Welcome to Auto Alaskan Purple Grow by PoshGrow! 🍀 Week #10 2020 September 24th. - October 1st. General Info: When planted: 2020 July 23rd. Week: 10 Days: 63 - 70 Last Update Day: 2020 September 30th. Plants: 5 Alaskan Purple Auto. Equipment: Tent: MarsHydro 1mx1mx2m or 39"x39"x72". Light: HLG 260w V2 Rspec QB Kit. Exhaust: 4" 322 CFM fan + Viper Carbon Filter. Intake: 4" 100 CFM Inline Fan. Oscillating Fan: Lower: 4" Ram Fan. Upper: 9" Voxon Box Fan. Humidifier: Taotronics TT-AH001. Dehumidifier: Pavlit MD750. Soil: NPK soil 40% Compost, 50% Peat Moss, 10% Agroperlite. Pot: 7 gallon Fabric Pot x 5pcs. Nutriens: Fox Farm Trio. PH Correcton: Chemoform pH-Minus Granulat. PH Pen: Cheap Chinese one, I callibrate it every time I use it. Water pump: Digital microscope: cheap Chinese USB X4, 1600X. Comment Removing all popcorn buds really pays off. Buds getting bigger and fattier every day. Right down corner girl will be done fastier then other ones, I think shes going down upcoming week! Ill start checking trichomes with digital microscope. Also this week was the last with nutritions, from now on, going to use only pH corrected water to flush those beautiful girls😉✌️ Peace!

Kicking ass this cheese went outside and brought back inside

Wedding Cake was pretty easy and fast to trim. ! bud weight = dry after 2 weeks

These lady's have been taking there sweet time initially wat I thought was going to be a ugly one turned into the best and most frosty one I got in tent although none of my ggxwws look the same but two but this strain has grown very good and has one hell of a smell to them...well this week was going good but had one turn hermi on me lucky I caught before it got bad and got it removed before it pollinated my others

Willkommen Wie bereits angekündigt wurden die beiden Damen umgetopft. Auch Secret Source hat sich gut gemacht. Wir sind sehr stolz auf die Kämpferin 😇🍀💚 Ansonsten werden sie auch im moment durch die neue selbstangereicherte Erde nur mit angepassten Wasser was den PH Wert betrifft gegossen. Auch hier werden wir zeitnah noch etwas entlauben. Die Nahrungseinstellung wird hier noch mal sehr interessant 😅 Na gut, haben sich alle samt lange genug ausgeruht. Auch wir grins 😬 So wir wünschen noch eine angenehme Woche wie auch viel Erfolg und falls, vielen Dank fürs vorbeischauen! VG ✌️ 😎

@twenty20mendocino Ateam R&D Update ~ Lets Go day 38 of flower an these ladies are looking amazing! Gettin in some nice frost a the smell is insane, so many different terps Sour , skunky , fuel , gassy funk a berries 😍so amazing! Not too much longer to go a these ladies will be ready for flush , but let’s take it day by day!! Hope y’all enjoy an have an amazing productive day ! Cheers to y’all 😶‍🌫️💨💨💨💨

By the start of the week I’ve noticed magnesium deficiency on plant A and B, so I started adding Biobizz Calmag on Froglord’s kind recommendation. Otherwise I started slow defoliation process and I’m currently removing around two leaves per day. Plants definitely grew larger than expected but I’m not mad :D. They’re taking a bit longer, but it should be worth it. I’m expecting harvest on plants C and D soon.

Week 11 I though she was gonna be done this week only gave her RO water for the last 2 weeks but looking through the loop her trichomes are still mostly clear so I think I'll give her one more week. She looking great, smelling great, buds look amazing. Tried to get some good video so you can see how lovely she is!!

Wow, what a week of dank?It's been. I haven't having a blast.I'll tell you what. I'm super grateful and so glad to be here. Learning and enjoying the processes and steps as it goes. Understanding the differences between phenotypes of same plant within the progeny. Did dolphin wax it's glossing. She's definitely a keeper, and i've got a couple clones of her. All love and gratitude 🙏 check out beanpatchseeds.com if you havnt already. More fire on their site 🔥 appreciate the support. Happy growing yall

25/06/2021 (F DAY 28) Se agregó unos 5L de comida en modo top dress. Se agregó harina de todo tipo, humus, compost, microkashi, bokashi, guano, etc. La flora viene muy bien y los olores son muy intensos y sabrosos. Sweet Cheese: Posee el desarrollo de flora mas rápido, lleno de tricomas y con un aroma intenso a queso. Black Afghalien: Posee un característica muy particular de pistilos super finos, el olor a incienso con resinas es increíble. El color característico negro de la black está apareciendo al mes de flora con el descenso de la temperatura. Pomelo Haze: Es la flora más lenta pero sin duda alguna es uno de los mejores terrenos que poseo en esta carpa. Tiene bastante distancia internodal y muy poca ramificación.

she is 70% amber ... in a few days she will be cu...I AM HAPPY by the smell of her and her color in the flowers of this automatic ...

Hello. This is the end of week 5 and the beginning of week 6 of flowering. Showed around my yard a bit. Lots of nectar giving plants and bushes for the humming bird's, butterfly's and bee's. Smells wonderful in here With the LemonPaya and the RainBow Melon in here it smells like tropical skunk. Yum. The plants are growing well and everything seems good. The buds are bulking up and getting sticky. OK. Be Great. Chuck.

stoked to see the effects of the pk booster, the overall growth seems quite dense and stacked for a 80% sativa autoflower. Lowering CogR Flores to 3ml/l in anticipation of a harvest within the next 3-4 weeks. The Smell is getting more intense now and is not as a flat anymore. Nice and juicy Sweetness, some skunk and hints of chocolate or candy. Mixed 10l of Nutemix with the Redboom a couple of days ago, mixed another 10l todays and once the 10l are gone i will stop using it until next run - so a total of 20l for those 2 plants, ph regulated water once inbetween both applications

Finally, I found the time to finish the report. Thanks for joining me!

She Is a beautiful babyyy. Growing so fast, and She love lst and topping. I think, She will give me some beautiful flowers 😍

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50 day - 63 day pure water ph 5.9-6.3 52 day - Deleted one lamp 240wt

10/5: Comparing photos from the beginning of last week and today, it was pretty decent growth. If they were stunted from being in the temporary grow bags for too long, it wasn't too badly. --Blackberry I (I call her Halle), is really purpling up well, and looking like she'll be a single uber-phat cola...no branching at all..whereas her sister is all about the branches. --Wedding Cheesecakes are consistently big and vigorous. They'll probably be the best producers of the crop. --Orange Sherbets look like clones they are so consistent in shape and leaf structures. Kinda short compared to the other varieties, but nice and bushy. --Gorilla Cookies are also identical in plant shape, but two of them are a few days ahead of the other as far as pistil production goes. Also not a very tall cultivar, but many branches. --The late/stunted Lemon Pie is praying to the sun at all times and starting to look like she belongs in the same crop. The bigger LP is gorgeous..perfect structure, the other one is catching up, but my cat keeps defoliating her.😏 ---Strawberry Pies have massive sun-blocking fan leaves that require constant tucking, but they are branching well, and a couple of them are pretty beefy. The other SP is doing fine considering her crooked trunk and slow start. 10/6: Today I noticed 9 "lone" nanners on the nodes of the crooked trunk-Strawberry Pie!😧 No clusters.. just single pollen sacs here and there.🤨 These plants have experienced no major stress at all...I'm keeping it like late-August upper-peninsula Michigan in the closet. 80f/50%RH 👌 Damnit...this is a new, stable strain, right??👈 I've seen no other SP diaries with hermaphrodites...just my fukt luck I guess.😖 I plucked them and will watch for more..and I guess I'd better keep a close eye on the other two SPs as well... I just got my wife a new 16 x 7 x 7 greenhouse frame with the green semi-translucent cover for her 4' x 12' raised bed...if i see any more sneaky nanners appear, I'll probably remove the plant(s) and move them outdoors to finish in there. I swore I'd not do any training this run, but while removing the pollen sacs I couldn't resist opening up and tieing down some branches on the hermie.🤷 10/9: I fed them.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. 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