Still looking smaller than usual but I think it could be down to the genetics I’m not sure, I saw a little brownish/orange burn spot on the tips of Grapey Walter #2, it dosent look like nute burn but not to sure it’s an issue at the moment. Did the first bit of LST of these girls tonight and hopefully they will respond well and start bushing out letting the dident branches catch up.

27/03/21 убрал растение на сутки в темноту перед харвестом. 28/03/21 харвест

she grows outdoor ... without any relevant information ... only her vegetation is slow ... it's autumn ... and the sun is not very strong ...

Start of the new week, some good growth seen.. can't complain, strong vigorous growth in a short space of time. Can't wait to see how quick she grows over the next couple days. Stay tuned. Day 26 - very quick growth from this one, in two minds about letting her go a bit longer in veg, or to flip to 12/12 at the end of week 4. So far so good. Day 27 - so far doing super good. Im due to flip as i cant wait anymore with the other girls needing to be flipped. But i shall see how she does, no doubt ill be running her again. Stay tuned.

Buds are starting to thicken up and get nice healthy looking trichomes. Plant #2 was under a light that did not have the full spectrum setting on. I didn't realize that and now see that it's development is lagging plant #1. We'll see how this affects harvest, but I'm hoping that the timing will still work out.

What up grow family. I hope everyone's New Years is off to a great start. These lovely ladies are showing off for sure. One will get the chop this week and the other two probably by the end of the week. Definitely grew super fast and with not a whole lot of special attention. All in all Happy

Some LSD was done. Pinhas is looking good, giving a nice grow this week.

🌸 Runtz - Week 1 of Flowering 🌸 🌿 Genetics: Gelato x Zkittlez ⏳ Flowering time: 8–9 weeks 🔥 THC: Around 27% 🌈 Effects: A balanced mix of euphoric uplift and deep relaxation, perfect for both creative moments and evening chill. 🍬 Aroma and flavor: Sweet, fruity candy notes with tropical and creamy undertones. This plant has completely stolen my heart! ❤️ The structure is absolutely stunning - beautifully forming flowers and an abundance of side branches make this one truly special. The way it's shaping up already has me excited for the weeks ahead! The first week of flowering went perfectly under great conditions, and I couldn’t be happier with how she looks at this stage. At the start of the week, I performed lollipopping to ensure that lower branches, where less light reaches, don’t take away energy from the top canopy. Now, she can focus all her strength on producing some buds! Grow Conditions (FLO Week 1): 🌞 Light schedule: 12/12 🌱 PPFD: 750 µmol/m²/s 💧 VPD: ~1.2 kPa Let’s see what week 2 brings! 🚀🔥

Привет друзья! Вот и прошла ещё одна неделя. На мой взгляд растение развивается не много медленней, чем положено. Вчера начал кормить, посмотрю на динамику. Всем мира и добра!

450 - PPFD

8/26 Monday morning 12am They are doing good. The camera fell into a plant...so lucky it didn't hurt the plant..that never happened before. My grow is HAUNTED!!!! Dark Devils are doing pretty good. All the others are great. I need more dirt in Blueberry Auto by Seedsman I'll do top filling later this week. 2am got the microscope 👍👍👍👍 I swear she is like a pet and she’s playing dead with her tongue hanging out. It’s hilarious. I’m really enjoying this one. This is gonna be a lot of fun.👻👻👻👍👍👍👍. 11 am I ordered a new microscope!!! 8/27 Dark Devil looks pretty cool. All other plants are doing just great!! 8/28 turned lights up to 52 looking good 4/28 Dark Devil Auto Monster crawls out of Hell!!!! Dark Devil Auto looks like the war boy that figured out Furiosa stole the War Rig. !!!! I put a light tent over Dark Devil monster. I will need to trim the roots and make sure the trays aren't in standing water. Blueberry is in mostly Perlite doesnt take up water the same. New microscope nice picture but no zooming in, I got the kind with the wider mouth, narrow mouth is better getting close to plants.... after 2 years I just NOW figured out the plastic cover comes off?? not the lens cap....im retarded there's just no other way to explain it 8/29 She's ALIVE and wow! got some amazing pics a little blurry and shaky but Dark Devil Auto by Sweetseeds is the coolest thing ive seen. Doing the seed surgery worked. 8/30 Some amazing progress on the Dark DEvil. The others are great Medusa F1 is the strongest followed by Gorilla Strawberry. The lights on the right side of the Mars Hydro light dont seem to be as strong as the ones on the left is ill rotate the plants today. 8/31 5 am lights up to 55 Watts. They are doing good. RSQ Medusa F1 showing the strongest so far, not a surprise there! Fast Buds Straw Gorilla taking off strong next. Pink Kush CBD and Blueberry Auto by Seedsman up next and surviving in almost all Perlite. I will put more dirt in when she gets taller. Dark Devil is going steady and very strong just amazing. 9/1 Growing nice now I will top them all above the 3rd node to keep the grow small

She stretched on me last time. But I was too heavy with the light right away. Gave her light burn. I raised the light and turned it down to 25%. I think she’ll be fine. I’m excited to see how she’s going to do in a 5 gallon!!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Dli around 45 She’s stretching hard and starts to purple slowly . Will follow up with more later