Week 2 of flower is in the books and all 3 girls are looking extremely healthy. Perfect time to start packing on the weight. There are tons if bud sites that will get tons of light due to all the defoliation I've done. I've never defiliated plants this much before and I'm curious to see the difference in bud production below the canopy level. I love trying new things and experimenting with these plants. Always coming up with new ideas on how to do things and making things easier along the way. Can't wait to see how much I'll get off all 3 and see the difference in phenotypes.

This girl is growing like a sword – tall, slender, and cutting through the canopy with style 😅 She's stretched quite a bit, and down below she’s rocking four tiny side shoots that I’m honestly not sure what to think of yet. But hey, I’m letting her do her thing. Stretch seems to be slowing down now, and I'm starting to spot the very first trichomes glistening under the light, always an exciting moment! Let’s see how she continues to shape up in the coming weeks. Staying hopeful!🌱💚

SuperSativaSeedClub PurplePunchOG Auto Week 13 Weekly update on these two amazing ladies. This week I had some temperature swings from cold to hot but all in all they handled it like Champs. I checked the trichs today and only seeing mostly cloudy on the bigger one with still clear and cloudy on the one thats LST. I'll check later this week as I'm shooting for a 90/10 cloudy to amber. All in all Happy Growing.

Late flowering smels is awesome like sweet and spiecy both on melane aroma tricome heads are coloured and ready to harvest

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

hello the plant is still very small, i think the roots gaining on weight and she will be bigger ------> ready for training 😀 she had some problems at the begginning; i think PH problems because of lower quality soil at the top 💀 but the bottom mix is full of organic nutrients so as soon as the roots are big the plant could evolve!!

Great old school kush smell next time I won't nitrogen burn it and she will be huge

July 17th the plants gained a more uniformed color and more importantly my cat loves it too lol

Fed with nutrients on 4/16/25 Watered with 6.8 ph plain water on 4/18/25 Day 5: fade began 4/20/25: fed with nutri

Close to harvest now. Some pistils are still white and I cannot see any amber trichomes yet. Clean water flushing.

Same as last week not much difference in growth pistols are getting more orange color i will be keeping an eye on trichs production in the next week or so.

Week 6 of flower and now I have ugly plants wtf??!!! Calcium deficiency?!!!

Tout ce passe bien, elles continuent à prendre en volume, la Gelonade continue de consommer a max d’eau, la Milky Way a l’air elle d’en avoir de moins en moins besoin. Ça commence à sentir bon 🍋

FBA2508-week 1 of Veg This week has been wild. Once the roots made a foundation the upper growth has been explosive the last few days. Really cool to watch and see the daily growth. I introduce calmag and grow nutrients this week starting at a 1/4 strength. All in all they've responded well showing no burn what so ever. Happy Growing

we pass 6 weeks of flowers. buds are getting fulled and the smell is so so beautiful. she have stain on leavs, i think this si calcium def. Here on pics is day 34 of flowering. On feeding menu is again Iguana Juice Bloom, I spend all Mother earth. it is awesome nutrients. will buy it again.

100% Germination rate for me on this grow.Looking forward to growing this legendary strain by Franco@ Greenhouse Seeds.Grown in south africa

👉Had to throw in some lights on pix for fun. The colors look cool. I use a led work light to highlight parts of the pant and make them show green. The plant is frosty and smelling sweet. Not a strong pungent smell, but a light lemony pine, especially when touched. The lower row of branches has two or three smaller side branches with a few small plump super mega frosty nuggets. At Harvest I will keep track of these and cure them together for comparison. Been Trichome hunting and from what Ive been able to ascertain its mostly clear with some milky and zero amber. Its at the end of the timeline from Growers Choice, 65 days, and it looks like 10+ days to go. Its possible it may not get many Ambers. I will let the plant decide. Right now its still got green leaves, except the scars of the earlier nutrient burn and the calcium deficiency. Have plucked a few under canopy leaves that were droopy and shaded. The upped canopy of leaves are still perked up and the buds slowly plumping up. Very few emerging pistols, 95% are red. 👉The nutrient mix is the"ripen" recipe on the General Hydroponics Flora series, using the full line mixed to make 1.3ec solution. I target the pH to be 5.9. The runoff EC is back to a more predictable climb in ec with no spikes. I rinse fertigated twice using 5.9pH RO water with 1.5mil/gal Calmag only. The pot is saturated at approximately 1.25l. So when I give it 2 liters, I get about .75liters run off. 👉I will begin prepping the dry area for the harvest and get all the jars and BV packs Ill need. I plan on keeping each cola and its good buds together and giving each its own jar. I dont anticipate harvest for another week or two. It wont be fully cured by Christmas, but its definitely going to help make the season brighter!