All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Hello Diary. Finished and the third week of flowering, Purple Punch has reached a height of 67 cm and I think it has reached its maximum. 👆 All three plants are now the same height which is great considering the position of the light. At the end of the week I did a defoliation, I cleaned the lower leaves and branches on both plants, but most of all I had to fix their roommate Watermelon whose leaves took on a yellowish color with brown spots. Although the treatment was the same for all three plants, these symptoms did not appear on Purple Punch. If the cause is over feeding, then the conclusion is that Purple Punch is a much more resistant plant. 💪 The flowers develop nicely, are full of already visible trichomes and begin to take on an increasingly intense scent. After all, everything can be seen in detail in the photos. At this stage of flowering I started doing photos on a black background, the photos are much nicer. All in all, I am pleased with how Purple Punch is progressing, both plants look healthy and satisfied. I added nutrients when watering to finally see which dose is optimal for them, but given Watermelon, it’s hard to judge when each strain reacts differently. For now, Purple Punch is not complaining. 👌 Here is a brief overview of the week. 04/03/2021 - Day 37. Watering. I regulated p.H. at 6.3 with Plagron’s Lemon Kick, and added one Easy Bloom Booster tablets to a total of 7 gallons of water. With that, I watered all three plants evenly. Temp / Humidity on the farm - 25.3 degrees and 41% humidity. 08/03/2021 - Day 41. Watering. p.H. I regulated to 6.2, I added 1ml / lit CalMg and BioBizz as scheduled for the third week of flowering. Temp / Humidity on the farm - 25 degrees and 39% humidity. 09/03/2021 - Day 42. Defoliation and photography. I photographed the plants in the Box, to see the current condition and after that I did a defoliation. For the end, I took the final photos of the plant for the end of the 6th week. Temp / Humidity on the farm - 25.2 degrees and 38% humidity. That’s it for this week, see you soon with a new report. 🙏

Sie macht ihr Ding 🙂✌️🍀

questa frostbanger auto dopo uno stress iniziali si è ripresa super bene.......

Tag 116: Ab heute beginnt die Spülzeit, nur noch ungedüngtes Wasser. Und in 2 Wochen ist dann schon ernte, bin schon sehr gespannt auf das Endergebnis. Sie riecht auf jedenfall schon ganz hervorragend 😍

привет ребята,все идет хорошо , это от производителя и я так и не понял он цветет 6-7 недель или всего нужно Цветение 42 - 49 дня ??? Генетика Hulkberry x Diesel Auto Харвесты 500 г/м² Цветение 42 - 49 дня 42 - 49 дня вот такая у нас температура , нет денег нет условии,но не проблема стараюсь как могу )))))) ??? я нечего не трогаю,хочу увидеть полный потенциал )))))))))))))

Had a great week, fed her some nutes and noticed some pistils. She's flowering!

Tag 71, die 11. Woche hat begonnen. Die blüten wachsen weiterhin und nehmen an Volumen zu. Ich werde nur noch einmal mit halben Düngeschema gießen und ab dann ohne Dünger weiter gießen. Vorraussichtliche Ernte Tag 80-85. Tag 76, Spülung beginnt. (gießen ohne Dünger)

She's in flush, bulking up and smelling citrusy

Quite the nice Bush

#1 hatte 1 Woche länger gestrecht und wird auch generell 1 Woche länger brauchen als #2. Schauen aber beide gut aus! Ich bin sehr zufrieden, wirklich ein toller Strain #1 hat ein bisschen erdigere terps aber mit viel frischer Zitrone.. erinnert mich an die guava von Fastbuds #2 hat sehr süße Zitronenterps welche schon fast den Aktivkohlefilter an seine Leistungsgrenze bringen

Fin de croissance, début du cycle 12/12

31naposak ma 4nap telt el supercropping óta 2napja háló alatt vannak

Lunes 19 Agosto 2024 Semana 1 oficialmente como plantulas en jifys, dejaremos en jiffys hasta notar raíz abundante, jiffys hidratados con Agua con Enraizador Radix 10000. Veremos la evolución todos los Lunes.

Day 49- This week I think we cut out the nutes and let them finish, atleast for the mbrxgp. The Pablo’s revenge looks to be about a week or so behind so we’ll go until they’re finished. Smells on the mbrxgp is heavy citrus, lemon almost, slight gas. Pablo’s are very earthy, I’m expecting a hashy, dank finish for them.

~ FastBuds PAPAYA SHERBET Photoperiod~ Well here we go on another journey together through 'Canna-land' and this one's kinda special... forget that, it's VERY special because this is FastBuds newest surprise for the community, a FEMINIZED PHOTOPERIOD STRAIN!🤯 I was fortunate enough to score these seeds, of which only 1000 were available and can't wait to see what this strain can do! This Papaya Sherbet, according to FastBuds, is a hybrid with a 9-10 weeks flowering period. For a more detailed and accurate description of this strain the following from FastBuds says it best: "Combining the massive stature of Papaya (Oni Selection) with the strength and resilience of one of our best keeper cuts (Sunset Sherbet), this strain develops into a big, expansive bush adorned with numerous bud sites that later transform into a generous harvest of medium-sized buds. Papaya Sherbet flowers deliver a signature flavor of premium cannabis with subtle citrus undertones that emerge upon inhaling. During growth, her aroma makes for a sweet yet pleasantly bitter fragrance, giving you an idea of what the smoke will taste like. Notably stress-resistant, Papaya Sherbet is a great choice for growers working in challenging environments. She is very forgiving and rebounds quickly from any adversity, allowing growers the freedom to experiment with confidence that she will take everything like a champion she is. This strain embodies resilience, flavor, and abundant yields in every grow cycle." Sounds like an epic strain and I personally cannot wait for this lady to strut her stuff!😍 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the Papaya Sherbet photoperiod indoors as our weather is still a bit too chilly to put a newly sprouted seedling outside (nighttime temp's dipping regularly into the 40's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures are in the mid 50's℉, which shouldn't be long. After which, she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes from BioTabs and slow release dry amendments from Gaia Green. Once she's established herself outside she'll be given periodic top dressings of Gaia Green 4-4-4 and 2-8-4 along with worm castings and Compost Tea's. Her grow area is approx. 5'x5' and I have posts and a trellis net set up already for when she gets bigger to aid in training her. Let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 5/16- Here we go into the first week of veg and second week since breaking ground for the FastBuds Papaya Sherbet photoperiod! 5/18- Right away what ever has been chewing on my other outdoor plants, has now decided to take a taste of my Papaya Sherbet so I'll be spraying with Neem Oil and adding a ring of Diaotomascous Earth around the Papaya Sherbet to deter any slugs... I don't like slugs! 5/20- Rain showers yesterday handled the watering duties and all I did was to add some straw to a couple of bare spots on the mound. 5/22- There's two weeks on the books for this pretty little photoperiod from FastBuds and I can't wait to see what she'll do in the coming weeks! Thank you for checking out my diary, your positive comments and support make it all worthwhile! 💚Growers Love!💚😎🙏

In week 10 I have some plants, which have grown taller than the rest (canopy is appr. 80 cm high now). In order to not have to raise my lamp, I rather correct their height by 'supercropping' them. Supercropping is a technique developed by SOMA from SOMA_seeds and works VERY WELL. Instead of topping your plants (=cutting off the main stem) it is better to take the main stem between your fingers (appr. 15-20 cm form the top) and brake the fibers inside and then bend it downwords. This way the stem will stay intact and heal itself and you dont loose the main buds growing on it later. In the video above I show and explain how I supercrop a plant. The plants are now getting more and more into flowering and therefore I switch the dimmer on my SANlight EVO4-120 plants to 100% now (see in video above). The BIO NOVA nutrients are increased as well a little bit, so the plants can build big buds.