2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Start of week 12: 4/29/25 Fed with nutrients: 4/29/30 Fed with plain ph water : 4/30/25 Fed with nutrients: 5/2/25 Fed with ph water: 5/5/25 End of week 5/5/25

25.10.2024: More of the same two more weeks lol

Hello. This is the end of week 6 and the beginning of week 7 of flowering. The plants are looking good. Most of them are showing their sativa side. But plant #3 is looking more like a hybrid. I can see the long THC gland stocks of a sativa but it's growing more bulky than a sativa. Plant #4 is growing big and tall, showing it's sativa side. Plant #5 is the 2nd best in bud size but smells better than plant #3. I see the neighbors big oak tree is starting to shade the greenhouse after 2 pm. It was 29* c as I was taking these pictures in there today. Yes... Plant #1 got a big drink right after I finished taking these pictures. Been busy doing, before winter chores, like, cleaning the chimney and washing the house cleaning up the yard. Putting away stuff.. getting things covered, Cleaning up the veggie garden. Been busy and missed last weeks report. Big year for acorns... just raining out of the oak trees. I can hear big bangs in my neighbors yards too, as the dropping acorns hit things. My driveway is sloped to the street... There must be 200 acorns along the street gutter as they roll down driveway. OK. Take care. Chuck.

Día 69 (01/07) Riego con 1 Litro sólo H20 - ph 6.2 Día 70 (02/07) ¡ Cosecha Banana Purple Punch Auto ! 😁 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Flowering started! So excited! Waiting for the moment when I can get ropes off. LST gives me giant esthetic pleasure)

Last feeding with Top Bud at the beggining of the week, and then 2 weeks irrigating only water The Blueberry started yellowing leaves .. And... look at that beautiful chemicals called chemtrails, which are sprayed from the air, penetrating directly in our lungs, plants and soils If someone needs a proof, well, these strange chemicals look like spiderwebs but if you check it closer and you touch it you know it is not made by an animal, ( and i am even more sure when the plant has like 5 or more spiders around making webs,they are completely different ) it is like shaving gel when you touch it . Got no more to say, better watch..( They still processing loool ) Feeding Schedule Added Micro Vita to the soil again, to clean the roots and help the nutrients break WATER / TOP BLOOM + TOP BUD + TOP CANDY / WATER / TOP BLOOM + TOP CANDY / WATER Song of the week : SIRENS | Z1RENZ - Denzel Curry

Hifi 4G seems to be a bit sensitive to the current and previous feeding levels. Had multiple leaves exhibit nutrient burn. Otherwise all seem to be happy. Hifi 4G, NL/Skunk and Blueberry continue to lead the pack on Bud development. God I hope I don't screw this up!

Start of week 5... Looking back at the first few weeks of veg, i never imagined that they would thrive this good in week 5 already! (Look back at week 1+2 for horror pictures) Whole of week 4 i worked on my climate control automation. I will keep the set up for atleast a week. 👽👉26/9 Big LST day. I got new supplies to manage the rest of the ladies properly. Topped another one ( 5/6 is topped) Cut just a couple of leaves. The crusty ones that did not receive light and the ones casting shadows over my lovely budsights. 👽👉27/9 Waterday* and recovery 2ml/L Bio heaven + fish mix / 6.4 PH 1.68 ECLetting them recover Humidity a bit higher during night because of watering, turned the temp up to match the VPD. 👽👉28/9 They look content, humidity a bit higher during night because of watering. 👽👉29/9 I am happy how you thrive, but they need some pruning/defoliation as soon as they have recovered from the stress training. 👽👉30/9 Did some defoliation, decided to flip to flower after their recovery. 👽👉1/10 They had a case of empty humidifier, resulting in high vpd for 7hours. Water day* 2.1xml/L Bio heaven + fish mix / 6.2 PH 1.75 EC 👽👉2/10 They look great, healthy and happy! Climate is back on point

Week 4 begins for Divine storm 1&2, 4 weeks to go! Both ladies are looking good, DS1 is a bit more thirsty then her sister DS2, I'll keep a close eye on her this week and will feed her extra if necessary. Thanks for stopping by 👽🌳💚

60 días en el proyecto B , empezamos a ver algunos signos que nos dicen que no va a tardar en empezar floración en la variedad 3951 , muy crecidas tambien como el proyecto A , aunque mas a pequeña escala, de estructura muy parecida , y de 11 impares son las hojas mas grandes, a ver cuanto tardan a empezar la floracion y cambiaremos la semana en la tabla de elicitación y nutrición de terranabis a la hora de riego

Last couple of weeks she's been affected by high tent temps, hence all her curled leaves 😓 She is also a big plant in her little 10L fabric pot. I've noticed fabric pots really do dry out faster than airpots. That is a good thing, but you have to watch her drying out too quickly also! I think she'd make a monster plant in a 20L or 40L airpot.😍

420Fastbuds FBT2310/Week 3 What up grow fam. Weekly update for these stunning girls. Up to this point they've been growing picture perfect . Not really doing anything special besides still feeding nutes every other watering 500ml. I'll double the feed this week taking it to 1000ml/1 liter per every other day. Not seeing any burn signs from introducing nutes so will keep that the same strength. All in all Happy Growing

Day 56 : Well 8 full weeks si ce I flipped the lights and this lady looks so ripe now . Her pistils are mostly orange now throughout the plant. I did get a little second burst of pistils befor e the rest browned off and it swelled them very nicely . Her smell is a fruity sweet but there is also another undertone there I just cant grab at. Will hopefully come out in cure a bit more to nail it. She is nice and sticky now too and has some nice clumps of buds to that are solid to squeeze . I plan to loupe her soon to check her trichromes for cloudiness. She has t skipped a beat the whole grow and will be getting another run out soon. Hopefully next update will be harvesting pics !!!. Be safe growmies UPDATE DAY 61:She is done and out . Yay. I had planned and expected an 11 week flowering due to the genetic mix but she had other ideas. She has been a pleasure to try out and grow and will certainly be popping more of the Fruity Gorillas in the near future. She smells very "fresh fruit stall" at the moment and I am looking forward to how she cures up. I will update as soon as she is harvested from the dried stems. I am hoping for a 1p day slower dry but temps here are crazy at the moment and I cant find a cooler space to dry than my grow room yet. Some new pics of her taken out and trimmed. Thanks for coming along for the journey. Results and smoke report soon !

D64. We're at the start of the fourth week of flower, and today was a busy day. First, I had to remove the old lights before swapping in the light from my auto tent. All while trying to avoid trashing the plants in the way too full tent. Not an easy task. The entire mission, with changing the light in the auto tent as well, took a couple of hours. I wish I could keep the Greenception lights in the tent as they're high-quality lights, but the Kingbrite has a much lower profile and thus takes up much less space. Space that I desperately need considering how insanely much these girls stretched. One of the tops on the second girl has unfortunately already been bunt by being too close to the light, and I'm sure I will run into light bleaching and probably foxtailing as the grow progress, but I hope I will be able to pull it off with the Kingbrite light. I also added some wire support to the branches that I super-cropped, as they looked somewhat sad after all the abuse to the canopy while swapping the lights. ------------------------------ D66. It looks like the girls have FINALLY stopped stretching. Phew! Yesterday, I gave each girl 2.5 liters of water @ pH 6.2 with humic acid and EM-1. Today, the green lacewing larvae (Chrysoperla Carnea) arrived, and I dispersed them in the tent to deal with the thrips. ------------------------------ D68. All is well in the tent, but now when the thrips are (hopefully) under control, it's time to address the VPD. It has gotten seriously out of wack due to dropping temps, plus I increased the humidity to make the environment more inhospitable for the thrips. The temps were low due to the light change as the new one is running a lot cooler, but the temps have also dropped outside. The heating in the lungroom has been off until now, so I turned that on and lowered the humidity threshold on my exhaust fan. Finally, I put my small humidifier in the tent, generating extra heat and decreasing the humidity. So far, the VPD has increased from a terrible 0.4-0.5 to an acceptable 0.9, and I'm hoping it will increase further as the ambient RH drops as we move into winter. ------------------------------ D70. We're at the end of the fourth week of flower, and all is well in the tent. The VPD has gone up to 1.0, which is a nice improvement to what it was only a couple of days ago. I haven't scouted yet for thrips, but I will give the lacewings a couple of days to do their thing and then look closer. However, I went to WAR against the spider mites we have in our ornamental plants in the apartment. I sprayed all plants with my homemade pesticide (capsaicin, alcohol, horticultural soap.) since I had it at hand. If that fails to knock the spider mites back, I'll bring out the neem oil instead. The infestations aren't bad (yet), but it would suck to get mites into my tents, so better to be proactive. ------------------------------

with some problems with them 2 ... one of them has fungus again and the other lack of nutrients ... waiting for her evolution to be good ... she gives off a nice smell ... and hoping to try it ... and waiting to see if it takes its purple color

I have a lot to harvest indoor. I pulled over a pound off the plant that produced low quality buds. I put most of my effort in the one grove bag of good good