 188 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.8°F [24.9°C] - Humidity: 60.3% - VPD: 1.25 kPa - Soil %: [47, 34, 35, 55, 44, 44, 44, 47] ----------------------------------- - [15:19]: Decided to decrease the VPD level a bit. --- Within the AC Infinity App, --- Changed the schedule name from 'Flower [12/12]' to 'Drying [0/24].' Oops. --- For the Main Tent [4x4] controller, --- Adjusted the 'Drying [0/24]' Schedule for the exhaust fan. ------ Decreased the VPD LOW trigger value from 1.5kPa to 1.4kPa --- Adjusted the 'Drying [0/24]' Schedule for the humidifier. ------ Decreased the VPD target value from 1.5kPa to 1.4kPa --- This should increase the relative humidity a bit and will maintain a 60-65% range - [16:59]: Evening check on the 3x3 veg tent. --- Wow the veg garden is raging with healthy growth! --- Even with the lower veg temps, growth has been perky and bushy! --- The TKO mother has been looking a bit on the purple-side, but I plan to increase the temps back after trim day. - [17:23]: Okay! Time to continue the tissue culture journey! --- Checked on the sterilized media, water, and tools. --- Ooo yea! The growth media in the glass jars have solidified nicely! --- The media in the bottles also look great! Found only one that did not gel properly. --- Hmm. The sterile water bottles are not looking sterile. Unlike the first batch (using distilled water), each RO bottle had tiny white particles floating in the water. --- I think this was due to the water source. The RO water used was from the plant reservoir. For the next sterilization, will try using water straight from the tap. Will discard the entire batch and try again later. - [17:34]: Anyways! Time to cut and sterilize another batch of explant cuttings. --- Using the similar protocols as last time, but with a few changes. --- Instead of distilled, using reverse-osmosis (RO) water. --- Also simplifying the workflow and will not be using the sterile water. --- Only taking cuttings from one cultivar. --- Today, attempting to preserve Heady OG BX1 #1 --- Unlike the last attempt, going to be cutting apical nodes and will leave all leaf growth on. --- Last attempt was more for monsteras and not cannabis. - [17:35]: Made sure the lung room's oscillating fan was OFF during the entire process. --- Put on a fresh pair of nitrile gloves. --- Okay! Let's grow Ensign! - [17:47]: Filled a glass beaker with 180mL of RO water. --- Using a micro-pipette, ------ Added 20mL of Clorox bleach --- Mixed the solution. - [17:52]: Poured the bleach solution into a glass mason jar. ------ Added 1 drop of Dawn dish soap. --- Dropped a magnetic mixing rod into the jar before setting it onto the magnetic stirrer. --- Turned the unit ON and set it to a low setting. --- Placed a polypropylene lid loosely on. - [18:02]: Rinsed and filled the glass beaker with 70% isopropyl alcohol. --- Filled another glass beaker with RO water before adding a drop of Dawn dish soap. Mixed the solution. --- Okay! Time to take the cuttings! --- Using a clean pair of trimmers, - [18:09]: Gently cut two clone cuttings from the Heady OG BX1 #1 mother. --- Stripped each cutting down to 2-inch apical nodes making sure to leave about 0.5 inch of stem growth for planting. ------ Prepared a total of 2 nodal cuttings. --- Dropped the nodal cuttings into the cup of soapy water. --- Swirled the cuttings in the soapy water for a bit. --- Opened the jar containing the 10% bleach solution. --- Using clean tweezers, --- Took each cutting out of the RO water and carefully submerged it into the beaker of 70% isopropyl alcohol for about 30 seconds. --- Then transferred the cutting from the isopropyl alcohol straight into the jar of bleach solution. --- Once both cuttings were in the jar, sealed the top with the polypropylene lid. - [18:23]: Flipped and set the entire bleach jar upside down onto the magnetic stirrer. --- Started using the magnetic stirrer. --- But the cuttings were getting hit by the bar so ended up turning the unit OFF. --- Instead, made sure to swirled the bottle every few minutes to agitate the cuttings. --- For a proper sterilization, the cuttings should be agitated in the hypochlorite (bleach) solution for at least 20 minutes. --- While waiting for the explants to sterilize, prepped the still air box. - [18:39]: Unfolded and placed a Safloga still-air box onto my desk. --- Thoroughly misted the inside of the air box with 70% isopropyl alcohol making sure to coat the entire chamber. --- Then isopropyl misted and placed the following items inside of the still air box, ------ x1 » Jar of Establishment Media ------ x1 » Bottle of Establishment Media ------ x1 » Glass-Bead Sterilizer ------ x1 » Roll of Plastic Wrap [pre-cut] ------ x1 » Sterilization Bag Containing: --------- x3 » Stainless Steel Forceps --------- x3 » Stainless Steel Scissors --- Made sure to fully coat the outside of the tool bag and each of the containers with 70% alcohol. --- The bag had a torn corner (Oops), so made sure to spray the inside with a few mists of isopropyl alcohol. --- For the glass-bead sterilizer, ------ Wiped the entire unit with isopropyl alcohol. ------ Before filling the unit with the included beads, misted the heating chamber with a spray of isopropyl alcohol. ------ Misted the glass beads again with a spray of isopropyl alcohol. --- Set the cleaned glass-bead sterilizer into the still air box. - [19:02]: Still air box fully prepped for explants! --- Okay! Explant nodal sterilization complete! --- While still wearing gloves, --- Misted the outside of the explant jar with 70% isopropyl alcohol before placing it into the still-air box. --- Turned the glass-bead sterilizer ON. --- Made sure to re-seal the chamber. - [19:05]: Okay! Finally time to start the tissue culture transplant! --- Let's go Ensign! --- Sprayed my gloved hands with 70% isopropyl alcohol (also put on a pair of cloth arm sleeves), --- Placed my hands through the arm holes and into the still air box to begin the process. --- Started by laying out my initial workspace: ------ » Far Left Quadrant » Jar of Sterile Establishment Media ------ » Far Center » Bottle of Sterile Establishment Media ------ » Far Right Quadrant » Glass-Bead Sterilizer ------ » Mid Center » Bleach Jar with Explant Cuttings [Main Workspace] ------ » Near Left Quadrant » Empty [Trash Area] ------ » Near Right Quadrant » Bag of Sterile Tools & Roll of Plastic Wrap --- Opened the bag of tools and placed the forceps and scissors into the pre-heated glass-bead sterilizer. --- Set the bag in the trash area (near left quadrant). --- Loosened the lids of both containers of sterile media but did not remove the lids. The lids were resting on top and could easily be lifted. --- Also took lid off of the bleach jar and gently set it on the table. --- Took the jar of sterile media and set it in the center of my workspace (to the right of the bleach jar). --- Using my left hand, took a set of scissors from the glass-bead sterilizer and gently cut the bottom of the first cutting. --- Once the bottom cut was made, placed the scissors back into the glass-bead sterilizer. --- Using my left hand, lifted the lid off of the jar of sterile media and inserted the trimmed end of the cutting straight into the growth media (using my right hand). --- Covered the jar with the lid and placed the forceps back into the glass-bead sterilizer. --- Tighten the lid of the jar and set it aside. ------ [ » REPEATED THE ABOVE PROCESS FOR THE BOTTLE OF GROWTH MEDIA « ] --- Once the last cutting was transferred from the bleach jar into the sterile media, --- Turned the glass-bead sterilizer OFF. --- Cleaned up my workplace and placed the scrap stem tips and bleach jar out of the way (near left quadrant). - [19:28]: Wrapped the lid of each explant container with plastic wrap before setting it in the center of the workspace. --- Explant transplant completed! - [19:59]: Using a label printer (Brother DT410), made a set of labels for each container: ------ Heady OG BX1 #1 | 04.24.25 | Photo | Reg ------ Formula: v0001 | ExBatch: 0002 | Lot: 01 | Pod: 01 ------ Establishment Media | Sealed On: 10/18/25 --- Opened the still air box. --- Then one container at a time, took an explant out and wrapped the lid with a label. --- Set the labeled containers onto the propagation tray (in the 3x3 veg tent). - [20:07]: Done! ------ The explants are around 37 μmol/m²/s which gives a DLI of 2.40 mol/m²/d under an 18/6 light cycle. --- Will check daily for contamination. --- Ca-Caw!! Ahh yea! --- Heady OG BX1 is now officially my first true attempt at cannabis tissue culture! --- Using this new workflow, I may try reusing the TKO media. Do plan to take new cuttings soon. May even try using the liquid media. --- Shout out to microclone and Bill Graham! His 'Tissueponics' video contains tips and tricks of the trade that have straight up changed the way I think of DIY cannabis tissue culture. --- Plan to further refine my process and will continue to refine the workflow with each attempt. - [20:15]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 54% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 48% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 45% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:56]: Checked the 4x4 tent. --- Ca-Caw! Looking great my ladies! --- Humidity levels are still a bit on the dry side, but still okay. - [21:08]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Oh yea, 4-more days until trim day!! Ca-Caw! Let’s go! Day 07 - Sunday - 10/19/25 - Total Days: 189 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.7°F [24.8°C] - Humidity: 59.4% - VPD: 1.27 kPa - Soil %: [48, 35, 35, 55, 44, 43, 44, 42] ----------------------------------- - [07:08]: Quick morning checked on the 3x3 veg tent. --- The veg garden is healthy and perky! --- All moms and clones are healthy and raging with growth! --- Both Heady OG BX1 #1 explants also look alive and green! - [19:26]: Night check on the 4x4 tent. --- Ca-Caw! We got MENDO DOPE in the trunk! --- Wow terps are getting loud and buds are drying along nicely! --- Looking frosty and coated in trichomes! --- Three more days until trim day!! - [19:33]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 46% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 54% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 40% - [CH05 BL » GR7 - TKO] ------ 43% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 41% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [19:35]: Checked the 3x3 veg tent. --- Vegging along beautifully in the garden! --- Mothers are getting taller and probably could train, but will wait until tomorrow. --- Clones and explants also look healthy and green! --- Gorilla Goat #2 needs watering! - [19:44]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 5 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 244.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [20:04]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.6g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [21:41]: With a few days before the big trim, decided to sample a few buds! --- Cut and trimmed branch for sampling and wow she’s got terps! --- Buds are dense and have a gassy diesel funk! Ca-Caw!! Wow! --- Without even curing, pretty sure she’s the one! --- Plan to grow out another cycle (in clone of the same pheno), but I think I found my female for my first breeding project! - [22:01]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Ahh yea! Three more days until the big trim! --- Also next week will be the last for this log. --- But already stoked for the next! --- Planning on popping more seeds and continuing the pheno hunt for another female! --- Since I lost TKO this harvest cycle, plan to grow a clone cutting of the same pheno (#2). --- As for seeds, popping more regulars and going to attempt to grow some of Mr. Grow-It’s Chillout OG! --- Got a pack of F3s and am stoked to give them a try! --- Also going to sprout some beans of Dead Ops OG from Zaza Genetics! --- Chillout OG, Dead Ops OG, and another round of TKO! Let’s go! Let’s grow! --- Hunters of the dank! Ca-Caw! --- Anyways! --- Onwards to next week! [END OF WEEK 26]

In Woche 8 bei den BlueberryMuffins war viel los. Die Sanlight musste täglich neu auf die 40cm Abstand gehängt werden. Hier und da wurden immer mal wieder ein paar Blätter entfernt die Triebspitzen verdecken oder so dicht gewachsen waren das es zu einer schlechten Luftzirkulation gekommen wäre. Die zweite Lage vom Scrog-Netz wurde installiert. So können die gestreckten Triebe wieder etwas ordentlicher untergebracht werden und werden dazu etwas gestützt wenn es in die Blüte geht. An den Stangen vom unteren Scrog-Netz wurden noch zwei PC-Lüfter angebracht um unter dem Blätterdach für etwas Luftzirkulation zu sorgen. Den optimalen VPD zu halten war etwas schwierig bei dem Wetter zur Zeit. Die Temperaturen lagen um die 24°C und die Luftfeuchtigkeit um die 60%. Mit kurzweiligem Lüften des Raumes ging es immer mal wieder in die Richtung der angestrebten 50%. Da werden es die hoffentlich bald kälteren Tage wieder etwas angenehmer machen den VPD in den optimalen Bereich zu bekommen. _____________________________ Düngen : Gegossen wird mit Wasser das min. 24h mit einer Aquariumpumpe in einer 10L Gießkanne in Bewegung gehalten wird. Davon wird soviel genommen wie es die Ladys verlangen und mit dem einen oder anderen Zusatz versetzt. Je nach Stadium der der Ladys. Ab ungefähr Woche 5 wird mit 10% vom Topfvolumen gegossen. Das ganze wird in drei Teilen mit einem Abstand von 20-30min gegossen. Tag50 gab es für die BlueberryMuffins 1ml/L PowerRoots, 1ml/L PureZym und 1ml/L PowerBuds. Das ganze wurde auf einen ph-Wert von 6.5 gebracht. Es gab je Lady 1L in den Topf und 200ml in den Untersetzer. An Tag52 gab es 1ml/L PowerRoots, 1ml/L PowerBuds, 1ml/L PureZym, 1.5ml/L PK 5•8 und 2ml/L StickyFingers. Das ganze wurde auf einen ph-Wert von 6.5 gebracht. Jede Lady bekam 200ml in den Untersetzer und 1L in den Topf. Tag55 gab es 1ml/L PowerRoots, 1ml/L, 1ml/L PowerBuds, 2ml/L PK 5•8, 2ml/L StickyFingers und 1g/L BioEnhancer. Das ganze wurde auf einen ph-Wert von 6.5 gebracht Jede Lady bekam 1L in den Topf und 200ml in den Untersetzer. ________________________________ Höhe gemessen ab Substratoberfläche #1      62cm     #2      66cm #3      37cm     ______________________________ Licht Abstand:  40cm Watt:        100W (50%) Ppfd:         550 DLI:           24

12/05Gave the cover crop a light watering this morning to keep the bed happy. The girls are looking a little hungry gonna give light feed next watering probably tomorrow. Temps have dropped some due to winter weather 12/06 gave light feed with this watering and a little more water per plant now that roots are established.. IPM sprayed at lights out with lost coast plant Therapy gave a light spray with some wdg3000 to pots and about a gallon of it into the cover crop.

Flowering is developing well. Very resistant genetics to fertilizer overdose, if it accepts it it is because it needs it. Dosage to monitor depending on the evolution

23/04/2025 Lights On! Sprayed walls 6%HP/L sprayed Domes 0.05% HP/L Pulled backwards MS with tape to try and shape her. 23/04/2025 Lights Off! 25/03/2025 Lights On! Placed seedlings in the 5L starting cans. Lights Off! Check the VT me like the old me! 26/03/2025 Midday! 27/03/2025 Lights On! 28/03/2025 Lights Off! 30/03/2025 Lights On! Defolled! Switch to flower in 4days. I need to setup the sativa tent in loft! Midday! Increased light: 460watts they perked up and are praying! - "If the truth perished, it'll still stand true that the truth perished"

Stacking up beautiful tons of bud sites added cal-mag her tips are burning. Well check her again in the morning. Did some defoliation as well.

The struggle is real as we continue limping to the finish line... A few more feedings this week and then on to the flush. Struggles aside, she smells great and is packing on. Photos/video taken 119 days after breaking soil, day 42 of flower.

Esta semana, nuestras plantas empiezan a mostrar las primeras flores. Ya tenemos la sala con las cuatro luminarias funcionando al 75% de su potencia y las he separado un poco más, ya que veo que con led, el espacio internodal es muy reducido. Añadimos a la mezcla de fertilizantes CALMAG de BioBizz, que aportará un extra de calcio y magnesio. Regamos cada tres días aproximadamente, una vez con fertilizantes y otra vez con agua. Comenzamos a reducir la humedad relativa en la sala y desconectamos la calefacción en fase nocturna, para que empiecen a pasar un poco de frío.

Así es como empezamos la tercera semana,seguimos con humedad alta pero la vamos controlando.. Estos días da buen tiempo la humedad en la calle será mucho más baja así podré meter aire sin tanta humedad.. De momento todo bien en el cultivo ningún problema hasta la fecha. Ya podéis ver,y comentar sobre algo que veáis que yo no! Como dicen! Ven veinte ojos mejor que dos! 🖖 Día 12 Último fungicida prevención mutritis,hongos humedad. Primer riego foliar hormonas producción de flores. 🌹 Día 13 Nada en especial solo observar si veo algún cambio tras las hormonas,aún no veo grandes observaciones fuera de lo natural del paso de los días. En el día de mañana por lo que me recomendó el fabricante se verán los resultados a la vista! Comentar que seguimos con la iluminación al 100x100 de capacidad Marshydro sp3000 x2 Hero led Ignator t720 Yxo led 60w ir+un espectro completo En la siguiente semana me llega el nuevo driver de philips para poder conectar la lámpara de IR que pude adquirir a Yxo nuevamente! Lampara led espectro completo + IR 70w . Me vendrá muy bien para el pequeño espacio de la puerta que necesita más iluminación para poder desarrollar bien esas dos plantas,de la entrada. La humedad fue cosa del pasado. Evite el uso de desumificador eléctrico añadiendo varios de sales,deciros que funcionan muy bien no dan un control exacto pero mantienen una humedad interior estable para todo el periodo de floración! 50% de humedad lo demuestra! Hoy añadi un poco más de sustrato. 110 litros exactamente sobre la cama de cultivo que tenía de topcrop con humus de lombriz,tierra diatomeas y alimentada con npk. Después de eso tengo en mi cama de cultivo 810 litros de sustrato para mis 44haze. 💐 Saludos Día 14 Anulado el intractor,ya que el propio extractor mete aire fresco por las rendijas de ventilación.. En lo largo de la noche iré viendo el resultado en datos.. Ya coloque los soportes para la nueva iluminación que en estos días llega su driver de sustitución E visto que algunas plantas en estos dos días están en 67-70cm.. Ya colocado el sistema de riego para evitar más pisadas en el cultivo ya que algunas se realentizaron por pisar encima de sus raíces.. Le e metido una pequeña poda de bajos para airear más la zonas bajas del cultivo. Pequeño riego de folacion con remedio de agua azúcar para dos que estaban más estresadas.

Hi all! 👊😊 Another week started ohh yeaahhh I do foliar 3 times this week and i see pistil white change in orange Smell very good pine and citrus :)

Mientras yo me divertía en la spannabis las niñas se ponian cada vez mas resinosas farmers!! Solo basta ver la shining haze para darse cuenta que el mammoth p es brutal!!👌🏻

09/16: Fed 30/70 blend of Veg to Bloom to all the photos. Noticed PM on True OG. Didn't notice yet but my OBW (and some other autos) also had a bad PM infection. Light defoliation and trimming 09/21: More PM found. Again light defoliation and trimming. Aphids also found on some of the autos.

Switched them to flower on 7/5/18! On 7/14 I switched the nutes to the "transition to bloom" blend which basically means it's winging them off of vegetation nutrients. Next week (week 3 of flower), I will then switch to the full on bloom blend! Other than that, just keeping them trimmed and trained as they begin to flower!

Had a great week. These babies grow slowly but thoroughly. Definitely will be running more.

Both ladies are getting fat and heavy 1 more week for the Gorila Cookies and harvest and 2-3 weeks for the Gorilla Glue and it will be time for drying curign and smkoing 🙂

Day 84 Week 6 flowering

This week was great my girls are growing well and becoming very beautiful young ladies 😂. Starting to flower and I can’t wait for them to Become mature women 😂 I’ve definitely got a girl crush, I’d kiss a girl and I’d like it if I could kiss my plants 🌱! ❤️❤️❤️

Maturing fast. Some botrytis unfortunately . Have to dig in to incoming air filtration to limit spores. Ac infinity terraform just been released maybe it could help with more sterile air.

1/4: Moved a few other plants out of the closet, now they've got a little elbow room. Both MPP's are fattening and frosting up very nicely. They are so similar that they could be mistaken for clones. Very, very stable genetic.👍 1/7: Fed today and gave them the sniff test...delightfully sweet minty aroma! I added a dose of endoboost in their feed today...kinda worried about the monstrous size of my plants considering that they are only in 3 gallon pots..