Missed a few weeks due to I.T. issues. These girls are going great filling out really well, getting some big buds smell sweet/fruity 2phenos are really frosty all are very sticky in week 6 of flower.

All good. Lamp is set to be closer we will see how it helps hopefully.

Eccoci di nuovo qui!!! Super eccitato per questa nuova collab con Exotic Seed, team davvero al top, che mi ha dato l’opportunità di testare questa nuova genetica e di condividere i progressi con tutti voi!!! Come sempre partiamo nei bicchieri per poi travasare.. Questa volta verrà svolto tutto sotto la Lumatek Zeus 465 ProC, mi aspetto molto da questo ciclo!! Non sono cime, SONO CERVELLI!!! Incredibile come questa piccola sia cresciuta, mi ha sorpreso molto in quanto è in un vaso molto piccolo con poca terra ma nonostante tutto ha fatto una crescita devastante!!! Grazie a tutti per il supporto ❤️🍀🔥

Hi guys sorry for a late post this week. So started flushing all the plants now and they all.smell.amazing and getting some nice colours coming thru. Really frosty buds on these autos and really impressed so far.

2 weeks to flower in main tent. Red Hot May have had a little nute burn. Lighting is at 628 on Par meter. 78deg avg. Malt was accidentally super cropped. Healing from splitting her branch. Oozecake aka Judy is my prize now. She fought through some mold 1st week. Transplanted her with only 1 node. Then her 2nd transplant to 1 gallon she was stunted for a week.

Nice terps an frost production , yield is 💩

No huge changes. GPA Cookies are still growing albeit slowly due to the cold. I hope it starts picking up soon. HUGE defoliation on the Chocolate Skunk clone. This girl is responding very well. Money Maker is just doing its thing! I didn’t expect much from this plant, but that just means it has no pressure and can take its time!

I use all 3 General hydroponic nutrients and they are two different pot sizes the Moby Dick is in 30 liter and the blue amnesia is in 18 liters has a very strong odor and blue amnesia is maturing at a faster rate then the Moby Dick. a scrog net is being used in order to give support to the upcoming buds. I am also actually on week 4 I just made a mistake calculating the week. this is my first grow ever. also I'm not sure if using the scroll net it's a good idea for support rather than tying those branches up . I am watering every two days feeding nutes every single time at 900 to 1000 PPM ph at 6.2 the plans have not show any signs of stress but I am worried if it is too much nutrients the plant seems to grow very fast after every watering and seems to be loving it. the blue Amnesia XXL Auto it's growing in soil with 30% Coco and the Moby Dick XXL auto it's growing in hydroponic soil. the seeds were germinated on jiffy's and 100% germination rate plus the genetics seems to me very resilient to heat and everything else including lots of nutrients. the moby Dick XXL auto are the two in the front closest to the camera the two farthest to the back of the tent are the two blue amnesiaXXL auto. today day 31 i did some pruning actually lots of pruning hopefully don't cause stress to the plants or even worse cause them not to bud that nicely regardless the scrog net seems to be working just fine helping them access more light and making it easy for cutting leaves that are no longer needed. 32 yesterday after watering and sleeping today they have show no signs of stress in fact they starting to stretch in the buds that we're not getting sufficient light have start to grow also yesterday from the 16 liters I prepare instead of 3 ml of grow formula I add 6 1/2 to compensate for stress and the need of healing and having new grow stretch. also temperature has dropped dramatically outside making my house cold down from 24 degrees C to 19° at night internal temperature is remaining the same from 30 to 26 C. Day 33 I have been raising the scrog net every day and the light everyday this plant is growing very fast I also notice that where the fan is hitting all the time that plant is bigger I have been moving the fan to other sections of the grow tent to see if any reaction happens and it looks like it makes the plant grow faster . I will not be moving the Scrog net any higher Rather I will introduce another one once they require more support . Day34 notice some of the top leaves are showing a curving down like over-watering without actually over water I think they're showing signs of too much nitrogen .for the next water session I will hold back on nitrogen until I see signs of a fully recovering meaning leafs pointing up. thank you for reading have a happy grow.

Everything seems to be going well. Will top the left branch soon so she has 9 nodes total.

Looking Great this week but I just have I am in need of heavy Phosphorus but for now she’s a beauty

Great climate, ideal. After careful consideration, valued the considerable size of the plants, the flowering stage and the yellowing of the bottom canopy , I decided to feed one last time with veg nutes at half dose. This will be followed by 2 waterings of water only and then a boost of flowering nutrients. I love this strain. She's blooming very well, quickly just like she was growing indoor. For a couple of days it has been emitting an extraordinary citron/diesel smell and now becomes to be sticky. Expected harvest time: 3rd/4th week of September. Thanks for following, I ❤️ you

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Got a dehumidifier it’s reading different from what mine controller 69 is saying but I have it set at 40% it’s the lowest so it’s going pull humidity regardless right now it’s about 53% which is okay would like it to get down to the 40’s I also changed the light schedule today to 20/4 the humidity raises at in my tent when lights are out so I figured change it to 20/4 instead of the 18/6 I’ve been running so if humidity does go over it won’t be for as long as before hopefully this works just trying to avoid any mold situation So far the Amneisa Haze 2 is at stretching she’s going be big I can but the haze 2 is done seems to just be flowering the sour diesel is looking good as well so far I’m happy with its looking like left town for two days and the auto watering pots worked!