End of the first few days of the transition. They all got a significant defoliation to open up space in the canopy for branching and the plants responded incredibly well to it. Plants 1&2 started to show immediate deficiency signs after defoliation and I upped the nute ratios by about 50%/liter with the Berkana. Voracious feeder and growers these are for sure. That said, their growth rate is phenomenal and the side branching is especially impressive and responsive to training. Veg. Maturity on all of em for the last week so it’s a fast growing strain all around👌. Well throw down the scrog net in a couple of days after we hack out the lower portions of each plant and take clones for all of em but the runt (#8). The clones will be flowered fast in the aeroponic cloner and pollinated for F3 generation seed. Bud characteristics on this run will then determine which clones get tested first based on best characteristics of the lot. So far this run has been incredibly impressive but only the bud quality will tell the true tale. Here’s hoping for continued good progress. ***Grow notes: stretching at about 1/2” per day over the last 3 days of transition. Side branching reaching enough maturity to start taking cuts. Minor deficiency signs post-defol. So increasing nute ratios on a slightly accelerated basis continues to be the norm.

Loved these flowers

- Ich hab die erste Woche des Biobizz Allmix Düngeschemas gestartet und gebe wie immer erstmal vorsichtige 100%, da meine Erde an sich durch den Kompost recht potent sein sollte. - Außerdem hab ich mit meinem Calmag-Rechner mein Leitungswasser angepasst. Die Werte könnt ihr auf den Bildern sehen. - Den PH-Wert passe ich ebenfalls auf 6,5 an.# - Die Lampe bringt zur Zeit etwa einen PPFD-Wert von 350 - 360 µmol/s/m² bei 18 Stunden Beleuchtungszeit (DLI von 22 - 23 mol/d/m²). Sie wird aber nur eingesetzt bis es warm genug draußen bleibt. Tagsüber stehen sie jetzt schon draußen, sofern es nicht regnet oder stürmt. Die Guava macht sich hervorragend und sie ist sehr uniform mit den anderen Fastbuds Strains bis jetzt. Sieht gut aus finde ich 💪😁👍

Growing for FastBuds420 has truly been an honor their genetics are truly Best in the world in my book strange our name beautifully mimicking the smells and appearances of their names. Pineapple express is one . They nailed the name right on the head. This plant truly smells just like a pineapple and almost looks like a pineapple once the fade starts one of the biggest autos I’ve ever grown truly thankful for the opportunity to grow this plant FastBuds420. 4 Life 🌿💨💯

Repoted them into 5 litre pots from pot noodle cups , pulling top fan leaves still . The side branches are catching up slowly.

สัปดาห์นี้ยังไม่พบปัญหาใดๆแต่ก่อนหน้านั้นมีต้นที่ตายไปเกินจากแมลงกัดที่โคนต้นก่อนที่จะเปลี่ยนลงถุงปลูกก็มีบ้าง เพราะว่าข้าพเจ้าปลูกไฟแต่เอาไว้นอกบ้าน ใช้ลมธรรมชาติ อากาศสภาพบริเวณโดยรอบ ไม่ร้อนครับ แขวนไฟใบพัดไว้ดวงเดียวเพื่อให้ได้ชั่วโมงแสงเพิ่ม สภาพอากาศชื้นจัดแดดไม่มี เพราะอยู่ในหน้าฝนครับ เท่ากับว่าเราได้ความชื้นเต็มๆต้นก็ดูแลเหมือนจะโตดีเพราะความชื้นด้วย อันนี้ผมเดาเอาเองจากสภาพที่สังเกตุมาครับ 🤗🤣

•Todo marcha ok; hay dos Epic que están más desarrolladas.😊 •En esta semana trasplantaré a mecetas de 6L,y prepararé el abonado a usar. •Powder Feeding Bio🤙🏻 -------------------------------------------------------------- 🚀A dia de hoy: 👇 ▪️Editado: 15/10/23 -Trasplanté a macetas de 7L,tamaño final. -Mezclé en cada maceta 15g de feeding bio grow. -Y 2g por 2L,de Aditive powder Enchancer; está mezcla la aplicaré cada 12/14 dias aprox.PH: 6,🤙🏻

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Day 72: 600W LED, 18 hours on 6 hours off the same with ventilation. Ferilization is the same except BlackBerry Kush and LSD-25 they are being flushed. Water intake also remains the same 200ml per day. Humidity approx 35 percent. Day 74: BlackBerry Kush and LSD-25 have to be harvested this week Friday so no more water. Colorado Cookies and Northern Light next week so started flushing them. Glueberry OG needs a bit more time.

Defoliación leve🍀👨‍🌾🏻

Been slacking at updating this. Putting in a lot of OT at work and the diary has fallen to the wayside for a bit. Had to put the trellis netting up. The two Lemon Haze plants are getting chunky and falling over under their own weight. Looks like they only have a couple weeks left where as the two Grandaddy Purp on the left side look more like they have 3 to 4 more to go. Hard to tell as they don't seem to be swelling up as much as the other two. Getting so close I can SMELLLLL IT!!

End of week 11. Now I can say the scent permeates , if you lean in to take a sniff. It is a surgery-sweet, lemony smell( in the past, getting trichomes on my finger tips left that smell). Multi-pull buds are taking shape, but there's no clear winner, no cola I can call a main cola. If were to 'eye weigh it' each cola has about in estimated 2.5-3.5grams at the most. Plants are fed the same. 1 or 2 leaves show nitrogen deficiency.. will start to flush now(stopping nutrient feeding).

Also a fastbuds purple lemonade in a 11 litre pot (30grams)a a original cheese in a 2 litre pot(experimenting)which is not ready yet. Strawberry pie.....wow so impressed with the yield but also the looks are amazing and smells like strawberries... who’d of thought it lol. Recommend to everybody will defo grow again. Fastbuds are killing the auto game!👊🏻

Week 2 using Dutch pro grow A and B Dutch pro root solution Calmag , multi total

They’ve been over fed and underfed but they look and smell good. Buds are quite small but are rock hard, even the lower ones. Just running loads of rainwater through them to finish them off, has a ph of 5.8 and doesn’t even register on the ec stick so it’s perfect for flushing in hydro. Merry Christmas 💚💚

Al these strains are truly impressing me...couldn't be more happy...WEEDSEEDSEXPRESS is definitely my go to Bank..haven't been disappointed once with the genetics or service..

we pass 5. week, in this week i been spred my girls and make some defoliation. I had an accident when spreed but she doing just fine. grow nicely week by week. in next week i will flip them on flower mode. tent is ollmost full.

Wonderful harvest! Plants were very dense and frosty and smell was super strong. I made sure to wear gloves so my hands weren't sticky the rest of the day. Very happy with this strain, it seemed to produce quite a bit of dense frosty bud. Nice deep purple leaves at the end. Will update with a smoke report after a 6 day dry, 30 day cure in my Cannatrol.

Still struggling to get the medium pH up been adding Potassium bicarbonate last 2 waterings (in going pH 8.9) still getting out of range low 5 runoff numbers, left off Cal/Mag last time to make sure there is no acid in the mix. Just anxious to get this grow done, think I went to heavy on the AgroThrive early on thinking it was weaker than salts, it certainty is not, lesson learned the only other thing I thought might help is a help is adding microbes to widen the pH range but it might be too late for that at this point. 😕

Buds are starting to fatten up resin levels are high she’s getting sticky light defoliation where needed to help light exposure