Smell extra strong when snipping some leaves down ! 6/10 Dry done Cure start

Coming in a day early this week because tomorrow is Thanksgiving. Plants are growing nicely, pistils and colors and frost, all good stuff happening. None of the colas are fat and solid like a pinecone yet, but we're on track for a nice harvest. I'm thinking 4 more weeks.

Week 4 already!!! The plant is doing great this week, the weather continues to be great so hopefully it stays like this until the end of flowering! September 5: water September 6: nothing September 7: nothing September 8: water September 9: nothing September 10: some more spray with spider mite (only bigger leaves, of course not on the buds) September 11: nothing

Final week!

She's doing great. Beautiful Leafs and a good height for her age. Let's Go veg!🤙🏽

hace tiempo tenia ganas de cultivar esta cepa, de buen crecimiento y aroma dulce. muy buena produccion de cogollos y resina, resistio muy bien algunos problemas al inicio del cultivo en la poda apical y posterior LST, buena cepa para LST muy frondosa

Day 113. Third wave of heavy flush, girls get from 30 to 50 liters in time-lapse of one hour. Planing to chop them after 10 days, two more heavy flushes ... Control Garden is a beauty ... They are a bit behind, but hopefully next week i will start to flush them aswell. Incredible Bulk standing in front of fan is most matured aka damaged ;))) Not many seeds i see, so i calmed down a bit, still free cheese is only in trap, fact ... Day 115. Girls fade nicely, today got 4th wave of flush, best case scenario in 8 days chop, worst - in 4. Busy work week, so it will be hard to find time, wet triming takes to much from me, i do it too carefully ... Happy Growing !

All three plants are hermaphrodites, however, plants 1 and 2 are showing minimal signs of male with seeds developing only on lower nodes, while plant 3 looks mostly male with a few pistils only on the very top nodes. I've decided to pluck the seeds from plants 1 & 2 as they develop, and to toss plant 3 to make room for the other 2. It's not ideal and I plan to buy feminized seeds or clones for my next grow, but the bud I got the seeds from was actually really good so I'm going to try to take 1 and 2 to harvest.

Welcome to my Ðivine ØĠ Ķush diary. In this diary: Seeds: sponsored by Ðivine Șeeds Media: Promix HP Nutrients: Advanced Nutrients, Diablo Nutrients, Gaia Green Power Bloom. Light and Weather: Şun☀️and Mother Earth.🌎 ___________________________ Feeding: Wednesday, Thursday rain🌧️ Fri 23Aug: 4L nutrients pH'd 6.5 Sat 24Aug: 6L water not pH'd Sun 25Aug: 4L water not pH'd ___________________________ *please note that most watering are from top now since topping with Gaia Green Power Bloom*...... and since i smashed the saucers with the weed wacker🤦🏻‍♂️ ______________________________ Ķush Ķush is on autopilot. After 4 days straight of rain and colder days, nice weather came back for the weekend! Yay!!☀️ _______________________________ Top dressing her with Gaia Green Power Bloom was and is still a smart move as the sugar leaves got a nice green colour. ______________________________ Thanks for stopping by, likes and comments are appreciated!👊🏻😎 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

Great first harvest. I took her out of the tent a little early but I'm still happy with the results. The last two days the plant was under total darkness. I will update with dry weight and more details of the nug in the next weeks. Just hung up the stalks to dry. Thanks to the people who helped with my fungus nat issue as well. Ended up with 107 grams of dry nugs, couldn't be happier. Can't wait for my new grows!😎00

Flower: Switched the lights to 12/12 yesterday. I plan on moving them into my new 4x4 tent as soon as my AI controller arrives. Unfortunately, the AC tents have 22mm thick poles and my PVC scrog net won't work until my Etsy parts come. But these girls hardly needed any training. I pinched the tops maybe 4 times during veg. (right below top node and pop the stem like a pimple but do not break any tissue on the outside) and also used the 90degree elbows to bend then tops twice. Other than that great plant structure from Twenty20 genetics. Top Dress: I made a pretty strong super soil and didn't top dress all veg in my 3G pots. I did make 2 bat guano teas. I did just top dress with some Build a soil craft blend and made a bloom tea. Also sprinkled on some Kashi and added the coco soil covering and already seeing that white fuzz growth. Veg issues: The close right plant got transplant stressed causing it to bush up and the internodal spacing to be very short. when transplanting it lost structure and fell over so I had to compact the dirt to get it to stand back up. But overall, it bounced back. The back left has been dealing with either light issues, pH issues, or dry pockets in the soil. I think its light/heat. But after learning peatmoss can be hydrophobic when over dried out and cause dry spot and fox farms uses peat moss this could be a possibility and why I'll be pre wetting my soil when I mix next run. I moved my heater around and fans and nothing seemed to work other than lowing the lights to 2/10. Could be a K issue as well and I just fed them a bunch so maybe that fixes it. Light issue: I think being in a 2.7x2.7x5ft tent caused the EVO4 light PAR to bounce back into the plants and be way more efficient than what I expected causing me to constantly have light issues my last few runs. I finally folded and set them 2/10 for the remainder of veg and saw some promising signs. I think in a bigger tent it will allow for the light to travel to the sides and be less potent in the center allowing me to raise it up to 6/10 with better coverage, when using the TSW2000 in my 2.7x2.7 it seemed more spread out and less intense so ill be going back to that for next veg.

Half doses of Indo Nutrients, Full doses of Sticky Bandit

Feb 11th Week 8 is off to a good start. - lite-cycle; 11.5 hrs on, 12.5 hrs off - reducing rapidstart / CaliMag - increasing molasses - Reducing Volume of water > 14L next feed. Only 4 feeds till flush if all goes well. Feb 13th - USB scope shows that there is still a lot of development to come. Trichomes still showing clear in many areas. - 3 areas were checked & it’s 50/50 with clear / cloudy trichomes. Week 8 of Flower from a proposed 11-13 -no food tonight, soil still damp 15th - Lite cycle down to 11 hrs on, 13 off Feb 16th - reduced UVB time by an hour. Using 5 in total ( 2x 2.5 hrs ) - down to 3 feeds; before flush. Trichomes will be checked 1st - raised light a bit ~ 19” from closest Bud - planning Flush -targeting 40% RH 18th - finishing week 8 of Flower with a proactive move on shortening the lite-cycle again; 10 On - 14 Off. Goal is to promote Resin production, Bud-filling and hopefully bring the show to a tidy finish, on-time. - Took a few pictures last nite, posting later today - Calyxes really seem to be swelling and filling in

Im goong to start a to flush one of my plants this week for 10 days the other one is a few weeks away yet

Hey everyone! So this week has been fairly straight forward and noticed some deficiency of calcium / magnesium that i'm in the midst of either a) do I treat it with 4 weeks left of flowering or b) do I make sure to add in kelp meal into my next grow for autonomous feeding, allow the plant to uptake on it's own. Going to go with option b since I noticed on my cream caramel grow, this deficiency was starting to show in early veg and thought nothing of it. With this being shown so late in the flowering, i'm thinking she is sucking up all the leftover nutrients before "dying off" and budding. The tent is holy stinky, i'm glad the carbon filter is doing it's job but I tell ya, watering is such a delight :) Buds are getting larger by the day..sweet skunky smell :) I count on one stock over 30 flower's that seem to be about toonie size, maybe larger, but get fatter by the day. This strain might be one to keep in the books to grow again :) Bye for now guys!

Its been very hot these past days in Ontario and the outdoor grow loves it! Plants have been growing nice and tall, growing some fatty leaves, I wanna see how they grow without any human interference so I will leave them without training for now. The soil is still providing nutes for the looks of it, I have started spraying some natural homemade pesticide you can find the recipes on my Instagram @girlgoneweed (under the highlights) I have been spraying 2x a week to keep rodents, aphids, mites, and mildew away. *The video is a dark angel as well but this one I have it on my roof garden.* Thanks for following along, see y'all next xoxo

Time challenged again so comments a a whole lot of shit talk are in the video.

These girls are getting really close. Definitely stacked amazing but from the looks, Definitely sativa dominate. They're getting a strong pungent OG smell and a couple have a super sweet smell. Excited for the next week to see how they progress.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.