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JOANNE'S CBD / ROYAL QUEEN SEEDS WEEK #18 OVERALL WEEK #6 FLOWER This week she's doing good growing nice buds and looking healthy she's even getting some trichomes coverage she's a sturdy plant!! Stay Growing!! Thank you for stopping by and taking a look it's much appreciated!! THANK YOU ROYAL QUEEN SEEDS!! ROYAL QUEEN SEEDS / JOANNE'S CBD

CHERRY 🍒 COLA by FASTBUDS Week #11 Overall Week #6 Flower She's getting close to being done maybe 2 weeks max before harvest she's really been a starting plant throughout her grow!! Stay Growing!! FASTBUDS CHERRY 🍒 COLA

Week 9 light schedule switched to 12/12 and bio-bloom & top-max were added to the feed but unfortunately some leaves turned pale yellow and some strange marks appeared. We re going to flush in the next week to make sure feeding can be safely applied again. Autoflower plant going no problem and should be harvested soon. Smell is intense orange with undertones of pine.

Week 4: Started adding Humboldt Secret Golden Tree at 600 ppm. The plant started bushing out from the LST so I defoliated whatever green wasn't getting any light and to expose light to smaller nodes. I was planning on topping this week but to my surprise the plant had created a third axil/node and axillary branch.

Sorry I only have two pictures today but it really isn't much to see. She's still in half veg half bloom mode. Soon she should be loading up. Either way I will be uploading this entire grow journal to my YouTube channel which is easy to remember. I am Ben and I chase big budz. Ben Chasin' Big Budz Hope to see you there

I'm very happy I've already got 107 grams out. I still have 2 plants drying and I'm excited to see how they turn out😊😊💚

For my second Grow i wanted top try something simple so i did nothing to my plants. Ist Just topped one of three after about 7 days.

She is in full flower now and she will have some large tops as she is not a bushy plant.it has been a rainy last week here in my province in Canada but it's calling for lots of heat and sun for next week Keeping my finger cross

Well sliding into another week, I'm checking the trichomes and waiting for them to go milky, not sure if i can just check leaves for that. That's what i have been doing. I've been told i'm waiting for a portion of at least half of the hairs to go an orange/bronze color. Weird blotches become more prominent on leaves that its visible (they are just in some leaves) less than half id say around 15 - 20% and less than half of those seem pretty bad. I would like to know the cause if its nutrient lockout or a disease etc. Or perhaps its normal for late stage flowering i do not know

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Bueno esta semana es la última de vida de nuestras pequeñas, un tamaño reducido y muchas juntas... pero una calidad.. dureza y resina... envidiables.. Esta semana usamos solamente agua con un ph de 6,2 y líquido solo usamos la final solution de b.a.c para limpiar nuestras raíces, al igual que aumentamos la cantidad de agua por riego para ir lavando bien nuestras raices. El olor es muy fuerte pero un aroma apetecible a más no poder... en cuanto abro el armario para los riegos y cuidados sale un olor... que si no fuera por el generador de ozono apestaría la casa!😍😋 En grandes rasgos una variedad de floración relativamente corta.. y con unos resultados impecables.. Hay una media de 5 puntas por planta y algunas incluso más!😍

Enjoyed this grow, easy with no dramas. All were topped grew very vigorous, stretched and filled out nicely. They all displayed differences in growth style but yielded same quality 👌 Cant wait to blaze one down..🔥💥

Elle grandi très bien ! Rien a signaler

GERMINATION WEEK: nowayReggie is super excited as always from breeder freebies. blessed! nowayReggies first time back growing ZIPLOCK SEEDS since ULALENA (check that diary out! https://growdiaries.com/diaries/173907-grow-journal-by-nowayreggie ) Scary Cherrys Lineage: F-{GHOST BREATH} [GHOST OG x MENDO BREATH] M- {CHERRY CHEM} [CHERRY PIE x CHEMDAWG Bx3] 11.27 Have been having success popping the seeds when allowing them to soak for 36 hrs. Also had seeds soaking in a drawer with a heat mat on top set to 80*F 11.29 One seed broke soil but grew sideways 11.30 seed that grew sides...pulled seed that had yet to break ground was pulled(taproot dried out) 5 seeds remain(71%)

Day 71: Hope everything's going well for you. Unfortunately, it's still way too hot here. The outside temperature is 29°C, so it's pretty similar inside the tent. I do have a portable air conditioner, but it would have to run constantly, and that's too expensive for me. That's why I’ve increased the humidity a bit. Otherwise, they look healthy and the buds are getting bigger. I removed two leaves from the Tropical Marker that seemed a bit burned — at least that's what I suspect. Day 74: Today, like every two days, the ladies were watered again with 2 liters of water mixed with fertilizer and Cal-Mag. The girls are looking beautiful. The Tropical Marker is very frosty, but the Purple Kush is also getting frostier. Today, I wedged a board with a hole for the exhaust fan into my window. I hope the room will get a bit cooler now. Additionally, I temporarily hung the lamp’s power supply outside the tent. Day 75: Today, for once, I didn't do anything. Thanks to the new exhaust outlet, the temperature in the tent is actually 5 degrees Celsius lower. The VPD is now back at 1.6. Yesterday, I lowered the exhaust from 80% to 50% to better maintain the 60% humidity, which is working well now. The buds continue to grow, the girls look beautiful, and the smell is getting stronger. Day 76: Today the ladies got 2 liters of water again, this time without any fertilizer. The buds are getting bigger and the girls are looking wonderful. Other than that, I had a little chat with them and then left them in peace again.

Coming along nicely this week, hoping to see them explode in the next 2-3weeks. All are very healthy and have responded just how I like to LST. Purpinator is bringing out the smells in there definately and they are all smelling good in their own way. I recommend all the products I have used in this diary highly as they haven't failed me in the past(yield and quality). The only new product I am using is green planet purpinator, so I will let you know if I recommend this too when all is finished. I'll upload daily or every couple of days. To clarify I have 6x 55litre buckets with 3 plants of the same strain in each. I have used coco substrate and grown all of them from seed(courtesy of barney's farm) Thanks for looking.

Northern lights is looking good. She is growing very vigorously. She has had great growth over the last week. She will be going to her flowering tent in about a week. Everything is going great. Thank you Divine Seeds, and Medic Grow. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Las Ak parece que tienen un problema creo que tiene que ver con CalMag pero no estoy seguro, la otra no está afectada. Bajé la EC pero no se si tengo alguna carencia exceso u otra cosa. El dia 21 subo altura de luces al máximo el día 22 bajo la potencia de los quantum boards de 440w a 350w