This girl is looking delicious! She’s not packing it on quite as much as the Obi-wan’s are, but they are so beautiful looking. Just covered in tricombs everywhere. She is starting to show signs of pk deficiency, older leaves are starting to yellow and we are now seeing the hairs starting to recede and some turning red. Probably be harvesting her first before the other 3 obi-wan girls as she looks to be slowing down faster...if that makes any sense...lol I’m not planning on doing a lengthy flush on her so I’m thinking we will wait till we see all cloudy tricombs and then give her a week or so flush till we start seeing about 20% amber. Well that’s it for this week! Cheers 💨

Start of week 6!! One more week until flip All pits have been brought back up to ph range in the 6s

Guys, it's week 8 and the Purple seem to be ready to be at the en of flowering stage... I am really excited, but so afraid of doing a mistake... I Will now flush her for 1 week of two maybe depending on me receiving my microscope on time! For the amazing master KUSH, it is going well for the big one, budding seriously. Her Lil sis is not going that well we'll see! Enjoy my little video, if you like it please let me know and hit the like button :) Love and peace ✌️✌️✌️

[ Information ] For all grow information, including strain and room details, please see the first week of veg. [ Updates ] (Flower) Day 1 - Light intensity increased to 65%. C02 increased to 1300ppm average. Day temp/humidity 85/70 (1-1.2VPD), night temp/humidity 75/65 (.8-1VPD). Fed a diluted compost tea mixture before lights on. Base water was R/O and tap water mixed lightly with silica and Tribus microbes. Tea was a mixture of Fish Hydrolysate, Bat Guano, Molasses, Fulvic/Humic, Kelp, and Earthworm Castings. 8 gallons of tea were added to 72 gallons of water mixture for an 80gal batch total. The batch was mixed for a half hour before feeding to the room. I did not PH or PPM test the mixture, organic material is hard to get an accurate PPM reading so the numbers are useless to me. I will be working to bring the lights to 100% power over this first week of flower. Day 4 - Light intensity increased daily, currently at 90%. Will raise to full power tomorrow. C02 increased to 1800ppm average. Temp and humidity for day and night are still the same. Lights were raised slightly to maintain 12 inches from canopy height, and a few growth nodes that were above the canopy got topped. Watered today with an 80gal (1.25gal p/pot) mixture, 10% tap 90% r/o water. 6.8 PH, 2.2ec. Foliar sprayed yesterday before lights off with a neem mixture for weekly IPM. Canopies are stretching relatively evenly, though I will be adding in support nets within a few days to help maintain the even spread. I've run this strain before so I'm fairly confident that I know what to expect during these few stretch weeks. Day 7 - Lights have been at 100% since day 5. C02 still 1800-2000ppm on average. Plants are stretching quickly into the lights, I have yet to readjust their height. The best growth usually happens when I do nothing, and I've done almost nothing the past couple days besides enjoy the unusually warm spring weather my area is currently experiencing. Watered today, 100gal (1.5gal p/pot) mixture, <1ec. Mainly an organic feeding for microbe health, also wanted a bit more runoff than normal due to the high ec feeding previously.

Ar first when she sprouted grows slowly and in early veg i made many stress and techniques in little pot before transplanted in big pot she grow really faster and get many inside stems whick i cut them all and stay only big n top ones it was very hard to make lst and somethimes i needed to make hst her stems are wildly hard as steel and not easy to give her nice bodybuilding she had many wound and cuts on stem. Flowering phase was amazing beautiful at first it was full white stretching so long middle stems nonstop growing whole 6week and only at last weeks she get many trichomes flavors and starting yellow pistils visually looked pineapple

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Easy for grow, If you haven't tried this strain. You should hurry and buy the seeds. A link for buy 👉🏻( https://2fast4buds.com/ )

Buenas a tod@s... Bueno otra semanita más para las niñas... Las gorilla zkittlez... De momento va todo bien, todo en orden, las eh pasado a la maceta de 7 lt para q ya estén ahí, un buen sustrato y un poquitito de nutrientes para las raíces... A esta altura no podemos hacer mas q darles cariño y dedicación... De momento esto, luego más y mejor, a seguir trabajando duro... 💪🏻💪🏻 Buenos humos para tod@s... 😎⚕️💪🏻💪🏻 🇦🇷🤝🏻🇪🇦

been a decent week for jorges diamonds shes having a good stretch now branches getting thicker nice tight node spacing will be removing calmag and silicon this week giving a flush and then start with some buddy thanks for reading happy growing guys

Thanks to the folks on here for the tips on HST to deal with the vigorous growth and the plant tops getting too close to the light! Watched a couple of YT vidz that explained it well and the HST I did to bend the stems away from the light so i could use the SCROG net worked well and the bendy stems firmed back up in no time. Very subtle smell so far, the leaves I'm pruning from the base of the plant smell great when ripped apart between the fingers. I might stick to Autos for subsequent grows as I just don't have the tent space to deal with tall Photo-period plants. Watering/Feeding about twice a week, growing really well and really strong plants.

La più piccolina di tutte la Cherry ma anche lei super cime compatte ed odore fuori dal normale, in attesa di essere secca e pesata anche lei, resina ovunque anche per lei, non vedo l'ora di fumaria, nonostante attaccata dalle mosche bianche per prima ha resistito molto bene alle avversità esterne. Grazie fast buds, grazie amici che mi supportate e a presto per migliori aggiornamenti!

Gushers has been growing great under the Spider Farmer G5000/UVR40 lights, in the Athena blended line nutrition. She had her canopy lollipopped today. She also had a selective defoliation done. She is showing her first pistils as well. Everything is going well. Thank you Spider Farmer, Athena, and Royal Queen Seeds 👸 🤜🏻🤛🏻🌱🌱🌱 Discount code:GROWERS20 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Spider Farmer Official Website Links: US&Worldwide: https://www.spider-farmer.com CA: https://spiderfarmer.ca UK: https://spiderfarmer.co.uk EU: https://spiderfarmer.eu AU: https://spiderfarmer.com.au G5000 Light Amazon Link: amzn.to/4643esa UVR 40: https://www.amazon.com/dp/B0BR7SGTHS Discount code: saveurcash (Stackable)

Flush is on!! Now we wait on a fade 😊

We did really well this round with this strain even though she grew very different from what Im used too. We ended up with a half pound of some excellent smelling and tasting cannabis!

We're almost there. There're some amber trichomes, just a few. I'm gonna wait a few more days to harvest it. It smells nice! :) And there's sign of nuts deficiency, and some light purple tones.

So this grow came to a finshed but I had a. Few issues that’s held me up from finishing up the diary, but in due time I will reupload it all in order soon. But over all I will also update with a final weigh in of each strain produced so stay tuned 🤙🏻🔥

🤩🤩