So last week I accidentally posted pictures of the other phenotype of gorilla cookies in this diary. She's doing great though. By far the biggest plant in my tent currently. Feeding with med man brand nutes, kind bud base at 600 ppm and half a g of bloom per l of water

Week 2 is progressing smoothly. While she’s not quite as strong as her box mates, you never know—Messi was a small kid once too! I'm giving her the full Canna nutrient suite, keeping the pH steady at 5.8, and watering every 3-4 days. No major issues so far, and everything's on track. Next week, I’ll start with some LST to encourage better growth.

Here they are, finished! The plant in the smaller tent definitely packed on much more weight. The issue with the other plant was being in a 12/12 tent and having a taller plant in there which was hogging the light. Still got beautiful smelling flowers. These were all taken in the last week of flowering. Super happy with the new setup that I put in with the Mars Hydro light! Very nice plants but they did take their sweet time in the beginning.

Día 13/10/20 día 37 de floración Nuestras nenas continúan estables, el único cambio es la bajada de temperaturas, sobretodo de noche aunque con la ayuda de un calefactor la mantenemos en 23 grados. Poco a poco los días pasan y se va a cercando el lavado de raíces, por lo que ya no subiremos demasiado la ec de las niñas, no te lo pierdas!! Día 15/10/20 día 39 de floración Continuamos vigilando las nenas, hoy ha tocado una inspección detenida y un aspirado de la sala para minimizar riesgos. Por otro lado la nutrición sin cambios, sigo con el riego por goteo programado para riegos de 1,5 min cada. 6 horas durante el día. Ahora solo falta seguir controlando a espera de cosechar los resultados, te lo vas a perder?? Día 17/10/20 día 41 de floración Hoy rellenamos el tanque de nutrición, al final se queda con 1.85 ec y 6.1 de ph, esta será la ec más elevada que utilizaremos en nuestro cultivo. La mantendremos así durante unos días hasta que toque hacer el lavado de raíces para asegurarnos de cosechas unas flores con un aroma y sabor sin contaminantes (algo de lo que ya nos aseguramos desde un principio al utilizar zyco)

🌿 week 7 Update: Frost & Fire! ❄️🔥 We are officially moving into the "sweet spot" of the flowering phase! The tent is starting to smell incredible, and the girls are putting in some serious work. If you look closely at those bud sites, the trichome production is kicking into high gear, giving everything that beautiful, frosty shimmer we’ve been waiting for. The Feeding Strategy This week, I’m leaning heavily into the bloom boosters to ensure these colas have everything they need to swell up. Here is the current cocktail: Bio-Bloom (BioBizz): 2 ml/L – Keeping those base flowering nutrients steady. Top-Max (BioBizz): 1 ml/L – Stimulating cell division and increasing sugar production. PK 13-14 (Plagron): 2 ml/L – The "heavy hitter" for this stage to add some serious density. 📈 Progress Report The Stretch: It looks like the vertical stretch has finally stabilized, and the plants are now focusing 100% of their energy on bud development. Canopy Check: I’ve been performing some light defoliation to make sure light is reaching the lower bud sites. Airflow is key right now to prevent any moisture buildup in those thickening clusters. Flower Development: The pistils are still mostly white and reaching out, meaning we still have plenty of swelling left to do. Grower’s Note: Keep an eye on the leaf tips! With the addition of the PK 13-14, I'm watching closely for any signs of nutrient burn, but so far, the girls are drinking it up like champs. 📸 What’s Next? The plan for the coming days is to maintain a steady environment and keep the pH dialed in. I’ll be watching the buds get thicker and stickier by the hour.

Jo

Been slacking at updating this. Putting in a lot of OT at work and the diary has fallen to the wayside for a bit. Had to put the trellis netting up. The two Lemon Haze plants are getting chunky and falling over under their own weight. Looks like they only have a couple weeks left where as the two Grandaddy Purp on the left side look more like they have 3 to 4 more to go. Hard to tell as they don't seem to be swelling up as much as the other two. Getting so close I can SMELLLLL IT!!

Ladies and gentlemen today is day 49 for our Kannabia runtz and she is looking good... It's nice to grow a plant without any training sometimes they get done so much faster.. this girl, I think is done stretching and is now going to start producing buds which she has already begun doing... Thank you and big shout out to Kannabia seeds for making this grow possible.. Thank you to everyone that stops by.I hope everyone is doing well.God bless and happy growing ✌️. PS.. i'm using also maxibloom .5 tsp with general hydroponics Silica. 5 tsp ..

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Last watering before harvest, I'm planning on chopping her down Wednesday! Buds looks so full and smells wonderful! Very excited to harvest this strain

04/26/24. Orchids. Going into week 6. I have #1 just where I want her. #2 isn’t exactly how I’d like but much MUCH better than where she was. A little uneven still, but I can’t complain. She’s still VERY dark and I’m emphasizing an issue on her leaf which I still haven’t figured out if it was caused from nutrient burn or ph. Either way, still haven’t fed either girls since the tea ONE time awhile back. #1 has lightened up a LOT and looks about right to me now. Transplanted both on the 21st, I believe it was. I’m not sure if I’ll flip them to flower now or wait until they’re 8 weeks. Guess we’ll see. Until next time

Its over, thank you for this plant anesia it helps a lot at Parkinson disease symptoms!

Been an awesome week!😎 Lots of things going on last week.. We started LST, made a few LST adjustments, gave the ladies a haircut, and further refined the nutes👍 Looking forward to seeing some signs of flowering this week. 🙏 I used to believe I was a man of patience 😅 Stay tuned for pics and videos throughout the week.🙌 Happy Growing! 🤙💚💪🌱😎🌱💪💚🤙

I think she's growing into flower stage .. not sure when it technically starts. She's not as big as I thought she would be when flowering started but I think she can still grow during flower stage. She is kinda bushy, that's pretty awesome. She's really a lovely plant all by herself.. even without the fact that she will one day get me stoned. She really is lovely. I haven't started giving her nutrients yet because she just doesn't seem to need them. I attempted some low stress training and broke a limb .. so now she's super lopsided lol .. poor girl. I've messed her up so many times. I'm trying to see if I can get the limb to grow as a close. 12/19 The soil seems to be giving them enough nuts at the moment... I noticed her tips were coming in yellow but that's something that happens every bit of now growth and water. I'm not putting nutes in her water yet. I'm not sure when I will since the soil is doing such a good job. I'm thinking she's going to flower soon but I'm not sure. I might have the wind too close to her but it seems to help keep the little bugs away and let's me worry a lot less about humidity. I turn her but I like to have her toward the wind on that cup shape. I used black yarn to tie her down and recently switched the pipe cleaner .. much better. Also using the binder clips to the sides of the bag to help have lots of places to bend. I'm currently tucking in the big fan leaves to try and keep them out of the way, but still letting them be big solar plates .. getting that light to help the plant grow and heal. This poor plant has gone through hell and back. She is my first grow and has lived through every mistake I've made .. and thrived! I love this girl. When she was a seedling I tugged her tap root When she was a small plant I accidently yanked her roots When she was a seedling first repotted I dropped the pot behind a shelf and it was everywhere I started with no real light except Amazon deals I broke half of her area off by breaking a big limb

So, first of all, I want to say that I´ll be doing the diaries in a weekly updates way, instead of day by day, not only because it is less heavy on me, but I think it´s a better and more attractive reading. So, tell me if you prefer day by day or weekly updates please! Thanks! So, this week was pretty good, thricomes are looking pretty close to harvest. About 90% cloudy, with a little amber and clear. Watered with regular water and didn´t feed her. Flushed it with regular water on day 74, looked like it had still lots of nutes/salts buildup. And about flushing: I saw a really good study (I can link if it´s of interest for someone) showing that flushing isn´t really required, specially if you´re doing organic grows. It was really well explained and had solid arguments and comparisons, even tho, I flushed her in order to see if it really makes a difference. On day 76 when i first opened the tent in the day, she was all the way to the floor, almost hitting 2 tiny girls on the tent. Since she doesn´t had any strenght to support herself, I used some ropes to mantain her up. In resume: The week´s been really nice, she´s beautiful as hell with some purples and I´ve learnt some really good stuff as well.

This one was Baked in Paris by PerfectTreeSeeds, grown with GreenPlanetNutrients only! Check the other weeks to see the ones with AptusPlantTech! Great zkittles terpz, awesome structure, beautiful colours. So, this is the one I liked the most since day one, love her colours, her structure and her smell, but precisely because of that I was too excited to harvest her that I forgot to take proper photos so I leave the ones of her last days .. Will try to update soon with some pictures of her on the drying screen ahah

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

The plant took off this week. I am relatively new so was working through getting my PH measuring/balancing down. Now that I have established a better method the plant responded well. Put in a scrog to help even the canopy for the future. The other plant I have running is a bubblegum autoflower that is a few weeks older (right). Personally I really loved the look of the gold leaf at this point, it had a very nice spiral look to it almost like an xmas wreath.

Ran in to a few problems/issues