Ended up with 4 mooning panties seedlings!!! Way excited to have these going these genetics will get any weed nerd Gitty. Still just doing declorinated water ph to 5.6-5.7. Just keep swimming Just keep swimming 🐠 Stay posted going to get turned up week 2 is always more growth!! Much love as

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Esta semana tuve dudas con el riego, como en casi toda la etapa de la planta, pero al fin, la veo super bien. Le doy 1 Litro de agua cada 3 dias, y parece que le encanta! Las ramas principales miden entre 70-80 cm, la mas grande llego a los 90 cm ! Ya no hay lugar para que siga creciendo mas alto, ya que no puedo subir mas la luz. Excepto ese asunto, todo va de 10 puntos! Ya estoy saboreando mis primeras cookies. Paz!

Start of week 4 flower, everything going awesome, she is absolutely piling on size already I am surprised to see, expect flower to finish in good time as a result, but we will see how it pans out :) So many sick tops, fattening up and starting to form crystal too, she smells superb

Lei sta ancora bene purtroppo i rami che avevo fasciato non hanno migliorato per niente la guarigione adesso proverò a sperare che non si creino condizioni per il marciume.. comunque del resto la pianta ha iniziato bene la fioritura e come vedete ha già dei bei calici da gonfiare😃 Per essere la prima coltivazione di Femminizzate outdoor direi discreto.

The biosys is once a week when I cut off the res and can see the tray starting to empty out at the bottom. I then hand water some Ecothrive Biosys to aid the Ecothrive life-cycle in that second to top layer as well as the grow dots in general (it’s just basically a cheaper UK version of recharge, in that it has a lot of the same ingredients) only I figure they are more likely to be suited to my weather conditions if they are grown here. It’s also all REALLY GREAT stuff to add onto my cactus collection - works great! I just going to carry on - this has been the easiest grow iv ever done so far. All you need to do is PH the water and fill the res - all the microbe shit is optional - although IME boosts plant health and therefore yields. My last grow I got 1.8g/w and half of my grow space was taken up with the actual res! I didn’t think to put it outside my 3x3 (ish) tent! Still tho, that’s respectable numbers. Think it worked out to about 3z in total

J’ai eu 1 amnesia lemon morte sans raison a la fin des deux semaine, du coup ca m’a obligé a faire germé une nouvelle amnesia pour la mettre directement en floraison

Looking great so far. Removing more fan leaves every other day or whenever i find the time to check the garden.

Good start so far for the girls . Proper good roots for only a few days . Already 2nd set of leafs have come out . E.C is still 0.6 although I will bump that up later in the week .

Tag 83: Mondhöchststand, Blütetag am Ende der Erntephase und Beginn der Wachstumsphase. Idealer Erntetag.

Welcome to my first grow! SET UP -------------------------------- Grow tent 2 x 3 x 6 hidden on a closet LED Panel VIPARSPECTRA 450w (200w real) Air filtration VivoSun 4 in Air circulation: 120mm computer fan GROWING ---------------------------- Medium (red solo cups): 75% Pro Mix Organic Seedling Starter Mix + 25% Perlite Medium: 80% Pro Mix Organic Vegetable & Herbs Moisture + 20% Perlite Smart Pots VivoSun

Hi everyone 👊🏻 thanks for checking in 😏, so we have started flowering this week on the bigger tent using the 2 Lumii 720w LEDs, we might or might not run into some hight issues well see… And we have the other 1.2 tent set up for the clones there under 1 Lumii 720w led delivered until I can another one sorted few weeks 🤙🏻 get them under that for a few weeks then get them flowering as well. Keep eyes open for progress 👌🏻

Lemony gassy terps overall I love his strain. First ever grow organic coast of Maine stonington blend and teas in a 3 gallon

A busca pela colheita perfeita

Fruit Automatic - Dinafem Tercena semana de cultivo. Es una planta de cannabis vigorosa, frondosa y con cortos espacios internodales. Una planta que se adapta bien al cambio de dosis de nutrientes. Además, la aportación de quelato de Hierro realizada semanas anteriores para la estimulación de la clorofila esta dando grandes resultados. Siendo una cepa con una dominancia sativa, debe estar presente su fenotipo de Lowryder ya que presenta un corto espacio internodal. Espero que según se vaya desarrollando muestre fenotipos de la Grapefruit !

Floracion día 1 EC 0.9 Ph 6.0

A lot of rain and wind, continuously. Temps have dropped as well. I’m reading another week or two to chop this plants. Sativa dominant hybrids did the best, the Fastberry and the banana purple punch did not really develop as the other two. In specifics the cbd crack developed sizable buds, and the blue dream as well, the flowers are not as developed tight and dense as in the indoor grow. A bit of fox-tailing nothing too expressive. Minimal work, as per the setup chosen, an no extra nutes this week.

Afgoo is an indica and candyland is as a sativa heard that talking to plant will increase nodes and increase overall yield first experience 3 days after topping these pictures are taken