7/20: All three are doing great. I'm hoping to extend Plant 1's vegetation week so that the others catch up to her for the flip, which is eventually coming. I added RapidStart this week. I've heard great things, we shall see. More root, more fruit. Trimmed 2 lower fan leaves that were not getting any light. 7/21: Our time is drawing near for the flip! Are those........ STIPULES? 7/22: LSTing hard. Much good. Nutes niiiice. She's just letting me bend her all sorts of ways for that light. 7/23: LST for you and me, to grow some weed so happily... la la la 7/24: They are just perky tonight! 7/25: Last day of week 4. I started some bloom nutes this morning to prep, and tomorrow I lollipop 'em! I also got a new microscope for the trichomes later on, can't wait to see what's photos and videos I get.

Well after different bits of advice last week I decided to take fast buds advice and keep feeding, she's on day 70 now all her leaves are fading, I'm guessing this is just because she is coming to the end of her cycle,.. still not sure when to start flushing as fast reckoned there was 3-4 weeks left that was 7 days ago so now still 2-3,.. I reckon 1 more week of food but just what I've put on the nutrient list nomore and then start a flush, just to say I appreciate all and any advice given to me I just followed fastbuds advice as I thought probably safest option. Thanks everyone. Roll on next week 👌

Ehi ragazzi è già passata una settimana e neanche me ne sono reso conto! Si vede che l erba che sto fumando è bella potente per farmi rimanere in uno spazio/tempo non ben definito! Apparte questo divagamento, posso dire che i nutrienti stanno facendo effetto! La ragazza sembra che stia crescendo davvero bene , ogni settimana raddoppia le dimensioni circa , ora siamo già al quarto paio di foglie laterali e ho intenzione probabilmente in settimana di mettere la rete per iniziare a direzionare le piante,in modo da avere più cime possibilmente uniformi, ma soprattutto, avendo il problema dell altezza, cercherò di fare in modo che rimangano più basse possibile , dovrò studiare un metodo per farlo senza stressarle troppo e senza utilizzare tagli e toppature che in un autofiorente,necessiterebbero di troppo tempo per recuperare dal taglio e quindi la resa sarebbe minore,... Bene siamo solo all inizio e non vedo l ora di vedere come cresceranno queste ragazze magiche ! La luce FC E 300l è davvero impressionante! La sto usando al 50 % della potenza e devo dire che stanno crescendo in modo molto rigoglioso tutte le ragazze in modo uniforme anche ai lati, la luce è molto ben distribuita rispetto alla TS 1000 che avevo in precedenza , che nonostante tutto ha fatto il suo dovere e lo continuerà a fare in modo più che egregio! Buon 420 a tutti alla prossima settimana! 💪👊😉

Week 11 - Day 1 : Pinched branches from subject 1 and 2. As seen in the videos uploaded. Week 11 - Day 2 : Changed water schedule to water-water-feed, watering my plants with 4 Liters per plant every 3th day. Pinched branches recovered successfully. Week 11 - Day 3 : Pinched lower branches of subject 1, take a good look and tell me what you think! Week 11 - Day 4 : Pinches branches of subjects 2 and 3, take a good look and tell me what you think! Week 11 - Day 5 : Pinches branches of subject 1, take a good look and tell me what you think, had my first harvest of ladybugs in the garden and released them on my plants, noticed that I have very little spider mites on my plants. Posted week 1 to 9 on my diary. Week 11 - Day 6 : Woke up to subject 5 being damaged, What could have caused this? Too heavy branches? Maybe an animal that accidentally broke the branch and took off. Week 11 - Day 7 : Pinches branches of subject 3, take a good look and tell me what you think!

So Ms. Divine OG is thriving she is very healthy and strong 💪 started flowering stage and introduced flowering nutrients! Cant wait to see her development!

🤯😎🤙🙃

Back to normal now growing like a beast crystal is forming nicely

23.05. today is the end of the fourth week for the plants, they haven't progressed much since last week, they grew a few cm and gained a little weight, but they are healthy, which is the most important thing... in a few days I will transplant them and top them because some of them have already developed the sixth pair of leaves, so I simply have to top them. turned out... I thought of transplanting them into 10-liter pots and after two to three weeks into 50-liter holes, however, the weather didn't cooperate, and I was already late anyway because of the bad weather, and because of the same, my plants in the fourth week were the same as they should have been in the second, and because of the same, I couldn't even transplant earlier... now that nothing went according to the "plan", of course, I don't know how smart it is to transplant them into 10 L and then into 50 with the fact that it is with me the longest day of the year is June 22, so that actually a week after that it can start flowering (it usually starts at the end of July or at the end of August, but it can be late and start even in September, and it also knows how to plow and start already at the end of June)...now I think that it is better to buy smart pots of 50-60 liters and immediately transplant into them and then just bury them...another problem with that is that the soil that I took for vegetation has food in it for a month days, if I transplant twice, it may happen that it enters flowering, and the food for the vegetation has not yet been used up, and that would be a problem...advices are welcome Stay high!!!

Hun er virkelig blevet en stærk lille pige 😊😇👌💪

🌱 : Preflowering 💧 : 6l 💡 : Dli: 40 mol/m²/d 🤔 :

All ready for the switch! Will transition on Friday to 12-12 They have their very own shade 😍 can tell it’s going to be a colourful piece of art. had to train the whole tent to acquire every space! Now I think I’ll have a lovely sea of green during transition! Luckily I’m prepared!

Got these transplanted to the rain science 3 gal bags and moved to a 4x4 in the basement where it's a little better environment in terms of vpd. I had a full bag of roots organic soil so I decided to do a soil/coco blend like I did with the current autoflower crop. I also topped every single one of em mid week. Once I get the flower tent all cleaned out and everything I'll be throwing these in there for bloom. Hoping this will be an even better grow than the last since I have both my arms back. I'm doing the update a little early since I was late on it last week, today (tuesday 9.10.19) is day 43 from germination and about day 39 from full sprout. Before this week they were under only about 185w in a 4x4 area so it's been slow growth so far. The hulkberry is doing great it's one of the most rapid growing and overall healthiest plants in the crop.

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Tb

Lets' go 24/01/25: All good. Gave ph regulated water, no nutrients. 25/01/25: All good. Comments / tips concerning pruning are welcome, but I tend to wait a bit more.

Day 64. Watering with fertilizers. Day 65. Watering with clean water. Day 66. Watering with fertilizers. Day 67. Watering with clean water. Day 68. Watering with fertilizers. Day 69. Watering with clean water. Distinct sweet smell. Day 70. Watering with fertilizers.

Am Wochenende haben wir das Netz gespannt. 2/3 der Blütezeit hat sie jetzt ca. hinter sich gebracht. Nach dem ausharren und dem neutralisieren des gekippten Wassers, trat sehr schnell auch ein Mangel sehr vermutlich ,,Schwefel,, am Haupttrieb im obigen Bereich auf. Da wir aber so oder so in der ersten Woche mit erhöhtem Mineralien bedarf (Top- Max) uns befinden, ist dieses nach dieser Situation ein absolut positives Anzeichen, da es bedeutet das sie die Nährstoffe benötigt und somit auch verarbeitet. Auffallend und auch nicht mehr zu ignorieren sind die Thripse. Um diese kümmern sich aber ab Paketeintreffen die neuen Florfliegenlarven. Ansonsten haben wir beim Netzt Spannen noch kleine nachwachsende Stins weggenommen um wirklich alle benötigte Kraft in die Cola´s zu bekommen. Wir Gießen im Moment ca. alle 24 Stunden 4 Liter Nährstofflösung inkl. 0,08 g Co2_ Tablette und 4 ml PK14/15 Wir wünschen allen Growmies viel Erfolg und eine angenehme Woche! Seit dem 16.10.24 wird Sie nur noch 11 Stunden beleuchtet und statt 4 Liter mag sie im moment eher wieder die 3 Liter pro Tag Nährstofflösung.

09/07 - Reveg still in process - Mother plant in recover from clone clipping - unhealthy clones disposed - cutting new clones this week - Myco and Superthrive feed increased - Net dropped an LST.