.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Its been a good week they are smelling pretty strong already. Switched to early bloom feeding. They started to stretch pretty good this week and, were taking up water quicker than i realized so I topped the pots off with some extra soil and started to water heavier they are thirsty.

Been a great week, not much to say on the dynomyco as it's early stages but im sure to see major improvement over the next week! I will say at this early stage though I do have alot of stable new growth so shows something is working in them roots but as for my females now, wow it's already shown it's work there as I used it on transfer and mixed Into the medium of the rear females so I'm impressed being a first time user! I will say i have learned alot about it following ari and the firebudz team on Instagram, so if anyone needs any pointers check them out they run classes too! Next one is Wednesday at 5pm PST very very educational growmies! Peace and love and stay tuned for greatness 💪🏽💯👊🏽💚

Haven't touched much of anything, and the roots are starting to find their way out of the basket.

Nothing but good news here. I have faith in the ladies!

💩Holy Crap Growmies We Are Back💩 Code Name FBT2307 Well growmies we are at 56 days in and everything is going much better , she's bounced back pretty good👍 really good actually 👌 👉 Shes getting frosty now , and i hope she fills out 👌if she does gonna have some buds on a stick 💪 Lights being readjusted and chart updated .........👈 Even with early major issues due to the soil/medium she's come a long way 👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Mar 24: Watering till runoff now... runoff ppm was 2500+... Will be using very mild nutes to flush over 1 - 3 feeds... or until I am near my input. Mar 26: Continuing to use 650ppm feed with bloom focus, 2L per plant gives 15% runoff... Runoff ppm is still very high... I wanted to check pH of runoff but the color is so vibrant i cant use the pH drops to view color, need to get a pH probe. Plants are thriving, no issues at all. the extra fan fixed the post-water droop. One plant is bushy and has the most bud sites, One is the smallest but has the most uniform structure, this one has the biggest buds and thickest stalk, One is about in the middle of the 2. Mar 29: one of these girls gets droopy early when its time for water, thought the canopy was too high so i tied down some more, no cigar... gonna try increasing calmag, if that doesent work im going to reposition in the grow box to a side instead of middle, may not need the upper end ppfd...

At the end of last week, finally flower indicators started to show and these week it went more. Day 32: I sprayed them with Rhizotonic this week. This will be the last week for sprays. I don't like to spray my plants while having buds on it even thought it is organic. Always make sure you spray from below the leafs. They will uptake it better and also spray some above. With the lights off. Did some defoliation again and removed some lower internodes which will be crappy buds anyways. Made a video of my feeding bucket as well, in case some of you wonder how I feed all those plants manually. It is a pump with an gardenhose attached. Thats it for this week, Until next week!

Flowering day 43

Awesome stuff from these ladies. .. They are fully into their new soil at this point and I am suspending nutrients for this week. I think that the 707 w/Bio-Live should be doing the trick for them right now. They are growing rapidly and drinking TONS of water. I noticed a little yellowing toward the bottom of the sherbs and what appears to be a potassium deficiency. I mixed up some terp tea. 4 tsp grow and 3 tbsp bloom in 5 gallons of water. UPDATE: In two days the sherbs is STOKED again. The roots are clearly healthy for it to take those organic nutrients that fast. So far, this is BY FAR the easiest organic soil system I have used.

Can't wait to blazeeee Feeding💪 10/17 Water30L+Cleanse80ml+calmag@190ppm Ph6.3 Ebb 10.42/11.32/12.26/13 Clone 11.35/12.21/13.05/14 Average runoff ec1.5 Keeper 300ml per pot 10/18 Water26L+Flawless90ml+calmag@300ppm Ph6.3 Ebb 11.38/12.24/13.17 Average runoff ec1.2 ph6.5 Clone 12.38/13.56/15.20/16.10 Average runoff ec1.5 ppm750 10/19 Water26L+Cleanse80ml+calmag@200ppm Ph6.3 Ebb 11.12/11.48/12.24 Average runoff ec1.4 Clone 11.50/12.12/12.37/13.37/14.40 Average runoff ec1.3 ph6.6 10/20 Water30L+Cleanse30ml+Calmag@190ppm Ph6.4 Ebb11.42/12.22/13.20/14 Clone 12.40/13.20/14.20/14.45/15.15 Average runoff 1.2ec ph6.4-6.7 10/22 last light day 10/23 2 Pm Cut and hang Plan 21day at 23temp 55rh

These one is by far my favorite tester, not only i love them number 9 as i said before, but she is evolving in to a very , very beautiful plant covered in resin, i mean for her age the numbers are just out of the world. with the week evolving she keeps getting better and better and now she starts to smell also and i am felling some mint notes , but mb is just me, too soon so say whatever actually but one thing is for sure , this one will be AMAZING amazing amazing just from the looks now cut the caMAg boost , she does not need it anymore , she looks kind happy and great as she is so hihihihihi As always thank you all for stopping by, for the love and time you guys spend on my stuff, i fell blessed and humble, many thanks to you all <3<3<3 Genetics - Fast Buds Tester 2309 Ligth - LUMATEK ZEUS 465 COMPACT PRO 
Food - APTUS HOLLAND #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #fastbuds #dogdoctorofficial #growerslove With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 
All info and full product details can be find in can find @ https://2fast4buds.com/ wen released 

https://aptus-holland.com/
 
https://autopot.co.uk/ 

https://lumatek-lighting.com/ Have a bunch more diaries going on, fell free to drop by and say hi dont be shy <3 <3 <3 <3 <3 <3 Growers love to you all <3 <3 <3

Ohhh ragazzi che roba stupefacente!! Questa penso sia l ultima settimana forse neanche tutto solo 2 o 3 giorni e la ragazza è pronta x essere raccolta! Non mi aspettavo delle cime così giganti e piene di resina che sembra ghiaccio! L aroma è davvero qualcosa di indescrivibile! Sentori di ciliegia 🍒 e retrogusto tipico della cola,invadono totalmente la mia casa! Spero che i vicini non sentano l odore! Devi fare i complimenti alla luce di Marsh hydro che mi permette di raggiungere dei risultati così professionali! Ringrazio anche FastBuds per le sue meravigliose genetiche che mi hanno aperto un nuovo mondo di gusti spettacolari! Buon 420 a tutti!

Days 15 to 17, varying by plant: My journey of experimentation, aiming to refine and perfect my cannabis cultivation methods, continues. This venture’s ultimate objective is to position myself as a preeminent cannabis cultivator in my locale. As I navigate through the labyrinth of regulatory licensing, I’m conducting a trial run with my seedlings under continuous lighting conditions and a minimal nutrient regime. My primary goal during this phase is to identify any potential hiccups that could hinder the growth process and ultimately impact the productivity of my intended commercial operation. As I delve deeper into this exploratory phase, I draw invaluable insights from the thriving cannabis community, whose collective wisdom informs my decision-making process. Given my geographical location and available resources, I’ve opted for a strategic shift in my cultivation strategy. Previously, I’ve been procuring seeds at an average cost of $10 to $22 per seed, with a relatively broad success rate variance, ranging from 70% to 85%. However, an alternative presents itself in the form of local cultivators. These businesses offer cannabis plants aged between 2 to 4 weeks, boasting an impressive variety of over 100 strains. These juvenile plants are offered at a cost-effective rate: an average of $100 for eight plants. With a success rate hovering between 70% and 95%, these plants offer excellent value. Essentially, I can access pre-grown plants at a substantial discount, in turn significantly boosting my chances of success. Consequently, I am poised to transition away from growing cannabis from seeds to harvesting. By sourcing my plants from local cultivators, I stand to significantly reduce overhead and production time—an undeniably smart move from a business standpoint. This strategic pivot signifies the end of an era and the exciting beginning of a more efficient and cost-effective chapter in my cannabis cultivation journey. I am currently outlining a plan to expand my cultivation by acquiring several juvenile plants, which should be happening between July 1st and 3rd. The new additions to my garden will include Acapulco Gold, Gelato, White Truffle, and Runtz. These will be cultivated in harmony with the existing growth, which comprises God’s Gift, Northern Lights, and the final surviving F1 Epsilon. My primary objective is to establish the most efficient and cost-effective supply chain possible. I aim to achieve this through the use of an off-grid system that runs solely on solar power, which will ensure complete sustainability and renewability. From a business standpoint, this approach is advantageous as it allows me to leverage the growth of high-quality plants that are already in the cultivation phase. The new plants are expected to arrive in about a week. If the growth rate of the currently cultivated plants doesn’t meet expectations, I’ll be incorporating several of the newly arrived plants into the hydroponics systems. This methodical approach aims to ensure an optimal growth rate and the highest possible yield. I also wish to address the cannabis grower community directly. I hope that my decision to skip the seed-to-plant stage does not cause any offense. My intentions are simply focused on maximizing quality and output, while minimizing our carbon footprint. I believe this approach is in the best interest of sustainability and efficiency, without compromising on the quality that our community values so highly. As we proceed with this venture, we’re currently in the phase of collecting equipment and navigating through the licensing process. To help lower our overhead and assist with consulting fees, we would greatly appreciate any donations, especially in terms of lighting equipment. Your support in this endeavor will contribute significantly towards optimizing our operation and will be deeply appreciated. Disclaimer: Let it also be known I am completely abiding by all state laws, and do not plan on selling any of this current bud. This is for research to avoid future growing "potholes" in the growing part of the business as we have a very small team. My end goal is to help change the current stigma around cannabis and provide a useful service that can help localize and reduce the inflation on cannabis prices in socal by providing a logical supply system. Again I intend on doing all things through the legal route as there is no need to do other wise given the laws on personal use and growing.

Light schedule 20 hours on 4 hours off and the same with ventilation. Humidity approx 50 percent, water intake raised up 125 ml per plant per day. Fertilization continues on Monday, Wednesday, and Friday with Biobizz family and Tuesday, Thursday, Saturday bat guano and Epsom salt. This week will change that shit lamp and replace with Marshydro 600W. Day 46, 2 days no update all good they are flowering nicely waiting for the new lamp. Critical Orange Punch nicely developing and the rest of them is coming as well. Today I set the new 600W Marshydro. I also set the light schedule for 18/6.

Growing great, looks healthy. Amitabha!

tras problema del balastro dos crecen con algo de retraso pero bien estimuladas y una queda poco mas atrás y con crecimiento lento pero nuevas hojas salen bien de buen color y forma

Good week for the girls, I had to defoliate a bit, but nothung much. They are growing fast and well !

05/27/2024 : ambered trichromes in sight (on leaves, not so much on flowers yet) DLI tops between 40 and 50. Lot of colors from leaves and pistils and powerfull smell! Now in late flowering, plant is maturing nicely.