SPONSORED DIARY===SPONSORED DIARY===SPONSORED DIARY Seeds finally popped up and out of the medium, giving them 24hr lighting till week 3 then will reduce it down to 18/6. Added in a few pics this week of the tent and light. nothing much else to report. SPONSORED DIARY===SPONSORED DIARY===SPONSORED DIARY Big shout out to @SpiderFarmer for supplying the 2x2x5 Grow tent and The SF1000 100w LED light. I will be uploading all relevant photos of the products in the next week when they arrive, maybe do a little unboxing video. Who knows. A 2nd big shout out to Dr.Seeds for supplying the 3x5pac of seeds I will only be featuring two of the three strains in two separate diaries. You the users will get to decide what ones I grow. I have setup a Grow Question on each diary to vote for your strain of choice for that diary. https://growdiaries.com/grow-questions/47600-drseeds-1-vote-what-i-grow "Diary #1" https://growdiaries.com/grow-questions/47600-drseeds-1-vote-what-i-grow "Diary #2" Cast your vote for each diary. Thought I would add in the relevant links if you wanted to read up on any of the products that will be officially featured in this grow. https://www.spider-farmer.com/ https://growdiaries.com/grow-lights/spider-farmer (+) https://www.spider-farmer.com/products/sf-1000-led-grow-light/ https://growdiaries.com/grow-tents/spider-farmer (+) https://www.spider-farmer.com/products/sf-2-x2-indoor-grow-tent-hydroponic-home-plant-reflective-aluminum-oxford-cloth/ https://drseeds.net/ https://growdiaries.com/seedbank/dr-seeds (+) https://drseeds.net/product/cherry-pie-autoflowering-cannabis-seeds/ (+) [No relevant GD affiliated link] https://drseeds.net/product/granddaddy-purple-autoflowering-cannabis-seeds/ (+) https://growdiaries.com/seedbank/dr-seeds/granddaddy-purple [Photoperiod not Autoflowering] https://drseeds.net/product/60-day-wonder-autoflowering-cannabis-seeds/ (+) https://growdiaries.com/seedbank/dr-seeds/60-day-wonder-autoflowering

So after an hour of trying finally uploaded a video

Noch immer nicht fertig ... ;-)

Well, there’s no ghost the VIVO sun pumps that come with the kits they just suck that’s OK. I replaced them with the upgraded version. I refilled the reservoirs

Fattys are growing and seem to be thirstier.Used foliar spray to help the leaves and also started tucking. When the plant was topped pistils were seen for first time.

Hasn't been much. Feeding and letting her grow Taken allot of smaller buds of that will not develop. The canopy is pretty tight but i have the 4 fans going 2 high & 2 low should be giving plants enough breeze. Starting to bulk a bit. Going to be starting the flush this week and adding allot of sugar.

She seems to thrive in the new soil, I'm checking her regularly because of the darker ridged leaves, could I have overdone the bloodmeal content? It should not have overfeeding effects with organic super soil. But we'll see. The FIM seems to have taken hold with her and today I started training, although it is more main-lining. For this, I just used some microwaved bamboo from the garden. Now we go towards flowering and I'm getting more excited after the first few weeks were a bit sad. Some of you might have seen my super soil recipe in my other grow, the Orange Hill Special, if you didn't, check it out here in the images! I designed it out of frustration that products like Fox Farms etc are not readily available in Europe. Those who saw my Gelato Monstercop grow, know that soil was pretty good, that soil became the base for this recipe, amended with some organic precursors to make it less dependent on feeding and after amending. I would recommend amending some before flowering starts without the bloodmeal. Hope you can use it!! Thank you very much for checking me out again this week! Hapy growing and big hug BioBuds and Sunshine

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Ehi ragazzi come va? Tutto bene spero, la ragazza sta esplodendo di vigore!! Nonostante il poco spazio si sta facendo spazio tra le F1 e sta creando le sue cime come fosse la regina della grow! FastBuds mi stupisce ogni volta! Le loro genetiche sono spettacolari, ma il merito è anche della creazione del terriccio ideale della bio tabs che la sta facendo crescere come un baobab ahahah ! Si vedono le prime cime e le foglie si sono sviluppate davvero grandi e piene di vigore si vede che la ragazza sta bene e vuole dare il meglio di sé nella sua breve vita , spero esca una ganja davvero speciale da questa Tropicana cookies ,un sacco di cime resinose e piene di thc! Ringrazio Mars hydro perché il merito della crescita spettacolare di queste ragazze e della sua luce ,che a me sembra perfetta per intensità e distribuzione.. grazie a tutti voi che mi seguite ! Per questa settimana penso sia tutto alla prossima! buon 420 a tutti! 🐱😼💪🧙‍♂️🌱🌿🌲💗

All went well this week, apart from my phmeter breaking... I guess more two weeks of waiting. The colas are well developed, but the lower flowers ain't. But both are full of tricomes.

Not much to say about this real first grow week’s start. Everything looks ok, steady growth at 40% light. I had to restart the control app a few times, therefore the broken graphs. At night times, the control software tries to attain optimum humidity rather than optimum VPD. That’s why VPD is dropping when the lights are out. CO2 is back to baseline – really wonder what the reliable values are … I found I have to silence the blowers when a timelapse photo is being taken. The shaking you see at the beginning of the grow video should not appear, or to a much less degree, in the future. Day 13 (July 30th) shows good, steady growth. Leaves are a bit spotty, although less then the processed images show. Something to be worried about? I would not currently … All are developing their 3rd nodes now. The controlling Raspberry Pi crashed last night when I tried to access it remotely … ;( – looks like the Connect software is in need of an update. The leaf temperature sensor is now really aimed at lady-to-be nr. 1’s leaves. Nothing to change that I see. I won’t cut the top yet, just watered the outer circle of the big pots slightly to encourage root growth. Day 14: Nice growth. First plant is now at 11 cm, and at 12 cm on day 15. Nothing special to report so far, except for I had to move the internal camera a bit upwards to keep little lady #1 in focus. Day 16 and last day of this week (Aug 2nd): The young ladies are now at about 14 cm tall. Growth looks very compact though. I think the two inside girls will lose their heads next week; I’d still prefer to have one or two more nodes available. Pictures of this day are taken at the end of the plants’ day. Notice their leaves are pointing upwards, which should be a sign that they want more light. I turned the lights up by another 20% for a total of 60 W now. Weather was pretty hot the last days so it wasn’t possible to stay in optimum VPD range but as long as growth is this satisfying I don’t mind. I will water them slightly at their next day start. Outside plant’s soil humidity might still be at around 58% officially, but drying starts at around 40% with this measurement and the sensors are placed quite closely to the stems so outer soil is dryer. I am preparing the plant water with some additions and a frequency imprint suing some Solfeggio and Schumann frequencies.

Comienza la floración ya podemos ver algunos brotes

Fin'ora tutto bene a un mese di fioritura ho cambiato l'acqua, c'è un problema l'acqua è a 25/26° ogni tanto ci metto delle bottiglie congelate e aumento il perossido d'idrogeno

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I have to harvest next week im on a scedule :/ she looks decent The run off was 1400ppm so i flushed and feed back with 650ppm

Hello, We started week 10, 2 days ago. Bubble Kush. Some deficit of calmag, and phosphorus, they are very hungry. Gold Merry have 9 weeks. I feeding every 3/4 days 1,5 l. On the photo the feeding schedule.

Alright Week 1 of flower.. whew I'm here and everyone looks Healthy and Happy and On Track... I think... Let me know Y'all it's kinda quite.

Well well well ! I'm planning to cut eveyone in about 10 days. Just watering and slowsly starting to remove fan leaves Pretty happy about the conditions in my room, i have around 45-55% RH and maximum 24 degres. The drying will occur in this growbox, so i think i'll can have a nice and slow dry See ya next week for the last 10 days 😍