2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Almost ready to start feeding nutrients noticing better results so im guessing they love the new environment.

Looking back on this video and listening to it, I definitely don't need that much wind power lmao!

Day 73 Day 38 Flower Monday 6th Camera is doing no justice, little video, although to my own fault I did not have phone with me when feeding yesterday for decent pictures. I will update again on water tomorrow ✌️ So far stretch has stopped, the recovery from lollipopping and defoliating is immaculate, she is so strong, already seeing denser bud sites and focused energy towards tops. Day 74 - picture updates, looking great 😍💪💚

For LIQUIDS & Nutes ******GREEN BUZZ NUTRIENTS***** organic. Also i’m using their LIVING SOIL CULTURE in powder form! MARSHYDRO ⛺️ has large openings on the sides which is useful for mid section groom room work. 🤩 ☀️ MARSHYDRO FC 3000 LED 300W 💨MARSHYDRO 6” in-line EXTRACTOR with speed-variation knob, comes complete with ducting and carbon filter.

So I definitely fucked up this week and missed some crucial water times. I passively knew in the back of my mind to but I was just having busy and stressful days.

Final week

Bud sites are producing black/purple spot in leaves. Bud growth is producing as the days go by. Really excited with this being my first grow Nitrogen boost really helped with the deficiencies. Plants are starting to look really heathy... Prunes lower colas that weren't stretching past the canopy and receiving energy so more energy can be used towards the top buds

Day 32 ...This girl was too big for her 3gal container so I bought 40 Litre rubber totes to use! Drilled holes around the container and tied a ton of branches to the sides of the container with pipe cleaners. Plant was about 3 inches tall tied down and a 16 inch canopy. Follow as I veg this beast for another 30 days!

First week of flowering went well. See pic descriptions for details or hit us up if you want to know more!

Ha sido una de las mas productivas de las 8 que tenia en el pequeño armario. Voy a repetir esta cepa en mi siguiente cultivo ya que me ha encantado.

La segunda semana fue bien, a las plantas que estiraban más les causé Low Stress para mantener una altura igualada. De las 3 variedades que tengo, es la que antes ha mostrado las preflores. Un error vuelvo a repetir ha sido no tener medidor de EC-PH y otro ha sido el haberme excedido con los s riegos. Conecto pronto el CO2 a 630ppm par ver si así logro obtener un buen estiramiento esta semana. 📅 Día 15: 🔹 Cambio de lugar al haber quitado algún macho de otra variedad. 📅 Día 16: 🔹 Riego según la tabla de nutrientes. Conecto CO2 a 700ppm. 📅 Día 17 : 🔹 Low Stress para que dé más luz a las ramas bajas y tengan más espacio para desarrollar. No quito hojas ya que creo que les quitas los paneles que las alimenta. El riego de ayer las sentó muy bien y están bebiendo el agua rápido por fin. 📅 Día 18:🔹 Descanso, puntualizar que he activado el CO2 a 730ppm y decir que se han bebido todo el agua, mañana toca riego. 📅 Día 19: 🔹Riego según la tabla de nutrientes. La EC me dio 2000 y el pH 6.3

March 30th. Awsome growing Plaant, It feels like she doubled Size Flowerws appear more and more,,, iam very happy with those Girls They are drinking around 0,5l a day, Feeding every 3rd. Day Still stretching.... stay curious

Day 95 harvest of last 2 of 4 plants. 10 days after first two were harvested. BB1 and GG1 seem to have denser buds than BB2 and GG2. I think the yield will be greater also. First two plants dried for 6 days at 45-50 RH and 65-70F and put into quart Mason jars with humidity meters and pouches at 62 RH. Once second two have dried, will update dry weight for all 4 plants in Harvest week.

What a week. Set up my new light today, next week should be 🔥 Had a mishap during lst and broke one of my better branches. I bandaged her with frog tape without wrapping it. She responded well and is back at it. Think I’ll leave the tape and let her break through it. I’m going to start eyeing changes with my nutes now that I expect her to drink more under this light. I will likely start watering more than twice a week. Right now she gets 1L every 3-4 days. Lemme know what you think. I’m stoked fr.

Flush week

Wow 😯 I’m so amazed by this CBD plant of spiced orange 🍊 from BSB Genetics she’s on node 7 and I’m not sure if I wanna too her or let her just go?? What do y’all suggest?

Das Zelt ist voll und sieht super aus

Welcome back to my summer grow🙂 Everything is still in need for more, more light - more nutes - more airflow and last but not least, more ir/uv 😎 Also gaining alot of weight so that’s also great, might need to tie this one up asap😊 Hope to see you again next week 😎 ------------------------------------------------------------------------------------------------------- SpectrumX LED🔥 @medicgrow 880 Watt⚡️ 2x UV/IR LED panels 💥 2.7 μmol/J🔥 Full Spectrum V1,F1,VS,FS🌈 Display with PPFD & Live spectrum Light measurement: Apogee MQ-610 & Apogee DLI-600. Fertiliser: Organics Nutrients https://www.organicsnutrients.com/en/ Green Buzz Nutrients Discount Code: GD42025 Grants 25% with a minimum Order value at 75 Euro. https://greenbuzzliquids.com/en/shop/

Growing tall and beautiful