Week 21 count down to the end of grow

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Week 25 overall, corresponding to week 3 of flowering for the Pineapple Upside Down. Flowering has now clearly declared itself. Bud sites are visible everywhere, with consistent pom-pom formation across the canopy. Despite being at a relatively early stage of bloom, terpene expression is already strong. Upon opening the tent, a distinct pineapple aroma is noticeable, which is very encouraging for such an early phase. Vegetatively, the plant will not recover its original leaf coloration. This is accepted at this point. However, flower development appears to be on track, and the plant is allocating energy toward reproductive growth rather than foliage recovery. A light defoliation was performed, along with the removal of lower, non-productive flower sites. This cleanup aims to avoid wasting energy on underdeveloped buds and to focus resources on the upper canopy. A fresh flowering nutrient solution was prepared. PK has been introduced lightly, in accordance with the Athena feeding schedule for week 3 of flowering. Electrical conductivity is set around 2.1 EC, with a target pH close to 6.0 to ensure stable nutrient uptake. Light intensity will be increased progressively, with PPFD moving toward approximately 600 to support proper bud development and flower density. Although the foliage remains visually poor, flower formation, structure, and aroma remain the key indicators at this stage. Given the short flowering time of this cultivar (around 45 days), timing is critical, and the next weeks will be decisive. The focus going forward will be on stability, controlled feeding, and optimized light management to extract the best possible outcome from this cycle.

Día 57 (10/03) Riego con 400 ml H2O EC 0,45 Día 58 (11/03) Los cogollos no paran de engordar y ponerse duros como rocas 😍💥 Riego con 500 ml H2O EC 0,45 Día 59 (12/03) Se muestra llena de tricomas! Espectacular! Riego con 500 ml H2O EC 0,45 Día 60 (13/03) Riego con 500 ml H2O EC 0,45 Inicio un experimento a ver cuantos días aguantan sin regar habiendo regado dos días seguidos con 500 ml, ya que voy a estar 3 días fuera la semana próxima.... Día 61 (14/03) No riego Día 62 (15/03) A las 48 horas las macetas empiezan a estar ligeras, aunque no secas, pero NO van a aguantar 3 días sin riego Riego con 350 ml H2O EC 0,45 Día 63 (16/03) Aplicación de 0,5 Litros de PK Booster Compost Tea de nuestros increíbles amigos de BIOTABS 🚀 FastBuds 15% DISCOUNT code "NONICK" 2fast4buds.com @fastbuds.official 💦 BioTabs 15% DISCOUNT code "GDBT420" biotabs.nl/en/shop/ @biotabs_official 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE @promixmitch @promixgrowers_unfiltered 💡2 x Mars Hydro FC1500 EVO Led Grow Light (2024 NEW FC 1500-EVO Samsung LM301H 150W LED) - https://marshydro.eu/products/fc1500-evo-led-grow-lights/ - https://www.amazon.de/dp/B0CSSGN5D8?ref=myi_title_dp

Day 84 - Truckin steady! 2.5 gallons dosage of the above nutes every 4 days! This weeks food has the addition of dry koolbloom (3.1 grams / 2.5 gallons). Just plucking the occasional leaf that looks at me funny, and watering when dry! (every 4 days)

Great so far

🌈 Mental Rainbow – Week 5 Update 🌱 This lady is taking off! 🚀 In just one week, she packed on some serious size and is showing her full potential. The leaves are a deep healthy green, and the structure is getting stronger day by day 💪🌿 Really excited to see how she’s going to stretch in the coming weeks – it already feels like she’s preparing for a big show ✨. Follow along if you want to see this rainbow shine 🌈🔥. 💚 Stay tuned – the best part is yet to come! --- 🌈 Mental Rainbow – Woche 5 Update 🌱 Die Kleine legt richtig los! 🚀 Innerhalb einer Woche ist sie ordentlich gewachsen und zeigt, was in ihr steckt. Die Blätter sind kräftig grün und die Struktur wird jeden Tag stabiler 💪🌿 Ich bin mega gespannt, wie sehr sie in den nächsten Wochen noch stretchen wird – man merkt schon, dass sie sich auf ihren großen Auftritt vorbereitet ✨. Bleibt dran, wenn ihr sehen wollt, wie dieses 🌈 richtig zum Strahlen kommt 🔥. 💚 Das Beste kommt erst noch!

Very explosive growth this week! This girl has the most enormous lower fan leaves! Things progressing very well.

Flushing one plant. The other plant i might start flushing next week. Cleaned up the late flowered plant. Defoliated a few leaves.

Little airy buds,not as fast flowering but really good colors,she needed one or two weeks for sure but i didn’t have time,I will grow it again for sure.

Girls start getting bigger :) One of them it looks like will be finish soon 🤨 this week i will get microscope then i will check on her what trichomes are 🧐✌️

La pianta cresce a pieni ritmi, sono sempre più sorpreso di quanto sia resistente questa varietà. Ho scalato un pochino n perché si sta avvicinando alla vera e propria fioritura, sto cercando di mantenerla il più basso possibile. Ho defogliato un pochino sono indeciso invece se levare tutte le future cime pop corn e lasciare solo gli apici voi che dite? Comunque grazie a tutti per aver seguito questa settimana ✌️💯💣

Day 15 Mon PH 5.8 EC 0.6 PPM 300 DLI 19h PPFD Water 20 Day 16 Tue PH 5.8 EC 0.6 PPM 300 DLI 19h PPFD Water 20 Day 17 PH 5.8 - EC 0.6 - PPM 300 - DLI 19h PPFD Water 20 Day 18 Thu Add A&B 2.5 mll PH 5.9 - 5.7 EC 0.6 - 0.8 PPM 300 - 400 DLI 19h PPFD Water 20 Day 19 Fri PH 5.7 EC 0.8 PPM 400 DLI 19h PPFD Water 20 Day 20 Sat Add Ph Up PH 5.4 - 5.8 EC 0.8 PPM 410 - 420 DLI 19h PPFD Water 22 c Day 21 Sun PH 5.9 EC 0.8 PPM 420 DLI 19h PPFD Water 21c

Well this week has gone a bit pear shaped. I think it is a combination of light stress and the new soil I'm using. Also the soil had fungus gnats. I won't use it again but it was an experiment and I've learnt that soil isn't my substrate of choice! Back to my super coco mix! 😁 I'm going to see if I can pull it through. I've got plants through worse! Wish me luck. I watered twice this week with 1ltr of dechlorinated water PH'd to 6-6.5, containing 1ml of Ecothrive Trace and 1/4 TSP Ecothrive Biosys. I will say that the soil is very difficult for me to judge the moisture level to water accordingly. Have I mentioned it don't like soil? 😒💚😂🍃✌️ Thanks for checking out my diary this week, I'm sorry its not a better update, but wish me luck! I'm going to need it here! 😂💚✌️

This was the last week for the Cookies Gelato. They've turned purple. Weather is not that great anymore, and I want to avoid more budrot, so I had to chop them down 😛

Day 41, start of week 7 tomorrow!

Girls jus doing their thing living their best

lil be hide put in the work thought id share with you guys the 30 min ride 20 min walk boy im glad the hard works be hide me time to sit back and injoy the sunshine sound of water i love this beautiful