My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

The flagship of all my grow rooms, this is the strain you want to run into constant production I find strains the pack on the sugar, but will not bulk up and chunk up, in my option Seed Junky Genetics always delivers top notch seeds

Everyone got transplanted yesterday! They've been hardening off outside for almost a week. The got some Fish Emulsion a couple days ago. One night it got down to 38F, even though the weather said it would only get down to 50F! So I'm very grateful that they're so resilient. They're not showing much transplant stress so that's good. I watered in some lime when i transplanted. I put them in with my tomatoes that I need to stake. I started putting down seaweed mulch which hopefully I will finish tomorrow. Overall a good week and it feels good to have them in the ground!

Jesus! Smells like pineapples and some sort of exotic juice. Amazing. Definitely coming from the purple afghani lineage side They’re chugging along nicely, will switch to the 10/14 next week and see if that helps with the ripening phase. Sticky as hell, just brushing against them will feel like glue on your skin. Sticky, frosty and stinky!

Starting to look good. Taken out the Grow food. Not a lot to do😎😎 Started to think about what to do next, will be a full 4 pot tent tho. Got a nice tin full of different strains, poor me, the choices I have to make😂🤣😂🤣

The flowering continues, only on one branch I went wrong I think the bud will not grow .. The girls are suffering from N deficiency (?) Or from continuous changes of Ph (?) Who is more experienced tell me .. Hope flowering finish well, I add some N. Besides, the heat is advancing and the temperatures in the grow are rising a lot, fucking summer

Week 7 light time 12/12 from now 🍀✌️ Day44Little more lst and defoliation 💚✌️

Grow report week 8: The legalization and the Tent-X System Hello dear growers and groweresses, it’s time for an update on my grow. My plants have completed the eighth week and have continued to grow. But they have also experienced some exciting news, which I will tell you. The legalization This week something incredible happened: The Bundestag passed the partial legalization of cannabis. From April 1, cannabis consumption for adults is to become legal in Germany. This means that I and my brothers and sisters no longer have to hide and can freely pursue our hobby. I thank everyone who helped us achieve this goal. We will not stop until you also get your right. Legalize it everywhere! I am very happy about this decision and hope that it will contribute to a better health protection, more education and prevention and a stronger child and youth protection. I am curious how the new drug policy will affect the grow scene. The Tent-X system This week I also got a new system for my room: The Tent-X system from TrolMaster. The Tent-X system is a state-of-the-art, professional and modular environmental and irrigation control system designed for single-zone grow tents. It allows me to effectively control the environmental parameters and irrigation schedule of my tent with a single control system. I think the system is outstanding! It offers me many settings and functions to automate my system according to my wishes. For example, I can change the lamp height or power based on PPFD values, read the substrate moisture and EC value, simulate the sunrise or adjust the light spectrum. The system is also very easy and intuitive to use. I can control it via my smartphone and access my data at any time. I am happy to have this helper for the upcoming flowering weeks. I am sure that he will help me to a better yield. I am also very grateful to TrolMaster for providing me with this system. It is a great honor and a privilege to be able to test and use such a high-quality product. Thank you very much, TrolMaster! You are awesome! 🙏 The plants This week my plants have grown vigorously. The back four will all reach their net next week. I defoliated them heavily in the lower area two days ago, which they tolerated well. They look really healthy to me. Taking pictures is very complicated right now, you hardly know where one plant starts and the other ends. But that will get better in the next few weeks. I am happy that it is now going into the last vegetative week and we will reach the flowering on next Sunday with a light cycle on 12/12. The outlook I am very satisfied with the result of the eighth week. My plants have developed well and are ready for the flowering phase. I am very excited about the next few weeks, where I hope to harvest many beautiful buds. Thanks for stopping by and see you next time! 😊

Harvest day 70 since time switch to 12 / 12 h Hey guys :-) Finally it's time 💚 The lady is done the large leaves have been removed and hung upside down to dry in the dark drying room. You can now stay there for 13-15 days at a temperature of 18-20 degrees and 55-62% humidity. After 13-15 days it is neatly trimmed by hand and placed in jars with boveda packs 62. After 4 weeks Boveda 58% come in and are ready for testing ;-). After everything has been cut cleanly, the last update comes with the smoke report and the finished pictures. Let's get to the plant 💚. First of all I would like to compliment Green House for this genetics. Unfortunately, the rating system at Growdiaries is a bit strange because I have to give the stars before the Smoke Report and in the end it might have led to more stars in terms of taste. The growth was great from start to finish. She had no problems at all and also had no problems with animals 👍. I'm amazed at the great smell the beautiful buds give off😀. Of course I cut cuttings and if the taste is as good as the smell it will be grown again ☺️. A final report comes with the Smoke Report. Until then, I would like to say thank you to the whole Green House team and wish you all the best fun with the diary 💚🙏🏻 Have fun and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 ‘Powered by GreenHouse Feeding’ Copy the link for 10% off all Nutrients 👇🏼 http://shop.greenhousefeeding.com/ affiliate/madelngermany_passiongrower/ 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 Water 💧 💧💧 Osmosis water mixed with Cal/Mag (24 hours stale that the chlorine evaporates) to 290 ppm and Ph with Ph- to 5.8 - 6.4 MadeInGermany

Finally got my buddy to come out and add in a 20 amp outlet so I can fire up a better light. Still using quantum boards but with a 4500k spectrum. Got in a foliar with the Growzyme from green buzz liquids. Fed them right before I took the pics they look a little sad but hopefully they bounce back quickly. Not much else going on. The code bangdang will get you discounts at.. Gorillagrowtent.com Rainsciencegrowbags.com KindLEDgrowlights.com and more (see Instagram Bio for @bangdangbuds) Also check out my Instagram if your interested in entering a giveaway for a green buzz liquids starter kit including some living organics, fast plants spray and some seeds of the winner choice.

strated with mainlinning

Finito

we just finished 2 weeks are are going into the 3 week. These ladies can EAT!! 1,000 ppm’s and no issues..looks like I have to up the ppm’s for bloom phase 4 out of the 5 are doing GREAT. The 5th one is having issues and I know it’s the media I am using. I had a different brand of soil for 1 of the banana MAC’s and it freaking SUCKS!! I am amending with Iron-Tone by Espoma to get the green back and it has some cal-mag also with sulpher ( hits all suspect deficiencies). The other 4 are in Mother Earth soil which I have had zero issues with. I will be sticking with Mother Earth for awhile. 11/6 Potassium deficiency in the banana MAC’s…kinda of a good sign. Let’s me know the plant is going into bloom phase and it’s time to change the ratios from 1:1:1 to 1:3:2. The 1:3:2 is flower girl 1 week and fish bone meal with Langbeinite the other week. I will alternate between the two for 4 weeks. Tuesday ( 11/8) will mark 3 weeks. I did defoliation and lollipoping today. This is the second one I have done ( I do the first one the week before flipping).

Haven't uploaded in a month, but back at it! Plant has been doing great. An animal almost cracked the main stem right through but with some tape and patience she recovered quite well. Recently discovered that i should water her even more (first plant) so now i'll give her a good 2-3 liters every 2-3 days. She's standing outside 24/7. I've also ordered General Hydrophonics nutrients yesterday since i had the opportunity. I've topped her once about 2 weeks ago and I'm thinking of topping the 2 main stems again soon. Wondering when she'll go into flower though See you next week!

lowered the ionbeam lights and adjusted all lighting to get even 20k lux everywhere.

Que hay familia, vamos con la novena semana de floración de estas Apple Fritter de Zamnesia. La temperatura está entre los 21/24 grados, la humedad está entorno al 50%, y el ph lo mantengo ahora al principio en 6.2, el foco por supuesto está enchufado 12 horas , estan creciendo bien fuertes. Todo correcto hasta ahora, ya van madurando las flores y ya están bien resinosas, estas próximas semanas veremos cómo avanzan, ya quitamos la gama de alimentación y ya esta semana estuvieron con agua solo. Os comento que tengo un descuento y para que compréis en la web de Zamnesia de un 20%, el código es ZAMMIGD2023 The discount 20% and the code is ZAMMIGD2023 https://www.zamnesia.com/ agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo, buenos humos 💨💨💨.

Memes for the fastbuds meme contest Memes for the fastbuds meme contest Memes for the fastbuds meme contest Memes for the fastbuds meme contest

first week of flower. shes looking good. 👍 20/4 - 550-600 ppfd. reading done with photone, using paper diffuser, close enough to what i was imagining it would be, with 75% at 16’. d.45 - she grew 3 cm over 24 hours, thats pretty impressive! d.48 - light defoliation in the bottom