3/29 - I'm not sure she needs another topping. I'm just going to spread out the current tall shoots and let her rip upwards! Also swapped the light for the final veg lamp. 3/30 - training continues and she is taking up more water every day! 3/31 - feeding and defoliation of the larger and lower fan leaves 2L distilled water 5.9pH 65*F 780ppm Grow 3.02g Kelp 0.14g Cal/Mag 0.56g Amino Acids 0.48g Enzymes 0.15g Microbes Grow Stage 0.53g Real Grows Recharge 0.1g 4/1 - Growth is significant since defoliation, I think it was well timed, she grew an inch overnight 4/2 - Great Friday! Another day of training and she is starting to look amazing! 4/3 - feeding day, took off some large fan leaves and trained just a bit more. I think this week she will be allowed to start growing vertically 2L distilled water 6.0pH 1040ppm 71*F Grow 3g Kelp 0.17g Cal/Mag 0.56g Amino Acids 0.5g Enzymes 0.14g Terpinator 5ml Real Grows Recharge 1.26g Great White 0.15g

🌿 Grow Update: Day 2, Week 8 of Flower 🌸 Hey fellow growers, We’ve hit Day 2 of Week 8 in the flower stage, and wow, she’s smelling delicious! The buds have definitely grown since the last update, but nothing too dramatic. Still, her appearance, aroma, and stickiness are on point. Not too shabby for my first run! There’s a fair amount of larf below that I’ll be tossing, but the top buds? Holy smokes, they’re looking fantastic. I'm aiming for at least 3 ounces, and if I hit that or more, I'll be over the moon! I'll be starting my 2-week flush very soon to ensure the best quality for the final product. Wish me luck as we head into these final weeks. Let’s finish strong! 🌿💪 #GrowLife #Week8 #FirstGrow #FinalStretch #FlushingTime Note : Good job i installed a scrog net as checkout my buds in the video posted they can barely hold themselves up if i never added a scrog id of been screwed would of either had to constantly check and tie them up or worst case scenario id of gone in the room and came across snapped branches bent over!!!! SCROG IS OP for huge plants

She is officially 3 weeks old yay ! And female double yay! And I believe she is growing strong. Started showing what I believe are pre flowers ... ? No smell yet and is she small for her age ?? Not sure since it is an Autoflower. Increased Nutes and added a little bloodworm meal to top soil . Grow baby grow , super excited 😁

Just on day 22 now the video taken this morning had few issues very strong through week one, then week 2 I believe nitrogen toxicity as iv done lots and lots of research.... started watering twice in between each feed.. showing pistils now.. plants were tied down day 14.. I am happy with progress overall any tips welcome Pic of individual plant seems to be behind and leaf curling going to give water today and hopefully that will sort it out Day 23 - decided to do some defoliation as the growth since yesterday is very noticeable and starting to see signs of budlets at apex.. going to feed them tomorrow 8th strength grow, 8th strength bloom then by day 30 ish just quarter strength bloom is the plan then continue to up dose until half strength bloom at which point I’ll also add some boost.. any pointers appreciated 👌

Creció mucho (especialmente para tener apenas 150 watts). No creció en altura, lo cual es bueno. El corte apical quedó bien.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

The girls develop properly, even "Freako" promises to be interesting despite the hard experiences 💪

La 3 semana de crecimiento las niñas están en su máxima potencia comprobamos que no tienen falta fe ningún nutriente están super verdes con unas hojas muy grandes

Day 1 - TC600 is 36 inches from seedling. Veg 100% Flower 100% 17 hours on 7 off. PAR/PPFD 170 DLI 10.05 Day 2 - Mylar Emergency Blanket DIYed into 2 by 3 foot tent. PAR/PPFD 240 (41% increase) DLI 14.5 (44% increase) Nutrients: Advanced Nutrients 2 mL of each Micro Grow Bloom Base Nutrients and 2 mL BotaniCare CalMag. 3 Gallon bucket filled with 2 gallons of RO water. No more notes for rest of the week. The TC600 is 260w from the wall. Not 600w.

GMO (Garlic mushroom onion) was slow to flower but came on strong weeks 8 and 9 to produce some dense heavy sticky buds. The dry weight of the plant material is unsure, but I’ll report the weight of various bubble microns when dry. Pictures of the dry bubble and maybe a melt shot impending! To dry, I decided to microplane into pizza boxes lined with parchment. It’s been fun growing along with everyone on grow diaries. Awesome

30.04.2024 Continue into week 9 Clean nutrient tank for the last time...This is last week of flowering for almost all girls. Electrical Conductivity (EC): 0.8 for this week.. Purple Lemonade: First pheno is insane! Shape, size ,color, bud hardness, appearance, frostiness' all attributes to become photo model! Beautiful and high odor in the room! Lighting Parameters: PAR Map: Maximum PPFD is 800, minimum PPFD is 550. VPD: Ranges from -0.1-1.1 Daytime Temperature (DT): 23-24°C. Nighttime Temperature (NT): 19-20°C. Leaf Temperature: 21-22°C. Light Distance: 45-55 cm from the plants. CO2 Levels: 700+ ppm. HLG Scorpion Diablo: Set at 60%, positioned at a 45-55 cm distance. Girls drink less water, so for this week and till the end of flowering new misting timing's Daytime: ON for 50 seconds, followed by 30 minutes OFF. Nighttime: OFF for 50 seconds, followed by 45 minutes OFF. Nutrient Tank (NT): pH: Maintained between 5.80 and 6.00. Stable PH, small drifting up and downs EC: 0.87 Temperature: 19-21°C

Vamos familia, actualizamos la segunda semana de vida de estas Panty Punch de Seedstockers, salieron 3 una se resiste a tirar adelante, veremos cómo progresan. Empezamos añadiendo varios productos de la gama de Agrobeta. Temperatura y humedad dentro de los rangos correctos. Muy pronto realizaré el trasplante correspondiente a 7 litros. Vamos viendo cómo avanzan estas próximas semanas. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

Hey guys! This week my pheno #1 was harvested after 12 days of flush. I forgot to mention that I've been flushing her before the others because she matured her trichomes earlier. She also started to flower a little more than a week before so I was expecting it. :) It was a good run for her I think, ended up with some dense buds that are drying in my dyi dry box, because I still have two other plants running in the tent. My house smells good 👍 I thought about doing the flush in the other two like I said last week, but watching their trichomes development I gave them one more week of feeding, because I think they still have some bulking to do. About the harvest of #1: I've ended up with 334g wet weight, and I will share all the photos and data in the harvest week with all together. Some of the buds are so frosty and they have like small resin balls that dripped from some parts! I've never seen this before in any grow of mine, but I've seen this at another diary here from the same strain. What a pleasant surprise! Have you all a great week, Felix ✌️

Hello my friends 👨‍🌾👩‍🌾, Let's go for a new run! First of all a big thank to Mars Hydro and @Coco_Pan for sponsoring the TS1000 ❤️❤️, as well as Sweet seeds for donating the seeds ❤️❤️ in a contest. I was able to get a glimpse of the TS1000 for a good part of the flowering of my auto mimosa, I'm really not disappointed with the result, this time we're going to start from the seed. First Sweet seeds photo, first seeding with the TS1000. The seeds germinated very quickly, opened in less than 24h👍👍, I put the seeds in plugs when the root measured 1/2 cm (48h), the same day 2 had already come out. Since it's my first seeding with the TS1000, I put it on the minimum, and created a little shade, 2 have a lot of stretch 😅🤦‍♂️, So, I put them in direct light. #3 came out of the ground after 5 days. I wrapped the roots around the plug then put the plugs in 2l pots, Plagron Light mix + rqs pellets. Today all 3 are in pots, About #4, bad news, I probably broke the germ during the translation, it is no longer connected to the seed 😪. I moisten the soil twice a day with my water + rhizo spray, maintain a temperature of 26°C. Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro TS 1000 https://www.mars-hydro.com/ts-1000-led-grow-light Gorilla Girl F1 fast version https://sweetseeds.es/en/photoperiod-dependent-seeds/3065-gorilla-girl-f1-fast-version.html

ainda tentando corrigir o erro do PH, lavando o solo com aguá PH 6.5, nao sei se vou conseguir reverter, o erro foi muito tardio, numa fase crucial, mas seguindo..

She looks super beautiful and healthy in her first week in this crazy world, she's a strong girl, she's developing not only correctly but very fast too under my HortiONE LED lighting. I'm using 2 panels of 190w each. Hope to train this lady and make such a beautiful bush using training techniques, we have a beautiful journey coming up guys! I'm growing my ladies reusing my soil and it's completely amended and full of microorganisms and life again using Organic living soil FLO By florians living organics, contains everything the plants needs at every single phase. So let's see what we can do this time! Let's get to work 💎💚🌱👨‍🌾

End of week 10 looks like 3 of the ladies will start getting flush next week the others look like they will go until week 13 or 14 definitely wasn't my plans I was hoping around week 11 everything would be done. But even tho they are all the same strain and dropped the same day they finishing at different times wired but ok. I'M READY TO CUT but I have to let them run they course Ps: the tricone production and smell is amazing on these plants once I pull them out of the net I will get sum awesome pics for your growers