All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Space is limited and I'm struggling with the time to keep up with these plants since things are back to normal on my parts of town. Just gonna keep about eye on em at keep defoliating

it's amazing, this growth looks much better than my first attempt, my decision was to put a smaller pot and change the light - the girl really liked it, FastBuds goriilla cookies with a small space manage perfectly:).

c:

We’ve hit week five of flowering, and things are getting really exciting! 🌸 The plant is now covered in trichomes, and many of them are already turning a milky color—such a great sign that things are progressing perfectly. The buds are starting to look really frosty. ❄️✨ I’ve been following the BioBizz week 6 feeding schedule, as it’s almost time to flush the plant—just 2-3 more weeks to go! 💧 I’m getting more excited each day as we move closer to harvest. Can’t wait to see how everything finishes up! 😁🌱

Cool plant. Strong stems. Big trichomes. I reset the clone, and it rooted in 5 days... the speed of this plant is really unbelievable!

These videos are from yesterday and we’re on day 5 of flower. Been MIA trying get this girls back to being healthy. I think they’re pretty healthy these days for sure. I did a bud leaf strip yesterday and some super cropping as well. This strain takes 8 weeks to flower completely. Looking forward to seeing how they turn out! ✌️🏾

Todos los días actualización nueva, pendientes !!

Day one of flower will start tonight 11/15/22 not really any growth for the small pot the beach ball was y’all responding well I guess it’s not as restricted as far as soil wise

Buds are starting to get nice and juicy aswell as big. Im now looking for a few of those amber trichomes before i start the flush. The nuggs are super sticky and get stinkier by the day. Peace Charlie

Comienzo del lst en dos de las plantas que están más sanas

Continued steady growth. Slight aroma appeared

Blue Dream'Matic is going well the bud sites are starting to get fatter and fatter. I kind of expected this one to stretch more and grow larger, but she still has several weeks to go and I think the buds are going to be huge! 💪 Added Fox Farms Organic Big Bloom to the feeding this week with Recharge. Looking forward to the results.

12/12 + 109 jours Vu qu’il y a 16 plantes mais que sur growdiaries on ne peut mettre que 8 variétés j'ai divisé en 2 diaries pour le bas de la tente 1️⃣ 🏠 90x60x90 ☀️ FC-E 4800 => puissance a 75% 🍁 Black Bomb / Philosopher Seed Amnesia Lemon / PEV Seeds Amnesia Lemon /PEV Seeds Blueberry / PEV Seeds Blueberry / 00 Seeds Wappa / Paradise Seed = 11g Dark Phoenix / Green House Seed Quick Sherbet / Exotic Seeds Mango Cream / Exotic Seeds Banana Frosting / Sensi Seed = 11g Hindu Kush / Sensi Seed Mix 1 / Sweet Seed Mix 2 / Sweet Seed = 15g Mix 3 / Sweet Seed Mix 4 / Sweet Seed 📎 https://growdiaries.com/diaries/122084-grow-journal-by-soosan 📎https://growdiaries.com/diaries/124052-grow-journal-by-soosan 2️⃣ 🏠 30x60x50 ☀️TS1000 => 100w = 54g 🍁 Quick Sherbet - Exotic Seed = 12g Quick Sherbet - Exotic Seed = 12g Quick Sherbet - Exotic Seed = 17,5g Quick Sherbet - Exotic Seed = 12,5g 📎 https://growdiaries.com/diaries/122080-grow-journal-by-soosan

Day 43. Girl is running nice. Now when 90% of side shoots are secured, just readjusting ties and leaf tucking left. Doubt ill make it in time for event, but somehow i feel that harvest will be way bigger reward ;)) she already smell strong and nice ;))) TS1000 is on max output. I rotate two smallest plants under her daily and see no issues with this light at the moment. Bud development will finalise my feeling about her ;) Happy Growing !!!

Flower week 3 and Lady Runtz still happy af. From now on it'll be fun to watch 💚. I gotta admit that Plagron is so much better than Biobizz. I'll definitely stay with Plagron 💪🏼

That's how I know the crop was harvested too early:( ..... but only because someone started visiting my place of cultivation and took my two girls, and left two, so that he wouldn't come back a second time and take them, I decided to cut her down earlier:( that's it such news awaited me when I returned from vacation, but overall the girl looked very beautiful, although she got a lot of stress when the snails ate her, but she endured it and recovered very well:) I will dry the bigger flowers and make bubble hash from the rest, good luck to everyone be careful in nature :).