En nada empiezan la semana 9. Estoy orgullosos de mis niñas van muy bien😍

High ✌️ also mittlerweile hat sie ein Geruchslevel erreicht, das selbst der 5 mm dicke Aktivkohlefilter es nicht mehr zu 100 % schafft zu filtern. Der gasige Geruch hat sich anscheinend etwas zurückgezogen, mittlerweile riecht sie extrem süß und fruchtig. Es kommt leider im Video und auf den Bildern nicht so rüber, aber sie ist auch extrem verklebt. Ich gebe ihr trotzdem noch ein bisschen Zeit, ich habe das Gefühl, dass es sich lohnen wird 👽🛸 Ich wünsch dir viel Glück und einen guten Rutsch ins neue Jahr 🖤🎆🎇

Final week. Power buds from Plagron helps accelerate the flowering period for sure. I am harvesting tomorrow.😎 This week no fertilizers just water only. Overall happy with the way they shaped up, compared to other strains from other breeders. Need to see after harvest but as a first impression this strain is amazing. The smell now is out of this world. Zesty!!!Lots of citrus scent.Loving it. As for the LST only vs topped. I like the LST only. It matured faster. It is bigger. It has more bud sites . Had an overall better structure. Refer to videos for comparison. But need to see the harvest, weight, smoke report for full comparison Till harvest 👏🎉

*1/30 (Week 2 Flowering) Removed most of the nutrients - Only feeding Lotus (CarboFlush) Raw Npk (Silica) & Element (Flower Fuel) for the last couple weeks before harvet - Running her on 12/12 light cycle - Terps coming in nicely - Buds are getting more and more dense.

Welcome to my Slurircane Diary sponsored by MSNL & Spider-Farmer Week 7, early flip and attempt at reveg into early week 8 but decided I liked the look of her cola potential and decided to throw her into flowering. From the reveg attempt I thought a ratio on/off would be best if it leaned more on the off, so a 13-11 off / on was set for early week 8 and till fattening (when the stretch is over, 'pre flower) This is a bit of a weird one for me, well more a 1st. I'm not 100% sure. But I do remember this getting 3 13.5hr days as did my whole tent. As my cheap timer ran for an extra hrs off when it was meant to be on. So, put it into a flip. And I tried for over a week to reveg, I'm sure if I pushed on. I would of gotten to reveg. (I'm not sure how long it takes, am very pleased though with this spectrum even without the IR that the flip time is quick. So, after a week 7 was finished. I decided to push on to a 13-11 off/on to induce her. I'll increase it to 13-11 on/off in fattening. Sadly has the pre flip and then attempt at reveg make all her nodes very stiff. And near to LST. After pre flower I'll hard defol and attemp to distance the colas. Surprisingly with her been a dom indica her leafs are very Stavia style. Very thin. Would make an ideal candidate for scrog. She is very healthy and been the most responsive with co2/heat. Although growth was stalled from my reveg attempt, I'm sure i cut a good few CM from her Height by reversing it again. I should of looked up how long it takes to reveg 1st. Lessons learnt. I still have an amazing pheno. (I'm a few weeks ahead in Real Time) Defoliation So, this was done lots on this girl. As my plans for her were changed due to poor attention. So, start of wk 7, what I thought was still a few weeks out from me flipping. But, after my abandoned attempt of reveggin. She was in limbo and I decided to risk it with a hard defoliation. (Looks like it was done in pre flower, it wasn't, It was just coming back into veg, but would of taken weeks to get it growing fully again, and at looking I'm happy with it so I pushed again with my Defol to get colas. You can find this on grow weed easy "full tutorial for flowering") am gonna leave her now until she's out of pre flower, she's 35cm or so tall. I don't see putting on much height. As my reveg attempt will for sure hanmer her stretch. Diet Was planning on taking Alga Grow off for the last 10 days r so off grow. So, I only had a few days off it, and its on Alga Bloom now. Thanks to my sponsors from MSNL & SPIDER-FARMER. Just harvested the GranDaddyPurple Auto. (BEST AUTO I'VE EVER GROWN SO FAR, TASTE, SMELL & STRENGHT BEATS ALL PAST AUTOS, BUDS DOWN) will be uploading the weeks on that soon. I've too many diaries. I knew this. So, I plan to do 2 wk updates at a time for 70% of my diaries, so pics and info will be added as those it was a weekly update. Also, I don't put up a harvest wk for a month until the cure. Thanks to my sponsors from MSNL & SPIDER-FARME, look forward to working with your more. And trying a lot more of your products. #convert. Much appreciation to all ppl who stopped by and did your thing. Look forward to seen your work if I know you dropped by of course. Either way. Thanks for stopping by

D43/V39 - 13/05/23 - Changed water , added nutes until EC=0.9 D44/V40 - 14/05/23 - Defolation - Added water EC= 1.1 pH 6.5 D45/F01 - 15/05/23 - Start flowering. Monitoring water from maximum to above, for the week out D46/F02 - 16/05/23 - Continuing LST. Flowering stage is now clear D47/F03 - 17/05/23 - EC= 0.9, Added nutes until EC 1.1. pH 6.4 (added some drops of pH-) D48/F04 - 18/05/23 - Added water and nutes EC=1.1, pH 6.5 D49/F05 - 19/05/23 - Nothing to report

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hi guys So end of week 4 and entering week 5 now. Few issues this week with the feeding of the plants. Dark phoenix are hungry well half of the plants are anyway 🤔🤷‍♂️ so I upped the feed to 1200ppm the dark phoenix didnt mind it as it needed it but my blue cheese suffered and has significant burnt tips on every leaf 😅 I will disconnect this from the auto feeder and flush and add half nutes by hand in a few days. Othere than that the plants buds are growing and getting frosty. Thanks for looking and happy growing guys👌💪💚 Day 37 flower I changed the marshydro tsl2000 to a 400watt hps to finish the job on the scrog along with the bloomplus bp2500 250watt led light. So cheese got burnt quite bad I totally disconnected it from auto pot system and feeding ph 6.5 water only for a week. Dark phoenix is really frosty now still loads of pistils standing on end and filling out now and smells amazing. The next 2 weeks I will keep feeding bloom and top max then I will start the flush on them. Happy growing guys.

Week 3 / Day 15-21 Just added more light and nutrients. Cleaned the system and the filters. In week 4 I’m going to separate them and give them more space with a second box. Have to adjust some things and build it a little bit different for easier maintenance. Conditions of the Grow: 💡 PPFD: 400 umol/m2/s ☀️ DLI: 26 mol/day 🌓 Lightcycle: 18/6 h 💨 Humidity: 55-60 % 🌡️ 🌞Daytime Temp.: 25-26 *C 🌡️ 🌙Nighttime Temp.: 21-22 *C 🌡️🌱Leaf Temperature: 23 *C 🌡️💧Water Temperature: 19-21 *C Water TDS: 600ppm | EC: 1.2 us/cm 🚰Watersource: Rainwater (TDS: 15ppm | EC: 0.03 us/cm Vegetation Equipment: 🔦 Lamp: Pro-Emit DIY-M-KIT 300W 📤Container: DIY 63L Euro-Box with lid Pot: Netpot 2 inch 💦Pump: M.R.S. Standard Whisper Powerpump 8.5 bar 🚿Nozzles: 6x 0.2mm 🫧Waterfilter: 150 microns 🌬️Fan: 2x Clipfan 🌪️Duct-Fan: AC Infinity 100 mm with Controller and no Filter 🍶Nutrient Brand: Terra Aquatica 🔬Sensors: Waterstation (EC, TDS, Temp, PH, Salt), CO2, Air Temp. & Humidity (VPD), Temperature-Sensor Root Container

🌱: Opend up the canopy a little and a light defoliation on day 43 💧 : 2l, 6l SIP, switch to flowering nutrients, reduced the Supermix because of crawling and dark green leaves on Day 44 💡 : Dli: 45 mol/m²/d 🤔: A week to late with the switch to flowering nutrients?

Instagram: MycooS1

Alot of trial and error on this gal and shes fast to spring back, resilient to a lot-- hot res temps, training, etc. beautiful plant and genetics!

Great grow. I experimented with topping and topped to earley which hurt my overall yield but after all was said and done I ended up with just over an ounce of really good top shelf pot!!!

Hello growers Last week for this girl. Going to run her out for 7 days with lights then 2 in darkness. No more water for her she should have enough to finish off the run Swelling around the middle and bottom more so than the tops but she is covered nugs all over. Smell is amazing with 90% cloudy trics with some amber. Leaves are fading and dropping now so she is pretty much done. Next update should be her havest Noticed after all her leaves started to drop the bottom sites are purple/green Love it

Finish the flush with plain distilled water. Got her runoff down to 29 ppm 6.6 ph . Letting her ride it on out while watching the tricombs. a good bit are coudly with a few clear left. What y’all think? a week? Or two?

She was looking sad and droopy before transplant (probably overwatering) but after about 10 days in her new home she’s looking much happier. Getting good growth.

Today is the harvest day , you can see the trichomes are ~50% amber and cloudy it's enough for this 😁

What to say about last week...I learned a lot about what NOT to do next time! the Northern Lights had all the leaves curl up and die on me so I decided to take it out and cut it...fuck it....a bit of a disappointing stick of nothing that prolly wont yield much, I'll add a 'Harvest for it next week when It's finished hanging. I messed up the watering (about once every 3 days) and gave em all (3) cal-mag instead of the overdrive I wanted to give...facepalm.... I guess that finished off the NL for good:P Anyway, the Diesel gals are looking healthy-ish... seeing some heavy leave coloration and curling but buds are looking frostier by the day! nice and compact, sweet smell, checked the trics but still waiting to spot the 1st brown ones, I'll try and keep em cooking till I feel its time (2 more weeks:P) 😁

This week was very good plants look great. Flews are gone🙏 ✌️✌️ Buds grow perfect. I am very surprised, that they go so good. Hope they will stay healthy till the end.