It's Booming 💥 🏆

Día 20 (13/05) Todas las plantas muestran las preflores! Día 21 (14/05) Baño de sol de 2 horas! Día 22 (15/05) N/A Día 23 (16/05) Riego 750 ml H2O + Regulator 0,15 ml/l + CaMg-Boost 0,25 ml/l + Startbooster 0,25 ml/l - pH 6.2 Día 24 (17/05) Las plantas salen al exterior! 😍 Les aplico heno como mulch (acolchado) Día 25 (18/05) Riego 250 ml solo agua todas las plantas Día 26 (19/05) Banana Purple Punch Auto está sedienta! Riego con 750 ml H2O + Regulator 0,15 ml/l + CaMg-Boost 0,25 ml/l + Startbooster 0,25 ml/l + TopBooster 0,2 ml/l- pH 6.2 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

25.07 Watering with 200 - 400 - 400 - 400 Used only 4ml/L TOP-Max (honestly I forgot about the fact, that I have to use the BioBizz Products only once in a week and I already added 4ml before I realized it…) 26.07 my neighbor complained about the smell one store under my balcony (told me he can smell it on the street 50 meters behind my balcony) 28.07 watering 400 - 400

Smells like cheese 😂💚

PH 5.8 EC 1.2 Everything is going very well. This week, defoliation and training were done for the first time. I look forward to any feedback!

Last week went well. Not much happening besides watching her fattening up daily. Had to up cal-mag another.5 mL per gallon because I noticed the start of some minor spots on a couple of the top leaves. She is drinking a decent amount. About a gallon and a half of water every other day. The buds are getting big and I am going to get a decent yield. Until next time happy growing to everyone!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

So I flipped the switch we're now on 12/12 and the stretch is kicking in big time. Plants are very lush growth is aggressive and just simply explosive!! Keep following for the upcoming flower show!!

Willkommen zur dritten Woche der Wachstumsphase.💚🌱 Hallo zusammen! Es ist großartig zu sehen, wie sich die Dinge entwickeln, und ich freue mich, euch auch diese Woche wieder ein Update geben zu können. Heute, an Tag 22, haben meine beiden Runtz-Ladies die Marke von 17–18 cm erreicht und sehen wirklich prächtig aus! Beide entwickeln sich nahezu gleichmäßig und wirken gesund und vital – genau das, was man sich in dieser Phase wünscht. Um den Fokus auf das obere Wachstum und die kräftigeren Triebe zu legen, habe ich die untersten beiden Nodiensätze entfernt, da ich die Erfahrung gemacht habe, dass diese ohnehin keine nennenswerten Ergebnisse liefern. In der letzten Woche habe ich beide Pflanzen zweimal mit jeweils ca. einem Liter destilliertem Wasser gegossen. Mit dem Düngen warte ich noch – damit werde ich erst beginnen, wenn die Damen in die Blütephase übergehen. Ich bin sehr zufrieden mit dem bisherigen Fortschritt und gespannt darauf, wie sich die nächste Woche entwickelt. Ich wünsche allen Teilnehmern weiterhin viel Erfolg und Freude bei diesem großartigen Wettbewerb! Bis bald 🌱👋💚

I started germination of 2 Critical purple kush beans on 29/12/2020. I pre moistened my rockwool cubes with ph balanced water to 6.4. Made sure the plugs were just damp and not soaked. Using a small wooden dowel I increased the size of the plugs pre made holes. Than I sowed my beans into the holes. Ripped off a small piece of rockwool and mulched it up. Lightly filled the holes in with the mulched rockwool. Than stuck the plugs into a misted humidity dome, to complete germination. Shouldn't take anymore than 4-5 days to see some sprouts. Once I see some cotlydon leaves bursting to the surface. I will get the plugs planted into some 1 gallon pots. Plus get these ladies situated into their home. Cant wait! Some Background information on my experience with CPK. I've ran this strain a good dozen times. It's a very short, robust, and branchy variety. Packs on fat dense colas with immaculate bud structre and trichome production. It's a fairly fast finisher and packs a real potent stoned. She gets very sexy purple highlights and hues. Some phenos more purple than others but I've always got color. She doesn't mind lst, and some good training work greatly increases yeilds. I've ran her inside and out and all times of the year. She seems to strive and flourish in any environment. Just give her that light and watch her grow! She packs a very signature kush pallet and she always has a spot in my rotation! Seedsman knocked it out the park with these genetics.

Water week5 26.7.22

Last final defoliation done, now buds are fully exposed to the lights and the best part, harvest trim will be easier. Still it took me around 8 h altogether to defoliate all of them. Tones of buds and frost , amazing smells. Won't rush will give it a bit more to swell.

Final product is been absolutely amazing on these clones - the jungle came out about average but the clones definitely exceeded expectations there absolute fire in comparison but still just as good hopefully I can just improve a few issues we had on this run and dial right in 100% will be so much better, next few run is going to be special I’ve got a few things lined up. From tester packs to breeders packs I can hunt from. So lots to choose from but what to keep to run will be interesting

Week completed, these girls are developing great, the flowers already smell wonderful and are very resinous... Looking forward to the next few weeks. Stay well! 💪🙏

I put the clones into bigger Pots once their roots were fully established. Immediately started LST and put in the clones at an angle so that light is distributed more evenly. Autos also growing well but Panty Punch is lacking because of crippled leaves. I still let her live to see how she develops. I plan to bend the clones in a circular shape so they grow into an even canopy.

She has been doing better then her sister ... pH issue as usual and over watering too , my tent is bigger and I've got the temperature dialed in was at 55 lows and 65 highs for a week which hurt the whole garden a lot .. glad things are better and once it's healthy next week hopefully I'll flip her over to flower and get this show moving along quicker .. thanks for stopping in canna family .. cheers

Day 29! Will slowly start incorporating some nutes or maybe on week 6 Day 30! And day 28’s The leaves are getting really sticky .... -There’s little light burn on a few leaves due to turning up my wattage on my grow lights way to high. 🤦‍♂️🏼dimmed my lights and all good now.🌱🌱🌱☺️ Day 31! Started to do lst all of them with a simple technique. Just a piece of tape... Day 32! Not much to say other than they are loving life Day33! Improved the lst by giving the bottom of the stem some support. I also added my first video 🌱🌱🌱 this strain it’s average height is 3 ~feet Day 34! The low stress training has made them so happy and grow faster. The other 2 are at day 32 Day 35! She is fkn awesome with her two young’s

Loved it!! Relatively easy going!! Couple off moments but not worth getting into but the smell!!! It’s not super strong where you smell it throughout the house when you burp the jars but she has a very (what I could only reference as sour, sweet and lemony, almost like that lemon pledge your mom use to clean the house with) smell but I love it!!!