Harvest Report – Blue Sunset Sherbert The weed turned out absolutely phenomenal! The colors were stunning – incredibly dark, almost black, with deep purple hues that really popped. It was harvested at Day 80, with about 20% amber trichomes – just the right timing for a balanced effect. Check out the pictures – they speak for themselves! Stay tuned for the smoke report! --- Erntebericht – Blue Sunset Sherbert Das Weed ist einfach phänomenal geworden! Die Farben waren atemberaubend – richtig dunkel, fast schwarz, mit tiefvioletten Tönen, die richtig hervorgekommen sind. Geerntet wurde bei Tag 80, mit etwa 20% bernsteinfarbenen Trichomen – genau der richtige Zeitpunkt für einen ausgewogenen Effekt. Schaut euch die Fotos an – sie sprechen für sich! Bleibt dran für den Smoke Report!

Everything is coming along quite nicely. They have slowed down in growth now it is time to fill out. Still feeding fish 3x daily. They have developed a pretty strong smell

This week was very hot. They stressed a lot some heat stress and some fan stress that came up to uptake look down. The weedding cake was that must sofer. Shr was still recovering from that led issue...and she gets direct sun light in the hottest hour. Black cream in the other hand sofer a lot with fan stress bleaching the leafs in direct contact. Couskush its a beautifull lady and growing nice. Bomberry is late but showing some promissing buds. Chears BrotherHood

Mr. Pipi defoliated a bit after the topdress last week. just some old leafs here and there, not much. And Frost is building up, terps are coming. Mr.Pipi is happy :) AND the colors BOI oh BOI. thats what Mr.Pipi dreamt of. Day 46: Heights : Frank 47cm , Pedro 44cm and Lance 34cm.

09/16: Fed 30/70 blend of Veg to Bloom to all the photos. Noticed PM on True OG. Didn't notice yet but my OBW (and some other autos) also had a bad PM infection. Light defoliation and trimming 09/21: More PM found. Again light defoliation and trimming. Aphids also found on some of the autos.

Starting this little diary already a week in. This blackberry started flowering at day 16, I dropped the pot and I guess the shock of that started the change. Stick around to see how she turned out.

This week the plant was bended, and accidentally i broke a branch ... But she recovered well, she selected the other branches to grow just like in 2 h nothing could be done, but as you can see she is concentrating the growth in the other colas, so the structure is bended against the pot and with 3 main branches at the moment... Another little problem appeared when, 2 days after using Green Explosion in a half dose, i raised the Top Veg to 2.5 ml/l but she reacted bad and some overfertilization spots are apperaing in the tips of the leaves, im uploading it when the next week is complete... But dont worry is not too much overdose, just a little, (Its like she said thats the limit at the moment ) so after this, next 2 irrigations are being done with plain water to flush the soil... Im not using Micro Vita, but i gotta get that magic microbes to avoid this messy things, they muffle all this changes in PH !!! So next week, transplanting to definitive text pot, bacterian life and until the end :) But everything is fine this plant is soo strong and nothing cant stop her at the moment :D Take care GD !!

The biosys is once a week when I cut off the res and can see the tray starting to empty out at the bottom. I then hand water some Ecothrive Biosys to aid the Ecothrive life-cycle in that second to top layer as well as the grow dots in general (it’s just basically a cheaper UK version of recharge, in that it has a lot of the same ingredients) only I figure they are more likely to be suited to my weather conditions if they are grown here. It’s also all REALLY GREAT stuff to add onto my cactus collection - works great! I just going to carry on - this has been the easiest grow iv ever done so far. All you need to do is PH the water and fill the res - all the microbe shit is optional - although IME boosts plant health and therefore yields. My last grow I got 1.8g/w and half of my grow space was taken up with the actual res! I didn’t think to put it outside my 3x3 (ish) tent! Still tho, that’s respectable numbers. Think it worked out to about 3z in total

Day 21. Fed just over 1.5L only yesterday and they appear to be really thirsty girls with the pot already feeling light however with the slight curling of Leafs maybe suggests slight overwatering. Im also thinking a very slight mg deficiency so will add a little cal-mag on the next feed. Gonna wait at least another 24 hours then Introduce bloom and top max. Would like these to stretch a lot over the next week. Already starting to smell. With this tent being in my garage and the outside temps dropping from 10 degrees now down to 0 the temp inside the tent has gone from 27 to 22. Turned extraction fan down to help hold the heat but with the night 600w HPS on 24-0 as well as plenty of inside airflow, absolute no issues. Day 23 Just shy of 2L per plant. Responding well. Very early signs of flowering, still stretching about 1” daily really wanna see these shoot up over the next 5 days Day 25 currently 12-14” height. Plants are thirsty so another 1.7L fed today.

Thats it, last fan leaves are yellow and those girls are ready, they still pack up, loads of new pistils visible, despite all going brown from low temps during greenhouse period. I start to see ambers a lot, they are not so leafy and i lost 2 main colas to bud rot, better safe than sorrow !!! Theirs calyxes are well matured all stems browny pinky, they made a journey in full in my eyes and as always i still hate yellow leaves, don't want to slaughter bud while trimming. Think two more days, then 48 hours of darkness and late sunday eve - they are chopped and wet trimmed. Thursday. Added couple closeups, but they are far better then in photos, bulking up each day, packing trichs like crazy. This time buds don't look so lanky, from airy leafy crap, they become solid nugs... Its my third different grow of this strain. First 4 plants and Biggest clone looked really similar, this ones - more Real GG4 structure, love it !!! All got lite pinky , violet colours . All have fruity smell, just not last ones ;)) they are wild strawberries + . Amazing flavour of early buds tells me final harvest will be fire !!! Have stupid feeling, taking them down too early, but my yellow leaves OCD whispers me " Cut, cut, cut ..." ;))) Chop Chop Day. Wet trim chosen, they are sparkling !!! Fat, but still leafy ... Found 3 budrot spots, two would eat big colas, but low humidity forced those leaves just die off without infecting others, so it was perfect timing. Happy Growing !

Oh yes, my friends .... Peaky is back stronger than before to compete with the best! Take a look at my updates The best crops and best regards

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Nice plants 2 totally different phenotypes, as one was already harvested, and the other isn't even half way of flowering after 10 weeks. Nice trichome production, great fresh and fruity smell, this plant is a pleasure to smoke in the end of the day. Dried her upside down hanging in a tent I have for the purpose, at 25c, 30%RH - Only removed fan leaves, no manicuring, been doing that as I smoke, noticed the bud lasts longer and with a better smell and you end up saving loads of time. Really happy with this smoke, smells like proper weed. Maybe was the best time I dried and cure(ing). Only problem is that I'm smoking more as it's so tasty ahaha. Good stuff, thumbs up! PS - I'll start looking for the "original" Zkittlez to try out and check the differences between them. Update: 12 April 2021 - Chopped down Plant #2 So, finally Saturday I chopped her down. She looked now ready and delicious, although I only notice yesterday how many seeds she has allover the place. She hermied in front of me without noticing, not good at all and need to start looking much better and in detail into the plants. Feel like an idiot now. Anyways, it was a full week! Emptied the grinder collector and pressed it down a little, makes a very nice smoke, light but pleasant, the kind of stuff it keep you going all day without the dumb effect. From all trimming made the bubble hash. Got a little too green as the 220micron bag's stitches slightly opened letting some contaminants in the final mix, hence the green color. This was a hit. in opposition to the grinder stuff, this sent me straight to the moon, really heavy stone effect, even thinking was hard. Reminded me those afghan/ moroccan balls we used to get back in the early 2000's. The weed itself it's still drying so the smoke report will be updated in there. 715g of fresh cut plant is good, let's see the potency and the seeds. Might turn it all into bubble hash, if I see it'll be an hassle to remove seeds. On an earlier bud I've cut and dried, smoked it and you can see pure white ashes in the bong. Ain't that a beauty?

Well well exciting times ahead my favourite time multiple buds sites appearing all over I'm so impressed with the new Mars hydro ts1000w the growth is amazing. I have to say this strain is the most smelliest I've grown very pungent cannot wait for the coming weeks to see these buds stacking some weight

They are all showing preflower and chugging along nicely.

Hi bros hope you are doing great ! New diary new adventure and hopefully new colors and flavors before i get to the details i want to give a special thanks to everybody and i mean each one of you for your grows i gained a lot a knowledge being here so thanks to @growdiaries for this great platform and thank you guys ... for this grow i have 4 tents and difference set up and methods for each one of them 1 tent is 80×80×160 with 400 watts hps with 2 mexican airlines in 15L pots (LST) 1 tent is 80X80X160 with 250watts hps with 2 lsd-25 in 15L propots (LST) 1 tent is 80X80X180 with 400 watts hps with 1 chocolate mint OG feminised in 20L propot (Scrog) 1 tent is 120x120x200 with 400 watts hps with 3 sixshooter and 1 lsd-25 in 15L propots (LST) Im only showing you guys LSD25 with the LST And the Chocolate ming OG under SCROG The other 2 grow tents being LST'd aswell ill show u only the harvest but i will do the same low stress so you will be able to pick and choose from the method you like the most. Advanced nutrients and plagron light mix for six shooter lsd-25 and chocolate mint OG VS biobizz for mexican airlines ( i want to see what is best for me! ) Switching saving lsd's for another grow got a free cheese CBD from dinafem and free so imma try that first

Started the week with a big mid flower feed with NFTG. Also shaved her legs up a bit and defoliated some leaves blocking some light. She is growing tall for a indica and her leaves are more similar to a sativa imo but regardless she is growing very well! Day 57- NFTG mid flower feed/ Mammoth P/Recharge @ 6.5 Ph Day58-59-No water Day 60- Recharge/Cal-Mag/Mammoth P/ Bloom Khaos Feed @6.5ph Day 61- No water

starting to shape up into something

Just an absolute beast. Thank God it is the last week of veg, theres nowhere left to go but up. About 3 feet now and should finish around 6 feet. Will be quite a feat for a small space, its sharing a 2x4 with another mother slightly shorter. May need to tie down in flower if it overtakes the other

It’s hard sometimes, resisting the urge to feed her with her waterings. But I’m by now it’s gotten much easier. I wasn’t able to do the entire grow with plain water, and maybe that was better. I can’t help but wonder if the small buds is from a lack of food or if it’s just genetics. I can’t necessarily say lack of food if I study all her leaf tips. After noticing very minimal, yet noticeable, signs of tip burn, I feel confident saying she is getting lots of food. Wether or not it was the right ratio of food is another question. Maybe there wasn’t enough phosphates? Just because there isn’t a deficiency, doesn’t mean the plant couldn’t have been able to utilized more. I might come to find these tiny super dense buds.