Buds looking really good, the trichomes are almost ready one more week to harvest

04/01/21 Amazing colours have came through during cure showing off her red and purple! And she’s frosty all over and tastes like a tropical dream!mango pineapple tones with a side of lime! Would defiantly recomiod Update; colours are coming through got some amazing hues just uploaded some vids 😍 Got the green pheno on both???! But it’s but either way I love this strain tastes a lot like the black cream auto I’ve done of sweet seeds too. So many colours in the bud and the flavour is immense throughout! Tastes like tropical mango and slight pineapple with a lovely pungent limeish taste it’s so nice and the high sent me to sleep when I tested the result! Just had to lay there and close my eyes and enjoy it and woke up to the end of my film ahahah would defiantly recommend Anyone growing very easy and amazing results!

Ok so I did a drip system on these plants for about a couple weeks took it out now half the plants want to be watered more then usuall and if not they start to droop other then that it’s fine hopefully they will do better when they get transplanted to 5 gallon pots

Lot of leaves taking forever to trim

took all the branches of these 2 and used LST to move them out from the main stalk just like you were putting up an artificial Christmas tree. Shit these 2 girls are smelling sweet and dank and are stacking so large now. Can't wait to see how this one smokes.

These ladies are doing so great! Stretching up creating flowers along the way! I may need to trim the lower branches in time in order to not waste necessary energy being a solo but I’m feeling when I run Anesia autos in bigger pots in 6 weeks they’ll be no issues!

Week 6! Day 44: 2L (PH6.3) + half-dose 4-1.5-4 + 1ml/L CalMag Today. I learned that with half strength Bloom Feed and a dose of CalMag Pro, the PH of the solution ends up at exactly 6.3 - this means that most of my watering of last grow was too acidic because I often still added PH- Nonetheless, while checking up on the NLA I noticed her symptoms progressing and I did what any self respecting grower would do: remove the evidence. Coincidentally, I've removed most of the damaged leaves to make checking up on her easier and to more easily figure out whether she's improving or deteriorating. Additional thoughts: While inspecting the pictures again, I noticed that perhaps some of the damage might be a pointer towards the phosphorus toxicity theory, caused by the premature PK13-14 feed, and the additional feed of last week. That said, perhaps it's a convenience that the Pokon bloom feed does not contain that much phosphorus relatively - and since it's an organic nutrient it will not immediately raise phosphorus levels even higher. Hence, this week will mostly be focused on monitoring her symptoms, while the buds are steadily thickening up. Day 46: Moist, no water. Her symptoms are progressing, I noticed that some of the leaves have a tighter gradient from green at the stem, to yellow about half way the leaf. What this means? I have no idea, I'd like to think it's a response to the fresh supply of Nitrogen from the bloom feed. However, it's just as likely that this tighter gradient went unnoticed until now. She's got some pretty rough nute burn, so I think the best course of action is to stop feeding her entirely for now. I'm quite certain that, with the PK13-14 and the organic bloom feed, she'll have enough nutrients for the coming period. Buds are thickening up nicely though. Her terpenes are starting to develop and I can see more and more trichomes appearing. Day 48: 2L (6.3PH) Her symptoms progressed even further, I'm not worried though. I removed more leaves from the lower, shaded area.

Start of Week 8

new grow started by @polasekplants 😁 This time there are blue dream, girl scout cookies, forbidden fruit, and white kush seeds started in a peat moss soil with about 60% already pop. Mars hydro tsw2000 is set at 25% power and at 36in above the seedings.

Started flowering on Day 55

Día 73 (12/08) Riego 500 ml H2O pH 6,55 Todas las plantas muestras las preflores hembras! (excepto LemonPaya) Día 74 (13/08) Riego 250 ml H2O pH 6,55 . Están muy bien hidratadas Pequeños ajustes de LST Día 75 (14/08) Hoy día de lluvias torrenciales Riego 250 ml H2O pH 6,55 Día 76 (15/08) Detecto mosca blanca en varias plantas. Aplico Spruzit a 10 ml/l ahora que aún no estamos en floración Riego 500 ml H2O pH 6,55 Día 77 (16/08) Riego 500 ml H2O pH 6,55 La mosca blanca ha desaparecido completamente Día 78 (17/08) No riego. Mañana a primera hora aplico Top Dress y riego profundo Va a empezar la floración! Día 79 (18/08) Alimentemos el suelo con Top Dress! 💥 Aplicamos 4 g/L sustrato de Tasty Flowers TD by Lurpe Solutions. Total = 84 gramos / maceta Riego con 1 Litro H2O pH 6,5 con 25 ml/L de Humus de Lombriz Liquido Aplicación foliar Kelp hidrolizado de Lurpe Solutions a 0,25 ml/l 💦Nutrients by Lurpe Solutions - www.lurpenaturalsolutions.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Honestly these weeks are boring. I’m gonna jinx myself but I’m not having issues, problems, anything. My plants are thriving they’re growing at extraordinary rates and they’re perfectly healthy. I’m no master grower but I’m really happy with where things are. My temps, humidity, light and nutrients all seem to be on point and in the best place I can have them. I’m about 2 weeks out from flipping I think. Probobly less. The scrog is about 70% full and I’m shooting for 85% ish. I have no complaints or problems to whine about. Basically I’m ready to manage the largest grows in Colorado. Lmfao. Me and my two plants have this shit nailed down. But in all reality things are going great. They’re anti climatic and I’ve got some healthy, happy plants. I’m proud of the grow.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

HERE WE GOOOOOO!!! On time and looking great these Gotti O.G's was a 8 week indoor veg and now I'm kicking them outdoor for there flowering stage. Cali weather is great, daylight is at 13-14 ☀️. Thanx for all the encouragement through my veg stage I can't wait to show you guys the there harvest. 🍃🌿🌱🌴🌳🌲☘️️🍀

Despite her flaws due to my own fault she was giving me a real cheese smell but now the wedding cake comes through and sweetens it up. She smells amazing

As breeder recomm

Lunes 26 de Agosto 2024, segunda semana de vida de estas preciosas, siguen en giffy, próximamente trasplantar a macetita de cartón, iremos a Walmart a comprar sustratito para ese trasplante.

Eccoci qui... Tutto va per il meglio, questa settimana non ho applicato stress alla piccola, ma ho guardato l'evoluzione che ha avuto dando i nutrienti. Ora ha un odore davvero intenso ed uno strato di resina pauroso, vediamo come si riprende dalla defogliazione che ho fatto settimana scorsa. Seguiranno aggiornamenti, grazie a tutti per il supporto🔥🌲❤️

This plant is pure gold. It’s incredibly healthy and while my other plants are starting to get attacked by thrips a bit this plant seems unaffected 😎. I am up to 16 tops at this point so I have one more round of topping to get to the desired (32) amount of tops. After that it will just be maintenance and watching her in all her glory. I hope I can keep this lady wishing the limits of my greenhouse 😅. Humboldt nailed it with this genetic mix! Onward !!! 😎🍿🍻