7/21/21: WEEK 6!!!!! She's looking pretty good and I'm super impressed with how well she has handled the heat, she's doing way better than some sativas we've grown indoors during summer..I think she'll start plumping up this week..we cut growth solution roughly a week ago but she's still pretty dark, I imagine there's a bunch in her media to use up yet.. we noticed a split starting down her main stem so we've released some pressureon the ties and wrappeda plant tie around the base temporarily... we'll update again midweek, thanks for reading and happy growing friends! 🐱❤️💡🌱 7/23/21: hairline split down the main stem has healed already, that's good news... buds are gradually starting to fatten up.. we're still around 85-90f during the day so we may consider reversing our light schedule to run at night but this is something we would do after the GDP is harvested, the other 3 plants in the flower tent are in week 3 and week 1 respectively and would probably deal with the light disruption a little better (just a guess but no sense in rocking the boat this late in flower..ty for reading friends 💓 7/26/21: She's starting to put on some weight 💪😺.. we went camping for the weekend and her buds were noticeably thicker when we returned 2 days later..I uploaded a short video ❤️🐱💡🌱

WE HAVE MITES. I was looking at trichomes and I saw one walking. It looks like they are not spider mites but they are eating the leaves……. I bought predator mites they last 2-3 weeks I will take them out next week so they can die and they can get washed in the trim process. The Sensi bloom I powder so I am feeding 6.1 grams in the solution. This solution is about 11 Liters per day. The buds are exploding. You can see them pop daily with this overdrive. It is truly the magic in the end the buds get super hard, and THICK.

A lot more active on Instagram, green_house_lab If interested in the genetics hit me up

~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 1/29/22 😿 It's been a interesting week, we got some of our color back but I think the old peat in our media is dragging PH down which is likely the cause of the lockout seen one a couple of the girls, we intend on flushing 2 plants later today (we almost never flush) with 3gal each PH'd to 7-7.5 with a mild leaching solution in the last gallon. I really, REALLY hate making PH changes this late in flower but we gotta do what we gotta do...it's definitely not the worst i've seen but if we let them ride like this it will absolutely impact our yield..we can't have that folks lol... we'll update their progress midweek, thanks as always for dropping by and happy harvests everyone!!!! 💚💡🌱😽💨 ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 1/30 😼 we gave the monster Big Bud (not seen) sharing the tent with these girls a good monster cropping today, this allowed us to drop the lights a little over 6inches today ❤️💡🌱.. ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~

- introduced 7W far red light bulb to boost stretching (girls are VERY bushy) - slowly drying soil (so I drilled more holes on side of the pot like a cheap dyi airpot) - preflower Keyra (the bigger one) is what I would say a stretching phase and Margot (smaller) evidently falls behind but not stretching yet, I hope she catches her atleast little bit..

Alguien sabe algún remedio para los trips

Rough week, had to flush the bigger plant to fix lockout. Did 15 gallon flush with ph balanced water, the last bucket of 5 gallon had 50% strength nutrient, minus the flora Micro which I will stop using for the rest of the grow. I installed my scrog net, only about 30% of the net is filled. The second smaller plant doesn't even reach. I sent the height to 18 Inches from the base of the plant. I lose 10 inch from pot I also lose 18 Inch from light and carbon air filter 6ft tent max I lose 2.5 ft. Only 3.5 ft to grow. Going to increase nutrients to 75% strength and only give cal-mag 1x a week as the support team told me to do so. Heavy trimming and defoilation. I will spread the plant and any branches wanting to pass my screen will get topped until the tent is filled. I took 5 clones from each plant, some of the thin long branches that were not getting any light. I am starring my first dwc grow at the same time in my 2x3 tent with my gf. Unsure if I will have a small soil run the same time. The plant is starting to smell strong, honestly like stinky armpit mixed with cat piss. I am not turning on carbon air filter yet, I like keeping it humid during the veg cycle and I keep RH around 40-60% when I turn on carbon filter.

This week was the last week of veg., I will switch to 12/12 (light/darkness) in week 6. The two Barbarian (=AK-47 x Barbara Bud) plants are now 35 and 40 cm tall and have developed nice side-branches, which have grown upwards so they get enough light for good budding sites during flowering. The plants are still fed following the Advanced Nutrients schedule.

2/4/19 - I'm really pleasantly surprised by Tangie'matic. I expected stress and stunting after topping, but she's pushing back with vigor and growth! I'm relieved, as this is my first topping experiment, and I'm doing it with an autoflower. Naturally, learning something new comes with a certain degree of uncertainty, but I feel like Tangie has been more of a partner in this learning process. For that I am thankful. 🙏 2/6/19 - Reservoir Δ Day! Tangie'matic's roots look immaculate!

She was great off the start ! Nothing bad to say except I only got 1 seed of this strain :( I wish I had more loll

Day 28-35 (May 28th - June 4th) (Day 29) My rooted blackberry #3 clone looks like it’s finally starting to reveg. It’s starting to stretch it’s internodes but no new growth yet. Nearly all 9 are pushing roots now. What a waste of time taking clones in flower! Won’t ever do that again. Grapefruit #1 has a twin! I thought it was just an odd formation on the taproot but two seedlings are pushing out. I wonder if it will be genetically identical. It’s definitely slower growing than the other one so who knows. I will try to train them apart when the time comes. (Day 30) I think the EC/PPMs are too high on nearly every plant including my veggies. The promix is actually really hot for a “blank” medium coming in at 1800. Adding compost and amendments to that is probably raising it to 2500+ at least. It should self balance the more I water it but I know for a fact I can get better growth by leaching the soil around each plant. Next time I use promix for anything, I’m going to prewash it like coco coir and add a dash of oyster shell flour for pH and calcium. (Day 32) Purple tuna is now big enough to start lst on now. I’m going to wait a little while for the slurricanes. (Day 33) One of the grapefruits has a twin that actually popped... I’m going to leave it and see how she turns out (Day 35) I’m going to prune off the lower leaves on each plant. I was going to lst Purple tuna but it’s so vigorous I may as well just top/mainline it. Autoflowers are looking established and should be taking off this week. I wonder if this will give them enough time to veg into decent sized plants before they flower. I won’t be topping or even doing lst. My goal is 22 big spear colas from each plant. I’m going to get all my rooted clones into 1.7 gallon pots over the next week or so.

Plants are looking great. Have really filled in nice. Can already tell they are going to stack the bid on. Gave them a good lower trim a few days ago. I got a new bigger tent and was going to move them but decided to just finish them out in the old one. Really excited see What the next few weeks bring. I’ll update soon. As always 🍻

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Day 35 from seed, Last week the lady's have been growing like crazy😁 i guess they are well established now in there forever home's, also the temps have been dropping quite a bit over here. The week prior i had big troubles getting the temps under 84f at all times but now they are stable again @ around 75/78f lights on and around 70 lights off. Btw upcoming week i'm gonna do a big defoliation and some lollipopping to get them ready for flower🌺🤤 Stay elevated growmies 💚

She's come along way now in the flush and hopefully chop this week, shes swealling now and oh my does she stink so gassy yet fruity, remember happy growing and stay green 😀 💚✌️

How the time has flown by. She completes her flush this week and will start her dry, Sticky, smelly and buds are thick. Extremely happy with how she handled these last few weeks! Excited to taste her.

Done..... 19 plants Tangerine Skunk I love this pheno 🤩 🍊🔥🍊🔥🍊🔥🍊🔥🍊🔥🍊🔥🍊🔥 😈

Last week went well. She seems to be done with the major part of the stretch and is stacking on bud now. I upped the cal-mag a mL per gallon because of the light intensity. Had to adjust the light upwards to avoid damage to the plant. I began to see some signs so had to take care of it but she is happy with the environment. She is growing some nice long buds just like the gelato next to her. Glad I have clones from both. Had to do some supercropping and defoilation. Did it to keep the branches at a good level and to keep the canopy level. She would have been taller if it wasn't for the lst. Will update as she progresses. Until next time you growing to everyone!