2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

She is almost ready the buds are getting dens and covered in crystals

hello everyone I had a very positive week on my BSB Bruce Banner colas are forming and hardening full of resin are on the flowers and leaves the harvest is coming soon I am very happy good luck everyone 😘😘💪💪👌👌👍

Day 168- 20/11 : She is still swelling some more and the scent is getting stronger and stronger ! An amazing spicey, chocolate smell with hint of berry. When really rubbed the scent turn into a very strong hazey sweet hash scent . Just amazing. The buds are starting to fall over and so I starter to support them. I think she will be ready within the next 5-7 days. Day 170 - 22/11 : Looking good ! She is fading nicely and buds are maturing, Harvest is so close I can taste it !

Checked the two unsexed bagseeds again this week. I was correct, I had one male and one female. I have moved all the younger flowering females into the larger 4x2 tent and moved the male plant and the older purple trainwreck plant into the smaller 3x2 tent. I am hoping to isolate the male and minimize pollination of my girls. This is an end grow, I want both bud and seeds. My plan now is to minimize pollination through isolation and pruning. I want to pollinate a single branch on each of the females, this way I only have a few seeds from each plant and still have quality bud. The male has been trimmed back some already and the pollen sacs have not burst yet. The older purple trainwreck plant is adding weight now to the buds and should be done in approximately 2-3 weeks depending on trichomes. In the larger tent, the back left corner is Pineapple Express. It is the tallest plant in the tent. The front left plant is white widow. The middle front plant is also white widow. The middle back plant is a bagseed, I suspect berry white still for this plant. The far right plant is the younger purple trainwreck plant. Currently feeding the younger plants every 5 days or every third watering depending on the plant. The older flowering PT plant will get its last feeding this week and will start a flush after that. Happy growing 👩‍🌾🏼

Flowering nice. Madarin Cookies are two in back and one in center Silver Kush are two up front. I ran out of room for scrogging branches in back, letting them just strech. Unfortunately that has me keeping the light higher than Id like. Since S.K. are shorter, the one at right side is 30" from light, while stretched branches in back are 12" from light. Averaging 18" overall from lights.... Hope i dont get light bleaching! UPDATE: Added 2nd screen on Day2 to keep stretchy branches lower Cookies are flowering nice, one of the silver kush is just starting to flower while the other hasn't started yet.... hmmm 2-3 gallons water/nutes per 5 plants, alternating with plain water (just calmag, malassis, Ph. 6.4) every other day. Ph.6.4

Adding SLF 100 and Calmag to feeding. Although everything seems good, I'm going from tap to RO to have maximum control over my PPMs or TDS. Buds are really developing nice and she is still stretching. I appreciate any suggestions. ( Running CalMag, Photosynthesis Plus, and SLF 100)

1 moth passed from germination. In result we have 12 strong plants and 4 reduced. They begin presenting wonderful scent! Increased my led on 40 % of power and it seems great. Buen fin de semana a todos !

I had a great feeling of accomplishment as I harvested Daphne today. She was the first plant I have brought to life in many years and I was impressed with how the A.B.L.E. Grow Chamber worked. I will let her dry out for a week then I will update more.......It has been a week and I am more than happy with her smoke. VERY SMOOTH no harshness. Great after taste. Even though she was a small plant she still did extremely well in all other factors,: great color, no disease, good tie down technique made for 6 tops with one main one. I will use a single 5 gal bucket for my hydro next grow to see if I get better root growth than with the 6 plant reservoir I used for this operation. The grow chamber allowed me to control the humidity, air temp, pH and PPM with relative ease. I am very happy with my first grow operation with the ABLE Grow Chamber and hope my diary was informative and entertaining for everyone. Main thing i did wrong was my fuck up with keeping the LED too close when she was a seedling. I had it at 10". (it was my 1st time w LEDs.) Secondly, I never got good root growth but not sure why. my theory is that light was seeping through the white plastic cover on the Rainforest 66 reservoir. The best thing I did was controlling all the factors needed to grow with my A.B.L.E Grow Chamber Prototype. It made great use of limited space and saved electricity with its low power usage. Look forward to using it one more time before we make the first salable model. 0

Finally back to it!

Harvest Day 100 Strong Tropical Aroma

5’-2” & 4’-6”!! Growing like weeds!🤣👍💪 Loving being outdoors and the high temperatures we’ve been having! Supplemental Recharge and Cal/Mag added once per week.

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More pictures to come as this week begins to unfold! Expecting more rapid growth during weeks 3-6. The Bride Cake is the leader for height and daily progress, seems the Dosidos will play a game of catchup during these next few days. 9/29/21 9:44pm I’m noticing a potential calcium deficiency on the bride cake. Bumped up the nutrients to 1.7 EC from 1.1 since they’re picking up in growth rapidly.

She had a tough life, I messed up her roots at the beginning and then overwatered, was really stunted and not growing for a while. Finally started growing normally, a bit late but looked fine. I had to hand her over to a friend for the last 4 weeks and she looked strange and buds were quite solid hard and dry.

Twenty20 Ateam R&D Update! Wow this week went so great, Germination was 100%successful!! We went with the usual paper towel method an they all popped up in the soil on Saturday the 28th! Let’s grow lil babies let’s grow!!

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The stretch phase is officially over, and now the plant is shifting its energy toward trichome production!🌱 I’ve started noticing a light scent developing, though it’s still not very intense yet. I expect that to change in the coming weeks. I did a light defoliation to improve airflow and light penetration, and I plan to do another round in about two weeks. Interestingly, the fan leaves are starting to take on a slight reddish hue—curious to see how that develops! 💪😊 So far, everything is looking great, and I’m excited to watch the buds bulk up in the next phase!