Day 67. Buds have become fat. Trichomes ah becoming visible clear. Fresh reservoir change out yesterday.

One of these is killin it, the tallest plant in the video is #3 it's almost 4 feet. The other ones are doing good too but the big one is impressive. I kept a cut of each I'll prob run them before these even finish. Added some tnb co2 for a boost.

Day 44-21/05/22 everything is looking good I might take them out tomorrow do some light defoliation and move them about abit not sure yet!!!! . Day 47-24/05/22 they look real good today!!!!

exelentw

Week 1 Aiming for: Generative steering VPD 1.18 DLI=44 37-53luxx Day 3 Irrigation system did not produce runoff. I will hand water them all untill runoff. Dripper uses 4.224gl per day Plants are 14-18inch tall (4 minutes is a shot)2%= 220ml 4minutes to hit 220 8.64*110=950 4 events of 220=880 Early flower schedule (Generative 4 minute water) 6:30 220 P1 6:49 440 P1 7:09 660 P1 7:24 880 P1 7:43 1000 P1 03:00 1220 P3 36.8klux(40.8DLI)is minimum(940umol) 40klux (44.37DLI) is Perfect(1027umol) 53.2klux(59.18DLI) is maximum (1370umol) They say PPFD of at least 800-1100 (800-1100) micromoles/m2/s-1 for perfect optimal lighting without additional CO2. PPFD of at least 800-1500 (800-1500) micromoles/m2/s-1 for perfect optimal lighting WITH additional CO2 Day 6 Plants are good for LST Day 7 looking like we need to defoliate. 4 plants still look sick WAtering is going well

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Wir befinden uns jetzt in der 7 Woche sie hat deutlich nochmal aufgebaut bin sehr dran interessiert wie das Endresultat ist. Bisher haben sie schon leichte Lila spuren an den Buds hoffe das wird noch mehr. Soweit bin so zufrieden auch wenn ich denke sie sei zu klein.

The plant looks absolutely gorgeous, let's do some mailing on this lady as well to avoid a long height plant that cab be seen from anywhere, I'll try to make a beautiful bush full of fat colas, let's see what we can do guys!!

Day 35: The weeks seem to be flashing past now we have some buds to admire. Wedding Cake is storming the field in this tank and without doubt the leader of the pack. She feeds really well and shows no sign of slowing down at all. she is beginning to give of a really nice biscuit smell up close and touched and her sticky buds are certainly pushing oils out. She has a good deal of space to keep growing out and up into so heres to a 5-tier wedding cake at harvest. Black Ghost O.G is certainly a tryer and is putting some very frosty trichs and pistils out now to make up for her lack of size. I dont think it is an impressive grower but i dont doubt the hit that she will give from the building up of these trichs . Gorilla Glue#4 is being a typical GG#4 I am happy to say. she is falling under her oily buds weight on her skinny limbs and need a pinochio conversion to keep her upright so i got bits with the yo-yo's to keep her up. She smells so nice when touched but is so sticky she doesnt want to let go !. Now that her roots are not submerged entirely , her leaf colour has come back to a beautiful green again. She has a fairly decent size but I think quality over quantity will be a mantra I use for her yield. . Have a fun week until next time . keep it green and mean.

Привет друзья. Моей растихе сегодня 28 дней. Начал применять LST технику на 19 дне и продалжаю применять её через день, а 18 августа добавил ДЕФОЛИЗАЦИЮ С 20.08 ДЕФОЛИЗАЦИЮ делаю каждые 3,4 дня С 20.08 применяю технику LST каждые 3,4 дня На сегодняшний день влажность 63% Растение растёт хорошо. генетика радует. Всем мира и добра! #Smail_Seeds

MIMOSA ZKITTLEZ FF / GANJA FARMER WEEK #13 OVERALL Week #3 FLOWER This week she's done stretching just in time she's almost touching the light. She's starting to fill in and get some weight to her buds additionally you can see the trichomes starting to form on her buds! She's absolutely starting to smell good also! Stay Growing!! Thank you for stopping by and taking a look it's much appreciated!! THANK YOU GANJA FARMER!! BUDTRAINER.COM BUD CLIPS MIMOSA ZKITTLEZ FF / GANJA FARMER

Day 43 this orange is running absolutely faster than the last I did. Every day flowers are getting bigggers and resin is awful to be at 42 days. I’m really loving aptus product, but I really can’t ell what’s is pushing her so fast. Behind, in the video, you can see another auto is a strawberry banana and germinated with just 24 h difference but is starting just now to get into flowering, same soil ‘, same feeding.. incredible Let’s see what s happen

We are finish almost.

Day 1 Kinda fattening up now. I guess I'm getting close to the end now. No feeding so far. Trichs are still mostly clear on the buds (with some amber and cloudy in between) so I'll need to wait some more for the flush but it won't be long... let's see... Nutrient values above are still at 100% from the last feed but next feed I'll back down for sure. I'll go 75% next time, maybe 50%, maybe 0% and I'll flush depending on how she gets along...

Day 14 and the girls are looking good. No deficiencies. Everything seems to be fine.

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 65 since time change to 12/12 h. Hey guys :-) We arrived in the last days before the harvest 😊. The buds have swollen again the last few days. The trichomes are 90% milky and 10% amber 👍. This week it was poured twice with 1.2 l each time (for nutrients, see table above). As always, it is put in the darkroom for 48 hours before it is harvested. There is still a harvest update and a final update after fermentation 👍. Until then, I wish you all a lot of fun with the update and stay healthy 🙏🏻 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy this Strain at : https://sweetseeds.es/de/cream-caramel/ Type: Cream Caramel ☝️🏼 Genetics: Blue Black x Maple Leaf Indica x White Rhino 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4

12/8 - Here we are at the beginning of week 10. Her buds are starting to frost up and she is definitely bulking up more than her sister, but her sister has a longer projected flowering time-frame (Heavy Sativa vs Indica). Anyways, did some minor pruning for airflow optimization and to remove some unhappy leaves from her undergrowth. They weren't yellow, but they weren't getting enough light and it was showing. Haven't done much to her this week other than top her off with Tap every other day. Seems like her feed from last week was on point as her pH and EC were solid as the water level lowered. After her first top-off, her EC started to fall as she ate more (reflected in the lower ec in the res after topping off with tap). Tapered her down to 80% strength as she is moving further along in flower and should hopefully finish sooner than her sister. If she needs a bump in her EC, I may top off with full strength nutes, but I'm going to just wait and see how she responds. Breeder's timeline reflects a ~65 day seed-to-harvest, but I can assure anyone out there growing this strain in DWC that she will not be ready in that time-frame. Currently she is sitting on day 67 and still looks like she has another 2 weeks or more. I grew her in soil before and she was ready pretty darn close to her expected time-frame (~70 days), but this strain responds to DWC differently. She didn't have slow growth or anything; just seems like she will veg longer in hydro than in soil. Not sure why. Thanks for stopping by the garden, and, as always, Happy Growing!

This strain smell is best. It's sweety orange smell and the buds looks nice and frosty

Ok