2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Whats is wrong with my plants? I don't know If you know please advise

4 out of 4 and all of them are very healthy and strong babys so i am waiting for a good yield lets see whats fast veraion of this strain can offer to me. Many thanx for the seeds to the one of the beat companies in thia business. So happy 420.

Day 23 of flower! If im correct. I'm here a little sick but trying. The girls are doing very well 1 of them is very very bushy while the others are magnificent 👌 the spacing and the way the girls grew their branches outwards is beautiful. I did not do my best this grow because I simply don't have the equipment I need and alot has been happening lately. I'm glad I'm going to have some top notch smoke in a couple of weeks because they smell SOO SWEET.... I never smelled something like that.. it hits your nose and goes all the way down.

Hey everyone :-). There is not much to tell this week :) A few came to the flowering tent, 2 are still in the vegi phase for 2 days and then come last to the flowering tent :-) The Blue Cheese and the Kosher Tangie Kush smell very good and how they should 😍👍 It will be difficult to choose 2 mums :-). I wish everyone a nice week 👌 Let it grow

Super easy frosty smell super strong and sticky

Gorilla Jealousy F1 is doing good. She had a solution change done about 5 to 6 days ago. She is about half way through flowering. Her colas are bulking currently. They do have a nice layer of frost on them already. Everything is looking like good at the moment. Thank you Spider Farmer, and Seedsman. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 https://www.seedsman.com/?a_aid=Mrsour420. This is my affiliate link to seedsman. Thank you Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Week 19 and it happens to be a rainy week with on and off cloud coverage which was actually beneficial to my plant but the humidity has been up due to the fact I also use a swamp cooler and if it's gonna be this humid for another couple days then I'm worried about mold developing on the buds so i think I'll break out the AC to get rid of some of that moisture in the air and maybe alternate between the two. The AC hasn't been used once the whole grow cycle so far but living in AZ we get random storms from the monsoon season not to mention tons of dust, high winds, flash flooding which could be used to flush my plant because it needs it now if rain builds up around and gets under the green house, j/k lol. I've been thinking about buying lady bugs to get rid of all the white flies, any experience anybody, would that work? After writing the above, I could hear the wind picking up outside and remembered that a storm was coming so I ran outside to prepare and it punched it's way through Phoenix with extremely high velocity hurricane type wind, gusting up to whatever it takes to bring large trees down or make a wooden shed door fly off with a wall of dust to the sky and then finally finishing with heavy rain. I was outside the entire storm from beginning to end, well at least until the wind died down and it was just light showers by then. Even with having my greenhouse tied down I had to hold onto it for dear life or it would have taken off like in the wizard of OZ. Honest to god I was almost pulled off the ground or several times more onto my greenhouse when the tsunami type wind would hit, pounding me and the greenhouse from behind having to pull down while leaning back with all my weight and strength.The whole time I'm screaming for my brother who was inside to come out and hold the greenhouse so I could tie it down better because it was just coming apart trying trying to fly away or be ripped apart as storm hit us I was holding on for Amy's Green Drop's dear life. It was pretty insane and wish I had video of me doing it but just have a video a friend sent of his backyard getting hit so you can imagine that wind hitting my greenhouse. The after math was bad 😭 the wall of the greenhouse was getting hit so hard by the wind making it press inward towards plant it snapped off in the middle one of the lower branches making the two buds on the end half hanging there from a 90 degree angle from branch so I knew there was no way to support it back in place enough to heal itself so I clipped it off and started drying it. One other next to it got bent up pretty bad so I'm trying to LST it back to place. The storm also killed my swamp cooler somehow, I guess it's because it has a lot of electronic components on top that was soaked from the wind the rain came from the side, I know, you'd think a swamp cooler could get wet. Now I'm stuck using only my A/C and a humidifier to control moisture and temp and I'm worried it's gonna be pretty hard only using AC in such a small greenhouse as it takes time to find the right ratio of intake and outtake air but can only open greenhouse to mix in so much hot dry air, so starting it was getting down into the high 60's, and I have to figure something out because I can't let it get that cold in there for the last few weeks. I'm almost ready to call it quits If I can't find an equilibrium with AC and humidifier, and if there are anymore storms on the way. Next time I'm getting a bigger sturdier greenhouse that goes into the ground and has hard clear walls and roof, other than the recent storm and excessive sunlight finding the right shading it's been very beneficial doing the indoor/outdoor combo grow and would recommend it as you can live in any weathered extreme hot/cold climate and still grow outside,

another week on full dose. 3-4 weeks remaining.

Week 7 Mars Hydro TS1000 has arrived. plants starting up te flowering stage. did some light defoliation.

Day 69 (March 6th) Just gave everyone their last watering. I’m going to harvest and wash everything tomorrow night on day 70. I‘m not going to do that whole 48 hours of darkness thing because I didn’t see a difference last time I did it. In fact, it made my plant under watered before drying which caused it to dry too fast. Slurricane and cheese will be washed with h2o2 and the rest will just be rinsed off in warm RO water and hung up. Before and after washing I’m going to go over each branch with a flashlight and make sure there is no dog hair or debris in or on the buds. I will be drying in my spare bathroom (that no one uses!) from hangers. Aiming to keep it around 60-62F and 50-60 RH. Hoping for that 10-12 day dry. I wanted to do a full plant hang but the tent got pretty dirty and I would feel more comfortable just cutting and rinsing everything off branch by branch. I’m going to try my best to keep the full plant intact. Got my humidifier hooked up and running RO water because with tap water it deposits calcium and lime all over the buds and walls. For air flow I’m just indirectly running a small usb fan. I’m not worried about air flow in the bathroom honestly. It stays nice and breezy down there anyway. Got the food grade peroxide and RO water to wash up the slurricane and cheese. Everything except for the cheese and garlic#1 is looking more than done. Trichomes all look pretty well done. Some of them have even burst open. Smells are all super ripe and mature too. Can’t wait to smell each individually in jars. Next week I will recap what went wrong and what I will do differently next time to keep it from happening again. (Day 70F) Just got everything chopped, washed and hanging. The vast majority of the PM was washed out but I can still see very small amounts. When they dry out I can shake the branches a bit to get the rest off. At very least it’s sterile now. There is no question I lost some potency while washing but the amount is negligible for a much cleaner product. I could see lots of dirt and other debris float to the surface of the water after being washed. And no those are not trichomes, those sink to the bottom. I could put that nasty water through a bubble bag and wouldn’t get all that much. Although it probably does remove a lot of the actual trichomes heads. I’ll have to check with the microscope. Everything is rigged up for a nice slow dry. Temp is sitting at 64 and RH is at a nice 60%.

This week has flew in , can’t believe I’m on week 5 already :) . The girls are growing well , allow they are 2 totally different looking plants the smell the same and seem to be maturing at the same rate :) I can’t wait to see all the little bud sites start to fatten and fill out over the last few weeks

Slurricane #4 Yield: 34.5g Smell: 8.5/10 - gassy, deep piney, fuel, earthy, Bag appeal: 8.510 - slightly leafy/stemy Crystal coverage: 7.510 - impressed Ash: 6/10 - flaky, mostly white/grey Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 6/10 - taste is decent High: 6/10 - indica dom Comment: looks amazing, smells amazing, tastes nice, burns like shit. 46.5/70 = 66% 👎🗑️

This week the plants are developing very nice new shoots with a good stretch as well a good amount of bud sites.

Day 51, 9th of November 2021: Hi there! Here we go the second week of flowering. This is the time really not much to say apart from the strech they are going through. Especially Spilff's Strawberry 🍓 she is crazily doing it. Not much changed maximum a bit more water intake for them. I could write ✍️ review each of them but nothing significant happening it is the transition stage anyways. So rather just post the current settings and facts basically it is just the same like last week when it started. ViparSpectra XS2000 is on full power which is 240W so 100% capacity. Ventilation is different as well under the 12 hours period when the lamp is on it switches 1 hours on and after one hour off mostly to keep the temperature and of course because of fresh air flow. When the lamp is off it switches on in every 2 hours for 15 min at the same time with the extraction fan. The extraction fan is on 12 hours when the lamp is, and then it switches on in every 2 hours for 30 min. That's all a bit more water for them from today also. Humidity quite the same approx 50%, temperature as well approx 26-27 Celsius. Fertilization remains the same since last week, the BioBizz family raised up to be 2ml/L each so it is like 2ml/L of BioGrow, 2ml/L of BioBloom, 2ml/L of TopMax... see the ratio above. The schedule of the fertilization has not changed happnes 2x a week except epsom salt just 1x a week.

Week 12/2 - 18/2

Hey there Just chilling now and letting them do their thing. Been pretty chill so far, dechlorinated water and top dressing. Going to give some more microbe life as well and maybe make an SST sometime this week.

Week 2 of flower for these girls. I decided to use a lesser dose of nutes because I noticed after a few days after my feed last week that there may have been some leaf burn. -I changed up the nutrients a little and also finished putting up my inline fan - pulling fresh air from outside in for CO2. I simply used ducts for it. Now that cooler air is coming in from outside I can slowly turn up my lights to 900 watts, I’ll see how they do in about a day or 2 more and then the lights will switch to 1000watts. -Did some more defoliation. The leaves grow so fast!! Took 2 days to do all my plants lol I’m trying to do it every week or other week -The plants are drinking a lot more than they used to, I’m now feeding water every second day and nutes every second day! They’re growing so fast before my eyes It’s such a cool feeling seeing all my hard work coming along, with the first buds popping up and looking like real weed now. Feeling motivated! 😇