Week 18 Day 120 (11/12/2020): I checked the girl’s trichomes and they are looking great. They are definitely getting more amber but still a small percentage. Also most of the pistils have browned now so I think she is about 2 weeks away from harvest now. So I will start the flush at the next watering! Day 121 (12/12/2020): The girl is looking good today but the leaves are going more an more yellow and crispy. Although I think during this time it is actually good news and I will be happy for her to take up all the nutrients left into the buds to make them extra tasty!🤤 Day 122 (13/12/2020)💧: Sooo this week is the start of the flush, just 1.5L of water. They are definitely ready for the flush and the buds are looking gorgeous! So gorgeous, in fact, that we cannot wait for the girls to be ready. So I cut some stems off to dry 🙊 We basically realised that they will not be ready and dry by Christmas (plus we would want to still cure them for min 2 weeks) and so I cut some of the smaller stems off to get some buds ready for Christmas celebrations 🎄 I only cut 1 stem off the girl and in the photo you can see how much smaller it is than the main buds. I’ve put the flowers into a box with holes in a room that has good airflow. I’ll also make sure to weigh and add it to the final dry weight. Day 123 (14/12/2020): Didn’t have time today to take pics of the girls but I’m sure they are fine 😉 Day 124 (15/12/2020): I swear the buds look fatter and fatter every single day that I check her. Looking great, smelling great, and will definitely taste great! Day 125 (16/12/2020)💧: Second flush of the week, great stuff. Just gave her 1.5L of water and ripped off some dry leaves. There are some stems at the bottom that are quite weak and cannot hold their weight. A lot of them have bent over and I don’t think they will get enough water to survive until harvest. At this point it is too late to cut them off but I have definitely learned to lollipop for the future! Day 126 (17/12/2020): What a great end to the week and just 1 more week to go! Buds are looking astonishing at this point. And I swear they are getting more and more red even though I thought they wouldn’t. The trichomes are developing beautifully as well, definitely harvest-ready in just a few days! And next week, I will be watering twice and probably harvest after the whole week is finished, so on Christmas day! What a great present 🎁

Han aguantado a la perfección los errores de un novato en interior cómo yo😌 tuvieron un ciclo vegetativo un tanto turbulento con estrés lumínico, estrés por frío, hídrico también ya que las llevabas demasiado cortas de agua y causa de esto un ataque de araña roja 🕸️ a pesar de todo han florecido a la perfección dando 95g en seco de un producto de muy buena calidad 🤩 Ha sido un placer compartir mi primera experiencia en interior con vosotros. Con ganas de empezar el siguiente y seguir aprendiendo y mejorando

Die GorillaZkittlez wurde an Tag66/45F abgeschnitten und ging in den einzelnen Trieben zum trocknen in einen Karton wo ein kleiner PC-Lüfter verbaut ist um die Luft in Bewegung zu halten. Ich musste leider wärend dem ernten wieder einiges wegschneiden. Das nasse Wetter hat etwas schimmel in die Blüte gebracht. Insgesamt sind dadurch bestimmt ⅓ - ¼ der Buds im Müll gelandet. War bei dem Wetter der letzten zwei Wochen auch zu erwarten. Um den Rest der Buds safe in die Gläser zu bekommen, gab es für die buds einen ordentlichen wet-trim um weiteren schimmel zu vermeiden. Nach 5 Tagen waren die buds ferig um ins Glas zu kommen. 20.5g kamen am Ende trocken raus. Bei ca 10g die schimmel hatten, ist das Ergebnis für die kurze Zeit und die erste outdoor-ernte halbwegs zufriedenstellend. Der Geschmack und die Wirkung trösten allerdings über den Verlust bei der Ernte hinweg. Das würzige dominiert den Geschmack. Allerdings kommen fruchtige untertöne im Abgang zum Vorschein. GorillaZkittlez kam wärend des Wachstums gut mit dem wechselhaften Wetter zurecht. Nur die Buds nicht🤣 Allerdings hatten die Ladys auch keinerlei Schutz vor Regen und waren dem Wetter komplett ausgeliefert. Der strain wird trotzdem wieder sein weg in den Garten finden💚

Another beautiful lady chopped and drying with that whole plant hang really looking forward to these but they are so Frosty and so dense thank you for these amazing genetic and this amazing contest you guys really know how to bring the world together thank you again 💚👑👊🏼😎💨🏼

Advertised 56 day flowering. Mad yellowing I think is root bound stress, over watering/under watering, magnesium deficiency... I have no idea. I top dressed with myco, azos, and a bunch of soil to give her some extra food for the last 3 weeks. I'm hoping it'll solve any issues. I'm going to put the airpot into a 7 gal cloth pot to try and relieve some root bound issues. I never intended for her to be this big in a 2 gallon... She was an auto after all that never flipped.

Day 72: 600W LED, 18 hours on 6 hours off the same with ventilation. Ferilization is the same except BlackBerry Kush and LSD-25 they are being flushed. Water intake also remains the same 200ml per day. Humidity approx 35 percent. Day 74: BlackBerry Kush and LSD-25 have to be harvested this week Friday so no more water. Colorado Cookies and Northern Light next week so started flushing them. Glueberry OG needs a bit more time.

Week 6 went well. The buds are putting out a second wave of pistils and are starting to fatten up. Jilly is starting to smell more and put out more trichs on sugar leaves. She smells very sweet and fruity so far.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

He elegido una red metálica. Es muy fácil de instalar y permite un control eficaz de las plantas. El resto es igual a cualquier cultivo hidropónico. Subo el nivel de nutrientes cada día.

wonderfull colours and smell, plant is looking fine, i see another 2/3 weeks from now, for a full development of the buds. heavy and frequent rain occurring, didn't have to water so much, hope it get better from here to the cut. i am gonna decrease on the nutrients, to make them disappear in 10-15 days and a have a full week on just pure water. the quality of the smell is incredible, very sugar-candy like, not so citric.

elongated stems made me worry, but they only needed little support at day 3 I started spraying them every 2.5h with filtered water

Checkout my Instagram @smallbudz to see the Small budget grow setup for indoor use, low watt, low heat, low noise, step by step. 27/12/2019 - Fed her 1.5l of 6.4PH water with 0,5ml of each: Grow, Bloom and Max, and 1ml of each: Heaven, Alga-mic and Vera, I use about 1/3 of the nutrient dosage on the chart, to achieve about 200/300PPM (500 scale). 01/01/2020 - Fed her 1.5l of 6.4PH water with 0,25ml of Grow, 0,75ml Bloom and 0.5ml Max, and 2ml of each: Heaven, Alga-mic and Vera, mesured about 280PPM (500 scale).

First plant already shows signs after 2 days.

Over all was an amazing grow, super sticky dense nugs with smell of berries, highly recommended for everyone!!

Die Buds zu trimmen war überhaupt keine Arbeit die wenigen Blätter zwischen den Blüten sind Harzig und entferne ich wenn sie trocken sind. Die Terpene sind zu ende hin extrem gassy geworden das es schon Chemisch Süß riecht ! Freue mich schon soooo aufs verkosten

Very satisfying. Waiting to more upload.

Amazing told this run 110 grams of bud and 80 grams of shake. An amazing pungent fruity citrus cream cheese aroma eminates out of my cure vault. The high has been described by my friends as a creative uplifting high followed by intense couch lock. The grow itself was a breeze. The only real issues i had in the hydro grow was that i needed to to add cal mag because it was growing so fast and i was using distilled water. I basically did not train my plants other than defoliating to make space for air flow and light penetration. I would recommend this for any first time hydro grower that has a decent amount of space.