7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!""" 7/31 245am Adding a nitric oxide (NO) capsule containing L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), and beta-alanine (40 mg) to the existing mix of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, L-glycine, and methylene blue (MB) for cannabis plants introduces compounds that primarily influence nitrogen metabolism, stress response, and potentially secondary metabolite production. These components are known for their roles in boosting nitric oxide production in animals, but their effects on plants, including cannabis, are less studied. Below, I’ll outline the potential effects of each component in the NO capsule on cannabis plants, their interactions with the existing mix, and the likely outcomes, drawing from plant science principles and inferred mechanisms due to limited cannabis-specific data. ### Effects of Nitric Oxide Capsule Components on Cannabis Plants 1. **L-Arginine (200 mg)**: - **Role in Plants**: L-Arginine is a precursor to nitric oxide (NO) in plants via the nitric oxide synthase-like pathway. NO regulates growth, stress response, and secondary metabolism. In cannabis, arginine (50–500 mg/L) may enhance root development, flowering, and stress tolerance by modulating NO signaling. - **Stress Response**: NO from arginine mitigates oxidative stress from drought, salinity, or heavy metals, potentially protecting cannabis in harsh environments. - **Secondary Metabolites**: NO influences terpene and alkaloid synthesis, possibly increasing cannabinoid (e.g., THC, CBD) or terpene production in cannabis buds, though direct evidence is limited. 2. **Arginine AKG 2:1 Extract (200 mg)**: - **Role in Plants**: Arginine alpha-ketoglutarate combines arginine with alpha-ketoglutarate, a TCA cycle intermediate. In plants, it may enhance nitrogen assimilation and energy metabolism. Alpha-ketoglutarate could support carbon metabolism, boosting photosynthesis and growth in cannabis. - **Synergy**: Provides additional arginine for NO production and supports metabolic pathways, potentially amplifying arginine’s effects. 3. **L-Citrulline HCl (200 mg) and L-Citrulline Malate Extract (100 mg)**: - **Role in Plants**: L-Citrulline is a precursor to arginine in the urea cycle, indirectly boosting NO production. In plants, citrulline (50–500 mg/L) accumulates under stress (e.g., drought), acting as a nitrogen storage compound and osmoprotectant. In cannabis, it may enhance stress tolerance and nitrogen use efficiency. - **Malate Contribution**: Citrulline malate provides malic acid, which supports the TCA cycle and photosynthesis, potentially increasing cannabis biomass and energy for growth. - **Cannabis-Specific**: Citrulline may improve root and shoot growth in hydroponic systems by enhancing nitrogen metabolism. 4. **Beta-Alanine (40 mg)**: - **Role in Plants**: Beta-alanine is a non-protein amino acid and precursor to pantothenate (vitamin B5) and coenzyme A, which are critical for lipid and secondary metabolite synthesis. In plants, it may enhance stress tolerance by supporting metabolic pathways. Its role in cannabis is less studied but could indirectly support terpene or cannabinoid production via coenzyme A. - **Limited Impact**: At 40 mg, the dose is low, so effects may be minimal compared to other components. **Application Notes**: The NO capsule components (total 740 mg) are typically dissolved for human consumption, but for cannabis, they’d be diluted in water (e.g., 740 mg in 1–10 L, yielding ~74–740 mg/L). A lower concentration (e.g., 100–500 mg/L) is safer for foliar or root application to avoid nitrogen overload. ### Synergistic Effects with EAAs, Proline, TMG, Glutamine, L-Glycine, and Methylene Blue The NO capsule components may enhance the existing mix’s effects on cannabis plants: 1. **Growth and Biomass**: - **Arginine/Citrulline + EAAs/Glutamine/Glycine**: Arginine and citrulline boost NO production and nitrogen metabolism, complementing EAAs, glutamine, and glycine’s roles in protein synthesis and nutrient uptake. This could enhance cannabis vegetative growth, root development, and bud size. - **Alpha-Ketoglutarate + Glutamine**: Alpha-ketoglutarate supports the TCA cycle, amplifying glutamine’s role in nitrogen assimilation, potentially increasing photosynthetic efficiency and biomass. - **Beta-Alanine**: May support lipid metabolism, contributing to cell membrane formation and bud quality, though its low dose limits impact. 2. **Stress Resilience**: - **Arginine/Citrulline + Proline/TMG/MB**: Arginine and citrulline’s NO production enhances stress signaling, complementing proline and TMG’s osmoprotectant roles and MB’s antioxidant effects. Glycine’s glutathione production further bolsters stress tolerance. This mix could significantly improve cannabis resilience to drought, heat, or high light, reducing leaf wilting or nutrient stress. - **Cannabis-Specific**: Ideal for outdoor grows or high-intensity indoor setups where environmental stressors are common. 3. **Secondary Metabolite Production**: - **Arginine/Citrulline + Glutamine/TMG/Glycine**: NO from arginine and citrulline may upregulate pathways for cannabinoid and terpene synthesis, as NO influences phenylpropanoid and alkaloid pathways in plants. Glutamine, glycine, and TMG support precursor synthesis (e.g., GABA, purines, methylation), potentially enhancing THC, CBD, or terpene yields. - **Beta-Alanine**: Supports coenzyme A production, which may aid terpene synthesis, though its low dose may limit impact. - **MB Synergy**: MB’s photosynthetic enhancement could provide energy for NO-driven secondary metabolism, amplifying cannabinoid production, though this is speculative. 4. **Nutrient Efficiency and Pathogen Resistance**: - **Arginine/Citrulline + Glycine/EAAs**: Arginine and citrulline improve nitrogen use efficiency, complementing glycine’s chelating properties and EAAs’ nutrient uptake enhancement, optimizing cannabis growth in hydroponics or nutrient-poor soils. - **MB Synergy**: MB’s antimicrobial properties may synergize with NO’s role in plant defense signaling, protecting cannabis from pathogens like *Botrytis* or *Pythium*. ### Potential Risks and Considerations - **Nitrogen Overload**: The NO capsule adds arginine, citrulline, and beta-alanine, all nitrogen-containing compounds, to an already nitrogen-rich mix (EAAs, glutamine, glycine). This increases the risk of nitrogen toxicity in cannabis, causing dark green leaves, clawing, or reduced flowering. Dilute to 100–500 mg/L total and monitor plant response. - **Absorption Competition**: Arginine, citrulline, EAAs, glutamine, and glycine may compete for root or foliar uptake, reducing efficacy. Stagger applications (e.g., NO capsule components in the morning, others in the evening) or alternate days. - **MB Interaction**: MB’s redox activity may interact with NO from arginine/citrulline, potentially amplifying or disrupting signaling if doses are unbalanced. Keep MB at ~1 mg/L (e.g., two drops of 1% solution per liter). - **Beta-Alanine Limited Impact**: At 40 mg, beta-alanine’s effects may be negligible, as plant studies use higher concentrations (e.g., 100–500 mg/L) for measurable benefits. - **Toxicity Risk**: High doses of arginine or citrulline (1000 mg/L) may disrupt nitrogen balance, while MB (3 mg/L) could cause photodynamic damage. Test low doses first. - **Cultivar Variability**: Sativa-dominant cannabis may benefit more from stress mitigation (arginine, citrulline, proline, TMG, MB), while indicas may respond better to growth promotion (EAAs, glutamine, glycine). ### Practical Recommendations - **Dosing**: - NO Capsule: Dissolve one capsule (740 mg total: 200 mg L-arginine, 200 mg arginine AKG, 200 mg L-citrulline HCl, 100 mg L-citrulline malate, 40 mg beta-alanine) in 2–10 L water (~74–370 mg/L). Start at 100–200 mg/L for foliar or root application. - EAAs: 150–300 mg/L - Glutamine: 250–500 mg/L - Proline: 50–100 mg/L - TMG: 0.5–1 g/L - Glycine: 100–500 mg/L - MB: ~1 mg/L (e.g., two drops of 1% solution per liter) - Apply twice weekly during vegetative growth or early flowering. - **Application**: - **Foliar Spray**: Apply during low-light periods (e.g., early morning) to avoid MB phototoxicity. Combine NO capsule components with amino acids for balanced uptake. - **Root Drench**: Suitable for hydroponics or soil, ensuring low MB concentrations to avoid microbial disruption. - **Staggering**: Apply NO capsule + MB separately from amino acids/TMG to minimize uptake competition (e.g., NO/MB in morning, others in evening). - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener leaves, robust growth). Test on a few plants first, especially for MB and NO components, due to limited cannabis data. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. - **Cultivation Context**: - **Hydroponics**: Use lower doses (~100 mg/L NO capsule, 0.5 mg/L MB) to avoid nutrient lockout or microbial imbalance. - **Soil**: Maintain healthy microbiota, as MB and high nitrogen inputs could disrupt beneficial microbes. - **Cultivars**: Adjust based on strain needs (e.g., stress-prone sativas vs. nutrient-hungry indicas). ### Evidence Gaps - **No Specific Studies**: No research addresses this exact combination (NO capsule + EAAs, proline, TMG, glutamine, glycine, MB) in cannabis. Effects are inferred from individual compound studies in plants (e.g., arginine for NO, MB in algae). - **Cannabinoid/Terpene Impact**: NO and MB may enhance secondary metabolism, but direct effects on THC/CBD or terpenes are unconfirmed. Cannabis-specific trials are needed. - **Beta-Alanine Role**: At 40 mg, beta-alanine’s impact is likely minimal, as plant studies use higher doses. - **Long-Term Effects**: Long-term impacts of MB and NO precursors on cannabis growth or soil health are unknown. Short-term use appears safe at low doses. ### Conclusion Adding a nitric oxide capsule (L-arginine, arginine AKG, L-citrulline, citrulline malate, beta-alanine) to EAAs, proline, TMG, glutamine, glycine, and MB likely enhances cannabis growth, stress tolerance, and nutrient efficiency by boosting NO signaling, nitrogen metabolism, and antioxidant defenses. The mix may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. However, nitrogen overload, absorption competition, and MB’s phototoxicity require careful dosing (e.g., 100–200 mg/L NO capsule, 1 mg/L MB). Test on a small scale, monitor closely, and consult a cannabis cultivation expert for optimization. If you provide the application volume (e.g., liters of water), specific strain, or growth stage, I can refine dosing further. Want me to search for studies on NO precursors in plants or focus on a specific aspect (e.g., NO’s role in cannabinoid synthesis)? 7/29 345am!!🚨. Adding methylene blue (MB) to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, and L-glycine for cannabis plants introduces a compound with unique properties that may influence plant physiology, primarily through its roles as a redox agent, photosensitizer, and potential biostimulant. While methylene blue is more commonly studied in medical and aquatic contexts, its use in plant cultivation, including cannabis, is less documented, and specific studies on this exact combination are absent. Below, I’ll outline the potential effects of methylene blue on cannabis plants, its interactions with the other compounds, and the likely outcomes, drawing from available plant science knowledge and inferred mechanisms. Given the novelty of MB in this context, recommendations are cautious and speculative where direct evidence is lacking. Effects of Methylene Blue on Cannabis Plants Methylene blue (C16H18ClN3S) is a synthetic dye with redox, antimicrobial, and photosensitizing properties. Its effects on plants depend on concentration, application method, and environmental conditions: * Antioxidant and Redox Activity: At low concentrations (e.g., 0.1–10 µM or ~0.03–3 mg/L), MB acts as a redox mediator, scavenging reactive oxygen species (ROS) and protecting cells from oxidative stress caused by drought, high light, or salinity. In cannabis, this could enhance stress tolerance, particularly in high-intensity indoor grows or outdoor environments with temperature fluctuations. * Photosynthesis Enhancement: MB can act as an electron carrier in photosynthetic pathways, potentially improving electron transport in photosystem II. Studies on algae and some plants suggest low doses (e.g., 1–5 µM) may boost chlorophyll fluorescence and photosynthetic efficiency, potentially increasing cannabis biomass and bud quality. * Antimicrobial Effects: MB has antifungal and antibacterial properties, which could protect cannabis roots or foliage from pathogens like Pythium or Botrytis (bud rot). This is particularly relevant in hydroponic systems or humid environments. * Stress Mitigation: MB may stabilize cellular membranes under stress, similar to proline and TMG, potentially reducing damage from environmental extremes. * Potential Toxicity at High Doses: At higher concentrations (10–50 µM or ~3–15 mg/L), MB can generate ROS via photodynamic action under light, causing oxidative damage to plant tissues. This makes precise dosing critical. Application Notes: Two drops of MB (assuming a standard 1% solution, ~10 mg/mL, where 1 drop ≈ 0.05 mL) in, e.g., 1 liter of water yields ~1 mg/L (3 µM), a low concentration likely safe for foliar or root application. However, exact dosing depends on solution strength and volume. Synergistic Effects with EAAs, Proline, TMG, Glutamine, and L-Glycine Adding MB to the existing mix may enhance or modulate the effects on cannabis plants: 1. Enhanced Stress Resilience: * MB + Proline/TMG/Glycine: Proline, TMG, and glycine act as osmoprotectants and antioxidants, while MB scavenges ROS and stabilizes redox balance. Together, they could significantly improve cannabis tolerance to drought, heat, or salinity, reducing leaf wilting or nutrient stress in challenging environments (e.g., outdoor grows or high-EC hydroponics). * Glutamine/EAAs Synergy: Glutamine and EAAs support glutathione production, which complements MB’s antioxidant effects, potentially amplifying protection against oxidative stress from high light or nutrient imbalances. 2. Growth and Biomass: * MB + EAAs/Glutamine/Glycine: EAAs, glutamine, and glycine drive protein synthesis and nitrogen assimilation, while MB’s potential to enhance photosynthesis could provide the energy needed for these processes. This may lead to increased vegetative growth, larger leaves, and potentially denser buds in cannabis. * Proline Contribution: Proline supports tissue integrity, complementing MB’s membrane-stabilizing effects, which could enhance overall plant vigor. 3. Secondary Metabolite Production: * MB + Glutamine/TMG/Glycine: MB’s role as a photosensitizer may influence metabolic pathways by altering redox states, potentially enhancing synthesis of cannabinoids (e.g., THC, CBD) or terpenes. Glutamine and glycine contribute to precursor pathways (e.g., GABA, purines), while TMG’s methyl donation supports enzyme activity. This combination could theoretically increase terpene diversity or cannabinoid yield, though direct evidence is lacking. * EAAs Role: Methionine (an EAA) and glycine may support sulfur-based terpenes, with MB potentially amplifying metabolic efficiency via improved photosynthesis. 4. Nutrient and Pathogen Management: * MB + Glycine/EAAs: Glycine’s chelating properties and EAAs’ nutrient uptake enhancement could work with MB’s antimicrobial effects to improve nutrient availability and protect roots from pathogens in hydroponic or soil systems. * TMG/Glutamine: TMG’s cellular hydration and glutamine’s role in nitrogen metabolism may enhance MB’s ability to maintain cellular function under stress, improving overall nutrient efficiency. Potential Risks and Considerations * Toxicity Risk: MB at concentrations 10 µM (~3 mg/L) can produce ROS under light, damaging chloroplasts or cell membranes. Two drops in 1 liter (~1 mg/L) is likely safe, but overuse or concentrated solutions could harm cannabis, causing leaf burn or stunted growth. * Absorption Competition: MB may interact with amino acids during uptake, potentially reducing the efficacy of EAAs, glutamine, or glycine if applied simultaneously. Stagger applications (e.g., MB foliar spray, amino acids via root drench) to minimize this. * Nitrogen Overload: Adding MB to an already nitrogen-rich mix (EAAs, glutamine, glycine) increases the risk of toxicity, especially in cannabis, which is sensitive to excess nitrogen. Monitor for dark green leaves, clawing, or reduced flowering. * Photodynamic Effects: MB’s photosensitizing properties require careful application timing. Apply during low-light periods (e.g., early morning or evening) to avoid ROS generation in high-intensity grow lights. * Limited Cannabis Data: MB’s effects are mostly studied in algae, bacteria, or medical contexts, not cannabis. Its benefits are speculative, and plant-specific responses may vary by cultivar (e.g., sativa vs. indica). Practical Recommendations * Dosing: * MB: Two drops of 1% MB solution (~1 mg/L or 3 µM) per liter of water for foliar spray or root drench. Start with 1 drop/L to test tolerance. * EAAs: 150–300 mg/L * Glutamine: 250–500 mg/L * Proline: 50–100 mg/L * TMG: 0.5–1 g/L * Glycine: 100–500 mg/L * Apply twice weekly during vegetative growth or early flowering, adjusting based on plant response. * Application: * Foliar Spray: Apply MB and amino acids in low-light conditions to avoid phototoxicity. Use a fine mist to cover leaves evenly. * Root Drench: Suitable for hydroponics or soil, ensuring MB is diluted to avoid root damage. * Stagger Applications: Apply MB separately from amino acids (e.g., MB in the morning, amino acids in the evening) to reduce uptake competition. * Monitoring: Check for signs of stress relief (greener leaves, faster growth) or toxicity (yellowing, wilting). Test on a few plants first, especially for MB, as cannabis sensitivity is unknown. * Synergy with Nutrients: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. * Cultivation Context: * Hydroponics: Use lower MB doses (~0.5 mg/L) to avoid nutrient lockout or microbial disruption in sterile systems. * Soil: Ensure healthy soil microbiota, as MB’s antimicrobial effects could impact beneficial microbes if overused. * Cultivars: Sativa-dominant strains may benefit more from MB’s stress mitigation, while indicas may respond better to amino acid-driven growth. Evidence Gaps * No Specific Studies: No research addresses MB combined with EAAs, proline, TMG, glutamine, and glycine in cannabis. MB’s effects are extrapolated from algae, bacteria, or limited plant studies (e.g., Arabidopsis, lettuce). * Cannabinoid/Terpene Impact: MB’s influence on secondary metabolites is speculative. Its redox and photosynthetic effects may enhance terpene or cannabinoid synthesis, but cannabis-specific data are needed. * Long-Term Effects: MB’s long-term impact on cannabis growth, soil microbiota, or bud quality is unknown. Short-term studies suggest low doses are safe, but chronic use requires caution. * X Search Results: No recent posts on X discuss MB in cannabis cultivation with this combination. General posts mention MB for water treatment or medical uses, not plant applications. Conclusion Adding two drops of methylene blue (~1 mg/L) to a mix of EAAs, proline, TMG, glutamine, and glycine likely enhances cannabis stress tolerance, photosynthetic efficiency, and pathogen resistance, complementing the growth-promoting and nutrient-uptake benefits of the amino acids and TMG. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) effects on cannabinoid and terpene production. However, MB’s photodynamic and antimicrobial properties require precise dosing and application timing to avoid toxicity or microbial disruption. Start with low doses, test on a small scale, and monitor closely, consulting a cannabis cultivation expert for optimization. If you provide the MB solution concentration, application volume, or specific cannabis strain, I can refine dosing recommendations. Want me to search again for MB-related plant studies or focus on a specific aspect (e.g., MB’s photosynthetic effects)?""""""🚨 8/1 245am L-GLYCINE AND Mg-Zyme by Biotics Research... LIKE NON DRUG TRANQ...KNOCKED ME OUT FOR 2 HOURS..WOW. VERY NICE!!!! 10am * NO Capsule: Dissolve one capsule (740 mg total: 200 mg L-arginine, 200 mg arginine AKG, 200 mg L-citrulline HCl, 100 mg L-citrulline malate, 40 mg beta-alanine) in 2–10 L water (~74–370 mg/L). Start at 100–200 mg/L for foliar or root application. * EAAs: 150–300 mg/L * Glutamine: 250–500 mg/L * Proline: 50–100 mg/L * TMG: 0.5–1 g/L * Glycine: 100–500 mg/L * MB: ~1 mg/L (e.g., two drops of 1% solution per liter) 🚨HALF STRENGTH OF THIS TODAY WITH 4ML PER L CON GROW, PH 5.8. 8/1 935pm HOLY SILT!!! EEA'S 1/4 TSP - PROLINE 1/4ish TSP....some Methylene Blue, NO pill, feels JUST RIGHT.... Last night L Glycine and Mg-Zyne triple magnesium from Biotics Research ..... it's working on ME!!! I look like a walking skeleton with huge veins popping out, really gross I should be dead already but I have unusually high ability for healing and self repair. I am capable of a Medical stasis meditation that can last for a few hours at a time, it's like sleeping but only parts of me are allowed to sleep and heal....very very very complicated. 8/3 430am lights down to 100ish from 108 up from the original 93, which is the break-even wattage where they dont really produce much heat, not more than me standing in the room.