Keeping moisture around bluemat 90-120. Little bit defloration and LST. She is making the transition to

If anyone has any advice on how to improve this grow please comment below. thanks for checking out my diary! End of week 3/Day 27: ive been flushing alot before giving nutrients to the plant and it seems to be working, plant health is slowly improving again! Started LST a few nights ago and my plant has lost its christmas tree shape :'D the canopy is more flat and more light is getting to all growth on the plant so im happy! I started giving some rhizotonic near the stem to help with the plant being stressed. it definitely helped make big roots too! So excited to try some buds! another month or so yet :) Day 28 - 20/10/19 LST is amazing! i have almost all the growth getting a decent amount of light! im going to enjoy shaping the plant over the next week or so! Day 31 - 23/10/19 Still doing a light LST, most of the canopy is getting enough light now and the main stem is still growing more nodes. After getting some advice from a grower with the same model of light as me i now know i can hang my light a lot closer to the plants. im going to get rope rachets hooked up in the next week so i can adjust hang height. I also got an indoor chilli starter kit and planted jalapeño, cayenne and habanero seeds in some nice small terra cotta pots :) and i posted a few pictures to show the setup :) just cant wait until the whole tent looks more like a garden hahah :) 12 hours later - Added a video to show progress Day 32: 24/10/19 woke up this morning and the quick one grew upwards out of the LST :) the top has developed and looks more like an industrial weed plant now which is really exciting! ive been following a water schedule and accidentally gave a few more hours of rest to the plants last night and they have stopped drooping more or less. Re-lst'd the plant so all growth is receiving light and measured the space between the plant and grow light. its about 60cm/24Inches away which will do fine. Quick one is 18cm with LST, if i measure the stem itself its about 21-22cm tall - about 9 inches. Other than that the chillis will take up to 2 weeks to germinate, ive been keeping the moisture up around them with a mix of Rhizotonic/Water (1ml/1L) and also sprayed some on the soil as ive found it actually helps seeds germinate quicker with more strength or life in them. Will update soon for week 5 and considering the smoke is so bad in my town you get an urge not to smoke it i am so excited to be near the finish line :) roll on medicine!

Time to take off a few fan leafs. Most plants are 5-7 weeks old. (Start of week 7). 2TD just popping their heads up. 2 - LZ, 2 - CJ and 1 D are 2 weeks old. Hate to spread it all but but just made a big move into a new home... the older plants were stressed to much in the first few weeks and it shows in their size. I have higher hopes for the new plants and they are looking on point for sure. Love organic weed!!!

ok so everything has gone fantastic , no nutrient burns at all , and have been slowly creeping up the base nutrients , i have stopped the cal mag and now adding house and gardens bud XL at full strength these girls are now in full swing of flower and i bought a new ph stick this week , i have found when PHing the feed bucket that its best too have air stones in the bucket , add all nuts and then ph but do not use the feed that day as if you check the next day the ph is off again so i left it 24 hours after i add more ph down , and it seems too make the measurements alot easier and stable , i have bent down the main stems " without damaging them onto my netting too A keep the canopy all the same high so my lighting is covering all plants at the same distance and also it allowed the side stems too reach up too , i also was sponsored another new led light by kingbo this week so have added another 600watt led too the grow , so now have two 600's and one 900watt , and temps are still fine sitting at 24c lights on and 19-20 off , so am pleased with that , this lady is nice and compact and tight lots of leaves that I had too remove too open up her bud sites , stretch was also minimal ,

These ladies here have been fighting me all the way lol but everything still seems to be coming out good. They are stacking nice and buds are rock solid already. Just gonna keep doing what I can to keep these 2 girls happy lol.

Well hello! Thanks for checking out this new week for the mac 1's from Zamnesia 3 of the 4 are growing quite uniform, but 1 is a bit slower as you can see on the pictures. I hope she will soon catch up with the others, but we will see how it goes. Stay tuned if you are also interested!

Something is fckng with me heavy,I'm using tap/RO water 50/50. and still my cal mag issues are getting worse..I'm hoping that I will be able to finish this grow without big loss in potency or yield .

It seems like she needed to be closer to the light so I moved her a closer. She is growing very tall and fast. I water her once every two days or when I see the soil is dry. :)

2nd time around Miss Candy Auto is doing very good. She is putting on weight in her buds and Getting very frosty. This Fall weather is much better than the dog days of summer. I stole a bud off of her so I could use my max-see to get a few close up pictures. She isn't just ready for a harvest yet but she is close! Stay tuned and Be Happy! Comments and Likes appreciated.

She only grew 2cm this week so that means she is is full flower mode. Look how much the trichomes have increased this week! I've never grown a CBD strain before, is this what they do? 😃 Minimal input from me this week. Just watering.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Can't wait to smoke.

Die Blätter haben durch die Blattläuse gut gelitten. Jedoch wachsen die Blüten gut weiter und sehen auch sehr schön aus und die neuen kleinen Blätter haben wieder ein sattes Grün. Die Blattläuse waren weg, danach wieder da weil sie wohl doch nicht ganz weg waren. Florfliegenlarven sind morgen da. Ab jetzt noch ca. 5 Wochen zur Ernte.

Week 4 flower - Day 27 of flowering - Day 83 of life Black gum is getting fat and frosty. The 2 main colas-buds are looking good Middle flower now so hoping they will progress even more. Lets go

Bueno, empezamos por el final familia. Gorilla girl, me parece una cepa BRU-TAL , ya partiendo de esa base, mi error, creo que e cometido , es poner estos ejemplares en macetas de 5L, que tonto... mínimo recomendado 7L y a ser posible 11L Otro error fue no dejarlas una semana más. pero, escasez. (intentaremos curarla un par de semanas). Cepa con flores muy compactas, hay bastante separación entre nudos pero compensa las flores de verdad. Sabor muy dulzón a mi me recuerda a melón , es un pepino recomendado para amantes de la hierba que llevan tiempo en esto, un colocón muy fuerte, ahora mismo estoy bajo los efectos de esta vaina, aseguro que con cada frase, leo varias veces y detenidamente por que válgame.

It is getting real bushy in there and i have some thirsty plants. Tent is getting pretty packed and the stretch is on. I might have to water almost everyday, I suspect them to be on the edge of rootbound. Should have went with 14 liter pots or might even 18. Smell is amazing and they are starting to get sticky when i pick fan leaves. Defoliation is coming at the end of next week tho. But it is really bushy i have been picking tons of leaves so far. Tempted to strip them sooner than 21 days into flower but we will see.

This week im going to let it grow and fixing the LST.