OK, im doing an update now becouse my breakup cake is going tranny!!(hermie) I already have noiticed she was not growing as strong .. but u can blame that on anything , especially when growing outdoor.. but this morning i saw that she was g(r)oing 'bananas'!😢 I have put her aside from the rest (in the back of garden) but think gonna have to terminate her asap!..(and i will, right after this post👍) The weed she will give will be filled with seeds and those seeds will be hermies too, so wothless.. and if i dont throw her away quick she can infect the rest. So goodbye breakup cake..😢 who wants to eat a cake when they are breaking up anyway..😒 (just hiding my pain with lame humour 😳) happy growing for all ✊

Bien..ya corto el Stretch.. estiramiento a princ de flora..miden 70-80cm ..se estan llenando de tricomas.baje lampara a 50cm del dosel. Fertilize c Tricomas-namaste y con Flora Booster-namaste...en unos dias hago lavado de raices..y luego regare solo c agua c detox..p limpiar raices.¡

Super happy with how things are going for a first grow. I did some LST and regularly bend fan leaves to expose more areas to light. Feeding only water with a tiny bit of liquid fertilizer.

The two main events this week: Defoliation and training. The lady seems to take it well. Apparently the nuts I use have a rather low amount of Phosphorus... I had the same problem in my last grow (dark leaves, slow bud development)... unfortunately that time I didn't get what was happening. So I started giving them "Bud Explosion" now, 2ml/l, which basically contains a mix of Phosphorus and Potassium, and the improvement was almost instantly noticeable! I'm thinking she gets 4-5 more days to recover in veg. then I flip her.

Hello Diary, The third week of vegetation has ended, which is also the beginning of flowering. As you can see in the photos, Milky Way has started to form flowers which is normal for automatics after 3 weeks of vegetation. Milky Way is developing nicely, it grew about 10 cm in a week and got a lot of new leaves. The bright spots that were on some of the leaves have disappeared, which is good news. I started to feel a slight smell, which also makes me happy :). This week I also did minimal defoliation, I removed only the lowest leaves to make it easier to water and improve the air flow through the plant itself. When it comes to watering, it is not frequent, about every 4 days. This week I added one tablet of Easy Grow Booster. The conditions on the farm are excellent I would say, the temperature is around 26 degrees while the humidity is around 50%. Here's how it looked this week. 16/05/2023 - Day 17. Watering. I prepared 7 liters of water, lowered the pH. to 6.0 and dissolved one Easy Grow tablet and with that amount of water I watered all three plants equally. 19/05/2023 - Day 20. First, I did a minimal defoliation, removing only the bottom leaves to make watering easier and improve air flow through the plant a bit. After that I watered all three plants. 20/05/2023 - Day 21. Measuring the height of the plants and taking photos at the end of the third week. Milky Way F1 - 27 cm. That's all for this week, see you soon. Thank you all for your support and comments.

This week was a very easy one all that was involved was flushing. Flushing two separate times throughout the week involved tap water which was allowed to sit for 24 hours with air stones. Tap water was used first because the PPM of tap water is higher than RO water. Doing this avoids instant deprivation of food for plants, rather than cold turkeying them. As the last week arrives tap water will be replaced by RO water equating to 400PPM or less and given in abundance until PPM meter reads runoff at about the same 400PPM or less.

Aaarg same problem😩 Screenshot🔝

She hasn't grown up, but she's filling out quite nicely. Looked at others growing Criteria Kush (CK photos) and noticed the growth of plant just before they put into flower. My husband decided thats what we are doing. He put up a trellis and we will use this to help during flowering. She seems happy and content. I hope she stretches and flowers nicely. Its flower time baby. 🌺⏳

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Been wanting to try out this auto ghost og from original sensible seeds for a while and finally got the opportunity to get one wet. I originally started this setup to compete in the solo cup challenge, but I needed something that will be minimal maintainence and doesn't require hand watering. So this is what I ended up with. I will not be entering since I know it will bring controversy, but I am going to follow the rules and treat this grow as if I am competing. I didn't want to use regular solo cups because last time I had a lot of algae growing in the root zone when I harvested. I opted to use a black light proof cup. These cups were 27 oz originally so I filled them with 16 oz of water, marked the level, then cut them down to 16 oz. I set the drain pipes about an inch from the bottom so they have a little reserve if I have pump or power issues. I know people will see the drains as a way for roots to grow out of the cup but in reality I have to keep the entire pipe free of roots or it will clog and overflow. So I am actually losing a little volume due to this. I filled the bottom inch or so of the cups with river gravel to stop the perlite from washing into the reservoir. I am running a 5 gallon reservoir with a small air pump and the plants will be getting irritated 24 hours a day from an aquarium power head pump. I set the system up to be totally self contained and easily portable. It was all built from things I had laying around other than 97¢ for the cups. I will be keeping the feed simple as always feeding maxigro and maxibloom with a couple flower boosters.

Another week in veg.. she's patiently waiting her turn to get into the other tent where she can start producing fruits ... i'm hoping for a good one with this and I have a feeling it will be.. Thanks again to @Grow4releaf... God bless and happy growing ✌️

The red and purple hues on this girl are a sight to behold, as I upload this part of the grow diary I have already harvested her (stage 1), it was painful to do but I soon got over it! I've enjoyed the surprises she has thrown at me along the way, like growth rate, hight, her sensitivity to environment changes during veg but that was only due to not reading the smaller print and not doing a bit of genetics homework and more "I want to grow Blue Cheeses".. She's a proper smelly lady and I actually didn't like the scent (never thought I'd say) that she gave off when rubbing against the main stem and branches whilst LSTing. Conversely the flower was yummy, a piercing sweet blueberry note followed by the chonky cheese stink, a definite "Grow again Jane" so far and any I've not even smoked any yet!.

Welcome Back!💚 Die zweite Blütewoche ist vorbei und die Pflanze entwickelt sich weiterhin prächtig. Die Blütenansätze sind schön erkennbar und die Pistilen bilden sich weiter aus. Überraschenderweise wirken die Blätter sehr Sativa lastig, wobei genetisch eine Indica Dominanz vorherrschen müsste. Außerdem ist ihr Wuchsverhalten eher Indica dominiert. Jetzt ging die Pflanze etwas mehr in den Stretch und ist mit ihren Indica dominanten Mitbewohnerinen gleich auf. Die Werte im Zelt sind optimal auf die Blüte eingestellt. ——————— 🌞 Temp: 24°C 🌚 Temp: 20 °C 💨 RH: 56% VPD: 0,97 kPa 😎PPFD: 830 mqm ——————— Stay Tuned! 💚

How is it?

Flushed and final week

Hello Diary, I am truly privileged to be one of the first to try growing this revolutionary new strain. My dear friends at RQS sent me this new hybrid seed at the beginning of the year and I'm really looking forward to seeing the result. Let's see what they say about this new strain. Medusa is a true F1 hybrid created from pure, inbred cannabis lines. She boasts uniform growth traits, mouthwatering aromas and flavors, and plenty of potency. Combining genetics from inbred lines deriving from Sugar Magnolia, a thick and sweet indica, and American Beauty, a fast, fruity hybrid loved for its well-balanced, positive high, the result is an autoflowering F1 variety that produces fresh flavors, a unique cannabinoid profile, and large yields. Medusa F1 seeds produce elongated autoflowering plants with long, strong branches and well-spaced internodes. Plants regularly grow up to 80–85 cm tall and boast a fast flowering time of just 42–45 days (72 days from germination to harvest). Thanks to her genetics, Medusa F1 produces plenty of thick flowers with big, swollen calyxes that make for very easy trimming. Medusa F1 is very stable, handling stress and unfavorable growing conditions with ease, although she is somewhat susceptible to Botrytis, so make sure to keep tabs on the humidity in your grow room/garden. SET-UP ON MY LITTLE FARM: Box - Secret Jardin DS120W 120x60x178 Lights - MIGRO 200+ Ventilation - TT Silent-M 100 Filter - Primaklima filter PK 100/125 Fan - Oscillating Koala Fan X 2 Humidifier - Beurer LB 45 Soil - BioBizz Light - Mix Pot - 11L Air pots Seed - Royal Queen Seeds Nutrition - RQS Organic nutrition LET THE DIARY START OFFICIALLY: 12/01/2023 Planting. I prepare everything I need for planting in advance. I fill the AirPot to a little more than half and add 50g of Easy Boost Organic nutrition in pellets and mix with the soil, then add more Easy Roots Rhizobacter and Easy Roots - Mycorrhiza mix and fill the pot to the top with soil. After that, I soak the soil well with water, make a hole in the middle and carefully insert the seed into the hole with gloves and cover it. After doing this with all three pots, I placed them in the grow box and waited for them to sprout. The lights are 35 cm away from the pots. The humidity is not high, but as soon as the plants sprout, I will put in a humidifier. 14/01/2023 I moistened the soil a little with water. I need to keep the soil moist. 16/01/2023 The first plant has sprouted. We are still waiting for the other two. 17/01/2023 The second plant has sprouted, but I'm still waiting for the third. 18/01/2023 Unfortunately, the third plant did not sprout, so this time there will be only two plants on the "Farm". 21/01/2023 First joint watering. Each plant received about 1 liter of water. That's all for this stage, see you soon and thanks in advance for your support.